Can worms be used to produce amendments with reduced CO<sub>2</sub> emissions during co-composting with clay and biochar and after their addition to soil?
Abstract. In this study we evaluated CO2 emissions during co-composting and co-vermicomposting of green wastes with clay and/or biochar. The stability of the final products as well as their effect on C mineralization in soil have been evaluated. The aim of the study was to test the following hypothesis: (1) interactions between clay and biochar and organic wastes would lead to reduced CO2 emissions during the composting process, (2) these interactions would be enhanced in the presence of worms, and (3) more carbon would be sequestered in soil after the use of the resulting compost/vermicompost as amendments. We added two different doses of clay, biochar and their mixture to pre-composted green wastes and monitored C mineralisation during 21 days in presence or absence of worms (Eisenia species). The organic materials were then added to a loamy Cambisol and the CO2 emissions were monitored during 30 days in a laboratory incubation. Our results indicated that the addition of clay or clay/biochar mixture reduced carbon mineralization during co-composting without worms by up to 44 %. However, in the presence of worms, CO2 emissions increased for all treatments except for the low clay dose. The production conditions had more influence on C mineralization in soil for composts than for vermicomposts except for the low clay treatment, which showed a more reduced CO2 emissions compared to a regular compost. In summary, the addition of worms during co-composting with clay and biochar may be a promising technology for reducing CO2 emissions and increasing soil carbon storage. We suggest that the production of a low CO2 emission amendment requires optimisation of OM source, co-composting agents and worm species. The effect of the resulting material on soil fertility has to be evaluated.