Chemical Analysis and Antioxidant Activities In Vitro of Polysaccharide Extracted from Corn Silk

2012 ◽  
Vol 535-537 ◽  
pp. 2335-2339 ◽  
Author(s):  
Yu Tang He ◽  
Hong Ni Gao ◽  
Yong Xia Xu ◽  
Xiao Ming Pan ◽  
Jian Rong Li
Keyword(s):  
Chemical Analysis ◽  
Hydroxyl Radical ◽  
Column Chromatography ◽  
Antioxidant Activities ◽  
Chemical Properties ◽  
Corn Silk ◽  
Scavenging Capacity ◽  
Glucosidic Bond ◽  
Cellulose Column

Corn silk polysaccharide (CSP) was investigated for the treatment of different kinds of diseases. In order to characterize the chemical properties and antioxidant activities of CSP, the CSP was isolated from corn silk and purified by DE-52 cellulose column chromatography. Four components were separated, and the highest one named CSP-A. The CSP-A was characterized by FTIR and the monosaccharide components were analyzed by HPLC. The FTIR spectra indicated that CSP-A was characteristic of β-glucosidic bond and α-glycosidic bond. The CSP-A mainly comprised of glucose, galactose, arabinose, mannose, rhamnose. The antioxidant activities of CSP were determined by hydroxyl radical (•OH) and DPPH radicals scavenging assays. When the concentration of CSP was 10mg/mL, the scavenging capacity of •OH and DPPH could reach to 40% and 48%, respectively.

scholarly journals Extraction Optimization of Polysaccharides From Corn Silk and Their Antioxidant Activities in vitro and in vivo

2021 ◽  
Vol 12 ◽  
Author(s):  
Liang Zhang ◽  
Yang Yang ◽  
Zhanyong Wang
Keyword(s):  
Antioxidant Activities ◽  
Dry Weight ◽  
Extraction Time ◽  
Solid Ratio ◽  
Corn Silk ◽  
Response Surface Technique ◽  
Experimental Yield ◽  
Temperature Liquid

Response surface technique was employed for improving the extraction of corn silk polysaccharides (CSP). Temperature, liquid-to-solid ratio, and per extraction time were all examined as separate factors. The optimal extraction parameters were determined by fitting experimental data to a second-order polynomial; a liquid-to-solid ratio of 21.5 ml/g, temperature equivalent to 88°C, and extraction time of 1.87 h. The experimental yield of the extracted polysaccharides following the application of these conditions was 4.33 ± 0.08% (dry weight), which fit quite well with the predicted value. CSP’s strong scavenging capabilities against hydroxyls, 1,1-diphenyl-2-picrylhydrazyl radicals, and superoxide anions along with its excellent reducing potential, were demonstrated in an in vitro antioxidant experiment. Meanwhile, in vivo testing revealed that CSP substantially enhanced glutathione peroxidase and superoxide dismutase activities. The Malondialdehyde levels in the liver and serum of aged mice also underwent a decrease. This study found that CSP has a substantial antioxidant potential in vitro and in vivo, suggesting that it might be used as an antioxidant in food and medicine.

scholarly journals IN VITRO STUDY OF PHYSICO-CHEMICAL PROPERTIES OF THE «BALANCED SALT SOLUTION − H2O» SYSTEM WITH THE PURPOSE OF DEVELOPMENT OF TREATMENT, REHABILITATION AND PREVENTIVE MEASURES IN OPHTHALMOLOGY

2021 ◽  
Author(s):  
O. Kofanova ◽  
К. Tkachuk ◽  
O. Kofanov ◽  
M. Saveliev ◽  
O. Tverda ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Mathematical Models ◽  
Salt Solution ◽  
Dynamic Viscosity ◽  
Preventive Measures ◽  
Chemical Properties ◽  
In Vitro Study ◽  
Vitro Study ◽  
Physico Chemical

The research is devoted to the in vitro study of the behavior of the model system «irrigation balanced salt solution BSS − H2O» in order to further develop treatment, rehabilitation and prevention measures for patients with ophthalmic diseases. The aim of the investigation is the experimental determination and analysis of physico-chemical properties of irrigated balanced salt solution, the study of its behavior in the aquatic environment to model changes in the characteristics of intraocular fluid and further development of rehabilitation and preventive measures for ophthalmic patients. Materials and Methods. The study used methods of physico-chemical analysis, in particular, densimetry and viscosimetry of model systems containing BSS for intraocular use and double-distilled water in different volume ratios. The density of the system was determined pycnometrically (20.00 0С ± 0.05 0С; 101.3 kPa); the correction for weight loss of bodies in the air has been taken into account. Viscosity was measured under the same conditions with an Ostwald viscometer. Statistical analysis and evaluation of the reliability of the results were performed using such software products as MS Excel 2007, Google Spreadsheets, SPSS Statistics. Results. Approximate mathematical models of the dependences of density and kinematic and dynamic viscosity on the composition of the multicomponent system «BSS – H2O» were obtained with quite high coefficients of determination. Statistical significance and adequacy of model selection were tested by Student's criterion at a significance level of 5 %. To develop therapeutic, prophylactic and rehabilitation measures, mathematical models of kinematic and dynamic viscosity dependences on the density of the «BSS – H2O» system, measured under the same conditions, were built. The models also have high coefficients of determination. Conclusion. The in vitro physico-chemical analysis of the system «BSS – H2O», as well as the obtained approximate mathematical models can be used to predict possible changes in the characteristics of irrigated balanced salt solution during its long stay in the patient's eye.

Antioxidant Activities of Flavonoids from Canavalia maritime

2013 ◽  
Vol 834-836 ◽  
pp. 577-581
Author(s):  
Jian Chao Deng ◽  
Gai Gai Niu ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
Yong Chuan Deng ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Hydroxyl Radical ◽  
High Speed ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Counter Current

Flavonoids was isolated from canavalia maritime by accelerated solvent extraction (ASE) coupled with high-speed counter-current chromatography (HSCCC). The antioxidant activities of flavonoids were investigated in vitro and evaluated by IC50. The results indicated that flavonoids possessed obvious reducing power and significant inhibitory effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. These results suggest that canavalia maritime flavonoids could be a suitable natural antioxidant for humans.

Chemical analysis and antioxidant activities in vitro of polysaccharide extracted from Opuntia ficus indica Mill. cultivated in China

2010 ◽  
Vol 82 (3) ◽  
pp. 722-727 ◽  
Author(s):  
Xian-Ke Zhong ◽  
Xin Jin ◽  
Feng-Ying Lai ◽  
Qing-Sheng Lin ◽  
Jian-Guo Jiang
Keyword(s):  
Chemical Analysis ◽  
Antioxidant Activities ◽  
Opuntia Ficus Indica

scholarly journals Antioxidant Activity and Mechanism of Protocatechuic Acid in vitro

2011 ◽  
Vol 1 (7) ◽  
pp. 232 ◽  
Author(s):  
Xican Li ◽  
Xiaozhen Wang ◽  
Dongfeng Chen ◽  
Shuzhi Chen
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chelating Ability ◽  
Positive Controls

Background: Protocatechuic acid (PCA) is a natural phenolic acid widely distributed in plants and is considered as an active component of some traditional Chinese herbal medicines such as Cibotium barometz (L.) J.Sm, Stenoloma chusanum (L.) Ching, Ilex chinensis Sims. PCA was reported to possess various pharmacological effects which may be closely correlated with its antioxidant activities. However, the antioxidant of PCA has not been investigated systematically yet. Methods: In the study, the antioxidant activities of protocatechuic acid were measured in vitro using various antioxidant assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH•), 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS+•), superoxide anion radicals (•O2-) and hydroxyl radical (•OH) scavenging activity, ferric ions (Fe3+) and cupric ions (Cu2+) reducing power, ferrous ions (Fe2+) and cupric ions (Cu2+) chelating activity, compared with the positive controls Trolox or BHT.Results: In all assays, PCA along with positive controls exhibited dose-dependently antioxidant ability. Comparing to a standard antioxidant Trolox, the relative antioxidant activity of PCA (i.e. the ratio of IC50(Trolox)/IC50(PCA) ) was calculated as 2.8, 2.3, 3.7, 6.1, 4.2, 1.0, 2.7, 1.5, respectively, for DPPH, ABTS, reducing power (Fe3+), reducing power (Cu2+), superoxide anion radical-scavenging, hydroxyl radical-scavenging, chelating ability (Fe2+) and chelating ability (Cu2+). Conclusion: Comparing to Trolox, PCA shows much more effective antioxidant activity in vitro in both lipid and aqueous media. Hence, it could therefore be used in pharmacological or food industry as a natural antioxidant. It may exhibit antioxidant activity by both chelating metal transition ions as well as by scavenging free radicals via donating hydrogen atom (H•) or electron (e).Keywords: Protocatechuic acid, antioxidant, reducing power, free radical-scavenging, chelating ability.

scholarly journals Trypsin-hydrolyzed Corn Silk Proteins: Antioxidant Activities, in vitro Gastrointestinal and Thermal Stability, and Hematoprotective Effects

eFood ◽  
2020 ◽  
Author(s):  
Tsun-Thai Chai ◽  
Shin-Yii Ang ◽  
Kervine Goh ◽  
You-Han Lee ◽  
Jia-Min Ngoo ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activities ◽  
Silk Proteins ◽  
Corn Silk

scholarly journals Partial purification of neurofilament subunits from bovine brains and studies on neurofilament assembly.

1981 ◽  
Vol 89 (3) ◽  
pp. 560-567 ◽  
Author(s):  
H M Moon ◽  
T Wisniewski ◽  
P Merz ◽  
J De Martini ◽  
H M Wisniewski
Keyword(s):  
Column Chromatography ◽  
High Speed ◽  
Bovine Brain ◽  
Urea Solution ◽  
Partial Purification ◽  
Filament Formation ◽  
Globular Structure ◽  
Backbone Structure ◽  
Cellulose Column

The 200,000-dalton neurofilament subunit (P200) and the 160,000-dalton (P160) and 78,000-dalton (P78) neurofilament subunits were partially purified from bovine brain. Intact neurofilaments were prepared by high-speed and sucrose-zone centrifugation. The crude neurofilament was solubilized in 8 M urea solution containing pyridine, formic acid, and 2-mercaptoethanol. The solubilized neurofilament was purified by carboxymethyl (CM) cellulose column and hydroxylapatite column chromatography. The P200 was purified as separate from P160 and P78, but the P160 and P78 subunits were copurified on CM cellulose, hydroxylapatite, Bio-Gel A150m, and Sephadex G-150 column chromatography. Electron microscopy of these purified neurofilament subunits revealed the P200 subunit as a globular structure, and the P160 and P78 subunits as a rod-shaped structure extending up to 120 nm with a 8- to 12-nm width. In the presence of 200 mM KCl, 15 mM MgCl2, and 1 mM ATP, the purified subunits assembled into long filaments. Under the assembly condition, P160 and P78 subunits elongated up to 500 nm, but the longer filament formation required the presence of P200 subunits. The filaments formed in vitro were of two types: long straight filaments and intertwined knobby-type filaments. From these results, we have suggested that P160 and P78 form the neurofilament backbone structure and P200 facilitates the assembly of the backbone units into longer filaments.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

An in vitro study of the peroxyl and hydroxyl radical scavenging capacity of the calcium antagonist amlodipine

2004 ◽  
Vol 58 (8) ◽  
pp. 423-426 ◽  
Author(s):  
Ferdinando Franzoni ◽  
Gino Santoro ◽  
Francesco Regoli ◽  
Yvonne Plantinga ◽  
Francesca R. Femia ◽  
...  
Keyword(s):  
Calcium Antagonist ◽  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Vitro Study ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Modification of Zein and its Antioxidant Property In Vitro

2014 ◽  
Vol 1004-1005 ◽  
pp. 864-867 ◽  
Author(s):  
Yu Tang He ◽  
Tian Gang Yin ◽  
Yu Mei Xie ◽  
Yong Xia Xu ◽  
He Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Property ◽  
Practical Significance ◽  
Orthogonal Experimental Design ◽  
Deep Processing ◽  
Fracture Elongation ◽  
Optimum Parameters ◽  
Scavenging Capacity

Modified zein could improve functional properties of zein broadening its application. The L9(34) orthogonal experimental design was applied to optimize modification technology of zein. The results showed that the optimum parameters of zein modification were using gallic acid as modifier, ethanol 80% and modification time 2.0h. Under these conditions, the fracture elongation of modified zein was reached to 79.5%. The results showed obvious effects. The antioxidant activities of zein were determined by •OH and DPPH radicals scavenging assays. When modified zein concentration was 2.0 g/mL the scavenging capacity of DPPH and •OH reached to 34.7% and 45.2%. The antioxidant activity of zein was less than vitamin C. This study has important practical significance for deep processing of zein resources.

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