Variability in the rumen degradability and postruminal digestion of the dry matter, nitrogen and amino acids of canola meal

1991 ◽  
Vol 71 (3) ◽  
pp. 739-754 ◽  
Author(s):  
E. M. Kendall ◽  
J. R. Ingalls ◽  
R. J. Boila
Keyword(s):  
Amino Acids ◽  
Key Words ◽  
Soybean Meal ◽  
Dry Matter ◽  
Essential Amino Acids ◽  
Canola Meal ◽  
Ruminal Fermentation ◽  
Processing Plants

The rumen degradability and postruminal digestion of rumen escape dry matter (DM), nitrogen (N) and metabolically essential amino acids (EAA) for canola meal (CM) from five different processing plants (samples 1–5) and one sample of soybean meal (SBM) were compared. Rumen degradability was estimated by incubating sample-filled small nylon bags in situ in the rumen of steers for 0, 4, 8, 12, 16 and 30 h. Postruminal digestion was estimated using a sequence of ruminal in situ incubations for 0, 4, 8, 12 and 16 h, in vitro incubation in an acid-pepsin solution and a mobile nylon bag technique distal to the abomasum. The rumen escape of nutrients for CM1 was consistently lower compared to other CM samples, while CM4 resulted in the highest escape of nutrients at 30 h. An estimate of maximum degradability of EAA measured at 30 h differed among CM samples, with the rumen escape of EAA being CM4 > CM2 = CM5 ≥ CM1 = CM3 > SBM. The DM, N and EAA content of mobile bag residues was relatively uniform among CM samples, particularly after 12 and 16 h of ruminal fermentation, but was higher for CM than for SBM, probably due to a low digestibility of CM hulls. The rumen degradability of CM from different processing plants differed and as a consequence the postruminal digestion of nutrients in CM differed. Key words: Canola meal, rumen degradability, postruminal digestion, dry matter, nitrogen, amino acids

In situ rumen digestion and escape of dry matter, nitrogen and amino acids in canola meal

1992 ◽  
Vol 72 (4) ◽  
pp. 891-901 ◽  
Author(s):  
R. J. Boila ◽  
J. R. Ingalls
Keyword(s):  
Amino Acids ◽  
Key Words ◽  
Dry Matter ◽  
Canola Meal ◽  
Poor Indicator ◽  
Protein Amino Acids

Three rumen-cannulated steers were used in three separate trials wherein canola meal (CM) was incubated in situ for 0.1, 4, 8, 12, 16 and 36 h in Dacron bags. Disappearance parameters were estimated for dry matter (DM), nitrogen (N) and individual amino acids (AAs). Effective degradabilities (EDs) were estimated at rumen particulate outflow rates of 0.08, 0.05 and 0.02 h−1. An ED for DM that was lower than that of most AAs indicated there was a limit to its degradability. In contrast, the ED of N was higher than that of 11 of 17 AAs; the disappearance parameters for N were a poor indicator of those estimated for individual AAs. The concentrations of AAs in escape CM estimated at the specified rumen outflow times, compared with non-incubated CM, were higher for all AAs except cystine, proline and glutamate, whose concentrations were lower, and histidine and arginine, whose concentrations did not differ. Compared with non-incubated CM, in escape CM there was a 33% reduction in concentration of glutamate, a 28% enrichment of tyrosine, a lower enrichment, ranging from + 14 to + 23%, for valine, isoleucine, threonine, phenylalanine, serine, aspartate and alanine, and little change in concentration for other AAs. Although the concentrations of AAs in rumen escape CM differed from that in non-incubated CM, those concentrations in escape CM were relatively uniform among outflow rates of 0.08, 0.05 and 0.02 h−1. Key words: Canola meal, in situ digestion, rumen degradability, rumen escape, protein, amino acids

Impact of a tannin extract on digestibility, ruminal fermentation and duodenal flow of amino acids in steers fed maize silage and concentrate containing soybean meal or canola meal as protein source

2015 ◽  
Vol 153 (5) ◽  
pp. 943-953 ◽  
Author(s):  
S. C. ÁVILA ◽  
G. V. KOZLOSKI ◽  
T. ORLANDI ◽  
M. P. MEZZOMO ◽  
S. STEFANELLO
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Rumen Fluid ◽  
Protein Source ◽  
Canola Meal ◽  
Ruminal Fermentation ◽  
Tannin Extract ◽  
Maize Silage ◽  
Microbial N

SUMMARYFour Holstein steers (297 ± 56 kg of body weight (BW)) fitted with duodenal cannula and rumen catheter, were housed in metabolism cages and used in a 4 × 4 Latin Square trial to evaluate the effect of both protein source andAcacia mearnsiitannin extract on digestibility, ruminal fermentation, rumen microbial protein synthesis, N utilization and on duodenal flow of individual amino acids. The diet was offered at restricted amount of 25 g of dry matter (DM)/kg BW and consisted of maize silage plus concentrate, in a proportion of 0·7:0·3 (DM basis) respectively. Concentrate was formulated with either soybean meal or canola meal as protein source, with or without 50 g/kg ofA. mearnsiitannin extract (i.e. 15 g/kg of total dietary DM). There was no effect of protein source on most variables. The apparent and true organic matter (OM) digestibilities, as well as neutral detergent fibre (NDF) digestibility were negatively affected by tannin extract inclusion without, however, affecting digestible OM intake. The amount of nitrogen (N) excreted in faeces increased whereas the urinary N excretion decreased in tannin extract treatments. No interaction time × treatment was detected for any rumen variable and no treatment effect was observed for rumen fluid pH and reducing sugars concentration. Rumen fluid concentration of ammonia N was lower for the canola meal plus tannin extract treatment. Rumen concentration ofα-amino compounds was not affected by tannin extract but was higher when canola meal was the protein source. The duodenal flow of OM, total N,α-amino N and non-ammonia non-microbial N increased with tannin extract inclusion, whereas the duodenal flow of microbial N was not affected by treatment. For both protein sources, the amount of most individual amino acids flowing to the duodenum increased due to tannin extract addition. In conclusion, the dietary inclusion of 15 g/kg DM of tannin extract fromA. mearnsiiimproved the amino acid supply independently of whether the protein source was canola meal or soybean meal, without affecting the amino acid profile, to steers fed maize silage plus concentrate, with a minor but significant impact on OM digestibility.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

Protein composition and ruminal amino acid degradability of linseed meal

2000 ◽  
Vol 80 (4) ◽  
pp. 745-747
Author(s):  
A. F. Mustafa ◽  
D. A. Christensen ◽  
J. J. McKinnon
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Key Words ◽  
Acid Composition ◽  
Protein Composition ◽  
Essential Amino Acids ◽  
Canola Meal ◽  
Linseed Meal ◽  
Ruminal Degradability

A study was conducted to determine amino acid composition and ruminal degradability of linseed meal (LSM) relative to canola meal (CM). Linseed meal had higher (P < 0.05) arginine, isoleucine, and phenylalanine and less (P < 0.05) lysine than CM. Except for histidine, methionine, and threonine, ruminal escape values of essential amino acids were higher (P < 0.05) for LSM than CM. Key words: Linseed meal, amino acids, ruminal degradability

scholarly journals In vitro ruminal fermentation kinetics of diets with crambe cake protein replacing soybean meal protein by gas production technique

2021 ◽  
Vol 42 (6) ◽  
pp. 3399-3414
Author(s):  
Angela Rocio Poveda-Parra ◽  
Odimári Pricila Prado-Calixto ◽  
Elzânia Sales Pereira ◽  
Fernando Luiz Massaro Junior ◽  
Larissa Nóbrega de Carvalho ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Dry Matter ◽  
Gas Production ◽  
Corn Silage ◽  
Ruminal Fermentation ◽  
Production Technique ◽  
Meal Protein ◽  
Feedlot Lambs ◽  
Gas Volume

The objective of this study was to evaluate ingredients and diets containing increasing levels of crambe cake protein replacing soybean meal protein, with in vitro ruminal fermentation parameters using a gas production technique. Diets were formulated for feedlot lambs and contained different levels of crambe cake protein (0, 250, 500, 750, and 1000 g kg-1) replacing soybean meal protein. Corn silage was used as roughage. Carbohydrate digestion rates were estimated using the in vitro gas production technique and the cumulative gas production kinetics were analyzed using the bicompartmental logistic model. The parameters values of ruminal degradation kinetics were generated using the R statistical program with the Gauss-Newton algorithm and then subjected to analysis of variance and regression (when necessary) according to a completely randomized experimental design with five treatments and five replications. Upon carbohydrate fractionation of ingredients and experimental diets, it was observed that corn grain and corn silage presented the highest levels of total carbohydrates (TC), with values of 128.3 and 464.8 g kg-1 dry matter (DM) in fraction B2, respectively. Lower TC content was found for soybean meal and crambe cake (CC). There was a predominance of fractions A + B1 in the ingredients and experimental diets. The B2 fraction decreased in the diets with the inclusion of the CC protein, and CC presented the highest C fraction. Protein fractionation (g kg-1 DM and g kg-1 crude protein - CP), the ingredients and diets showed a higher proportion of fractions A and B1 + B2. In in vitro degradation, the diet without CC (0 g kg-1 DM) showed the highest final cumulative gas production (365.04 mL g-1 of incubated DM), while the CC presented the lowest volume (166.68 mL g-1 of incubated DM). The gas volume of non-fibrous carbohydrate fermentation and fibrous carbohydrate degradation rate exhibited a quadratic effect according increasing levels of CC (Pmax = 265.8 g kg-1 DM and Pmin = 376.3 g kg-1 DM, respectively). The lag time and final gas volume showed a decreasing linear effect with increasing levels of CC protein. The degradation rate of non-fibrous carbohydrates and the final volume of fibrous carbohydrates did not differ. Replacing soybean meal protein with CC protein at the level of 250 g kg-1 of dry matter in diets formulated for feedlot lambs leads to good profiles of ruminal fermentation kinetics with respect to the degradation of fibrous and non-fibrous carbohydrates.

Comparison of lignosulfonate-treated canola meal and soybean meal as rumen undegradable protein supplements for lambs

1995 ◽  
Vol 75 (3) ◽  
pp. 371-377 ◽  
Author(s):  
K. Stanford ◽  
T. A. McAllister ◽  
Z. Xu ◽  
K.-J. Cheng ◽  
M. Pickard
Keyword(s):  
Soybean Meal ◽  
Average Daily Gain ◽  
Canola Meal ◽  
Feed Conversion ◽  
Protein Supplements ◽  
Rumen Undegradable Protein ◽  
Daily Gain ◽  
Growth Experiments

An experiment was undertaken to determine the effect of lignosulfonate (LSO3) treatment on the rumen-undegradable protein (RUDP) value of canola meal (CM) and soybean meal (SM). Canola meal and SM were treated with 7% LSO3 and heated (95 °C) for 1 h. Treated and untreated meals were compared in vitro, in situ and in digestibility and growth experiments using Romanov × Suffolk and Romanov × Dorset lambs. Lambs were fed four barley-based diets [13.5% crude protein (CP)] with one of CM, SM, LSO3-treated CM (LSO3-CM) or LSO3-treated SM (LSO3-SM) as protein supplements. Lignosulfonate treatment increased acid detergent insoluble nitrogen (ADIN) five-fold in SM and threefold in CM. After 6 h incubation with ruminal fluid, ammonia production from LSO3-treated meals was one-half that from untreated meals. Lignosulfonate treatment reduced (P < 0.01) in situ rates of dry matter (DM) and protein disappearance and the soluble DM and protein fractions for both SM and CM, but not the potentially digestible fractions. No differences in feed conversion or average daily gain (P > 0.05) were found among the treatments. Although LSO3 treatment did not improve the ADG of lambs fed barley-based diets, it increased the RUDP value of SM and CM without adverse effects on digestibility. Key words: Lignosulfonate, canola meal, soybean meal, lamb

In situ amino acid disappearance from regular, low and high fiber canola meal

1997 ◽  
Vol 77 (3) ◽  
pp. 533-535 ◽  
Author(s):  
A. F. Mustafa ◽  
J. J. McKinnon ◽  
D. A. Christensen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Key Words ◽  
Canola Meal ◽  
High Fiber

In situ rumen disappearance of low and high fiber canola meal relative to regular canola meal was determined following 12 h of rumen incubation. In situ amino acid disappearance was similar in low and high fiber and regular canola meal except for threonine and glutamate which was lower (P < 0.05) in high fiber canola meal. It was concluded that ruminal disappearance of amino acids was similar across the three meal types. Key words: Tail-end dehulling, canola meal, high fiber canola meal, low fiber canola meal

EFFECTS OF pH AND CANOLA MEAL ON TRACE MINERAL SOLUBILITY IN VITRO

1987 ◽  
Vol 67 (2) ◽  
pp. 587-590
Author(s):  
M. O. KEITH ◽  
J. M. BELL
Keyword(s):  
Key Words ◽  
Soybean Meal ◽  
Trace Minerals ◽  
Canola Meal ◽  
Trace Mineral ◽  
Mineral Solubility ◽  
Effects Of Ph

An in vitro procedure was used to examine the effects of pH and canola meal (CM) on the solubility of copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn). Solubility of Cu, Mn and Zn was reduced (P < 0.001) by increasing pH. Mn solubility was reduced (P < 0.001) with increasing CM concentration. CM had a greater effect than soybean meal (P < 0.001) in reducing the solubility of all four trace minerals. Key words: Canola meal, trace minerals, availability

scholarly journals Slow-Release Urea as a Sustainable Alternative to Soybean Meal in Ruminant Nutrition

2021 ◽  
Vol 13 (5) ◽  
pp. 2464
Author(s):  
Saheed A. Salami ◽  
Maria Devant ◽  
Juha Apajalahti ◽  
Vaughn Holder ◽  
Sini Salomaa ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Dry Matter ◽  
Vegetable Protein ◽  
Slow Release ◽  
Protein Sources ◽  
Slow Release Urea ◽  
In Vitro Rumen Fermentation

Three experiments were conducted to evaluate the feasibility of using a commercial slow-release urea product (SRU; Optigen®, Alltech Inc., Nicholasville, KY, USA) as a partial replacement for vegetable protein sources in cattle diets. The first experiment was an in vitro rumen fermentation that evaluated the effect of replacing soybean meal (SBM) nitrogen with nitrogen from either SRU or free urea in diets varying in forage:concentrate ratios. The second experiment examined the effect of replacing SBM with SRU on in situ dry matter and nitrogen degradability in the rumen. In the third experiment, a feeding trial was conducted to evaluate the effect of replacing SBM (0% as-fed SRU) with 1% or 3% as-fed SRU on feed carbon footprint (CFP; total greenhouse gas emissions associated with the life cycle of feed raw materials) and the toxicity potential of SRU in growing beef cattle. Results showed that replacing SBM with SRU up to 1.3% did not negatively affect in vitro rumen fermentation parameters. Supplementing SRU favourably decreased ruminal accumulation of ammonia and lactic acid when compared to free urea. There was no significant effect on effective rumen degradability of dry matter and nitrogen when one-third of SBM was replaced by SRU in the in situ study. Compared with the 0% SRU diet, feed CFP decreased by 18% and 54% in 1% SRU and 3% SRU diets, respectively. Additionally, feeding up to 3% SRU diet to beef cattle did not affect health and intake, and blood hematological and biochemical indices were within the physiological range for healthy bulls, suggesting no indication of ammonia toxicity. Overall, these results indicate that SRU can be used as a sustainable alternative to partially replace vegetable protein sources in ruminant diets without compromising rumen function and health of ruminants.

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