scholarly journals Generation of Transgenic Rice without Antibiotic Selection Marker through Agrobacterium-mediated Co-transformation System

2012 ◽  
Vol 22 (9) ◽  
pp. 1152-1158
Author(s):  
Soo-Kwon Park ◽  
Tack-Min Kwon ◽  
Jong-Hee Lee ◽  
Dong-Jin Shin ◽  
Woon-Ha Hwang ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Selection Marker ◽  
Transformation System ◽  
Antibiotic Selection

scholarly journals Enhancement of Ganoderic Acid Accumulation by Overexpression of an N-Terminally Truncated 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Gene in the Basidiomycete Ganoderma lucidum

2012 ◽  
Vol 78 (22) ◽  
pp. 7968-7976 ◽  
Author(s):  
Jun-Wei Xu ◽  
Yi-Ning Xu ◽  
Jian-Jiang Zhong
Keyword(s):  
Ganoderma Lucidum ◽  
Coenzyme A ◽  
Catalytic Domain ◽  
Selection Marker ◽  
Transformation System ◽  
Protein Subunit ◽  
Ganoderic Acid ◽  
Content Type ◽  
Ganoderic Acids ◽  
Coenzyme A Reductase

ABSTRACTGanoderic acids produced byGanoderma lucidum, a well-known traditional Chinese medicinal mushroom, exhibit antitumor and antimetastasis activities. Genetic modification ofG. lucidumis difficult but critical for the enhancement of cellular accumulation of ganoderic acids. In this study, a homologous genetic transformation system forG. lucidumwas developed for the first time using mutatedsdhB, encoding the iron-sulfur protein subunit of succinate dehydrogenase, as a selection marker. The truncatedG. lucidumgene encoding the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was overexpressed by using theAgrobacterium tumefaciens-mediated transformation system. The results showed that the mutatedsdhBsuccessfully conferred carboxin resistance upon transformation. Most of the integrated transfer DNA (T-DNA) appeared as a single copy in the genome. Moreover, deregulated constitutive overexpression of the HMGR gene led to a 2-fold increase in ganoderic acid content. It also increased the accumulation of intermediates (squalene and lanosterol) and the upregulation of downstream genes such as those of farnesyl pyrophosphate synthase, squalene synthase, and lanosterol synthase. This study demonstrates that transgenic basidiomyceteG. lucidumis a promising system to achieve metabolic engineering of the ganoderic acid pathway.

Development of an integrative transformation system for the opportunistic pathogenic yeastCandida lusitaniae usingURA3 as a selection marker

Yeast ◽  
2004 ◽  
Vol 21 (2) ◽  
pp. 95-106 ◽  
Author(s):  
Fabienne François ◽  
Florence Chapeland-Leclerc ◽  
Jean Villard ◽  
Thierry Noël
Keyword(s):  
Selection Marker ◽  
Transformation System ◽  
Integrative Transformation ◽  
Opportunistic Pathogenic

scholarly journals Integrative Transformation System for the Metabolic Engineering of the Sphingoid Base-Producing Yeast Pichia ciferrii

2003 ◽  
Vol 69 (2) ◽  
pp. 812-819 ◽  
Author(s):  
Jung-Hoon Bae ◽  
Jung-Hoon Sohn ◽  
Chang-Seo Park ◽  
Joon-Shick Rhee ◽  
Eui-Sung Choi
Keyword(s):  
Metabolic Engineering ◽  
De Novo ◽  
Integration Site ◽  
Fold Increase ◽  
Selection Marker ◽  
Serine Palmitoyltransferase ◽  
Transformation System ◽  
Wild Type ◽  
Gene Encoding ◽  
Integrative Transformation

ABSTRACT We have developed an integrative transformation system for metabolic engineering of the tetraacetyl phytosphingosine (TAPS)-secreting yeast Pichia ciferrii. The system uses (i) a mutagenized ribosomal protein L41 gene of P. ciferrii as a dominant selection marker that confer resistance to the antibiotic cycloheximide and (ii) a ribosomal DNA (rDNA) fragment of P. ciferrii as a target for multicopy gene integration into the chromosome. A locus within the nontranscribed region located between 5S and 26S rDNAs was selected as the integration site. A maximum frequency of integrative transformation of approximately 1,350 transformants/μg of DNA was observed. To improve the de novo synthesis of sphingolipid, the LCB2 gene, encoding a subunit of serine palmitoyltransferase, which catalyzes the first committed step of sphingolipid synthesis, was cloned from P. ciferrii and overexpressed under the control of the P. ciferrii glyceraldehyde-3-phosphate dehydrogenase promoter. After transformation of an LCB2 gene expression cassette, several transformants that contained approximately five to seven copies of transforming DNA in the chromosome and exhibited about 50-fold increase in LCB2 mRNA relative to the wild type were identified. These transformants were observed to produce approximately two times more TAPS than the wild type.

Corrigendum to ‘An antibiotic selection marker for schistosome transgenesis’ [Int. J. Parasitol. 42 (2012) 123–130]

2012 ◽  
Vol 42 (7) ◽  
pp. 707
Author(s):  
Gabriel Rinaldi ◽  
Sutas Suttiprapa ◽  
José F. Tort ◽  
Anne E. Folley ◽  
Danielle E. Skinner ◽  
...  
Keyword(s):  
Selection Marker ◽  
Antibiotic Selection

Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation

Planta ◽  
2006 ◽  
Vol 225 (4) ◽  
pp. 897-906 ◽  
Author(s):  
Paoyuan Hsiao ◽  
Sanjaya ◽  
Ruey-Chih Su ◽  
Jaime A. Teixeira da Silva ◽  
Ming-Tsair Chan
Keyword(s):  
Plant Transformation ◽  
Selection Marker ◽  
Tryptophan Synthase ◽  
Antibiotic Selection
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