Optimization of the Heterologous Expression of Sesuvium portulacastrum Betaine Aldehyde Dehydrogenase in Escherichia coli

A full-length sequence coding for a betaine aldehyde dehydrogenase gene from S. portulacastrum was cloned into expression vector pGEX-4T-1, and named pGEX-4T-SpBADH. The GST-SpBADH fusion protein was expressed and the expression conditions were optimized. Through the research on optimization of expression the concentration of IPTG, concentration of bacterium, induction time and temperature and so on, the results showed, the expression of GST-SpBADH increased accompany with the induction time. The expression level of GST-SpBADH fusion protein reached the highest for 5 h cultured and for OD600 is about 0.6 at 37°C, 0.2 mmol/L IPTG can effectively induce the expression of GST-SpBADH in Escherichia coli expression system.