In Vitro Cultivation of Plasmodium Falciparum at High Parasitemia *

1974 ◽  
Vol 23 (6) ◽  
pp. 1015-1018 ◽  
Author(s):  
Wasim A. Siddiqui ◽  
Suzanne Richmond-Crum ◽  
Jerome V. Schnell
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Cultivation ◽  
High Parasitemia

Growth, Drug Susceptibility, and Gene Expression Profiling of Plasmodium falciparum Cultured in Medium Supplemented with Human Serum

2007 ◽  
Vol 12 (8) ◽  
pp. 1109-1114 ◽  
Author(s):  
Kshipra Singh ◽  
Ameeta Agarwal ◽  
Shabana I. Khan ◽  
Larry A. Walker ◽  
Babu L. Tekwani
Keyword(s):  
Gene Expression ◽  
Plasmodium Falciparum ◽  
Human Serum ◽  
Global Gene Expression ◽  
Drug Susceptibility ◽  
Growth Cycle ◽  
In Vitro Cultivation ◽  
Human Malaria Parasite ◽  
In Vitro Drug Susceptibility

In vitro cultivation of Plasmodium falciparum has been extremely useful in understanding the biology of the human malaria parasite as well as research on the discovery of new antimalarial drugs and vaccines. A chemically defined serum-free medium supplemented with lipid-rich bovine serum albumin (AlbuMAX I) offers the following advantages over human serum-supplemented media for the in vitro culture of P. falciparum: 1) improved growth profile, with more than a 2-fold higher yield of the parasites at any stage of the growth cycle; 2) suitability for in vitro antimalarial screening, as the parasites grown in AlbuMAX and human serum-supplemented media show similar sensitivity to standard and novel antimalarials as well as natural product extracts in the in vitro drug susceptibility assays; and 3) DNA microarray analysis comparing the global gene expression profile of sorbitol-synchronized P. falciparum trophozoites grown in the 2 different media, indicating minimal differences. ( Journal of Biomolecular Screening 2007:1109-1114)

In Vitro Cultivation of Plasmodium falciparum *

1970 ◽  
Vol 19 (4) ◽  
pp. 586-591 ◽  
Author(s):  
Wasim A. Siddiqui ◽  
Quentin M. Geiman ◽  
Jerome V. Schnell
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Cultivation

scholarly journals Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ann-Kathrin Tilly ◽  
Jenny Thiede ◽  
Nahla Metwally ◽  
Pedro Lubiana ◽  
Anna Bachmann ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Localization ◽  
In Vitro Cultivation ◽  
Transcriptome Profile

scholarly journals Impact of sickle cell trait hemoglobin on the intraerythrocytic transcriptional program of Plasmodium falciparum

2021 ◽  
Author(s):  
Joseph W Saelens ◽  
Jens E.V. Petersen ◽  
Elizabeth Freedman ◽  
Robert C Moseley ◽  
Drissa Konate ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Differential Expression ◽  
Molecular Mechanisms ◽  
Sickle Cell Trait ◽  
Differential Expression Analysis ◽  
Protein Export ◽  
In Vitro Cultivation ◽  
Transcriptional Program ◽  
Transcriptional Responses

Sickle-trait hemoglobin (HbAS) confers near-complete protection from severe, life-threatening falciparum malaria in African children. Despite this clear protection, the molecular mechanisms by which HbAS confers these protective phenotypes remain incompletely understood. As a forward genetic screen for aberrant parasite transcriptional responses associated with parasite neutralization in HbAS red blood cells (RBCs), we performed comparative transcriptomic analyses of Plasmodium falciparum in normal (HbAA) and HbAS erythrocytes during both in vitro cultivation of reference parasite strains and naturally-occurring P. falciparum infections in Malian children with HbAA or HbAS. During in vitro cultivation, parasites matured normally in HbAS RBCs, and the temporal expression was largely unperturbed of the highly ordered transcriptional program that underlies the parasites maturation throughout the intraerythrocytic development cycle (IDC). However, differential expression analysis identified hundreds of transcripts aberrantly expressed in HbAS, largely occurring late in the IDC. Surprisingly, transcripts encoding members of the Maurers clefts were overexpressed in HbAS despite impaired parasite protein export in these RBCs, while parasites in HbAS RBCs underexpressed transcripts associated with the endoplasmic reticulum and those encoding serine repeat antigen proteases that promote parasite egress. Analyses of P. falciparum transcriptomes from 32 children with uncomplicated malaria identified stage-specific differential expression: among infections composed of ring-stage parasites, only cyclophilin 19B was underexpressed in children with HbAS, while trophozoite-stage infections identified a range of differentially-expressed transcripts, including downregulation in HbAS of several transcripts associated with severe malaria in collateral studies. Collectively, our comparative transcriptomic screen in vitro and in vivo indicates that P. falciparum adapts to HbAS by altering its protein chaperone and folding machinery, oxidative stress response, and protein export machinery. Because HbAS consistently protects from severe P. falciparum, modulation of these responses may offer avenues by which to neutralize P. falciparum parasites.

Sensitivity of Plasmodium falciparum field-isolates from Tanzania to chloroquine, mefloquine and pyrimethamine during in vitro cultivation

Acta Tropica ◽  
1993 ◽  
Vol 52 (4) ◽  
pp. 313-316 ◽  
Author(s):  
W. Huber ◽  
N. Hurt ◽  
H. Mshinda ◽  
C. Jaquet ◽  
J.C. Koella ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Cultivation ◽  
Field Isolates
Sign in / Sign up

Export Citation Format

Share Document