scholarly journals In Vitro Antifungal Drug Susceptibility Testing of Aspergillus Isolates from Clinical and Environmental Samples

2018 ◽  
Vol 5 (4) ◽  
pp. 154-158
Author(s):  
Raksha LNU ◽  
Gurjeet Singh ◽  
Anant D Urhekar
Keyword(s):  
Environmental Samples ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug

scholarly journals Etiological prevalence and antifungal sensitivity patterns of dermatophytosis in India – A multicentric study

2021 ◽  
Vol 0 ◽  
pp. 1-7
Author(s):  
Sushil Tahiliani ◽  
Abir Saraswat ◽  
A. K. Lahiri ◽  
Avani Shah ◽  
Dinesh Hawelia ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Geographical Area ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug ◽  
Tinea Corporis ◽  
Fungal Culture ◽  
Multicentric Study

Background: The prevalence of dermatophytes varies with season, geographical area, socio-economic factors and effective management strategies. Aims: The aim of the study was to assess the prevalence of pathogenic dermatophytes, clinical types of dermatophyte fungal infection, and in vitro antifungal drug susceptibility testing against dermatophytes. Methods: Three hundred and ninety five patients with dermatophytosis were enrolled from five cities (Mumbai, Delhi, Lucknow, Kolkata and Hyderabad) across India. All patients were subjected to clinical examination and investigations, including potassium hydroxide microscopy, fungal culture and antifungal drug susceptibility testing. Results: Trichophyton rubrum was the most common species identified (68.4%), followed by T. mentagrophytes (29.3%). Within species, T. mentagrophytes was prevalent in humid environmental conditions (Mumbai and Kolkata), whereas T. rubrum was prevalent in noncoastal areas (Delhi, Lucknow and Hyderabad). Tinea corporis (71.4%) and tinea cruris (62.0%) were the common clinical types observed. antifungal drug susceptibility testing data indicated that minimum inhibitory concentration required to inhibit the growth of 90% of organisms (MIC-90) was lowest for griseofulvin (0.25–3.0 μg/mL). Among oral antifungals, the mean MIC of itraconazole was within the range (0.84 [0.252] μg/ mL), whereas high mean MIC values were reported for terbinafine (0.05 [0.043] μg/mL). Among topical agents, lowest mean MIC values were reported for luliconazole (0.29 [0.286] μg/mL), eberconazole (0.32 [0.251]) μg/mL and amorolfine (0.60 [0.306]) μg/mL. Limitations: Lack of correlation between in vitro antifungal susceptibility and clinical outcome and absence of defined MIC breakpoints. Conclusion: T. rubrum was the most common, followed by T. mentagrophytes as an emerging/codominant fungal isolate in India. Tinea corporis was the most common clinical type of dermatophytosis. Mean MIC of terbinafine was above the reference range, while it was within the range for itraconazole; griseofulvin had the lowest mean MIC. Luliconazole presented the lowest mean MIC values across cities.

scholarly journals Pyrazinamide Is Active against Mycobacterium tuberculosis Cultures at Neutral pH and Low Temperature

2016 ◽  
Vol 60 (8) ◽  
pp. 4956-4960 ◽  
Author(s):  
Alice L. den Hertog ◽  
Sandra Menting ◽  
Richard Pfeltz ◽  
Matthew Warns ◽  
Salman H. Siddiqi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Low Temperature ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Acidic Ph ◽  
Content Type ◽  
The Past ◽  
Neutral Ph

ABSTRACTFor the past decades, an acidic pH has been used to renderMycobacterium tuberculosissusceptible to pyrazinamide forin vitrotesting. Here, we show that at the standard breakpoint concentration and reduced culture temperatures, pyrazinamide (PZA) is active against tuberculosis (TB) at neutral pH. This finding should help unravel the mechanism of action of PZA and allow drug susceptibility testing (DST) methods to be optimized.

Antifungal drug susceptibility testing and resistance in Aspergillus

1999 ◽  
Vol 2 (5) ◽  
pp. 326-334 ◽  
Author(s):  
David W. Warnock ◽  
Beth A. Arthington-Skaggs ◽  
Ren-Kai Li
Keyword(s):  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug

scholarly journals Clinical implications of antifungal drug susceptibility testing of dermatophytes

2019 ◽  
Vol 10 (6) ◽  
pp. 737
Author(s):  
Ananta Khurana ◽  
Kabir Sardana ◽  
Anuradha Chowdhary ◽  
Khushboo Sethia
Keyword(s):  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antifungal Drug ◽  
Clinical Implications

scholarly journals New Approach for Drug Susceptibility Testing: Monitoring the Stress Response of Mycobacteria

2009 ◽  
Vol 53 (11) ◽  
pp. 4598-4603 ◽  
Author(s):  
Ronald J. Rieder ◽  
Zhihui Zhao ◽  
Boris Zavizion
Keyword(s):  
Susceptibility Testing ◽  
Physiological Stress ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Bacterial Suspension ◽  
Prolonged Incubation ◽  
New Approach ◽  
Induced Stress ◽  
In Vitro Drug Susceptibility

ABSTRACT Methods currently used for in vitro drug susceptibility testing are based on the assessment of bacterial growth-related processes. This reliance on cellular reproduction leads to prolonged incubation times, particularly for slowly growing organisms such as mycobacteria. A new rapid phenotypic method for the drug susceptibility testing of mycobacteria is described. The method is based on the detection of the physiological stress developed by susceptible mycobacterial cells in the presence of an antimicrobial compound. The induced stress was quantified by differential monitoring of the dielectric properties of the bacterial suspension, an easily measurable electronic property. The data presented here characterize the stress developed by Mycobacterium tuberculosis cells treated with rifampin (rifampicin), isoniazid, ethambutol, and pyrazinamide. Changes in the dielectric-based profiles of the drug-treated bacteria revealed the respective susceptibilities in near real time, and the susceptibilities were well correlated with conventional susceptibility test data.

Sign in / Sign up

Export Citation Format

Share Document