Serological and molecular survey of Trypanosoma evansi in Camels (Camelus dromedarius) in Maiduguri, North-east, Nigeria

2021 ◽  
Vol 42 (1) ◽  
pp. 56-62
Author(s):  
S.A. Mamman ◽  
G. Abongaby ◽  
O. Salami ◽  
J.P. Yidawi ◽  
D.A. Dakul
Keyword(s):  
Diagnostic Techniques ◽  
Trypanosoma Evansi ◽  
Surface Glycoprotein ◽  
Dromedary Camel ◽  
Serological Method ◽  
Cross Sectional ◽  
North East ◽  
Variable Surface ◽  
Pcr Targeting ◽  
Pcr Techniques

To date, camels still remain an important work animal as well as source of protein to humans in the Sudan and Sahel regions of Nigeria. Therefore, a cross-sectional study was conducted on 150 camels slaughtered in Maiduguri central abattoir to determine the prevalence of Trypanosoma evansi using Card Agglutination Test (CATT) and Polymerase Chain Reaction (PCR) techniques. Overall, 30 (20%) of the camels tested were seropositive while PCR targeting the 227 base pair of the Variable Surface Glycoprotein (VSG) gene of T. evansi detected the DNA of the parasite in 9 out of the 30seropositive camels. Higher infection was found among adult compared to the young camels using the two diagnostic techniques; 24.1% vs 19.0% and 10.3% vs 4.6%, for CATT and PCR techniques, respectively. However, the differences being not statistically significant (P > 0.05) for the two methods of diagnosis. Furthermore, significantly (P < 0.05)higher prevalence of infection was recorded among male compared to female camels using the serological method of diagnosis, while (P > 0.05) using the molecular method; 27.5% vs 13.6% for CATT and 10.1% vs 2.5% for PCR. Camels with PCV =24 %( mean: 19.8923 ± 4.0931) recorded significantly (P < 0.05) higher prevalence of 23.1% than those with PCV = 25% (mean 31.7294 ± 5.50584), where the prevalence was 17.6%.The results of this study showed that camel trypanosomosis is endemic in the study area.  Furtherstudiesto elucidate the epidemiology and socioeconomic impact of this disease in the northeast region of Nigeria are desirable. Keywords:Serology, PCR, Dromedary camel, T.evansi, Maiduguri

scholarly journals Towards a New Reference Test for Surra in Camels

2009 ◽  
Vol 16 (7) ◽  
pp. 999-1002 ◽  
Author(s):  
Thao Tran ◽  
Filip Claes ◽  
Didier Verloo ◽  
Henri De Greve ◽  
Philippe Büscher
Keyword(s):  
Antigenic Variation ◽  
Trypanosoma Evansi ◽  
Surface Glycoprotein ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Test ◽  
Perfect Agreement ◽  
Cutoff Value ◽  
Variable Surface ◽  
Variable Antigen

ABSTRACT Current serological diagnosis of Trypanosoma evansi infection in camels is based on the native variable antigen type RoTat 1.2. The goal of this study was to develop a novel serological diagnostic test based on a nonvariable protein and freed from the use of rats or mice for its production. An enzyme-linked immunosorbent assay using a recombinant extracellular domain of invariant surface glycoprotein 75 (ELISA/rISG75) was developed and tested on a collection of 184 camel sera. The results were compared to those obtained from three established antibody detection tests based on variable surface glycoprotein RoTat 1.2: an ELISA for T. evansi (ELISA/T. evansi), a card agglutination test for trypanosomiasis (CATT/T. evansi), and an immune trypanolysis (TL) assay. The ELISA/rISG75 and the ELISA/T. evansi showed a sensitivity of 94.6% (95% confidence interval [CI], 87.8 to 98.2%, at 19% positivity cutoff value) and 98.9% (95% CI, 94.1 to 99.8, at 12% positivity cutoff value), respectively. The ELISA/rISG75 had 100% specificity (CI, 95.9 to 100%), while the ELISA/T. evansi showed 98.9% specificity (CI, 95.9 to 100%). The ELISA/rISG75 demonstrated an almost perfect agreement with the TL assay, the CATT/T. evansi, and the ELISA/T. evansi, with kappa scores of at least 0.94. The ELISA/rISG75, having a performance comparable to that of the gold standard (the TL assay) and being independent of antigenic variation, may become a new reference test for surra in camels. It opens avenues for the diagnosis of T. evansi infections in other hosts as well as for the development of a pan-Trypanozoon test for detection of Trypanosoma brucei brucei, T. b. gambiense, T. b. rhodesiense, T. evansi, and T. equiperdum.

Expressed truncated N-terminal variable surface glycoprotein (VSG) of Trypanosoma evansi in E. coli exhibits immuno-reactivity

2012 ◽  
Vol 187 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
P.P. Sengupta ◽  
M. Balumahendiran ◽  
V. Balamurugan ◽  
G.R. Rudramurthy ◽  
K. Prabhudas
Keyword(s):  
Trypanosoma Evansi ◽  
Surface Glycoprotein ◽  
E Coli ◽  
Variable Surface Glycoprotein ◽  
Variable Surface

The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum

2003 ◽  
Vol 116 (3) ◽  
pp. 209-216 ◽  
Author(s):  
F. Claes ◽  
D. Verloo ◽  
D.T. De Waal ◽  
P.A.O. Majiwa ◽  
T. Baltz ◽  
...  
Keyword(s):  
Trypanosoma Evansi ◽  
Surface Glycoprotein ◽  
Variable Surface Glycoprotein ◽  
Variable Surface

scholarly journals Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1149
Author(s):  
Dina M. Metwally ◽  
Isra M. Al-Turaiki ◽  
Najwa Altwaijry ◽  
Samia Q. Alghamdi ◽  
Abdullah D. Alanazi
Keyword(s):  
Saudi Arabia ◽  
Infection Rate ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Trypanosoma Evansi ◽  
Camelus Dromedarius ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene ◽  
Polymerase Chain ◽  
Pcr Targeting

We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.

scholarly journals Malnutrition and morbidity trends in Somalia between 2007 and 2016: results from 291 cross-sectional surveys

BMJ Open ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. e033148
Author(s):  
Rocio Martin-Canavate ◽  
Estefania Custodio ◽  
Abukar Yusuf ◽  
Daniel Molla ◽  
Dominique Fasbender ◽  
...  
Keyword(s):  
Age Groups ◽  
Disease Outbreaks ◽  
Geographic Area ◽  
Complex Situation ◽  
Cross Sectional ◽  
Internally Displaced ◽  
North East ◽  
Continuous Complex ◽  
Morbidity Trends ◽  
Livelihood System

BackgroundMore than two decades of conflict and natural disasters in Somalia have resulted in one of the longest running humanitarian crises in the world. Nutrition data have been collected over the years despite challenges to inform programmatic action. This paper explores malnutrition and morbidity trends in Somalia during the last decade, disaggregated by geographical zone and livelihood system.MethodsWe used data from 291 cross-sectional surveys conducted in children aged 6–59 months between 2007 and 2016 in Somalia. Wasting, morbidity and stunting prevalences over time were analysed by geographic area, livelihood system and season. Logistic regressions were used to test trends.ResultsThe wasting trends show a striking peak in 2011, more marked in southern and central Somalia and coinciding with the famine declaration. The trend declines slightly thereafter although not consistently across all zones and livelihoods, and it raises again in 2016 especially among internally displaced persons (IDPs). Stunting declined for all groups and in all zones but with more consistent patterns in northern Somalia.Morbidity also showed a declining trend, although with multiple peaks depicting disease outbreaks.Pastoralist showed the lowest stunting estimates overall, while agrarian populations showed the lowest prevalence of wasting and morbidity. IDPs were the most affected by all outcomes. Seasonality affected the three outcomes differently by livelihood system. Stunting rates increased after the 2011 famine for all age groups within children under 5 years.ConclusionsDespite the continuous complex situation in Somalia, there has been a sustained decline in stunting and morbidity in the last decade. Wasting trends have remained at very high levels especially in north-east and the south zones of Somalia. The findings support the importance of performing trend analyses disaggregated by zone and livelihood groups within countries to better identify priorities for programme intervention.

scholarly journals Targeting the Variable Surface of African Trypanosomes with Variant Surface Glycoprotein-Specific, Serum-Stable RNA Aptamers

2003 ◽  
Vol 2 (1) ◽  
pp. 84-94 ◽  
Author(s):  
Mihaela Lorger ◽  
Markus Engstler ◽  
Matthias Homann ◽  
H. Ulrich Göringer
Keyword(s):  
Antigenic Variation ◽  
Sleeping Sickness ◽  
Rna Aptamers ◽  
Surface Glycoprotein ◽  
Variant Surface Glycoprotein ◽  
Parasite Protein ◽  
African Trypanosomes ◽  
Variable Surface ◽  
New Strategy

ABSTRACT African trypanosomes cause sleeping sickness in humans and Nagana in cattle. The parasites multiply in the blood and escape the immune response of the infected host by antigenic variation. Antigenic variation is characterized by a periodic change of the parasite protein surface, which consists of a variant glycoprotein known as variant surface glycoprotein (VSG). Using a SELEX (systematic evolution of ligands by exponential enrichment) approach, we report the selection of small, serum-stable RNAs, so-called aptamers, that bind to VSGs with subnanomolar affinity. The RNAs are able to recognize different VSG variants and bind to the surface of live trypanosomes. Aptamers tethered to an antigenic side group are capable of directing antibodies to the surface of the parasite in vitro. In this manner, the RNAs might provide a new strategy for a therapeutic intervention to fight sleeping sickness.

scholarly journals Prevalence and genetic variability of tick-borne encephalitis virus in host-seeking Ixodes ricinus in northern Italy

2009 ◽  
Vol 90 (12) ◽  
pp. 2877-2883 ◽  
Author(s):  
Giovanna Carpi ◽  
Luigi Bertolotti ◽  
Sergio Rosati ◽  
Annapaola Rizzoli
Keyword(s):  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Evolutionary Dynamics ◽  
Phylogenetic Analyses ◽  
Northern Italy ◽  
Nucleotide Sequence Analysis ◽  
Envelope Gene ◽  
North East ◽  
Tick Borne Encephalitis ◽  
Pcr Targeting

Tick-borne encephalitis (TBE) is a severe disease that has been endemic in north-east Italy since 1992. Over the past two decades, there has been an increase in the number of human cases reported in many European countries, including Italy. To assess the current TBE infection risk, questing ticks were collected from known TBE foci, as well as from a site in northern Italy where no human infections have been reported previously. A total of 1739 Ixodes ricinus (1485 nymphs and 254 adults) was collected and analysed for TBEV prevalence by a real-time RT-PCR targeting the 3′ untranslated region. Phylogenetic analyses of the partial envelope gene were conducted on two newly sequenced TBE virus (TBEV) strains and 28 previously published sequences to investigate the genealogical relationships of the circulating TBEV strains. These phylogenetic analyses confirmed a previous report that the European TBEV subtype is the only subtype circulating within the TBE foci in north-east Italy. Interestingly, nucleotide sequence analysis revealed a high degree of divergence (mean 2.54 %) between the TBEV strains recovered in the Italian province of Trento, despite the circulation of a single TBEV subtype. This elevated genetic variability within a single TBE focus may reflect local differences in the long-standing evolutionary dynamics of TBEV at this site relative to previously characterized sites, or more recent and continuous reintroduction of various TBEV strains.

SMOKELESS TOBACCO USE AND ASSOCIATED SOCIO-ECONOMIC DEMOGRAPHIC FACTORS AMONG ADULTS IN AN URBAN RESETTLEMENT COLONY OF DELHI

2021 ◽  
pp. 24-27
Author(s):  
Varun Singh ◽  
Mongjam Meghachandra Singh ◽  
Reeta Devi ◽  
Suneela Garg ◽  
Bratati Banerjee ◽  
...  
Keyword(s):  
Smokeless Tobacco ◽  
Adult Population ◽  
Cross Sectional Study ◽  
Government Employees ◽  
Cross Sectional ◽  
Change Communication ◽  
North East ◽  
Collection Data ◽  
One Year ◽  
Study Participants

Background: Smokeless tobacco (SLT) consumption poses serious health problems and is considered to be a signicant risk factor for oral, esophageal and pancreatic cancers. Estimation of the magnitude of consumption of SLT would be useful in planning for tobacco control activities in the community. Objectives: The study was conducted to estimate the prevalence of smokeless tobacco (SLT) use among adult population and to identify the socioeconomic factors associated with the SLT use among them. Methods: This was a community based cross-sectional study conducted in an urban resettlement colony of North East Delhi among adult population (>18 years age). A pre-tested interview schedule adapted from the Global Adult Tobacco Survey India (GATS India) was used for data collection. Data was analyzed using SPPS version 25. Results: A total of 440 study participants were enrolled for the study. The overall prevalence of SLT use was 24.5% (95 % CI: 20.6 - 28.8), higher in males (28.7%)(OR 1.75) as compared to females (18.7%), more in graduate or above educated (34.6%)(OR 2.02), and among government employees (48.6%)(OR 4.2)(p<0.05). The prevalence of common forms of SLT consumed were: pan masala with tobacco (15%), gutka (12.3%), zarda (10.5%), khaini (9.3%), and gul (5.2%). The most common reason for initiation of SLT use among the users was found to be seeking enjoyment 46(42.6%). Only 35 (37.6%) current SLT users planned to quit SLT in next one year of the survey. Conclusion: Nearly one fourth of the study subjects used SLT and more among males, education level graduate and above, and Government employees. This highlights the need to plan behavior change communication strategies and counsel them for reduction or cessation of SLT use

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