scholarly journals RAT BONE MARROW MESENCHYMAL STEM CELLS ISOLATION, CULTIVATION AND DIFFERENTIATION

2009 ◽  
Vol 15 (1) ◽  
pp. 30-34
Author(s):  
Emoke PALL ◽  
Ioan GROZA ◽  
Olga SORITAU ◽  
Ciprian TOMULEASA ◽  
Mihai CENARIU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Marrow Aspiration ◽  
Homogeneous Population ◽  
Adult Rat ◽  
Adult Mesenchymal Stem Cells ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

Bone marrow stromal cells (MSCs) represent a heterogeneous population derived from the non–blood-forming fraction of bone marrow that regulates hematopoietic cell development. In vitro, adult mesenchymal stem cells resident in this bone marrow fraction differentiate into bone, cartilage, and fat. Because MSCs can be easily obtained using a simple bone marrow aspiration and show extensive capacity for expansion in vitro, these cells have been considered as candidates for cell therapy. The aim of this study was to purify rat MSCs from adult bone marrow and to functionally characterise their abilities to differentiate along diverse lineages. Our data demonstrate that we successfully isolated, culture-expanded and differentiated a relatively homogeneous population of MPCs from adult rat bone marrow.

Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

2018 ◽  
Vol 18 ◽  
Author(s):  
Chaitra Venugopal ◽  
Christopher Shamir ◽  
Sivapriya Senthilkumar ◽  
Janitri Venkatachala Babu ◽  
Peedikayil Kurien Sonu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuroprotective Effects ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Rat Bone

scholarly journals Dihydrotestosterone and 17-Estradiol Enhancement of In Vitro Osteogenic Differentiation of Castrated Male Rat Bone Marrow Mesenchymal Stem Cells (rBMMSCs)

2019 ◽  
Author(s):  
FAM Abo-Aziza ◽  
AA Zaki ◽  
AS Amer ◽  
RA Lotfy
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Population Doubling ◽  
Calcium Deposition ◽  
Alp Activity ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

Background: In vitro impact of dihydrotestosterone (DHT) and 17-estradiol (E2) in osteogenic differentiation of castrated rat bone marrow mesenchymal stem cells (rBMMSC) still need to be clarified. Materials and Methods: The viability, proliferation and density of cultured rBMMSC isolated from sham operated (Sham) and castrated (Cast) male rats were evaluated. rBMMSC were cultured with osteogenic differentiating medium (ODM) in the presence of DHT (5,10 nM) and E2 (10,100 nM). Osteogenesis was evaluated by alizarin red staining and measurement of calcium deposition and bone alkaline phosphatase (BALP) activity. Results: Population doubling (PD) of rBMMSC isolated from Cast rats was significantly lower (P<0.05) compared to that isolated from Sham rats. rBMMSC from Cast rats showed low scattered calcified nodule after culturing in ODM and did not cause a significant increase in calcium deposition and B-ALP activity compared to rBMMSCs from Sham rats. Exposure of rBMMSC isolated from Cast rats to DHT (5 nM) or E2 (10 nM) in ODM showed medium scattered calcified nodules with significantly higher (P<0.05) calcium deposition and B-ALP activity. Moreover, exposure of rBMMSC to DHT (10 nM) or E2 (100 nM) showed high scattered calcified nodules with higher (P<0.01) calcium deposition and B-ALP activity Conclusion: These results indicated that the presence of testes might participate in controlling the in vitro proliferation and osteogenic differentiation capacity of rBMMSCs. DHT and E2 can enhance the osteogenic capacity of rBMMSCs in a dose-dependent manner. Based on these observations, optimum usage of DHT and E2 can overcome the limitations of MSCs and advance the therapeutic bone regeneration potential in the future.

In Vitro Cultivation Technology of Rat Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 9 (1) ◽  
pp. 62-68
Author(s):  
Wei Li ◽  
Junjie Zeng ◽  
Ganghua Zhu ◽  
Yunpeng Dong ◽  
Dinghua Xie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
In Vitro Cultivation ◽  
Marrow Mesenchymal Stem Cells ◽  
Cultivation Technology ◽  
Rat Bone

Self-organized 3D mineralized bone-like structures developed in vitro from rat bone marrow mesenchymal stem cells (MSCs)

Bone ◽  
2006 ◽  
Vol 38 (5) ◽  
pp. S2
Author(s):  
V. Francalancia ◽  
A. Lewis ◽  
A. Chertcoff ◽  
M.A. Da Silva Minas ◽  
A. Cole ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Self Organized ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

scholarly journals Increased Activity Of Mature Osteoblast from Rat Bone Marrow-Mesenchymal Stem Cells tn Osteogenic Medium Exposed to Melatonin

2018 ◽  
Vol 54 (4) ◽  
pp. 282
Author(s):  
Yugi Hari Chandra Purnama ◽  
Gondo Mastutik ◽  
Suhartono Taat Putra
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Optimal Dose ◽  
Osteogenic Medium ◽  
Osteoblast Activity ◽  
Marrow Mesenchymal Stem Cells ◽  
Physiological Dose ◽  
Rat Bone

Exposure to melatonin in the cultures of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) in osteogenic medium is able to induce mesenchymal stem cells and preosteoblasts into active osteoblasts via several transduction signals such as ERK 1/2. Previous studies used a single dose of 50 nM and a physiological dose of 20-200 pg/ml. The objective of the study was to obtain an optimal dose of melatonin that enhances osteoblast activity by increasing the expression of ERK1/2 and ALP levels in the culture of Rat Bone Marrow Mesenchymal Stem Cells (BM-MSCs) in osteogenic medium. This study was an in vitro experimental laboratory study using BM-MSCs from rat femoral bone grown on osteogenic medium without or with exposure to melatonin in doses of 0, 50, 100, 150 nM for 21 days. BM-MSCs were characterized by immunocytochemical techniques (CD45- and CD 105+) and ERK 1/2 expression was checked 24 hours after exposure to melatonin, while ALP levels were examined on day 21 using ELISA technique. ERK 1/2 expression on BM-MScs exposed to melatonin in doses 0, 50, 100, and 150 nM were respectively 0.087, 0.095, 0.081, and 0.079. Mean ERK 1/2 expression in various groups showed a decrease along with increasing doses of melatonin. Among the four treatment groups, the administration of melatonin in a dose of 50 nM resulted in highest mean ERK 1/2 expression. ALP levels in BM-MSCs exposed to melatonin doses of 0, 50, 100, and 150 nM were 0.128; 0.130; 0.117, and 0.111 ng/ml respectively. Data showed that decreasing mean ALP levels occurred along with the addition of melatonin dose. In conclusion, the administration of melatonin 50 nM is the optimal dose to increase the differentiation of cultured rat BM-MSCs into active osteoblasts.

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