scholarly journals Callus Induction and Plantlet Regeneration Systems in Indica Rice (Oryza sativa L.) Cultivar Sangyod

2017 ◽  
Vol 15 (10) ◽  
pp. 753-763
Author(s):  
Thi Linh HO ◽  
Sompong TE-CHATO ◽  
Sureerat YENCHON
Keyword(s):  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Casein Hydrolysate ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Mature Embryos ◽  
Spot Formation ◽  
Number Of Shoots

This study was conducted to determine the optimum concentrations of 2, 4-D, L-proline and casein hydrolysate (CH) for efficient callus induction and plantlet regeneration from culturing mature embryos of Sangyod, an economically important indica rice variety in Thailand. The highest frequency of callus induction (73.08 ± 2.65 %) was obtained from MS medium supplemented with 2 mg/L 2,4-D, 750 mg/L CH and 200 mg/L L-proline. The combination of 1 mg/L BA, 0.5 mg/L Kn and 0.5 mg/L NAA containing solidified MS medium gave the maximum mean fresh weight of callus (938.9 ± 44 mg), green spot formation (64.17 ± 7.08 %), shoot induction frequency (66.25 ± 6.80 %) and mean number of shoots/explant (6.12 ± 0.36 shoots). The greatest mean number of shoots/explant (14.93 ± 0.97 shoots) and root formation percentage (82.71 ± 3.03 %) was observed in liquified MS medium supplemented with 0.5 mg/L NAA and 1 mg/L BA.

scholarly journals Effect of N-methyl-N-nitro-N-nitrosoguanidine (NTG) on callus induction and survival rate on mature embryos of beans (Phaseolus vulgaris)

2009 ◽  
Vol 3 (2) ◽  
pp. 91-98
Author(s):  
Sattar A. Shlahi ◽  
Zahra N. Hashim Al- Hattab
Keyword(s):  
Survival Rate ◽  
Callus Induction ◽  
Indole Acetic Acid ◽  
Casein Hydrolysate ◽  
Dry Weight ◽  
Ms Medium ◽  
Fresh Weight ◽  
Benzyl Adenine ◽  
Mature Embryos ◽  
Survival Percentage

This research was conducted to study the effect of the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine on the percentage of callus induction and survival from mature beans embryos harvester cultivar. Seeds were treated with (0.2 or 0.4) millimolar of the mutagen NTG in combination with 0.0, 4 or 8% of ethanol, pH 5 ±2 0. for 24 h. Calli were induced on mature embryos by using MS medium with 0.5 mg/l of Benzyl adenine (BA), 1 mg/l Indole acetic acid (IAA) and 100 mg/l from each of Casein hydrolysate, Glycine, Asparagine, Tyrosine, and Myo-Inositol. Results showed that the hypocotyl surpassed the radical and the plume significantly in terms of survival reached 56.3%. Mutagen treatments showed asignificant effect on calli survival. Treatment with 8% Ethanol was lethal for all explants. While treatment with 0.4 mM NTG without Ethanol gaved the highest survival rate. The interaction between the treatments and the explants showed that the lowest survival percentage was which 8.8% that was for shoots treated with 0.2 mM of 4% Ethanol. Calli induced on hypocotyls treated with 0.4 mM NTG without Ethanol gave the highest fresh weight (347.2) mg while the lowest was (60) mg for calli induced on the radical treated with 0.4 mM NTG with 4% Ethanol. Moreover the highest dry weight was 22.5 mg for calli induced from hypocotyls treated with 0.4 millimolar NTG without Ethanol that was higher than the control 17.2 mg.The lowest dry weight obtained from calli induced on the radical treated with 0.4 mM NTG with 4% Ethanol was 3 mg. In conclusion the results showed that 0.4 mM NTG without Ethanol gave the highest survival rate and the highest fresh and dry weight for calli induced on the hypocotyl.

scholarly journals Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

2017 ◽  
Vol 18 (2) ◽  
pp. 91
Author(s):  
Kunti Anis Azizah ◽  
Didik Pudji Restanto ◽  
Bambang Sugiharto
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Medium Composition ◽  
Regeneration Frequency ◽  
Optimum Result

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

scholarly journals In Vitro Regeneration of High Yielding Indica Rice (Oryza sativa L.) Varieties

2012 ◽  
Vol 5 (1) ◽  
pp. 173-177 ◽  
Author(s):  
MJ Alam ◽  
M Imran ◽  
L Hassan ◽  
MH Rubel ◽  
M Shamsuddoha
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Culture Media ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Induction Frequency ◽  
Establishment Rate ◽  
Number Of Shoots

The experiment was conducted to observe the regeneration potential and also to establish a suitable in vitro plantlet regeneration protocol from mature seed derived embryogenic calli of four indica rice varieties viz BRRI dhan28, BRRI dhan29, BRRI dhan47 and Binadhan-7 after partial desiccation treatment. Different concentrations and combinations of growth regulators were used in MS medium to observe the callus induction ability using mature embryo as explants. The percentage of callus induction frequency  was highest (86.00) in BRRI dhan47 and the lowest (56.50) in Binadhan-7 Among the culture media the performance of MS +500 mgl-1 L- Proline + 2.0 mgl-12, 4-D + 0.8 mgl-1BAP was better than any other media for callus induction frequency (%), rapid  callusing, size of the callus (mm), texture of callus and color of callus. Among the four varieties, shoot regeneration was highest in BRRI dhan29 (84.33%) which required minimum (14.80) days to and the lowest was in Binadhan-7 (39.67%) which required maximum (15.47) days. Among the treatments, the highest (65.75%) shoot regeneration was observed with MS + 6.0 mgl-1Kn +0.5 mgl-1 NAA which required minimum days (13.75) to develop green bud formation and the lowest shoot regeneration (56.50) was observed with MS + 2.0 mgl-1Kn + .05 mgl-1 NAA. BRRI dhan29 produced more number of shoots (4.67) per callus while Binadhan-7 showed minimum number of shoots (2.87) per callus. The highest number of shoot producing roots (3.66) was observed in BRRI dhan29 which showed maximum number of root per plant and the lowest (3.11) in Binadhan-7. Among the three treatments MS + 0.6 mgl-1IBA showed highest percentage 86.67 of root followed by MS+ 0.6 mgl-1IBA 70% and the lowest (66.7%) was in MS+ 0.4 mgl-1IBA. The establishment rate of the plantlet in the pot was the highest (67.67%) in BRRI dhan29 and Binadhan-7 showed lowest establishment rate (51.22%) in pot. DOI: http://dx.doi.org/10.3329/jesnr.v5i1.11573J. Environ. Sci. & Natural Resources, 5(1): 173 - 177, 2012

scholarly journals Optimization of selected plant growth regulators on callus induction of Oryza sativa L. var MR 219

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 38-45
Author(s):  
Siti Nur Adila H. ◽  
Nor Azma Y.
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Crop Improvement ◽  
Naphthalene Acetic Acid

MR 219, an indica rice from a cross between MR 137 and MR 151 is a long-grain rice variety of high-yielding, good quality of shape and taste, short maturation as well as resistance to blast and bacterial leaf blight. However, it is regarded as a recalcitrant variety due to its low ratio of callogenesis and regeneration in in vitro culturing. This would be a hindrance to crop improvements such as genetic transformation and other crop improvement methods. The objective of this study was to optimize the concentrations and combinations of plant growth regulators (PGRs) which were 2,4-Dichlorophenoxyacetic acid (2,4-D), Kinetin (KIN) and Naphthalene acetic acid (NAA) on its callus induction that ranging from 0.5 to 15.0 mg/L. The callus was induced from the mature seeds of MR 219 on the Murashige and Skoog (MS) media supplemented with 4.4 g/L of MS powder with vitamins, 30.0 g/L of sucrose and 3.5 g/L of gelrite with the addition of 2,4-D, KIN and NAA in different concentrations and combinations. All media supplemented with 2,4- D had successfully induced the callus and 2.0 mg/L of 2,4-D was the best concentration for the callus induction with a 100% success rate. The addition of 15.0 mg/L of NAA, provided less time taken for callus induction with better callus morphology which resulted in fewer browning problems.

High-Efficiency Plant Regeneration From Callus Induced on Mature Indica Rice Caryopses

1995 ◽  
Vol 43 (3) ◽  
pp. 337 ◽  
Author(s):  
R Kunanuvatchaidach ◽  
ID Godwin ◽  
SW Adkins
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
High Efficiency ◽  
Oryza Sativa L ◽  
Callus Formation ◽  
Indica Rice ◽  
Casein Hydrolysate ◽  
Plantlet Regeneration ◽  
Naphthaleneacetic Acid ◽  
Plantlet Formation

High frequency callus production was achieved following the culturing of mature caryopses on Murashige and Skoog (MS) medium containing MS vitamins, 10 then 5 μM 2,4-dichlorophenoxyacetic acid, 59 mM sucrose, 3 g L-1 casein hydrolysate and 8 g L-1 agar. Six south-east Asian Indica rice (Oryza sativa L.) cultivars produced embryogenic structures after 6 weeks of culture at 26 ± 1°C. Efficiency in embryogenic response of genotypes differed, Kurkaruppan = FR13A > Khao Dawk Mali 105 = Nam Sagui 19 = RD7 > Pin Gaew 56. Additives such as mannitol, tryptophan and ABA nor manipulation of light environment improved callus initiation or embryogenic callus formation. The use of a number of different plantlet regeneration media either free of plant growth regulators or containing auxins (indole-3-acetic acid and α-naphthaleneacetic acid), cytokinins (kinetin and 6-benzylaminopurine) and an ethylene producing chemical (1 -aminocyclopropane carboxylic acid) showed that the plant: growth regulator-free medium or the one supplemented with 1-aminocyclopropane carboxylic acid (5 μM) and kinetin mu 5 μM) were best for plantlet formation. However, a single recommendation could not be made for all cultivars. One further culture modification involving a mild osmotic stress (polyethylene glycol 100 g L-1) in R-2 liquid medium enhanced plantlet regeneration in Nam Sagui 19 two-fold but Khao Dawk Mali 105 was recalcitrant to this treatment. Plantlets (700) produced from all treatments subsequently developed into plants, most of which were morphologically normal, in the greenhouse.

scholarly journals Plant Regeneration from Mature Embryo Derived Callus of Basmati Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 45-50
Author(s):  
MS Ferdous ◽  
MA Samad ◽  
MS Haque ◽  
SA Mony
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Root Elongation ◽  
Rice Variety ◽  
Mature Embryo ◽  
Basmati Rice ◽  
Root Induction ◽  
Ms Medium ◽  
Constant Concentration ◽  
Mature Embryos

The experiment was conducted with mature embryos of an aromatic rice variety (Basmati 370) to investigate the appropriate growth condition for callus induction as well as plant regeneration. Different concentrations of 2,4-D (0.0, 1.5, 2.5, 3.5, 4.5 mgL-1) in MS medium were used for callus induction. Among the concentrations of 2,4-D, 2.5 mg-1 was the best concentration for callus induction (95.83%). Different concentrations of Kinetin (0.0, 8.0, 10.0, 12.0, 14.0 mgL-1) with a constant concentration of NAA (0.5 mg-1) were used for plant regeneration from callus. The combination of 10.0 mg-1 Kinetin and 0.5 mg-1 NAA was found to be the best for shoot regeneration (77.50%). Different concentrations of MET (1.5, 2.5, 3.5 mg-1) in MS medium with a constant concentration of NAA (0.5 mg-1) were used for root induction. A combination of 2.5 mg-1 MET and 0.5 mg-1 NAA was found most efficient for root induction (85.00%) with higher number of roots per plant (19.50) and better root elongation (1.88 cm). The plantlet was survived well in the plastic pot as well in the earthen pot or in the field.DOI: http://dx.doi.org/10.3329/pa.v19i2.16921 Progress. Agric. 19(2): 45 - 50, 2008

scholarly journals In vitro propagation of six selected sugarcane mutant clones through leaf explants

2021 ◽  
Vol 883 (1) ◽  
pp. 012075
Author(s):  
R Purnamaningsih ◽  
D Sukmadjaja ◽  
S Suhesti ◽  
S Rahayu
Keyword(s):  
Callus Induction ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
Culture Techniques ◽  
High Productivity ◽  
Media Formulation ◽  
Number Of Shoots

Abstract Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.

scholarly journals In vitro regeneration performance of Corchorus olitorius

1970 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
M Hoque ◽  
KM Nasiruddin ◽  
GKMN Haque ◽  
GC Biswas
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Root Formation ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Corchorus Olitorius ◽  
The Media ◽  
Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

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