scholarly journals Associations of transcription factor 7-Like 2 (TCF7L2) gene polymorphism in patients of type 2 diabetes mellitus from Khyber Pakhtunkhwa population of Pakistan

2021 ◽  
Vol 21 (1) ◽  
pp. 15-22
Author(s):  
Taha Hameed ◽  
Zahid Khan ◽  
Muhammad Imran ◽  
Saif Ali ◽  
Abdullah Abdo Albegali ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Transcription Factor ◽  
Genetic Association ◽  
Amplification Refractory Mutation System ◽  
Tcf7l2 Gene ◽  
Khyber Pakhtunkhwa ◽  
Arms Pcr

Background: Type 2 diabetes mellitus (T2DM) is the most prevalent component of metabolic syndrome. Environmental factors and various complex genes like transcription factor 7-like 2 (TCF7L2) gene have involved in the disease development. Objective: To determine TCF7L2 genetic association (rs7903146C/T and rs12255372G/T) in T2DM patients of Khyber Pakhtunkhwa population of Pakistan. Subjects and methods: This study comprised of 176 subjects including 118 T2DM patients and 58 healthy controls. Genomic DNA was extracted and genotype of common variants (rs7903146 C/T and rs12255372 G/T) was carried out by amplification-refractory mutation system (ARMS)-PCR of sequence specific oligonucleotides. Results: The distribution of genotype of TCF7L2 SNPs (rs7903146 C/T and rs12255372 G/T) was significantly associated with T2DM as compared to the controls (p <0.0001). The genetic models of the rs7903146 (C/T) and rs12255372 (G/T) SNPs were significantly associated between cases and controls (p <0.0001). On the other hand, the significant association was observed between the two SNPs and different biochemical parameters like serum fasting glucose, lipid profile, creatinine and blood HbA1c levels (p <0.05). Conclusion: It is concluded that the SNPs of the TCF7L2 gene are significantly associated with T2DM disease susceptibil- ity in the population of Khyber Pakhtunkhwa of Pakistan. Keywords: T2DM; TCF7L2; Genetic association, ARMS-PCR, Single nucleotide Polymorphism (SNPs), Khyber Pakh- tunkhwa.

scholarly journals Polymorphism of Transcription Factor 7-Like 2 Gene and HOMA-β Level of Individuals With and Without Type 2 Diabetes Mellitus Family History

2016 ◽  
Vol 19 (2) ◽  
pp. 176
Author(s):  
Waode Astria Sahrani ◽  
Indwiani Astuti ◽  
Ahmad Hamim Sadewa
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Transcription Factor ◽  
Insulin Secretion ◽  
Family History ◽  
Model Assessment ◽  
Tcf7l2 Gene ◽  
Type 2 Dm

Family history has considered as a risk factor of type 2 diabetes. Transcription factor-7 like 2 (TCF7L2) hasrole to regulates insulin secretion and blood glucose homeostasis. The aim of current study was to determine thers7903146 polymorphism of TCF7L2 gene and homeostatic model assessment-β (HOMA-β) level on individual withand without type 2 Diabetes Mellitus (DM) family history. This work is a case-control study. Thirty six subjectswith type 2 DM family history and 36 subjects without type 2 DM family history were recruited. HOMA-βmeasure to analyze the insulin secretion. Polymorphisms of TCF7L2 gene was analyzed by using PCR-RFLPmethod. Statistical analysis was performed by using T-test, Mann-Whitney and Chi-square with signifi cancelevel 0.05. The frequency of the T allele of the cases were 4.2% and the controls were 2.8% (p=0.500). The oddratio was 0.649 (CI;95%:0.106-4.055). The HOMA-β levels of the cases were signifi cant low (132.56±62.48)compared with the controls (266.09±1.68) with p=0.000. The subjects with type 2 DM family history have asimilar frequency of having T alleles and CT/TT genotypes. The subjects with type 2 DM family history hassignifi cantly lower HOMA-β levels than subject without DM family history.

scholarly journals TGF-β1 Gene Polymorphism in Codon 10 +869*C/T and Codon 25 +915*G/C Positions in Iraqi Patients with Type 2 Diabetes Mellitus

2016 ◽  
Vol 10 (2) ◽  
pp. 47-51
Author(s):  
Ihsan A. Hussein
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Protective Effect ◽  
Control Sample ◽  
Amplification Refractory Mutation System ◽  
Blood Samples ◽  
Increased Risk ◽  
Tgf Β1

This study included 50 blood samples that were collected from patients with age ranged between 35-65 years. Thirty samples were collected from patients with Type 2 Diabetes Mellitus (T2DM), while 20 blood samples were collected from healthy individuals as a control sample. The polymorphism results of TGF-β1 gene in codon 10: +869*C/T position by using amplification refractory mutation system (ARMS-PCR) showed that the T allele was suggested to have a protective effect, while C allele was associated with an increased risk of T2DM. The TT and CT were suggested to have a protective effect, while CC genotype was associated with an increased risk of T2DM. The polymorphism results of TGF-β1 gene in codon 25: +915*G/C position in samples showed that the G allele was suggested to have a protective effect, while C allele was associated with an increased risk of T2DM. The GC genotype was suggested to have a protective effect, while GG and CC genotypes were associated with an increased risk of T2DM.

Single and multi-locus association study of TCF7L2 gene variants with susceptibility to type 2 diabetes mellitus in an Iranian population

Gene ◽  
2019 ◽  
Vol 696 ◽  
pp. 88-94 ◽  
Author(s):  
Saeed Kalantari ◽  
Alireza Sharafshah ◽  
Parvaneh Keshavarz ◽  
Arash Davoudi ◽  
Razie Habibipour
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Association Study ◽  
Iranian Population ◽  
Gene Variants ◽  
Tcf7l2 Gene

Association of a common variant in TCF7L2 gene with type 2 diabetes mellitus in a Persian population

2011 ◽  
Vol 12 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Abbasali Palizban ◽  
Mahnaz Nikpour ◽  
Rasoul Salehi ◽  
Mohammad-Reza Maracy
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Common Variant ◽  
Tcf7l2 Gene
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