scholarly journals ASSESSMENT OF STROMAL AND INTRA-EPITHELIAL TUMOR INFILTRATING LYMPHOCYTES IN COLORECTAL CARCINOMA AT ARMED FORCES INSTITUTE OF PATHOLOGY

2021 ◽  
Vol 71 (2) ◽  
pp. 392-95
Author(s):  
Amna Ameer ◽  
Farhan Akhtar ◽  
Hafeez Ud Din ◽  
Rabia Ahmed
Keyword(s):  
Colorectal Carcinoma ◽  
Tumor Cells ◽  
Tumor Infiltrating Lymphocytes ◽  
Armed Forces ◽  
Cross Sectional Study ◽  
Epithelial Tumor ◽  
Cross Sectional ◽  
Morphological Evaluation ◽  
Level 3 ◽  
Infiltrating Lymphocytes

Objective: To conduct a morphological evaluation quantitatively of two types of tumor-infiltrating lymphocyte populations, including those located in the stroma and intraepithelial cancer structures, in patients with colorectal cancer. Study Design: Cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology, Rawalpindi, from Jan to Jul 2019. Methodology: Three levels of infiltration in the stroma by tumor infiltrating lymphocytes were determined. Level 1 was weak meaning (0-20% of stromal TILs), level 2 was moderate meaning (>20-50% of stromal TILs); and level 3 was strong meaning (50-90% of stromal TILs). TILs within tumor cells were divided into two groups. 0 meaning absent (no TILs present) and 1 meaning present (≥TILs in tumor cells) 1. Results: Out of 30 cases 22 were males and 8 females. Ages ranged between 25-83 with a mean of 57 years and a standard deviation of ± 16.4 years. All of the cases were diagnosed cases of adenocarcinoma. The levels of stromal tumor infiltrating lymphocytes was weak in 1 case, moderate in 18 cases and strong in 11 cases whereas intraepithelial lymphocytosis was seen in 22 cases. Conclusion: These results confirm that the infiltration of tumor infiltrating lymphocytes into the tumor in patients with colorectal carcinoma serves an important role in the invasion and progression of the disease, and should be considered in routine examinations.

scholarly journals High expression of E-cadherin and Ki-67 associated with functional/dysfunctional phenotypes of tumor-infiltrating lymphocytes among Chinese patients with operable breast cancer

2018 ◽  
Vol 46 (12) ◽  
pp. 5219-5227 ◽  
Author(s):  
Ruibin Wang ◽  
Feng Shi ◽  
Lin Zhao ◽  
Yanjie Zhao ◽  
Guangjiang Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Infiltrating Lymphocytes ◽  
Cross Sectional Study ◽  
Clinicopathological Features ◽  
Chinese Patients ◽  
Ki 67 ◽  
Cross Sectional ◽  
Cell Counts ◽  
Infiltrating Lymphocytes ◽  
E Cadherin

Objective Breast cancer has become the most common cancer in women in China, and the clinicopathological features differ from those in Western patients. This study was performed to investigate the distribution of programmed cell death protein 1 (PD-1)+/PD-1− tumor-infiltrating lymphocytes (TILs) and its association with clinicopathological features among Chinese patients with breast cancer. Methods In total, 133 consecutive patients with primary breast cancer were recruited into this cross-sectional study from 2012 to 2013. TILs were measured by cell counts under high-power fields (HPFs). Immunohistochemistry was used to detect PD-1 expression on tumor-infiltrating lymphocytes in the microenvironment. Results The median cell counts of the overall TILs, PD-1+ TILs, and PD-1− TILs were 80, 18, and 55/HPF, respectively. The number of PD-1− TILs was significantly lower in older than younger patients (50 vs. 60/HPF). Patients with positive E-cadherin expression had more PD-1− TILs than patients with negative E-cadherin expression (57 vs. 27/HPF). The Ki-67 index was positively correlated with the cell counts of PD-1+ TILs, and the correlation coefficient was 0.29. Conclusions PD-1 expression on TILs had different clinicopathological features in Chinese patients with breast cancer. E-Cadherin expression was associated with PD-1− TILs; however, Ki-67 expression was associated with PD-1+ TILs.

Addition of immunotherapy in adjuvant setting could be a promising avenue of colorectal cancer (CRC) treatment, especially in patients with higher levels of programmed cell death ligand-1 (PD-L1) positive circulating epithelial tumor cells (CETC) after neoadjuvant chemotherapy.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e14036-e14036
Author(s):  
Monika Pizon ◽  
Dorothea Schott ◽  
Ulrich A. Pachmann ◽  
Katharina Pachmann ◽  
Siddarth Agrawal ◽  
...  
Keyword(s):  
Neoadjuvant Chemotherapy ◽  
Tumor Cells ◽  
Tumor Infiltrating Lymphocytes ◽  
Histological Subtypes ◽  
Epithelial Tumor ◽  
Clinicopathologic Factors ◽  
Infiltrating Lymphocytes ◽  
The Impact ◽  
Promising Avenue

e14036 Background: CRC is the third most frequent cancer in men and second in women worldwide. Recently, immune checkpoint molecules such as PD-1/PD-L1 have been identified as a possible target for immunotherapy in CRC and are increasingly being tested in clinical trials in combination with chemotherapy. However, the role of PD-L1 expression in CRC tumor cells and its interaction with other clinicopathologic factors remains elusive. In this study, we evaluated the impact of neoadjuvant chemotherapy on the fraction of PD-L1 positive CETCs and to compare its expression to the primary tumor. Methods: CETCs were determined from the blood of 20 patients suffering from CRC in different stages of the disease. The number of CETCs and their expression of PD-L1 were investigated using the maintrac method. In parallel, PD-L1 expression and tumor infiltrating lymphocytes (TILs) were assessed by immunohistochemistry on tissue biopsies. Results: PD-L1 expression could be assessed in all patients with detectable CETCs with a median of 50%. CETCs were more frequently found to be PD-L1 positive than tissue, and no correlation was observed between tissue and CETCs PD-L1 expression. Interestingly, patients with neoadjuvant chemotherapy had more positive PD-L1 CETCs compared to patients without chemotherapy (mean 66,7 vs. 35,2 p < 0.05), but the total number of CETCs was significantly lower in the neoadjuvant group. Also, patients with mucinous adenocarcinoma had more PD-L1 positive CETCs compared to other histological subtypes. The number of TILs was higher in patients with lymph node metastasis and larger tumors. TILs count did not differ in patients with and without neoadjuvant chemotherapy. Conclusions: Assessment of PD-L1 expression in CETCs is feasible, and CETCs are more often positive than in tissue. PD-L1 expression on CETCs was higher in patients who received neoadjuvant chemotherapy. Further studies are necessary to validate these findings.

scholarly journals The THE FREQUENCY OF MISMATCH REPAIR DEFICIENCY IN COLORECTAL CARCINOMA DETERMINED BY IMMUNOHISTOCHEMISTRY

2021 ◽  
Vol 71 (4) ◽  
pp. 1395-99
Author(s):  
Maria Naseem ◽  
Muhammad Asif ◽  
Hafeez Ud Din ◽  
Muhammad Tahir Khadim ◽  
Ahmed Ahson Khan ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Mismatch Repair ◽  
Armed Forces ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Mismatch Repair Deficiency ◽  
Cross Sectional ◽  
Repair Deficiency ◽  
The Mean ◽  
Mmr Proteins

Objective: To determine the frequency of mismatch repair deficiency in colorectal carcinoma determined by immunohistochemistry. Study Design: Cross-sectional study. Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi, from Aug 2018 to Jan 2019. Methodology: A total of 101 patients of adenocarcinoma of colorectum who underwent surgical resections and their characteristic and clinical data were recorded. Immunohistochemical stains were performed using antibodies MLH1, MSH2, PMS2 and MSH6. Results were interpreted and mismatch repair deficiency status of all patients was determined. Patients with loss of expression for MLH1, MSH2, PMS2 and MSH6 antibodies were observed and noted. Results: In this study, out of 101 patients with CRC, 71 (70.3%) were male and 30 (29.7%) female. The mean age was (54 years ± 15.9). Amongst the 101 cases loss of immunohistochemical staining for MMR proteins was noted in 19 patients (18.8%). The combined loss of all four antibodies was seen in one case, loss of MLH1 and PMS2 in 7, MSH2 and MSH6 in 5 and MLH1 only in 6 patients. However, no mismatch repair deficiency was detected in remaining 82 cases. According to statistical analysis no significant association between mismatch repair deficiency and variables was found. Conclusion: The frequency of mismatch repair deficiency in colorectal carcinoma patients was found to be 18.8% in our population.

scholarly journals Correlating tumor-infiltrating lymphocytes and lung cancer stem cells: a cross-sectional study

2019 ◽  
Vol 7 (22) ◽  
pp. 619-619 ◽  
Author(s):  
Valentina Masciale ◽  
Giulia Grisendi ◽  
Federico Banchelli ◽  
Roberto D’Amico ◽  
Antonino Maiorana ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Tumor Infiltrating Lymphocytes ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Lung Cancer Stem Cells ◽  
Infiltrating Lymphocytes

scholarly journals Discharge against medical advice from the emergency department in a university hospital

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Feras H. Abuzeyad ◽  
Moonis Farooq ◽  
Salah Farhat Alam ◽  
Mudhaffar Ismael Ibrahim ◽  
Luma Bashmi ◽  
...  
Keyword(s):  
Emergency Department ◽  
Cross Sectional Study ◽  
Small Sample ◽  
Medical Advice ◽  
University Hospital ◽  
High Risk Population ◽  
Cross Sectional ◽  
Against Medical Advice ◽  
Level 3 ◽  
Collection Form

Abstract Background Patients discharged against medical advice (DAMA) act as a high-risk population for the Emergency Department (ED), regardless of their presentations, and can pose a serious burden for the hospital. This study examines the prevalence, demographic and clinical characteristics, reasons, and clinical outcomes of a small sample of DAMA patients in a teaching university hospital, including readmission, morbidity, and mortality. Methods A prospective, descriptive cross-sectional study was conducted in the ED of King Hamad University Hospital (KHUH) with 98,992 patient visits during a 1-year period from June 2018 to June 2019. Consenting DAMA patients were asked to complete a data collection form. Results Patients (n = 413) had a mean age of 44.1 years with a female majority (57.1%). The majority were categorized as triage level-3 (87.7%). The main reasons for DAMA included refusal of the procedure/operation (23.2%), long ED waiting time (22.2%), subjective improvement with treatment (17.7%), and children at home (14.8%), whereas the least selected reason was dissatisfaction with medical care (1.2%). Follow-up of DAMA patients revealed that 86 cases (20.8%) were readmitted to the ED within 72 h of which 41 (47.7%) cases were morbidity and 2 (2.3%) were mortality. Marital status was a predictor of DAMA patients who revisit the ED within 72 h. Conclusion The results act as a pilot study to examine a small sample of DAMA patients’ characteristics, diagnosis, and ED revisits. Hospitals should investigate further the DAMA population on a larger scale, reasons for refusing procedures, and utilize this knowledge to improve the healthcare process.

Prevalence of Colorectal Carcinoma With Respect to Age and Gender

2021 ◽  
Vol 15 (12) ◽  
pp. 3198-3199
Author(s):  
Pervez Muhammad ◽  
Sajjad Muhammad ◽  
Muhammad Tariq ◽  
Amir Khan ◽  
Akhtar Munir ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Colorectal Adenocarcinoma ◽  
Cross Sectional Study ◽  
Medical Institute ◽  
Cross Sectional ◽  
Age And Gender ◽  
Medical College ◽  
Gender Study ◽  
The Mean ◽  
And Gender

Aim: Prevalence of colorectal carcinoma with respect to age and gender Study design: Observational / cross sectional study. Place and duration of study: This study was conducted at the Department of Pathology and Surgery, Post Graduate Medical Institute Lahore and Jinnah Medical College Peshawar from July, 2013 to February, 2015 Methodology: Surgical specimens of 60 histopathologically diagnosed cases of colorectal adenocarcinoma were included in this study. Age and sex were evaluated in CRC. Results: The mean±sd of age was 54.4±19.7 yrs. Out of 60 cases, 6 (10%) were between 16-29 yrs, 9 (15%) cases were between 30-49 yrs, 34 cases (56.7%) were of 50-69 yrs and 11 cases (18.33%) were of 70 yrs. Out of sixty cases, males were 33 (55%) and females were 27(45%). Conclusion: There are 34 out of 60(56.7%) patients were 50-69 years and 33 out of 60 (55%) cases were males i.e. males dominance. Keywords: Colorectal adenocarcinoma, immunohistochemistry, gender

scholarly journals Prevalence of Metabolic Syndrome among Border Guard Bangladesh Personnel

2020 ◽  
Vol 14 (2) ◽  
pp. 97-100
Author(s):  
Md Abdul Wahab ◽  
Md Mustafizur Rahman ◽  
Md Abdur Razzak ◽  
Md Habibur Rahman ◽  
Mimi Parvin ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
International Diabetes Federation ◽  
Armed Forces ◽  
Cross Sectional Study ◽  
Age Group ◽  
Metabolic Abnormalities ◽  
Sectional Study ◽  
Ethnic Population ◽  
Cross Sectional ◽  
Medical College

Introduction: Metabolic syndrome (MetS) is a multifaceted syndrome comprises a cluster of metabolic abnormalities including hypertension, central obesity, insulin resistance, dyslipidemia, and is strongly associated with developing diabetes mellitus and cardiovascular diseases. Prevalence of MetS differs in different ethnic population by using different definition. Objectives: To determine the prevalence of the MetS and its component risk factors among Border Guard Bangladesh (BGB) personnel working in Chattagram Hill Tract (CHT). Materials & Methods: This descriptive cross-sectional study was conducted from October 2016 to February 2018 among 1455 male BGB personnel aged between 40 to 59 years and working in CHT of Bangladesh. Study subjects were selected at BGB hospital, Guimara, Khagrachari. The new International Diabetes Federation (IDF) guidelines were used to identify MetS. Detail history, clinical examination, anthropometry and blood pressure (BP) were measured and fasting plasma glucose (FPG) and lipid profile were assessed. Results: Among the 1455 respondents’ BMI, waist circumference, systolic and diastolic BP were found more than normal in 34.1, 37.7, 16.4% and 12.3% respectively. Biochemical parameters including FPG, TC, TG, and LDL-C was raised in 28.7%, 49.8%, 52.2% and 37.7% of respondents respectively. HDL-C was found decreased in 26.9% respondents. Age wise prevalence of MetS for age group 40-44, 45-49, 50-54 and 55- 59 years were found 21.3%, 21.7%, 24.1% and 26.1% of the respondents respectively. The overall prevalence of MetS was found 22.4%. Conclusion: The prevalence of the MetS in BGB personnel was found 22.4%. Appropriate measure should be taken to lower the MetS cases. Journal of Armed Forces Medical College Bangladesh Vol.14 (2) 2018: 97-100

scholarly journals Lumbosacral transitional vertebra in military aviation candidates: A cross-sectional study

2021 ◽  
Vol 65 ◽  
pp. 29-32
Author(s):  
R Vaidya ◽  
M Bhatia
Keyword(s):  
Armed Forces ◽  
Cross Sectional Study ◽  
Common Finding ◽  
Small Subset ◽  
Lumbosacral Junction ◽  
Cross Sectional ◽  
Transitional Vertebra ◽  
Medical Evaluation ◽  
Study Population ◽  
Lumbosacral Transitional Vertebra

Introduction: Lumbosacral transitional vertebra (LSTV) is a common anomaly of the lumbosacral junction with a prevalence of 4–35.9% in various studies. Plain radiography of the spine in anteroposterior and lateral projections is done for the evaluation of the spine in candidates coming for medical evaluation for flying duties in the armed forces. Material and Methods: An observational study was conducted on the whole spine series of radiographs done at a medical selection establishment. The study population included candidates reporting to the establishment for medical examination to ascertain fitness for flying duties. In a small subset of this study population having LSTV, the Ferguson’s view was done to better delineate the lumbosacral junction. Results: The analysis revealed a total 148 cases of LSTV with a prevalence of 13.9%. Ferguson’s view, undertaken among 30 doubtful cases, confirmed the presence of LSTV in 27 cases. Type IIa was observed to be the most common pattern of LSTV followed by Type IIIb. As per the existing policy, 63.8% of candidates with LSTV were considered unfit for flying duties. Conclusion: Flying duties in the armed forces require the highest standard of physical fitness. LSTV is a very common finding during the evaluation of candidates and it is appropriate that the cases of LSTV be evaluated thoroughly. In doubtful cases of LSTV, the Ferguson’s view is a useful supplementary view as it clearly delineates the lumbosacral junction.

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