A comparative study of influence of inflammation and collagen deposition in the cystic wall of infected and non-infected odontogenic keratocyst using picrosirius red and polarizing microscopy

2014 ◽  
Vol 5 (2) ◽  
pp. 83
Author(s):  
PriyaShirish Joshi ◽  
Madhuri Chougule ◽  
ManasiSandipak Kaijkar
Keyword(s):  
Comparative Study ◽  
Odontogenic Keratocyst ◽  
Collagen Deposition ◽  
Polarizing Microscopy ◽  
Cystic Wall ◽  
Picrosirius Red

scholarly journals Comparative study in swines' vocal cords healing after excision of fragment with CO2 laser with mitomycin and 5-fluorouracil postoperative topical application

2009 ◽  
Vol 24 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Eduardo Baptistella ◽  
Osvaldo Malafaia ◽  
Nicolau Gregori Czeczko ◽  
Jurandir Marcondes Ribas-Filho ◽  
Paulo Afonso Nunes Nassif ◽  
...  
Keyword(s):  
Comparative Study ◽  
Co2 Laser ◽  
Topical Application ◽  
Drug Application ◽  
Vocal Folds ◽  
Control Group ◽  
Collagen Deposition ◽  
Vocal Cords ◽  
The Mean ◽  
Picrosirius Red

PURPOSE: To evaluate the deposition of collagen fibers at pig's vocal folds after topical use of mitomycin or 5-fluorouracil, when partial exeresis of mucosa layer had been promoted by CO2 laser. METHODS: There were used 18 Larger white pigs which were anesthetized and submitted to mucosa fragment's exeresis, bilaterally, at its free border. The animals were divided into 3 groups, each one with 6 animals: control group, without topical drug application; mitomycin group; and 5-fluorouracil group. After 30 days, the animals were subjected to euthanasia, and samples of the vocal folds were collected and stained by picrosirius red technique with polarization for quantification of total collagen deposition. RESULTS: In control group, the mean rate of right vocal fold's collagen deposition at submucosa consisted in a 3428.66 micrometers area. There was found an area whose size had, in average, 2196.36 micrometers, in mitomycin group, and 2269.19 micrometers, in 5-fluorouracil group. CONCLUSION: Mitomycin and 5-fluorouracil had promoted beneficial change in vocal fold's cicatrization with less collagen deposition, but there was no significant statistically difference when they were compared between themselves.

scholarly journals Comparative Evaluation of Collagen Fibers in Odontogenic Keratocyst and Unicystic Ameloblastoma

2011 ◽  
Vol 2 (2) ◽  
pp. 125-127
Author(s):  
Vaidhehi N Nayak ◽  
Mandana Donoghue ◽  
M Selvamani
Keyword(s):  
Comparative Evaluation ◽  
Collagen Fibers ◽  
Odontogenic Keratocyst ◽  
Biological Behavior ◽  
Polarization Method ◽  
Polarizing Microscopy ◽  
Greenish Yellow ◽  
Unicystic Ameloblastoma ◽  
Red Dye ◽  
Picrosirius Red

ABSTRACT Background Odontogenic keratocyst (OKC) is an aggressive cyst with neoplastic behavior and unicystic ameloblastoma (UA) is a neoplasm with cyst like behavior. Both odontogenic keratocyst and unicystic ameloblastoma show biologic behavior unlike other lesions in their respective groups. In the present study, the biological behavior of these lesions were assessed by studying the collagen fibers in their walls using picrosirius red (PSR) polarization method. Methods Collagen fibers in 20 cases of OKC and 20 cases of UA were studied histologically by staining sections with picrosirius red dye and examining them by polarizing microscopy. Polarization colors of the collagen fibers of the lesions were recorded according to their width. Results While no differences were seen between the polarization colors of thin fibers (< 0.8 μ) in both odontogenic keratocyst and unicystic ameloblastoma, the polarization colors of thick fibers of odontogenic keratocyst were significantly more greenish-yellow as compared to the unicystic ameloblastoma which were predominantly yellowish-red. Conclusion These findings suggest that odontogenic keratocyst is a more aggressive lesion than unicystic ameloblastoma by means of identifying abnormally packed collagen fibers in odontogenic keratocyst but not in unicystic ameloblastoma. Thus, the nature of collagen fibers as studied by the picrosirius red polarization method may be useful as a diagnostic tool to differentiate between the two lesions.

scholarly journals Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

2017 ◽  
Vol 6 (2) ◽  
pp. 1518
Author(s):  
Parvez Ikram ◽  
Nadeem Jeddy
Keyword(s):  
Connective Tissue ◽  
Large Scale ◽  
Oral Submucous Fibrosis ◽  
Muscle Layer ◽  
Inclusion Criteria ◽  
Polarizing Microscopy ◽  
Potentially Malignant ◽  
Tissue Changes ◽  
Submucous Fibrosis ◽  
Picrosirius Red

<p><strong>Background:</strong> Oral submucous fibrosis (OSMF), a potentially malignant oral disorder has the highest rate of malignant transformation of about 7-13%. The connective tissue changes that occur in this disease are characteristic and are stained with special stains.</p><p><strong>Objective:</strong> The study was done to compare common and special stains under light microscopy and polarizing microscopy to evaluate the levels of fibrosis in oral submucous fibrosis and assess the type of collagen present in the stromal area.</p><p><strong>Materials and Methods:</strong> Fifty tissue blocks were selected from the archives and were prepared and stained with H&amp;E, Masson's trichrome, Van Gieson and Picrosirius red and studied under light microscope and polarizing microscope respectively.</p><p><strong>Results:</strong> H and E stained slides were useful in diagnosing the lesion but was not able to highlight the level of fibrosis. Masson's trichrome and Van Gieson stained slides showed the depth of the lesion which extended even to the deeper muscle layer. The type of collagen present was definitively seen by the birefringence in polarizing microscopic study. Interobserver variation was less and all the values regarding the effectiveness of the special stains in detecting the level of fibrosis were statistically significant.</p><p><strong>Conclusion:</strong> Special stains can be used routinely in laboratories to demonstrate connective tissue lesions especially in cases of OSMF. Depth of the lesion and the area of involvement help in treatment planning to be delivered. Large scale studies with more categories and inclusion criteria are required along with the special stains to assess the other alterations in OSMF.</p>

scholarly journals Cardiomyopathy in Offspring of Pregestational Diabetic Mouse Pregnancy

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Daniel Dowling ◽  
Niamh Corrigan ◽  
Stephen Horgan ◽  
Chris J. Watson ◽  
John Baugh ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Diabetic Mouse ◽  
Late Gestation ◽  
Collagen Deposition ◽  
Diabetic Mice ◽  
Intrauterine Environment ◽  
And Control ◽  
Picrosirius Red ◽  
Myocardial Collagen

Purpose. To investigate cardiomyopathy in offspring in a mouse model of pregestational type 1 diabetic pregnancy.Methods. Pregestational diabetes was induced with STZ administration in female C57BL6/J mice that were subsequently mated with healthy C57BL6/J males. Offspring were sacrificed at embryonic day 18.5 and 6-week adolescent and 12-week adult stages. The size and number of cardiomyocyte nuclei and also the extent of collagen deposition within the hearts of diabetic and control offspring were assessed following cardiac tissue staining with either haematoxylin and eosin or Picrosirius red and subsequently quantified using automated digital image analysis.Results. Offspring from diabetic mice at embryonic day 18.5 had a significantly higher number of cardiomyocyte nuclei present compared to controls. These nuclei were also significantly smaller than controls. Collagen deposition was shown to be significantly increased in the hearts of diabetic offspring at the same age. No significant differences were found between the groups at 6 and 12 weeks.Conclusions. Our results from offspring of type 1 diabetic mice show increased myocardial collagen deposition in late gestation and have increased myocardial nuclear counts (hyperplasia) as opposed to increased myocardial nuclear size (hypertrophy) in late gestation. These changes normalize postpartum after removal from the maternal intrauterine environment.

scholarly journals A Comparative Study of Proliferative Activity within Odontogenic Cyst Epithelium : Radicular Cyst and Odontogenic Keratocyst

1995 ◽  
Vol 49 (1) ◽  
pp. 157-165
Author(s):  
Qi Zhao ◽  
Tung Huai Hsu ◽  
Eiko Nakanishi ◽  
Chihiro Iuchi ◽  
Michihiro Takubo ◽  
...  
Keyword(s):  
Comparative Study ◽  
Proliferative Activity ◽  
Odontogenic Cyst ◽  
Odontogenic Keratocyst ◽  
Radicular Cyst
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