Immunohistochemical expression of cyclin D1 in invasive breast carcinoma and its correlation with clinicopathological parameters

INTRODUCTION: Triple negative breast carcinoma (TNBC) molecular subtype and its variant; basal-like triple negative (BLTN) carcinoma is an established poor prognostic indicator. The aim of this study is to analyse the survivin expression in TNBC and BLTN, and relating the results with clinicopathological parameters. MATERIALS AND METHODS: We conducted a cross sectional study using 94 archived formalin-fixed paraffin embedded tissue blocks of invasive breast carcinoma, no special type (NST). Estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth receptor 2 (HER2) were used as surrogate markers to classify the cases into molecular subtypes. Samples with triple negative phenotype (ER, PR and HER2 negative) were stained with CK 5/6 to identify the BLTN subtype. All the samples were also immunostained for survivin. RESULT: Out of 94 cases, 41.5% (39 cases) were TNBC. Among the TNBC cases, only 41.0% (16 cases) were BLTN subtype when they found to be positive for CK 5/6. Among 94 cases of invasive breast carcinoma, 28.7% (27 cases) were survivin positive with (53.8%) 21 cases were TNBC and (11%) 6 cases were non-TNBC (p< 0.001). Among 16 cases of BLTN subtype, only 8 cases were survivin positive (p = 0.752). Survivin expression was also statistically significant with tubule formation (p=0.029), nuclear pleomorphism (p=0.008), tumour grade (p=0.010), ER status (p< 0.001) and PR status (p=0.001). CONCLUSION: Survivin expression was statistically significant in invasive breast carcinoma. Even though the expression was significantly high in TNBC, it is not related to whether it is a basal-like or non-basal-like variant.

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Cyclin-d1-gene amplification and expression in breast carcinoma: Relation with clinicopathologic characteristics and with retinoblastoma gene product, p53 and p21waf1 immunohistochemical expression

International Journal of Cancer ◽

10.1002/(sici)1097-0215(19970422)74:2<171::aid-ijc5>3.0.co;2-w ◽

1997 ◽

Vol 74 (2) ◽

pp. 171-174 ◽

Cited By ~ 50

Author(s):

Mattia Barbareschi ◽

Paola Pelosio ◽

Orazio Caffo ◽

Fiamma Buttitta ◽

Silvia Pellegrini ◽

...

Keyword(s):

Breast Carcinoma ◽

Cyclin D1 ◽

Gene Product ◽

Gene Amplification ◽

Retinoblastoma Gene ◽

Immunohistochemical Expression ◽

Clinicopathologic Characteristics ◽

Retinoblastoma Gene Product

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The Immunohistochemical Expression of the Oestrogen Receptor (ER), HER-2/NEU and Cytokeratin 8/18 and 5/6 in Invasive Breast Carcinoma

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2012/4086.254 ◽

2012 ◽

Author(s):

Bharti Rattan

Keyword(s):

Breast Carcinoma ◽

Oestrogen Receptor ◽

Invasive Breast Carcinoma ◽

Immunohistochemical Expression ◽

Her 2

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Detection of pAkt protein in imprint cytology of invasive breast cancer: Correlation with HER2/neu, hormone receptors, and other clinicopathological variables

CytoJournal ◽

10.4103/1742-6413.153965 ◽

2015 ◽

Vol 12 ◽

pp. 6 ◽

Cited By ~ 1

Author(s):

Olympia Vasou ◽

Lazaros Skagias ◽

Margariti Anastasia ◽

Athanasiadou Paulina ◽

Efstratios Patsouris ◽

...

Keyword(s):

Breast Cancer ◽

Protein Kinase ◽

Breast Carcinoma ◽

Invasive Breast Carcinoma ◽

Prognostic Models ◽

Clinicopathological Parameters ◽

Axillary Lymph Nodes ◽

Positive Staining ◽

Axillary Lymph ◽

Cytoplasmic Staining

Purpose: Akt is a serine/threonine protein kinase and has emerged as a crucial regulator of widely divergent cellular processes, including apoptosis, proliferation, differentiation, and metabolism. Activation of Akt/protein kinase B has been positively associated with human epidermal growth-factor receptor 2 (HER2)/neu overexpression in breast carcinoma and a worse outcome among endocrine treated patients. The Akt signaling pathway currently attracts considerable attention as a new target for effective therapeutic strategies. We therefore investigated the relationship between activation of Akt and clinicopathologic variables including hormone receptor and HER2/neu status. Methods: Archival tumor tissues from 100 patients with invasive breast carcinoma were analyzed by immunocytochemistry. This study describes the results of immunocytochemical pAkt expression in breast carcinoma imprints, prepared from cut surfaces of freshly removed tumors. Both nuclear and cytoplasmic expressions were evaluated for pAkt. Results: Nuclear and cytoplasmic positive scores of 72% (72/100) and 42% (42/100), respectively, were found. Coexistence of nuclear and cytoplasmic staining was observed in 32 cases (32/100). Nuclear positive staining correlated with HER2/neu overexpression (P = 0.043) and was significantly associated with positive involvement of axillary lymph nodes (P = 0.013). No correlation was found between cytoplasmic pAkt rate and clinicopathological parameters, estrogen receptor, progesterone receptor or HER2/neu expression. Conclusions: pAkt expression can be evaluated in cytological material and may add valuable information to current prognostic models for breast cancer. pAkt overexpression appears to be linked with potentially aggressive tumor phenotype in invasive breast carcinoma.

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