scholarly journals Antihyperglycemic and Antioxidant Activity of Fructus hordei Germinatus Extract on Streptozotocin-induced Diabetic Rats

2015 ◽  
Vol 14 (9) ◽  
pp. 1651-1657
Author(s):  
H Wei ◽  
X Liang ◽  
B Wu ◽  
J Zhang ◽  
Y Qin ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Dependent Manner ◽  
Liver And Kidney ◽  
Plasma Insulin Levels ◽  
Antioxidant Markers

Purpose: To explore the antihyperglycemic and antioxidant effects of Fructus Hordei Germinatus extract (FHGE) in diabetic rats.Methods: The aqueous extract of FHG was obtained by steeping the dried Fructus Hordei Germinatus in water at 60oC three times, each for 1 h before first drying in an oven and then freeze-drying the last extract thus obtained. Antihyperglycemic effect was measured by blood glucose and plasma insulin levels. Oxidative stress was evaluated in liver and kidney by antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT), while blood serum levels of creatinine and urea were also determined in both diabetic control and treated rats.Results: Compared with diabetic rats, oral administration of FHGE at a concentration of 20, 40 and 80 mg/kg daily for 45 days showed a significant decrease in fasting blood glucose (p < 0.05) and increased insulin (p < 0.05) level,. Furthermore, it significantly reduced biochemical parameters (serum creatinine and serum urea, p < 0.05)). The treatment also resulted in significant increase in GSH, GPx (p < 0.05), SOD and CAT (p < 0.05), as well as decreased LPO (p < 0.05) level in the liver and kidney of diabetic rats.Conclusion: The results suggest that FHGE may effectively normalize the impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. FHGE has a protective effect against lipid peroxidation by scavenging free radicals and is thus capable of reducing the risk of diabetic complications.Keywords: Fructus hordei Germinatus, Diabetic, Antihyperglycemic, Lipid peroxidation, Antioxidant, Fasting blood glucose, Creatinine, Urea

scholarly journals Polygonatum sibiricum extract exerts inhibitory effect on diabetes in a rat model

2021 ◽  
Vol 18 (7) ◽  
pp. 1493-1497
Author(s):  
Lai-zeng Yu ◽  
Xue-peng Zhang ◽  
Ying-xin Wang
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Dependent Manner

Purpose: To investigate the effect of Polygonatum sibiricum extract (PSE) on streptozotocin-induced diabetic rats. Methods: PSE was obtained by steeping the dried Polygonatum sibiricum in water at 60 oC three times, each for 1 h, before first drying in an oven at 100C and then freeze-drying the final extract, thus obtained. Diabetic model rats were prepared by a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control, normal control, reference (glibenclamide1 mg/kg) as well as PSE groups, (35, 70 and 140 mg/kg). Blood glucose and plasma insulin levels were measured to determine antihyperglycemic effect. Oxidative stress was evaluated in liver and kidney by their antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats. Results: Compared with diabetic rats, oral administration of PSE at a concentration of 120 mg/kg daily for 30 days showed a significant decrease in fasting blood glucose (118.34 ± 3.29 mg/dL) (p < 0.05) and increased insulin level (12.86± 0.62 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.83 ±0.21 mg/dL, p < 0.05) and serum urea (43.26±1.42 mg/dL, p < 0.05). Conclusion: The results suggest that PSE may effectively normalize impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. Thus, PSE has a protective effect against lipid peroxidation by scavenging free radicals, restoration of insulin function, and reduction of the incidence of complications.

scholarly journals Antidiabetic and Antinephropathic Potential of Ajwa Pit & Pulp (Phoenix dactylifera) in Alloxanized Diabetic Rats

2020 ◽  
Vol 34 (1) ◽  
pp. 39-43
Author(s):  
Imran Maqsood Butt
Keyword(s):  
Blood Glucose ◽  
Creatinine Clearance ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Phoenix Dactylifera ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Medical Institute ◽  
Urine Creatinine

Introduction: Generally in the world, diabetes is the most significant cause of deaths and morbidity. Blood glucose lowering agents possess remarkable adverse effects like weight gain and hypoglycemia and thus hindering the achievement of glycemic control. Since earliest times till today, herbal preparations have been utilized for management of diabetes and its complications. Aims & Objectives: To investigate antihyperglycemic and nephroprotective effects of Ajwa pit and pulp in alloxanized - diabetic and nephropathic rats. Place and duration of study: This study was conducted at Postgraduate Medical Institute, Lahore for the duration of four weeks. Material & Methods: Adult male rats (weighing 120-150 g), total of 32 were purchased and divided into 4 groups. The group1 was normal control whereas group2 was diabetic control. Diabetes was induced in groups 2, 3 and 4 with 150mg/kg single alloxan monohydrate1.P Injection. Groups 3 and 4 were given Ajwa pit and pulp rations for 4 weeks. Blood and urine samples were taken on zero and 4 weeks for fasting blood glucose, serum and urine biochemical variables for renal functions. Results: The results revealed that Ajwa pits significantly decreased serum levels of glucose (252±60 vs. 348±67 mg/dl), serum urea (38±7.0 vs. 63±9.6 mg/dl), serum creatinine (0.39±0.1 vs. 0.58±0.1 mg/dl) and microalbuminuria marker (7.8±1.7 vs. 9.0±2.2mg/l). Moreover it improved urine creatinine (14.0±2.9 vs.10.0±2.7 mg/dl) and creatinine clearance (0.226±0.08 vs 0.117±0.04 ml/min) when compared to diabetic control rats. Effect of Ajwa pulp was far less than Ajwa pit. Ajwa pulp did not significantly decrease serum levels of glucose (290±60 vs. 348±67 mg/dl), urea (51±9.5 vs. 63±9.6 mg/dl), creatinine (0.66±0.2 vs. 0.58±0.1 mg/dl) and microalbuminuria marker (8.3±2.0 vs. 9.0±2.2mg/l). Also there was no improvement in urine creatinine (11.1±1.6 vs. 10.0±2.7 mg/dl) and creatinine clearance (0.130±0.05 vs 0.117±0.04 ml/min) levels when compared to diabetic control rats. Conclusion: Ajwa pit possesses strong antihyperglycemic and protective effect in renal damage by prolonged diabetes mellitus. Highest polyphenols, bioflavonoids and antioxidants are presumed to be responsible for this effect.

Effect of Resistance and Endurance Training on Gene Expression of Adiponectin and its Receptors (AdipoR1 and AdipoR2) in Testicles and Serum Levels of Sex Hormones in Diabetic Rats

2020 ◽  
Author(s):  
Zahra Nadi ◽  
Mohammad Bayat ◽  
hadi karami ◽  
Mohammad Parastesh ◽  
parvindokht bayat
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Endurance Training ◽  
Sex Hormones ◽  
Fasting Blood Glucose ◽  
Serum Testosterone ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Adiponectin Gene ◽  
Testosterone Levels

Abstract Background Sex hormone, adiponectin and its receptors interacted in the testes. Diabetes can also interfere with this interaction. Regular exercise has some effects on the diabetes side effects. This study purpose was to investigate the effects resistance and endurance training on adiponectin gene expression and its receptors and sex hormones in those rats with diabetes.MethodsIn this experimental study, 48 male Wistar rats were divided into 6 groups by random. After performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone.Results The induction of diabetes mellitus STZ-NA significantly increased the serum level of fasting blood glucose, the gene expression of adiponectin and also the AdipoR 1 in the testicles of diabetic rats in comparison with healthy controls. In addition, diabetes resulted in a significant reduction in serum testosterone levels and LH in diabetic group, but it has no effect on FSH.Conclusions Resistance and endurance training decreased the blood glucose with a significant amount reduction in adiponectin levels and AdipoR 1 gene rats, and also increased the serum testosterone levels and LH in diabetic rats. Resistance and endurance training can improve expression of adiponectin and AdipoR 1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.

scholarly journals BANANA PEEL FLAKES ALLEVIATE BLOOD GLUCOSE AND STRESS IN A DOSE-DEPENDENT MANNER

2020 ◽  
pp. 75-81
Author(s):  
ANDREANYTA MELIALA ◽  
YUSTINA ANDWI ARI SUMIWI ◽  
PARAMITA NARWIDINA ◽  
SRI LESTARI SULISTYO RINI ◽  
WIDIASTUTI SETYANINGSIH
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Banana Peel ◽  
Standard Diet ◽  
Dependent Manner ◽  
Immobility Time ◽  
Healthy Control

Objective: This study aimed to evaluate the antidiabetic and antidepressant effects of banana peel flakes in streptozotocin-induced diabetic rats. Methods: Twenty-five male Wistar rats were classified into five groups with different treatments. Groups I to IV were diabetic rats model groups that consumed only standard diet, standard diet containing 5%, 10%, and 20% of banana peel flakes, respectively. While group V was a healthy control group fed a standard diet. Immunohistochemistry staining was measured to examine serotonin expression in the colon and pancreas. Results: The diabetic rats treated with 20% banana peel flakes had a lower blood glucose concentration (p<0.05) compared with diabetic control and showed a shorter duration of immobility time (p<0.05) than the healthy control. Additionally, compared with diabetic control, the diabetic rats treated with 5% banana peel flakes showed higher serotonin expression (p<0.05) in the colon. In contrast, serotonin expression in the pancreas did not show any significant difference (p>0.05). Conclusion: The present study disclosed that the banana peel flakes provided an antidepressant effect in the diabetic rats model, which might occur through the mechanism of controlling blood glucose concentration.

scholarly journals Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

2015 ◽  
Vol 35 (4) ◽  
pp. 1326-1334 ◽  
Author(s):  
Saritha Marella ◽  
Dilip Rajasekhar Maddirela ◽  
Kameswara Rao Badri ◽  
Malaka Venkateshwarulu Jyothi Kumar ◽  
Apparao Chippada
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Protective Effects ◽  
Tissue Lipid ◽  
Lipid Levels ◽  
Liver And Kidney ◽  
Streptozotocin Induced Diabetes ◽  
Wistar Albino Rats

Background: This study was aimed to evaluate the protective effects of a novel anti-hyperglycemic “Mcy protein” isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model. Materials and Methods: Wild type and Streptozotocin induced diabetic male wistar albino rats were either treated with single intraperitoneal injection of 2.5 mg Mcy protein/kg body weight or acetate buffer daily for 30 days. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests. Results: Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. Our studies also showed the pancreatic islet regeneration in Mcy treated rats. Conclusion: Mcy protein can alleviate hyperlipidemia and help manage diabetes by stimulating insulin secretion without evident toxic effects on liver and kidney.

scholarly journals Hypoglycaemic and Hypolipidemic Effects of Treculia africana Aqueous Leaves Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
Idris A. Kankara ◽  
Gayus A. Paulina ◽  
M. Aliyu
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Treculia Africana

This study investigated the hypoglycaemic and hypolipidaemic effects of Treculia africana plant used in Nigeria as medicinal plant. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Twenty five (25) male albino rats were divided into five groups, five (5) rats per group; normal control, diabetic control and diabetic groups treated with aqueous leaves extract of 200,400 and 800 mg/Kg body weight respectively for 21 days orally. The effects of the extract on some biochemical parameters were evaluated; fasting blood glucose level was assayed using glucose oxidase method, total cholesterol and HDL –cholesterol were assayed using enzymatic method while LDL- cholesterol was determined by Friedewald equation. The results showed that, extract significantly (p<0.05) decrease the elevated fasting blood glucose levels, total cholesterol, triglyceride and LDL- cholesterol when compared with the diabetic control rats. The extract also caused significant (p<0.05) increased in HDL –cholesterol and body weight when compared with diabetic control rats. Aqueous leave extract of Treculia africana possess hypoglycemic effect and the most effective dose was 800 mg/Kg body weight in amelioration of hyperglycaemia and most all toxicity effects of alloxan on lipid profile.

scholarly journals Modulations of Some Carbohydrate Metabolic Enzymes by Aqueous and Ethanol Buchholzia coriacea Seed Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
K. O. Gbagbeke ◽  
A. O. Naiho ◽  
P. R. C. Esegbue ◽  
M. O. Odigie ◽  
M. A. Omoirri
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Metabolic Enzymes ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Total Carbohydrate ◽  
Adult Rats ◽  
Ethanol Extracts

Characterised by abnormal increase in blood glucose level, Diabetes is a metabolic disorder that is associated with complications in carbohydrate, protein and fat metabolism. In recent times, medicinal herbs have been implicated in traditional medical practice for the treatment of this ailment. Studies have shown that Buchholzia coriacea seed possesses some anti-hyperglycemic properties that may be useful in the management of diabetes. To this point, present study investigated the effect(s) of oral administration of aqueous and ethanol extracts of Buchholzia coriacea on some carbohydrate metabolism parameters in normal and alloxan-induced diabetic rats. Forty (40) adult rats of both sexes were randomly assigned into two groups (normoglycemic and hyperglycemic). While group 1 (normoglycemic) had normal control, metformin, aqueous extract (250mg/kg) and ethanol extract (250 mg/kg) treated sub-groups respectively, Group 2 (hyperglycemic) contained the diabetic control, metformin, aqueous extract (250 mg/kg), and ethanol extracted (250mg/kg) treated sub-groups dosed daily by oral gavage for 14 days. At the end of the treatment, rats were euthanized via cervical dislocation; blood samples were collected by cardiac puncture for statistical analysis. One-way analysis of variance (ANOVA) revealed that dosing with extracts had insignificant effect(s) on body weight of rats. Fasting Blood Glucose (FBG) levels were elevated before and after extracts administration. Metformin, aqueous and ethanol extracts significantly reduced (p<0.05) FBG levels. Also, compared with control, total carbohydrate, liver glucose, glycosylated haemoglobin, Lactate Dehydrogenase, Isocitrate dehydrogenase, MDH, SDH, 6-phosphogluconate dehydrogenase, G6PD and CcO activities were significantly reduced (p<0.05) in diabetic treated rats. Buccholzia Coriacea was therefore seen to pose hypoglycemic and glycolytic effects, regulating activities of carbohydrate metabolic enzymes. Apparently, there is a scientific merit in the use of the extract in the management of diabetes.

scholarly journals Evaluation ofMallotus oppositifoliusMethanol Leaf Extract on the Glycaemia and Lipid Peroxidation in Alloxan-Induced Diabetic Rats: A Preliminary Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
C. O. Nwaehujor ◽  
I. I. Ezeigbo ◽  
F. C. Nwinyi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Treatment ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective. Mallotus oppositifolius(Geiseler) Müll. Arg. (Euphorbiaceae) is folklorically used to “treat” diabetic conditions in some parts of Nigeria therefore the study, to investigate the extract of the leaves for activities on hyperglycaemia, lipid peroxidation, and increased cholesterol levelsin vivoin alloxan diabetic rats as well as its potential antioxidant activityin vitro.Methods. Albino rats (240–280 g) were given an injection of 120 mg/kg body weight, i.p. of alloxan monohydrate. After 8 days, diabetic animals with elevated fasting blood glucose levels (>9 mmol/L) were considered and selected for the study.Results. Oral treatment with the extract administered every 12 h by gavage at doses of 100, 200, and 400 mg/kg of the extract to the test rats, for 14 days, resulted in a significant dose-dependent decrease in blood glucose levels from 12.82 ± 1.02 mmol/dL to 4.92 ± 2.01 mmol/dL at the highest dose of 400 mg/kg compared to the control drug and glibenclamide as well as attendant significant decline in diabetic rats employed in the study.Conclusion. The extract also showedin vitroconcentration-dependent antioxidant activity following the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing assays. Findings further suggest the presence of active antidiabetic and antioxidant principles inM. oppositifoliusleaves.

scholarly journals ANTI-HYPERGLYCEMIC EFFECT OF TERMINALIA CATAPPA FRUIT EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 9 (4) ◽  
pp. 212 ◽  
Author(s):  
Tapan Behl ◽  
Anita Kotwani
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Urine Volume ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Dependent Manner ◽  
Fruit Extract ◽  
Terminalia Catappa ◽  
Hyperglycemic Effect

Objective: To explore the anti-hyperglycemic effect of fruit extract of Terminalia catappa (Indian almond), a potential medicine from plant origin in a diabetic rat model.Methods: Streptozotocin-induced chronic diabetic rat model was utilized in the study. Three doses of test drug, hydro-alcoholic fruit extract of Terminalia catappa in 20 mg/kg, 30 mg/kg and 40 mg/kg and a standard anti-diabetic drug, glibenclamide (10 mg/kg) was used. The study had a total of nine groups with eight animals in each group. Drugs were given orally every day for 12 w. Blood glucose, body weight and urine volume were measured weekly, glycosylated hemoglobin (HbA1c) was estimated at 12th week in all groups. Data for all parameters were analyzed using one-way ANOVA repeated measures followed by Mann-Whitney test.Results: Hydro-alcoholic fruit extract of T. catappa significantly decreased blood glucose, urine volume and increased body weight in a dose-dependent manner in diabetic rats. At 12th week, blood glucose level in control, diabetic control, glibenclamide, T. catappa (40 mg/kg) group was 96.25±2.05 mg/dl, 599.75±0.25 mg/dl, 248.25±11.45 mg/dl, 115.00±3.78 mg/dl respectively. Effect of T. catappa in 30 mg/kg and 40 mg/kg dose was significantly more than glibenclamide. At 12th week, HbA1c level in control, diabetic control, glibenclamide, T. catappa (40 mg/kg) was 2.94±0.33 mmol/l, 4.94±0.49 mmol/l, 3.61±0.28 mmol/l, 3.21±0.27 mmol/l. Treatment with T. catappa 30 mg/kg, 40 mg/kg and glibenclamide brought back the level of HbA1c to normal levels. The addition of glibenclamide to T. catappa (40 mg/kg) did not produce any additional effect on blood glucose and HbA1c levels compared to the effect of T. catappa (40 mg/kg) in diabetic rats.Conclusion: Terminalia catappa fruit extract exhibited a significant anti-hyperglycemic effect in diabetic rats and has a great potential to be used in diabetes.

scholarly journals Antihyperlipidemic Effect of a Polyherbal Mixture in Streptozotocin-Induced Diabetic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ahmad Ghorbani ◽  
Reza Shafiee-Nick ◽  
Hassan Rakhshandeh ◽  
Abasalt Borji
Keyword(s):  
Blood Glucose ◽  
Nigella Sativa ◽  
Juglans Regia ◽  
Fasting Blood Glucose ◽  
Punica Granatum ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Beneficial Effects

The effects of a polyherbal mixture containingAllium sativum,Cinnamomum zeylanicum,Citrullus colocynthis,Juglans regia,Nigella sativa,Olea europaea,Punica granatum,Salvia officinalis,Teucrium polium,Trigonella foenum,Urtica dioica, andVaccinium arctostaphyloswere tested on biochemical parameters in diabetic rats. The animals were randomized into three groups: (1) normal control, (2) diabetic control, and (3) diabetic rats which received diet containing 15% (w/w) of this mixture for 4 weeks. Diabetes was induced by intraperitoneal injection of streptozotocin (55 mg/kg). At the end of experiment, the mixture had no significant effect on serum hepatic enzymes, aspartate aminotransferase, and alanine aminotransferase activities. However, the level of fasting blood glucose, water intake, and urine output in treated group was lower than that in diabetic control rats (P<0.01). Also, the levels of triglyceride and total cholesterol in polyherbal mixture treated rats were significantly lower than those in diabetic control group (P<0.05). Our results demonstrated that this polyherbal mixture has beneficial effects on blood glucose and lipid profile and it has the potential to be used as a dietary supplement for the management of diabetes.

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