scholarly journals A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and hematoxylin and eosin staining

2014 ◽  
Vol 6 (02) ◽  
pp. 084-090 ◽  
Author(s):  
Pinki Pandey ◽  
Alok Dixit ◽  
Aparna Tanwar ◽  
Anuradha Sharma ◽  
Sanjeev Mittal
Keyword(s):  
Staining Method ◽  
The Other ◽  
Staining Procedure ◽  
Significant Difference ◽  
Efficient Alternative ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
The Difference ◽  
Different Tissues

ABSTRACT Introduction: Our study presents a new deparaffinizing and hematoxylin and eosin (H and E) staining method that involves the use of easily available, nontoxic and eco-friendly liquid diluted dish washing soap (DWS) by completely eliminating expensive and hazardous xylene and alcohol from deparaffinizing and rehydration prior to staining, staining and from dehydration prior to mounting. The aim was to evaluate and compare the quality of liquid DWS treated xylene and alcohol free (XAF) sections with that of the conventional H and E sections. Materials and Methods: A total of 100 paraffin embedded tissue blocks from different tissues were included. From each tissue block, one section was stained with conventional H and E (normal sections) and the other with XAF H and E (soapy sections) staining method. Slides were scored using five parameters: Nuclear, cytoplasmic, clarity, uniformity, and crispness of staining. Z-test was used for statistical analysis. Results: Soapy sections scored better for cytoplasmic (90%) and crisp staining (95%) with a statistically significant difference. Whereas for uniformity of staining, normal sections (88%) scored over soapy sections (72%) (Z = 2.82, P < 0.05). For nuclear (90%) and clarity of staining (90%) total scored favored soapy sections, but the difference was not statistically significant. About 84% normal sections stained adequately for diagnosis when compared with 86% in soapy sections (Z = 0.396, P > 0.05). Conclusion: Liquid DWS is a safe and efficient alternative to xylene and alcohol in deparaffinization and routine H and E staining procedure. We are documenting this project that can be used as a model for other histology laboratories.

scholarly journals PERBANDINGAN KUALITAS PRODUK DAN MINAT MENABUNG PADA LPD DESA ADAT TAJUN DENGAN DESA ADAT TEGAL

2020 ◽  
Vol 4 (1) ◽  
pp. 59-67
Author(s):  
Eka Swarnadi Luh ◽  
Ketut Budi Susrusa ◽  
Ida Ayu Listia Dewi
Keyword(s):  
Product Quality ◽  
Financial Institutions ◽  
Rapid Development ◽  
Research Data ◽  
The Other ◽  
Physical Evidence ◽  
Significant Difference ◽  
The Difference ◽  
Better Than

LPDs are non-bank financial institutions that are regulated and approved by the Regional Regulations of the Province of Bali. The management of LPD is fully handed over to the relevant Pakraman village. In line with the rapid development of LPDs, it turns out that on the other hand it shows diverse performance, so that LPDs need to pay attention to the level of product quality and customer interest in the products offered.            The purpose of the study was to determine the comparison of product quality and interest in saving at the Tajun Traditional Village LPD with the Traditional Village of Tegal. The number of samples from Tajun Adat Village LPD was 98 people and the LPD of Tegal Traditional Village was 84 people. The research data were analyzed by the Mann-Whitney Test. The results showed that there was a significant difference between the quality of the products of the Adat Village of Tajun LPD and the Traditional Village of Tegal. This difference is indicated by indicators of physical evidence, reliability, responsiveness and empathy. The product quality of Tajun Adat Village's LPD is better than the traditional village of Tegal. There is a significant difference between the interest in saving the traditional village of Tajun LPD and the traditional village of Tegal. The difference is in the indicator of confidence. Interest in Saving Tajun Indigenous Village LPD is higher than the Traditional Village of Tegal.

The partial and total replacement of wheat and soybean meal by pollard in a wheat-soybean meal grower-pig ration

1969 ◽  
Vol 9 (41) ◽  
pp. 594 ◽  
Author(s):  
RM Beames ◽  
WJ Natoli
Keyword(s):  
Soybean Meal ◽  
Protein Quality ◽  
Growing Pigs ◽  
The Other ◽  
Net Energy ◽  
Eye Muscle ◽  
Digestible Energy ◽  
Significant Difference ◽  
The Difference

This experiment was designed to investigate the effect of high levels of pollard in the ration on the growth and carcase quality of growing pigs. Twenty-four gilts and twenty-four male castrate weaner pigs of mean liveweight 23.4 kg were each fed one of four rations in a wet form in individual stalls twice daily until they were slaughtered at a mean liveweight of 86.5 kg. The control ration was 81.9 per cent wheat and 15.0 per cent soybean meal, with added minerals and vitamins. In the other three rations, the wheat and soybean meal portion was replaced by 48.4, 67.8, and 96.9 per cent pollard. Feed allowance was adjusted to give a similar intake of digestible energy for all rations. There was no significant difference between treatments in rate of liveweight gain or in efficiency of utilization of digestible energy. Efficiency of utilization of air-dry feed decreased as level of pollard in the ration increased. These differences were significant except for the difference between the rations containing 48.4 and 67.8 per cent pollard. There was a significant decrease in dressing percentage with each increase in the level of pollard in the ration. Backfat thickness was significantly less in pigs fed rations containing 67.8 and 96.9 per cent pollard than in pigs fed the other two rations. Gilts grew more rapidly, utilized feed and digestible energy more efficiently and produced carcases with greater eye muscle indices and higher appraisal scores than the male castrates. There were no digestive disturbances on any of the rations and all were readily eaten. The results indicate that the relationship between protein quality k d net energy in pollard is of an order normally occurring in well-balanced rations.

Students’ satisfaction with hostel accommodations in higher education institutions

2017 ◽  
Vol 15 (5) ◽  
pp. 652-666 ◽  
Author(s):  
Ayodeji Emmanuel Oke ◽  
Clinton Ohis Aigbavboa ◽  
Marcia M. Raphiri
Keyword(s):  
Higher Education ◽  
Ethnic Groups ◽  
Higher Education Institutions ◽  
Item Score ◽  
Content Type ◽  
Significant Difference ◽  
The Difference ◽  
Higher Institutions ◽  
Students Satisfaction

Purpose There are several types of accommodation available to students of higher education institutions, and it is the responsibility of the institutions to focus on factors that can attract and retain students to their residences. This study aims to investigate satisfaction of higher institutions students with on-campus accommodation with an emphasis on the ethnicity of the students. Design/methodology/approach Using convenience sampling method, questionnaires were administered to students of selected residences in the study area. Mean item score was used to rank identified factors, whereas mean gap and Kruskal–Wallis K-test were adopted to examine the difference in opinion of students from various ethnic groups. Findings Overall, major features that make students dissatisfied with university-owned accommodations are enforcement of rule that compels all students to move out with their belongings during each recess, the effectiveness of the lift system, the size of wardrobe and closet, laundry service in the residence, numbers of electrical sockets and window quality. Considering dwelling unit features, neighbourhood, environmental, building quality and services provided by residence management, this study reveals that there is a significant difference in the satisfaction of students of diverse ethnic groups with their accommodation. Research limitations/implications The study was limited to on-campus residences owned by higher education institutions in South Africa. However, the findings of the study can be adopted for off-campus residences, and such owned by private individuals, agencies and other bodies provided are approved by the institutions. Originality/value The findings of this study will help management of higher education institutions to improve quality of services in their residences for the satisfaction of their students. Developers, contractors and other stakeholders involved in construction of these residences will also find the findings useful in designing and construction of the facilities.

scholarly journals Association of Blood and Urine Parameter Value Change With the Amount of Consumed Water Regime and Sample Sampling in Healthy Individuals

2021 ◽  
Author(s):  
Snezana M Jovicic
Keyword(s):  
Water Intake ◽  
Freezing Point ◽  
Freezing Point Depression ◽  
Sodium Potassium ◽  
Significant Difference ◽  
Urine Biomarker ◽  
Concentration Changes ◽  
The Difference ◽  
And Gender

Abstract Background: Understanding the effect of pre-analytical factors is important for data quality of bio-specimens and health status. The study examines the effect of 9-days fluid intake and 2-time sampling on concentration changes of 7-Urine and 17-Blood variables. Material and Method: SPSS software v23.0 applies to data processing. The group of 23 healthy subjects divide based on water intake and gender. Results: A statistically significant difference(p<0.01) between 1st/2nd sampling is confirmed for Freezing point depression, Sodium, Potassium, Creatinine Urea and Urate in Urine and Urea, Urate, Glucose, Hematocrit, Thrombocyte in Blood. The difference between water intake after 1st sampling is confirmed (p<0.01) for Freezing point depression, Sodium, Urate and(p<0.05) for Potassium(p<0.05), Chloride(p<0.05), Creatinine(p<0.05), Urate, Urea in Urine and Potassium(p<0.01) and Chloride(p<0.05) in Blood. Difference between gender exists for Urea(p<0.05) in Urine after 2nd sampling and Urate(P<0.01), Glucose(p<0.01/0.05), Ht(p<0.01/0.05) after 1st and 2nd sampling and MCHC(p<0.01) after 2nd sampling in Blood samples.Conclusion: Water intake increases blood and urine biomarker range after sampling.

scholarly journals Therapeutic Efficacies of Artemisia capillaris Extract Cream Formulation in Imiquimod-Induced Psoriasis Models

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Song Yi Lee ◽  
Suyeong Nam ◽  
Sungyun Kim ◽  
Ja Seong Koo ◽  
In Kee Hong ◽  
...  
Keyword(s):  
Skin Lesions ◽  
The Other ◽  
Epidermal Thickness ◽  
Artemisia Capillaris ◽  
Cream Formulation ◽  
Cumulative Score ◽  
Intracellular Adhesion Molecule ◽  
Psoriasis Therapy ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

A cream formulation containing Artemisia capillaris (AC) extract (ACE) was developed for psoriasis therapy. Although ACE can be dissolved in organic solvents, its topical application is restricted because of toxicities. Therefore, a cream formulation was developed for the convenient and safe local application of ACE on skin lesions. The antipsoriatic properties of the ACE cream were evaluated using an imiquimod- (IMQ-) induced psoriasis-like mouse model. In psoriasis-like mouse models, the cumulative score (redness, thickness, and scaling) of the IMQ + ACE cream group was significantly lower than those of the other groups on day 4 (p < 0.05). The results of the hematoxylin and eosin staining of skin tissues revealed that the epidermal thickness value of the IMQ + ACE cream group was significantly lower than those of the other experimental groups (p < 0.05). The expression level of intracellular adhesion molecule-1 (ICAM-1), which indicates the leukocyte infiltration into the skin and subsequent interactions with keratinocytes, was also lower in the IMQ + ACE cream group than in the IMQ group. These results indicate that ACE cream formulation could be used safely and conveniently for psoriasis treatment.

The effect of continuous care model-based intervention on the quality of sleep in menopausal women: A clinical trial study

2019 ◽  
Author(s):  
Farzaneh AKBARI ◽  
sousan Heydarpour ◽  
Nader Salari
Keyword(s):  
Clinical Trial ◽  
Intervention Group ◽  
Control Group ◽  
Quality Of Sleep ◽  
Care Model ◽  
Continuous Care ◽  
Menopausal Women ◽  
Significant Difference ◽  
The Difference

Abstract BACKGROUND Sleep disorder, brings in many physical, behavioral, and mental problems. Applying continuous care model leads to proper recognition of the patient’s problems and involves the patient in solving health problems. This study aimed to determine the effect of continuous care model on the quality of sleep in menopausal women. METHODS AND MATERIALS A random clinical trial study was carried out with participation of 110 menopausal women visiting Kermanshah-based clinics (the west of Iran) in 2017. The participants were randomly assigned to intervention (n=55) and control (n=55) groups. The control group received the routine cares and in addition to the routine cares the intervention group attended four weekly group consultation sessions (60-90min). The quality of sleep in the two groups was assessed using Pittsburg Sleep Quality Index before, immediately after, and one month after the intervention. Data analyses were done using independent t-test, ANOVA with frequent measures, Friedman’s test, Wilcoxon’s post hoc test, and X2 test in SPSS (24). RESULTS The mean scores of quality of sleep before and after the intervention were significantly different in the intervention group (p=0.001). There was no significant difference between the two groups in terms of quality of sleep before (p=0.140) and immediately after the intervention (p=0.168). However, one month after, the difference between the two groups was significant (P<0.001). CONCLUSION Implementation of the continuous care model led to an improvement of quality of sleep in the menopausal women.

Jio-Bharti Airtel Data War and Strategy

2018 ◽  
pp. 171-179
Author(s):  
Anil Kumar ◽  
Mohan Balaji ◽  
Ujjwal Krishna ◽  
Mohit Yadav
Keyword(s):  
Quality Of Service ◽  
Regulatory Authority ◽  
The Other ◽  
Complete Understanding ◽  
Secondary Sources ◽  
The Difference

The chapter examines various viewpoints in regards to Reliance Jio and Bharti Airtel. This includes the entry of Jio in the telecom space along with the difference in the market Jio bought right after its introduction of the services for public. The focus area for the research has been the secondary sources. A complete understanding of both these companies has been taken into account for this study to pick up the points that both Jio and Bharti have been lacking upon and areas in which one has surpassed the other by attracting a new set of consumers into their family. The chapter as recorded by the facts presented through the Telecom Regulatory Authority of India Analytics Portal witnessed that Jio proved to have an upper hand in terms of the quality of service, which included data speed and tariffs. The other side of the story shows Bharti Airtel's lack of dominance in 4G experience delivery after Jio's entrance.

scholarly journals Morphometric Evaluation of Spermatogenic Cells and Seminiferous Tubules and Exploration of Luteinizing Hormone Beta Polypeptide in Testis of Datong Yak

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 66 ◽  
Author(s):  
Qudratullah Kalwar ◽  
Min Chu ◽  
Anum Ali Ahmad ◽  
Xuezhi Ding ◽  
Xiaoyun Wu ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Developmental Stages ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Sex Development ◽  
Significant Difference ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Morphometric Evaluation ◽  
Semi Quantitative Pcr

Histological examination of testes is essential for understanding infertility, sex development, and growth. Therefore, to understand the histomorphology of testes at different developmental stages, we performed hematoxylin and eosin staining of Yak testis. Our results revealed that the diameters of spermatogenic cells and their nuclei were significantly larger (p < 0.05) in the testis at six years compared to at six and 18 months. No significant difference was noted between 30 months and six years. The study was designed to compare the expression profile of LHB in Datong yak. The expression pattern of LHB was explored using quantitative PCR, semi-quantitative PCR, molecular bioinformatic, and Western blot analysis. Our observations indicated that expression of LHB was significantly higher (p < 0.05) in the testis of Datong yak. Western blotting indicated that the molecular mass of LHB protein was 16 kDa in yak. The protein encoded by yak LHB included conserved cysteine-knot domain regions. The high expression of LHB in testis indicated that LHB may be vital for the development of male gonads and the fertility of Datong yak.

scholarly journals A comparison of animated versus static images in an instructional multimedia presentation

2016 ◽  
Vol 40 (2) ◽  
pp. 201-205 ◽  
Author(s):  
C. J. Daly ◽  
J. M. Bulloch ◽  
M. Ma ◽  
D. Aidulis
Keyword(s):  
Student Satisfaction ◽  
Undergraduate Students ◽  
Three Dimensional ◽  
The Other ◽  
Multimedia Presentation ◽  
Still Images ◽  
Significant Difference ◽  
Essay Question ◽  
The Difference ◽  
Video Compositing

Sophisticated three-dimensional animation and video compositing software enables the creation of complex multimedia instructional movies. However, if the design of such presentations does not take account of cognitive load and multimedia theories, then their effectiveness as learning aids will be compromised. We investigated the use of animated images versus still images by creating two versions of a 4-min multimedia presentation on vascular neuroeffector transmission. One version comprised narration and animations, whereas the other animation comprised narration and still images. Fifty-four undergraduate students from level 3 pharmacology and physiology undergraduate degrees participated. Half of the students watched the full animation, and the other half watched the stills only. Students watched the presentation once and then answered a short essay question. Answers were coded and marked blind. The “animation” group scored 3.7 (SE: 0.4; out of 11), whereas the “stills” group scored 3.2 (SE: 0.5). The difference was not statistically significant. Further analysis of bonus marks, awarded for appropriate terminology use, detected a significant difference in one class (pharmacology) who scored 0.6 (SE: 0.2) versus 0.1 (SE: 0.1) for the animation versus stills group, respectively ( P = 0.04). However, when combined with the physiology group, the significance disappeared. Feedback from students was extremely positive and identified four main themes of interest. In conclusion, while increasing student satisfaction, we do not find strong evidence in favor of animated images over still images in this particular format. We also discuss the study design and offer suggestions for further investigations of this type.

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