scholarly journals A fast and efficient method for total DNA extraction from soil filamentous fungi

2014 ◽  
Vol 32 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Wilson Huanca-Mamani ◽  
Ricardo Salvatierra Martínez ◽  
Germán Sepúlveda-Chavera
Keyword(s):  
Dna Extraction ◽  
Filamentous Fungi ◽  
Efficient Method ◽  
Total Dna

Plant Total DNA Extraction

1998 ◽  
pp. 10-14 ◽  
Author(s):  
Julia Rueda ◽  
Rosario Linacero ◽  
Ana M. Vázquez
Keyword(s):  
Dna Extraction ◽  
Total Dna

scholarly journals Small-scale total DNA extraction from bacteria and yeast for PCR applications

2005 ◽  
Vol 347 (2) ◽  
pp. 333-335 ◽  
Author(s):  
José Luis Ruiz-Barba ◽  
Antonio Maldonado ◽  
Rufino Jiménez-Díaz
Keyword(s):  
Dna Extraction ◽  
Small Scale ◽  
Total Dna

scholarly journals An efficient method for DNA extraction from Cladosporioid fungi

2010 ◽  
Vol 9 (4) ◽  
pp. 2283-2291 ◽  
Author(s):  
M.A. Moslem ◽  
A.H. Bahkali ◽  
K.A. Abd-Elsalam ◽  
P.J.G.M. Wit
Keyword(s):  
Dna Extraction ◽  
Efficient Method

scholarly journals Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake

2001 ◽  
Vol 13 (3) ◽  
pp. 285-288 ◽  
Author(s):  
LU Yuan ◽  
◽  
WEN Jianfan ◽  
LV Tianwen
Keyword(s):  
Dna Extraction ◽  
Microcystis Aeruginosa ◽  
Dianchi Lake ◽  
Total Dna

scholarly journals Detection of Badnavirus in pineapple in northeastern Brazil

2020 ◽  
Vol 5 (4) ◽  
pp. 2450-2463
Author(s):  
Edlene Maria da Silva Moraes Santos ◽  
Jaqueline Figueredo de Oliveira Costa ◽  
Mayra Machado de Medeiros Ferro ◽  
Sarah Jacqueline Cavalcanti da Silva ◽  
Iraildes Pereira Assunção ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Distinct Species ◽  
Northeastern Brazil ◽  
Ananas Comosus ◽  
Pairwise Comparisons ◽  
Sequencing Analysis ◽  
First Report ◽  
Northeastern Region ◽  
Total Dna

ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.

scholarly journals An Efficient Method for Genomic DNA Extraction from Different Molluscs Species

2011 ◽  
Vol 12 (11) ◽  
pp. 8086-8095 ◽  
Author(s):  
Jorge C. Pereira ◽  
Raquel Chaves ◽  
Estela Bastos ◽  
Alexandra Leitão ◽  
Henrique Guedes-Pinto
Keyword(s):  
Dna Extraction ◽  
Efficient Method ◽  
Genomic Dna ◽  
Genomic Dna Extraction
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