The Anti-Diabetic and Anti-Hyperlipidemia effect of Citrus maxima Fruit Peel extract in Streptozotocin - Induced Diabetic Rats

2021 ◽  
pp. 5355-5358
Author(s):  
Shaimaa M. Mohammed ◽  
Afnan E. Abd-Almonuim ◽  
Ahmed Majeed ◽  
Haithm Khlaf
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Fruit Peel ◽  
Blood Samples ◽  
Citrus Maxima

The ethanol extract of Citrus maxima fruit peel was evaluated for its hypoglycaemic and hypolipidemic activity in normal and Streptozotocin (STZ)-induced diabetic rats, using fasting and glucose tolerance test measurements. Experiments were performed using Thirty-Two Male Wister albino rats randomly divided into 4 groups and each group have 8 animals. Group1 assigned as a control injected with normal saline only. Group 2 assigned as a diabetic control injected with Streptozotocin 50mg/Kg, Group 3 assigned for diabetic + Citrus maxima in a dose of 400mg/Kg, Group 4 is assigned for the diabetic + Citrus maxima in a dose of 600mg/Kg. The Streptozotocin is injected intraperitonially to all animal in the groups except the control group. Blood samples were collected from animal before and at 21th day end of the study period. Body weight, blood glucose, serum total cholesterol (TC), triglyceride (TG), and HDL cholesterol were analysed using diagnostic kits. Serum was separated from blood samples collected. In addition oral glucose tolerance test was performed in overnight fasted control animals. Results showed that Citrus maxima extract possesses significant antidiabetic activity against streptozotocin induced diabetic rats by decreasing blood glucose levels, maintaining body weight, and serum lipid concentrations to approximate normal level. Furthermore, the extract of the title plant possesses dose dependent antidiabetic activity.

scholarly journals Effects of malathion exposure on glucose tolerance test in diabetic rats; emphasis on oxidative stress and blood concentration of malathion by gas chromatography mass spectrometry

2021 ◽  
Vol 4 (02) ◽  
pp. 60-71
Author(s):  
Seyedeh-Azam Hosseini ◽  
Ali Faghihi zarandi ◽  
Somayyeh Karami-Mohajeri
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Gas Chromatography Mass Spectrometry ◽  
Blood Samples

Malathion is one of the widely used broad-spectrum organophosphate insecticides (OPI) in Iran. Malathion affects carbohydrate metabolism, causes hyperglycemia and increases the risk of diabetes. The present study was undertaken to investigate the potential of malathion to exacerbate diabetes-induced oxidative stress and impairment in blood glucose level and glucose tolerance in a sub-acute study. Malathion concentration in blood analyzed with gas chromatography mass spectrometry (GC-MS) after sample preparation of blood samples based on magnetic Fe3O4-supported graphene oxide (Fe3O4@ GO) nanoparticles. Type 1 diabetes was experimentally induced by intraperitoneal administration of streptozocin (65 mg kg-1). Diabetic and non-diabetic rats were treated with malathion at the dose of 150 mg kg-1day-1 or 0.5-4.0 mg L-1 in blood for 4 weeks. Fasting blood glucose was measured every week. At the end of the study, blood samples were investigated for markers of oxidative stress. Exposure to multiple doses of malathion decreased the total antioxidant capacity of plasma and the activity of catalase and superoxide dismutase enzymes in diabetic rats. Blood glucose and glucose tolerance test (GTT) and oxidative damages did not change significantly in diabetic rats exposed to malathion. However, malathion concentration in blood caused to increase GTT in malathion-treated non-diabetic rats. 

Antidiabetic Effects of Ethanolic Extract of Ficus glomerata (L.) Roots

2020 ◽  
Vol 16 (1) ◽  
pp. 33-41
Author(s):  
Mohini C. Upadhye ◽  
Uday Deokate ◽  
Rohini Pujari ◽  
Vishnu Thakare
Keyword(s):  
Body Weight ◽  
Glucose Tolerance ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Oral Glucose ◽  
Ficus Glomerata

Background: Ficus glomerata (F. glomerata) Linn. Family Moraceace is a large tree found all over India including outer Himalayan ranges, Punjab, Chota Nagpur, Bihar, Orissa, West Bengal, Rajasthan, Deccan and also as a common plant in South India. It is planted around the home and temples. It is cultivated throughout the year, distributed in evergreen forests and moist localities. Objective: The Ethanolic Extract of roots of F. Glomerata (EEFG) belonging to the family Moraceace, was investigated for its antidiabetic activity using alloxan induced diabetic rats. Methods: Thirty rats were divided into 5 groups having 6 rats in each group. The alloxan was administered to the rats of all groups except normal control group through intraperitoneal route at a concentration of 140mg/kg body weight. A dose of 100mg/kg and 200 mg/kg body weight of EEFG was administered to alloxan induced diabetic rats. The administration of the extract was lasted for 11 days. Effectiveness of the extract on glucose, cholesterol, triglycerides, and high density lipoprotein and protein concentrations was analyzed. Results: Significant (p<0.05) reduction in the levels of glucose, cholesterol, triglyceride of the diabetic rats was observed after treatment with ethanolic extract. After subjecting to oral glucose tolerance test EEFG also showed significant improvement in glucose tolerance. Conclusion: F. glomerata root ethanolic extract showed that it possesses antidiabetic effect and can be found useful for the management of diabetes mellitus.

scholarly journals Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

2018 ◽  
Vol 5 (7) ◽  
pp. 2440-2454
Author(s):  
D. A. Omoboyowa ◽  
F. O. Afolabi ◽  
T. C. Aribigbola
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Methanol Extract ◽  
Stem Bark ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

scholarly journals Antidiabetic Potential of Mangifera indica L. cv. Anwar Ratol Leaves: Medicinal Application of Food Wastes

Medicina ◽  
2019 ◽  
Vol 55 (7) ◽  
pp. 353 ◽  
Author(s):  
Mohammad Saleem ◽  
Muiz Tanvir ◽  
Muhammad Furqan Akhtar ◽  
Mazhar Iqbal ◽  
Ammara Saleem
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Leaf Extract ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Mice ◽  
Mango Leaves ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Background and objectives: Anwar Ratol is one of the most famous cultivar of mango in South Asia, especially Pakistan. Mango leaves are left as food waste. This study evaluated the potential of mango (Anwar Ratol) leaves for their use against diabetes mellitus. Material and Methods: In this study, hydro-alcoholic extract of the plant leaves was prepared and evaluated by electrospray ionization mass spectroscopy (ESI-MS) and high-performance liquid chromatography (HPLC) for the presence of phytochemicals. The plant extract was administered to Alloxan induced diabetic mice followed by evaluation through oral glucose tolerance test; determination of postprandial glucose, body weight, lipid profile and histopathological evaluation of pancreas. Results: Chemical evaluation revealed the presence of mangiferin, rhamnetin, catechin, epicatechin, iriflophenone 3-C-β-D-glucoside, gallic acid and other phenolic and flavonoid compounds. The plant extract exhibited a decrease in postprandial blood glucose following seven days therapy in diabetic mice. The extract also prevented the rise in blood glucose level as determined by glucose tolerance test in diabetic mice. Furthermore, therapy of diabetic mice with the extract prevented a decrease in body weight and decline in beta-cell mass associated with alloxan and improved lipid profile. Conclusion: The findings of the study clearly suggested that the leaf extract of the plant might possess anti-diabetic activity possibly due to the presence of mangiferin and other phytochemicals such as phenolic and flavonoid compounds. This study will serve as a basis for the use of mango leaf extract against diabetes. Furthermore, this study will also provide basis for the bioassay-based fractionation and isolation of active principles responsible for the antidiabetic potential of mango leaves.

scholarly journals Effect of Burdock Root and the Fermented Product on Alloxan-Induced Mouse Hyperglycemia

2015 ◽  
Vol 4 (4) ◽  
pp. 10 ◽  
Author(s):  
Wakana Doi ◽  
Yumi Asada ◽  
Ayaka Ohno ◽  
Yoshiko Okuda ◽  
Shota Masuda ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Control Diet ◽  
Lipid Peroxide ◽  
Tolerance Test ◽  
C Peptide ◽  
Glucose Assay ◽  
Fermented Product

<p><strong>Backgrounds:</strong> We reported that feeding 5% <em>Asperagillus awamori-</em>fermented burdock root diet was effective in preventing mouse hyperglycemia caused by alloxan.</p> <p><strong>Methods:</strong> Diets containing 5% burdock roots were prepared from raw and <em>Asperagillus awamori-</em>fermented burdock root powders. Acatalasemic mice<strong>,</strong> having a quite low catalase activity in blood, and normal mice were fed these diets for 14 weeks, separately. Then, alloxan (200 mg/ kg of body weight) or PBS was intraperitoneally administrated to each mouse. After 5 day from the administration, blood glucose assay and glucose tolerance test were carried out, and then insulin, C-peptide and lipid peroxide in plasma were examined.</p> <p><strong>Results:</strong> Incidences of hyperglycemia in normal mice fed control, raw and fermented burdock root diets were 25, 20 and 11 %, respectively, and these in acatalasemic mice<strong> </strong>were 73, 80 and 27%. Insulin and C-peptide in plasma of mice fed raw burdock root diet or control diet were low compared to mice fed the fermented diet.</p> <p><strong>Conclusions:</strong> Intake of raw burdock root does not suppress the alloxan-induced hyperglycemia but the fermented burdock root does. It is suggested that <em>Asperagillus awamori</em> plays an important role for the prevention.</p>

scholarly journals Effect of Afghan Talkhak (Acroptilon Repens) Aqueous Extract on Blood Glucose Level in Diabetic Rats

2021 ◽  
Vol 12 (2) ◽  
pp. 275-279
Author(s):  
Banafsha Zainal Bayat ◽  
Kawsar Alami ◽  
Sayed Yousof Mousavi
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Aqueous Extract ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Acroptilon Repens

The aim of this study is the evaluation of the effect of Afghan talkhak (Acroptilon Repens) root aqueous extract on blood glucose level of diabetic rats. Rats were randomly divided into 5 groups (1 Normal, 1 Diabetic and 3 extract groups). Experimental diabetes was induced by single-dose intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg) in diabetic and extracts groups. 72 hour later, the induction of diabetes was approved and extract groups were received Acroptilon Repens aqueous extract (200, 300, 400 mg/kg) by i.p. administration for 15 days. The blood glucose level and body weight of all groups were measured on baseline, 0, 5, 10 and 15th days. The glucose tolerance test had performed on days 0 and 15. The results showed that aqueous extract of Afghan Acroptilon Repens in extract groups (200 and 300 mg/kg) could significantly decrease the blood glucose level, and improve the glucose tolerance, as compared with the diabetic group (P<0.0001). However, there was not a significant difference in the blood glucose level and glucose tolerance test among the group which received 400 mg/kg dose of Acroptilon Repens and the diabetic group (P>0.05). In conclusion, Acroptilon Repens root aqueous extract possessed antihyperglycemic activity. 

scholarly journals SCREENING & EVALUATION OF ANTI-DIABETIC SPECTRUM OF WITHANIA COAGULANS

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Mayank Bansal ◽  
Sweta Kumari ◽  
Yogesh Kumar Sharma
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Level ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Effect ◽  
Withania Coagulans

Diabetes mellitus is a metabolic disorder. This review article is planned to The anti-diabetic effect of Withania Coagulans have been successfully used in Ayurvedic and other traditional formulations and found to be efficient and inexpensive as compared to synthetic drugs. It also aims to test and verify the W. coagulans fruits, showed significant inhibition on postprandial rise in hyperglycemia post-sucrose load in normoglycemic rats as well as streptozotocin-induced diabetic rats. which is comparable to the standard antidiabetic drug Glibenclamide for medical care, The present study defines the systematic evaluation and the role of minerals in glycemic potential of aqueous extract of Withania coagulans fruits in order to develop an effective and safe alternative treatment for diabetes mellitus. These studies conducted to determine the antidiabetic effect of Withania coagulans and pancreatic histological features in streptozotocin induced diabetic rat. Results: The study is based on results of lowering in blood glucose levels of normal, sub, mild diabetic rats assessed during fasting blood glucose, glucose tolerance test studies. The dose of 1000mg/kg was identified as the most effective dose, which reduce the blood glucose level maximum by 35.2% at 5 hours in normal rats during blood glucose studies. Glucose tolerance test studies is normal, sub and mild diabetic rats showed the maximum reduction of 16.7, 29.8 and 38.5% at 4hour respectively. Long term study incase of severely diabetic rats showed reduction fasting glucose level respectively after 30 days of treatment. The present study confirming antidiabetic effect of aqueous extract of Withania Coagulans, helps in identifying the role of trace minerals like Mg and Ca responsible for antidiabetic potential of this potent indigenous shrub. Conclusion: The present research has presented comprehensive details of antidiabetic plants used in the treatment of diabetes mellitus. Most of these plant derived medicines. Keywords: Anti-Diabetic effect, Screening & Evaluation, Streptozocin, Glibenclamide, Diabetic rats.

Antidiabetic Effect of Aqueous Corrigiola telephiifolia in Streptozotocin- Induced Diabetic Rats

2020 ◽  
Vol 10 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Morad Hebi ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Phytochemical Screening ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Corrigiola telephiifolia Pourr, is a perennial species, woody distributed throughout the north of Africa. This plant is used in traditional Mediterranean preparations and has many traditional uses especially treatment of diabetes. Aim/Methods: The current research was carried out to evaluate the antidiabetic effect of Aerial Parts of Aqueous Extract (APAE) of Corrigiola telephiifolia (C. telephiifolia) on both normal and streptozotocin (STZ)-induced diabetic rats treated at a dose of 5 mg/kg for fifteen days. Additionally, the histopathological changes in the liver, morphometric analysis, Oral Glucose Tolerance Test (OGTT) in normal rats and preliminary phytochemical screening for various components were realized. Results: Single oral administration of the APAE of C. telephiifolia (5mg/kg) showed no significant change in glycaemia of normal and STZ-induced diabetic rats. In contrast, repeated oral administration of C. telephiifolia reduced blood glucose levels from 4.11 ± 0.10 mmol/L to 3.16 ± 0.16 mmol/L (p<0.01) 15 days after administration in normal rats. Furthermore, blood glucose levels decreased from 17.84 ± 1.75mmol/L to 1.93 ± 0.33 mmol/L (p<0.0001) in STZ diabetic rats after fifteen days of treatment. According to the oral glucose tolerance test, C. telephiifolia (5 mg/kg) was shown to prevent significantly the increase in blood glucose levels in normal treated rats 30 min after glucose administration when compared to the control group. Also, the liver architecture of diabetic rats treated by C. telephiifolia was improved when compared with the liver architecture of untreated diabetic rats. Concerning the preliminary phytochemical screening of C. telephiifolia, several compounds have been found such as polyphenols, flavonoids, saponins, mucilage and terpenoids. Conclusion: The results show that the aqueous extract of C. telephiifolia possesses significant antihyperglycemic activity.

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

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