scholarly journals Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

2016 ◽  
Vol 11 (12) ◽  
pp. 1962 ◽  
Author(s):  
Yong-zhi Ji ◽  
Long Geng ◽  
Hong-bo Zhou ◽  
Hua-chen Wei ◽  
Hong-duo Chen
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Chinese Herbal ◽  
Anterior Pituitary Cells ◽  
Yougui Pill ◽  
Induced Apoptosis

Estrogens exert a rapid apoptotic action in anterior pituitary cells

2009 ◽  
Vol 296 (4) ◽  
pp. E664-E671 ◽  
Author(s):  
S. Zárate ◽  
G. Jaita ◽  
V. Zaldivar ◽  
D. B. Radl ◽  
G. Eijo ◽  
...  
Keyword(s):  
Anterior Pituitary ◽  
Primary Cultures ◽  
Ovariectomized Rats ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Steroid Signaling ◽  
A Cell ◽  
17Β Estradiol ◽  
Induced Apoptosis ◽  
Classical Mechanism

It is now accepted that estrogens not only stimulate lactotrope proliferation but also sensitize anterior pituitary cells to proapoptotic stimuli. In addition to their classical mechanism of action through binding to intracellular estrogen receptors (ERs), there is increasing evidence that estrogens exert rapid actions mediated by cell membrane-localized ERs (mERs). In the present study, we examined the involvement of membrane-initiated steroid signaling in the proapoptotic action of estradiol in primary cultures of anterior pituitary cells from ovariectomized rats by using estren, a synthetic estrogen with no effect on classical transcription and a cell-impermeable 17β-estradiol conjugate (E2-BSA). Both compounds induced cell death of anterior pituitary cells after 60 min of incubation as assessed by flow cytometry and the [3-(4,5-dimethylthiazol-2-yl)]-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Estren, E2, and E2-BSA induced apoptosis of lactotropes and somatotropes as evaluated by the deoxynucleotidyltransferase-mediated dUTP nick end-labeling assay and immunodetection of prolactin (PRL) and growth hormone (GH). The proapoptotic effect of E2-BSA was abrogated by ICI-182,780, an antagonist of ERs. The expression of membrane-associated ERα was observed in PRL- and GH-bearing cells. Our results indicate that estradiol is able to exert a rapid apoptotic action in anterior pituitary cells, especially lactotropes and somatotropes, by a mechanism triggered by mERs. This mechanism could be involved in anterior pituitary cell turnover.

Nitric oxide protects anterior pituitary cells from cadmium-induced apoptosis

2004 ◽  
Vol 37 (9) ◽  
pp. 1463-1471 ◽  
Author(s):  
Ariel H.B. Poliandri ◽  
Miguel O. Velardez ◽  
Jimena P. Cabilla ◽  
Cristian C.A. Bodo ◽  
Leticia I. Machiavelli ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Induced Apoptosis

Mechanisms of chromium (VI)-induced apoptosis in anterior pituitary cells

Toxicology ◽  
2008 ◽  
Vol 249 (2-3) ◽  
pp. 109-115 ◽  
Author(s):  
Fernanda A. Quinteros ◽  
Leticia I. Machiavelli ◽  
Eliana A. Miler ◽  
Jimena P. Cabilla ◽  
Beatriz H. Duvilanski
Keyword(s):  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Chromium Vi ◽  
Anterior Pituitary Cells ◽  
Induced Apoptosis

scholarly journals Estrogens Up-Regulate the Fas/FasL Apoptotic Pathway in Lactotropes

Endocrinology ◽  
2005 ◽  
Vol 146 (11) ◽  
pp. 4737-4744 ◽  
Author(s):  
G. Jaita ◽  
M. Candolfi ◽  
V. Zaldivar ◽  
S. Zárate ◽  
L. Ferrari ◽  
...  
Keyword(s):  
Anterior Pituitary ◽  
Cell Types ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Pituitary Cells ◽  
Cell Renewal ◽  
Apoptotic Pathway ◽  
Anterior Pituitary Cells ◽  
Ovx Rats ◽  
17Β Estradiol ◽  
Induced Apoptosis

The Fas/FasL system provides the major apoptotic mechanism for many cell types, participating in cell turnover in hormone-dependent tissues. In the present study, we localized both Fas and FasL in anterior pituitary cells, mainly in lactotropes and somatotropes. The percentage of anterior pituitary cells showing immunoreactivity for Fas or FasL was higher in cells from rats killed in proestrus than in diestrus. Also, the proportion of pituitary cells from ovariectomized (OVX) rats expressing Fas or FasL increased in the presence of 17β-estradiol (10−9m). This steroid increased the percentage of lactotropes with immunoreactivity for Fas or FasL and the percentage of somatotropes expressing Fas. Activation of Fas by an agonist anti-Fas antibody (Mab-Fas) decreased the vi-ability—3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT assay)—of anterior pituitary cells from OVX rats cultured in the presence of 17β-estradiol. Also, membrane-bound FasL decreased cell viability—[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay (MTS assay)—only when anterior pituitary cells from OVX rats were incubated with 17β-estradiol. Moreover, FasL increased the percentage of hypodiploid anterior pituitary cells (flow cytometry). Mab-Fas increased the percentage of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL)-positive pituitary cells and lactotropes from OVX rats only when cells were incubated in the presence of 17β-estradiol. Also, Mab-Fas triggered apoptosis of anterior pituitary cells from rats killed at proestrus but not at diestrus. Our results show that 17β-estradiol up-regulates the expression of the Fas/FasL system in anterior pituitary cells and increases Fas-induced apoptosis in lactotropes, suggesting that Fas-induced apoptosis could be involved in the pituitary cell renewal during the estrous cycle.

The effect of the chinese herbal medicine Dai-Kenchu-To on intestinal blood flow

2001 ◽  
Vol 120 (5) ◽  
pp. A248-A248
Author(s):  
N KAWASAKI ◽  
K NARIAI ◽  
M NAKAO ◽  
K NAKADA ◽  
N HANYUU ◽  
...  
Keyword(s):  
Blood Flow ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Intestinal Blood Flow ◽  
Chinese Herbal

Treatment of atopic dermatitis with Chinese herbal medicine. Therapeutic effects of Hochuekkitou.

1989 ◽  
Vol 51 (5) ◽  
pp. 1003-1013 ◽  
Author(s):  
Hiromi KOBAYASHI ◽  
Masamitsu ISHII ◽  
Tsukasa TANII ◽  
Takeshi KOHNO ◽  
Toshio HAMADA
Keyword(s):  
Atopic Dermatitis ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Therapeutic Effects ◽  
Chinese Herbal
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