scholarly journals NGHIÊN CỨU TUYỂN CHỌN VÀ ĐỊNH LOẠI CHỦNG LỢI KHUẨN LÊN MEN LACTIC TỪ QUẢ SIM (Rhodomyrtus tomentosa)

2021 ◽  
Vol 7 (22) ◽  
Author(s):  
Lệ Thương Hoàng Thị ◽  
Thanh Vân Trần Thị ◽  
Sim Ma A
Keyword(s):  
Vitamin C ◽  
Lactobacillus Plantarum ◽  
Rhodomyrtus Tomentosa

Cây sim mọc tự nhiên ở hầu hết các thôn trên địa bàn xã Côn Lôn, ngoài các giống phổ biến trên địa bàn nghiên cứu thì Rhodomyrtus tomentosa hiếm gặp hơn. Ở địa phương, quả sim được sử dụng chủ yếu nhiều để ngâm rượu, ăn tươi bên cạnh các sản phẩm chế biến từ quả như mứt sim, mật sim, vang sim. Dịch ép quả sim có hàm lượng tanin, hàm lượng vitamin C cao, có ý nghĩa lớn trong kháng khuẩn. Sử dụng các phương pháp nghiên cứu đặc thù chúng tôi đã  phân lập được 5 chủng lactic kí hiệu từ L1-L5. Định danh chủng L5 bằng việc giải trình tự 16S rADN của chủng kết quả thu được Lactobacillus plantarum (L. plantarum) thuộc chi Lactiplantibacillus, đặt tên chủng là: L. plantarum L5.

scholarly journals Sifat Fisika dan Kimia Yogurt Sinbiotik Kering Beku dengan Variasi Sukrosa

2020 ◽  
Vol 3 (2) ◽  
pp. 114-122
Author(s):  
Amelia Ayu Kusumaningtyas ◽  
Catur Budi Handayani ◽  
Sri Hartati
Keyword(s):  
Vitamin C ◽  
Water Content ◽  
Lactobacillus Plantarum ◽  
Analysis Of Variance ◽  
Sucrose Concentration ◽  
Chemical Properties ◽  
Lactobacillus Bulgaricus ◽  
Milk Product ◽  
Fermentation Products ◽  
Freeze Dried

ABSTRACTYogurt is a fermented milk product made by adding lactic acid bacteria Streptococcus thermophillus and Lactobacillus bulgaricus. Sinbiotic yogurt is one of the fermentation products that is being developed and is made by adding Lactobacillus plantarum Dad 13 as a probiotic and Lactobacillus plantarum Dad 13 as a probiotic and purple sweet potato as a prebiotic. Freeze dried synbiotic yogurt is a product made with freeze dried. When dry, cryoprotectant microbial cells need to be added to survive. Cryoprotectant used is sucrose. The purpose of this study was to study the effect of sucrose composition as cryoprotectant on physical (rehydration time) and chemical (air content, total insoluble air solids, vitamin C content and protein content) freeze dry synbiotic yogurt. The experimental design used was a completely randomized design (CRD), with variations of sucrose 0%, 2.5%, 5% and 7.5%. The data obtained were analyzed using Analysis of Variance (ANOVA) with a significance level of 5%. The results of the analysis of physical characteristics (rehydration time) freeze dry synbiotic yogurt between 31.34-40.19 seconds, and the concentration of sucrose does not conflict with the rehydration time. The results of the analysis of chemical properties (water content and vitamin C) freeze dry synbiotic yogurt between 6.20-6.63% and 0.16-0.24%. Sucrose concentration had no significant effect on water content and vitamin C. The results of the analysis of chemical properties (total insoluble air and protein insoluble solids) yogurt contained 50% -50.62-62.95% and 7.66-16.26%. Sucrose concentration significantly affects total insoluble solids in air and protein.Keywords: chemical; freeze dried; physic; sucrose; synbiotic yogurt ABSTRAKYogurt adalah produk hasil fermentasi susu yang dibuat dengan menambahkan bakteri asam laktat Streptococcus thermophillus dan Lactobacillus bulgaricus. Yogurt sinbiotik merupakan salah satu produk hasil fermentasi yang sedang dikembangkan dan dibuat dengan menambahkan bakteri asam laktat (BAL) Lactobacillus plantarum Dad 13 sebagai probiotik dan ubi ungu sebagai prebiotik. Yogurt sinbiotik kering beku merupakan produk yang diproses dengan pengeringan beku. Pada saat pengeringan beku, perlu ditambahkan pelindung (cryoprotectant) supaya sel mikrobia dapat bertahan hidup. Cryoprotectant yang digunakan adalah sukrosa. Tujuan dari penelitian ini untuk mengetahui pengaruh konsentrasi sukrosa sebagai cryoprotectant terhadap sifat fisika (waktu rehidrasi) dan kimia (kadar air, total padatan tidak larut air, kadar vitamin C dan kadar protein) yogurt sinbiotik kering beku. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL), dengan variasi sukrosa 0 %, 2,5 %, 5% dan 7,5 %. Data yang diperoleh dianalisis menggunakan Analysis Of Variance (ANOVA) dengan tingkat signifikansi 5 %. Hasil dari analisis sifat fisika (waktu rehidrasi) yogurt sinbiotik kering beku berkisar antara 31,34-40,19 detik, dan konsentrasi sukrosa tidak berpengaruh nyata terhadap waktu rehidrasi. Hasil dari analisis sifat kimia (kadar air dan vitamin C) yogurt sinbiotik kering beku berkisar antara 6,20-6,63 % dan 0,16-0,24 %. Konsentrasi sukrosa tidak berpengaruh nyata terhadap kadar air dan vitamin C. Hasil dari analisis sifat kimia (total padatan tidak larut air dan protein) yogurt sinbiotik kering beku berkisar antara 50,95-62,95 % dan 7,66-16,26 %. Konsentrasi sukrosa berpengaruh nyata terhadap total padatan tidak larut air dan protein.Kata Kunci: fisika; kering beku; kimia; sukrosa; yogurt sinbiotik

scholarly journals The Effect of Lactobacillus plantarum CQPC02 on Fatigue and Biochemical Oxidation Levels in a Mouse Model of Physical Exhaustion

2021 ◽  
Vol 8 ◽  
Author(s):  
Ruokun Yi ◽  
Min Feng ◽  
Qiuping Chen ◽  
Xingyao Long ◽  
Kun-Young Park ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Lactic Acid ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Vitamin C ◽  
Lactobacillus Plantarum ◽  
Muscle Glycogen ◽  
Biochemical Oxidation ◽  
Dose Dependent

Chinese Sichuan pickle is a fermented food rich in microorganisms. Microorganisms have the potential to become an important new form of potent future therapeutic capable of treating human disease. Selecting vitamin C as a positive control, a lactic acid bacteria (Lactobacillus plantarum CQPC02, LP-CQPC02) isolated from Sichuan pickle was given to mice over 4 weeks to investigate the effect of CQPC02 on fatigue levels and biochemical oxidation phenomena in exercise-exhausted Institute of Cancer Research (ICR) mice. The fatigue model was established by forced swimming of mice, the levels of hepatic glycogen, skeletal muscle glycogen, lactic acid, blood urea nitrogen and free fatty acid were measured by physicochemical methods, serum serum creatine kinase (CK), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were measured by kits, the histopathological changes in the livers of mice were observed by H&E slicing, and the mRNA changes in the livers and skeletal muscles were observed by quantitative polymerase chain reaction (qPCR). Both vitamin C and LP-CQPC02 increased swimming exhaustion time. The concentration of LP-CQPC02 and exhaustion time were positively correlated. LP-CQPC02 also increased liver glycogen, skeletal muscle glycogen and free fatty acid content in mice and reduced lactic acid and blood urea nitrogen content in a dose-dependent manner. As walnut albumin antioxidant peptide concentration increased, levels of mouse CK, AST, and AST gradually decreased. LP-CQPC02 increased SOD and CAT levels and decreased MDA levels in a dose-dependent fashion. LP-CQPC02 up-regulated expression of mRNA encoding copper/zinc-superoxide dismutase (Cu/Zn-SOD), manganese-superoxide dismutase (Mn-SOD), and CAT in swimming exhaustion mouse liver tissue. LP-CQPC02 also up-regulated alanine/serine/cysteine/threonine transporter 1 (ASCT1) expression while down-regulating syncytin-1, inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-α) expression in swimming exhaustion mouse skeletal muscle. Overall, LP-CQPC02 had a clear anti-fatigue and anti-oxidation effect. This suggests that LP-CQPC02 can be developed as a microbiological therapeutic agent.

Striae Gravidarum, Vitamin C and Other Related Factors

2011 ◽  
Vol 81 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Rahime Bedir Findik ◽  
Nurcihan Karakurt Hascelik ◽  
Kadir Okhan Akin ◽  
Ayse Nurcan Unluer ◽  
Jale Karakaya
Keyword(s):  
Family History ◽  
Vitamin C ◽  
Pregnant Women ◽  
Smoking Status ◽  
Multiple Logistic Regression Analysis ◽  
Blood Levels ◽  
Significant Relation ◽  
Related Factors ◽  
Thigh Circumference ◽  
Striae Gravidarum

Background: Striae gravidarum, a clinical condition commonly seen in pregnant women, produces serious cosmetic problems and may lead to psychological problems. Aim: The present study investigated whether there was any relation between the presence of striae in primigravid pregnant women and blood vitamin C levels, and factors thought to contribute to the formation of striae such as family history, weight gained during pregnancy, smoking status, abdominal and thigh circumference, and age. Methods: Overall, 69 primigravid women attending routine antenatal follow-up and, using prophylactic iron and vitamin preparations, underwent investigation. All were pregnant 36 or more weeks. Scoring was based on striae examination and whether striae were present. The relation between the presence of striae, vitamin C blood levels, and other factors was investigated. Results and Conclusions: Multiple logistic regression analysis showed a significant relation between the presence of striae and blood vitamin C levels (p = 0.046) and between the presence of striae and family history (p = 0.023). No significant relation was found between the presence of striae and age, weight gained during pregnancy, abdominal and thigh circumference, or smoking status. It was concluded that further, more comprehensive studies on the issue are required.

The Relationship Between Antioxidant Nutrient Intake and Cataracts in Older People

2006 ◽  
Vol 76 (6) ◽  
pp. 359-366 ◽  
Author(s):  
Rodríguez-Rodríguez ◽  
Ortega ◽  
López-Sobaler ◽  
Aparicio ◽  
Bermejo ◽  
...  
Keyword(s):  
Vitamin C ◽  
Older People ◽  
Dietary Intake ◽  
Protective Effect ◽  
Elderly People ◽  
Nutrient Intake ◽  
Institutionalized Elderly ◽  
Weighing Method ◽  
The Relationship

This study investigated the relationship between the intake of antioxidant nutrients and the suffering of cataracts in 177 institutionalized elderly people (61 men and 116 women) aged ≥ 65 years. Dietary intake was monitored for 7 consecutive days using a "precise individual weighing" method. Subjects, who during their earlier years were exposed by their work to sunlight, had a greater risk of suffering cataracts (OR = 3.2; Cl: 1.1–9.3, P < 0.05) than those who worked indoors. A relationship was found between increased vitamin C intake and a reduced prevalence of cataracts (i.e., when comparing those above P95 for vitamin C intake with those below P5; (OR = 0.08; Cl: 0.01–0.75, P 0.05). Among subjects with cataracts, 12.1% had vitamin C intakes of < 61 mg/day (P10) and only 2.2% had intakes of > 183 mg/day (P95) (p < 0.01). Subjects who consumed > 3290 μg/day (P95) of lutein were less likely to have cataracts (OR = 0.086; Cl: 0.007–1.084; p < 0.05) than those whose consumption was < 256 μg/day (P5). In men, high intakes of zeaxanthin seemed to provide a protective effect against the problem (OR = 0.96; Cl: 0.91–0.99; p < 0.05). The results suggest an association exists between exposure to sunlight and the development of cataracts, and that vitamin C, lutein, and zeaxanthin offer some protection against this disorder.

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