scholarly journals Effect of Low Temperature Pre-Treatment on Anther Culture in Selected Indica, Japonica Rice Varieties and their Inter Sub-Specific Hybrids

2009 ◽  
Vol 38 (1) ◽  
pp. 11 ◽  
Author(s):  
HNI Herath ◽  
DC Bandara ◽  
PK Samarajeewa ◽  
DSA Wijesundara
Keyword(s):  
Low Temperature ◽  
Anther Culture ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Pre Treatment

scholarly journals Global analysis of differentially expressed genes between two Japonica rice varieties induced by low temperature during the booting stage by RNA-Seq

2020 ◽  
Vol 7 (6) ◽  
pp. 192243
Author(s):  
Zhenhua Guo ◽  
Lijun Cai ◽  
Chuanxue Liu ◽  
Cuihong Huang ◽  
Zhiqiang Chen ◽  
...  
Keyword(s):  
Low Temperature ◽  
Differentially Expressed Genes ◽  
Chilling Stress ◽  
Cold Treatment ◽  
Chilling Tolerance ◽  
Differentially Expressed ◽  
Antioxidant Enzyme Activities ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Booting Stage

As one of the abiotic stresses, low temperature severely threatens rice production during its entire growth period, especially during the booting stage. In the present study, transcriptome analysis was performed comparing Longjing (LJ) 25 (chilling-tolerant) and LJ 11 (chilling-sensitive) rice varieties to identify genes associated with chilling tolerance in rice spikelets. A total of 23 845 expressed genes and 13 205 differentially expressed genes (DEGs) were identified, respectively. Gene ontology (GO) enrichment analyses revealed ‘response to cold’ (containing 180 DEGs) as the only category enriched in both varieties during the entire cold treatment period. Through M ap M an analysis, we identified nine and six DEGs related to the Calvin cycle and antioxidant enzymes, respectively, including OsRBCS3 , OsRBCS2 , OsRBCS4 , OsAPX2 and OsCATC , that under chilling stress were markedly downregulated in LJ11 compared with LJ25. Furthermore, we predicted their protein–protein interaction (PPI) network and identified nine hub genes (the threshold of co-expressed gene number ≥ 11) in Cytoscape, including three RuBisCO-related genes with 14 co-expressed genes. Under chilling stress, antioxidant enzyme activities (peroxidase (POD) and catalase (CAT)) were downregulated in LJ11 compared with LJ25. However, the content of malondialdehyde (MDA) was higher in LJ11 compared with LJ25. Collectively, our findings identify low temperature responsive genes that can be effectively used as candidate genes for molecular breeding programmes to increase the chilling tolerance of rice.

scholarly journals Breeding for Low Temperature Germinability in Temperate Japonica Rice Varieties: Analysis of Candidate Genes in Associated QTLs

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2125
Author(s):  
Ester Sales ◽  
Eva Miedes ◽  
Luis Marqués
Keyword(s):  
Low Temperature ◽  
Genome Wide Association Study ◽  
Recombinant Inbred Lines ◽  
Rice Chromosome ◽  
Temperature Tolerance ◽  
Chromosome 3 ◽  
Japonica Rice ◽  
Rice Cultivars ◽  
Rice Varieties ◽  
Low Temperature Tolerance

In temperate areas, rice deals with low temperatures that can affect plant growth and crop yield. Rapid germination is required for adequate plant establishment in the field, therefore obtaining cultivars that maintain this phenotype under suboptimal temperature conditions is a challenge for rice breeders. Our study aimed to investigate temperature-induced expression changes in genes underlying quantitative trait loci (QTLs) associated to this trait (low temperature germinability, LTG) that were detected in a previous genome wide association study (GWAS). In the context of a breeding program for japonica rice cultivars adapted to cultivation in Spain, we obtained two biparental families of lines derived from hybridization with two cold tolerant Italian cultivars, and we have studied the effect on the LTG phenotype of introgressing these QTLs. A wide region in chromosome 3 was related to significant increases in seedling growth rate at 15 °C, although the extent of the effect depended on the analyzed family. In parallel, we studied the pattern of expression during germination at different temperatures of 10 genes located in the LTG-associated QTLs, in five japonica rice cultivars and in a biparental family of recombinant inbred lines (RILs). Cold induced changes in the expression of the 10 analyzed genes, with significant differences among genotypes. Variation in LTG phenotype was consistently associated with changes in the pattern of expression of five genes from the tagged regions in rice chromosome 3, which encoded for enzymes implicated in phytohormone metabolism (OsFBK12, Os3Bglu6), oxidative stress (SPL35, OsSRO1c) and Mn homeostasis maintenance (OsMTP8.1). Differential expression induced by cold in two regulatory genes (Os02g0824000 and Os06g06400) also contributed to explain low temperature tolerance during rice germination. In conclusion, introgression in defective cultivars of favorable alleles for these genes would contribute to the genetic improvement of LTG in japonica rice varieties.

scholarly journals Genotypic Variation in Nutrient Uptake Requirements of Rice Using the QUEFTS Model

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 26
Author(s):  
Tao Sun ◽  
Xin Yang ◽  
Sheng Tang ◽  
Kefeng Han ◽  
Ping He ◽  
...  
Keyword(s):  
Nutrient Uptake ◽  
Field Experiments ◽  
Hybrid Rice ◽  
Genotypic Variation ◽  
Tropical Soils ◽  
Study Data ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Indica Hybrid Rice ◽  
Validation Experiments

Nutrient requirements for single-season rice using the quantitative evaluation of the fertility of tropical soils (QUEFTS) model in China have been estimated in a previous study, which involved all the rice varieties; however, it is unclear whether a similar result can be obtained for different rice varieties. In this study, data were collected from field experiments conducted from 2016 to 2019 in Zhejiang Province, China. The dataset was separated into two parts: japonica/indica hybrid rice and japonica rice. To produce 1000 kg of grain, 13.5 kg N, 3.6 kg P, and 20.4 kg K were required in the above-ground plant dry matter for japonica/indica hybrid rice, and the corresponding internal efficiencies (IEs) were 74.0 kg grain per kg N, 279.1 kg grain per kg P, and 49.1 kg grain per kg K. For japonica rice, 17.6 kg N, 4.1 kg P, and 23.0 kg K were required to produce 1000 kg of grain, and the corresponding IEs were 56.8 kg grain per kg N, 244.6 kg grain per kg P, and 43.5 kg grain per kg K. Field validation experiments indicated that the QUEFTS model could be used to estimate nutrient uptake of different rice varieties. We suggest that variety should be taken into consideration when estimating nutrient uptake for rice using the QUEFTS model, which would improve this model.

scholarly journals A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture

Euphytica ◽  
2013 ◽  
Vol 195 (1) ◽  
pp. 31-44 ◽  
Author(s):  
X. Serrat ◽  
M. Cardona ◽  
J. Gil ◽  
A. M. Brito ◽  
L. Moysset ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Japonica Rice ◽  
Cultivar Improvement

scholarly journals Colchicine and osmotic stress for improving anther culture efficiency on long grain temperate and tropical japonica rice genotypes

2019 ◽  
Vol 36 (4) ◽  
pp. 269-273
Author(s):  
Irene Ferreres ◽  
Mirari Ortega ◽  
Camilo López-Cristoffanini ◽  
Salvador Nogués ◽  
Xavier Serrat
Keyword(s):  
Osmotic Stress ◽  
Anther Culture ◽  
Japonica Rice ◽  
Tropical Japonica ◽  
Rice Genotypes
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