DEVELOPMENT AND VALIDATION OF A CHEMOMETRICS ASSISTED SPECTROSCOPIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF GALLIC ACID, ELLAGIC ACID AND CURCUMIN IN POLYHERBAL ANTIDIABETIC FORMULATIONS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 67-73
Author(s):  
M. A Shah ◽  
◽  
H. U. Patel ◽  
H.A Raj
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Spectroscopic Method ◽  
Least Square ◽  
Data Matrix ◽  
Simultaneous Estimation ◽  
Chemometric Methods ◽  
Mixed Solutions ◽  
Validation Set ◽  
Development And Validation

Two chemometric methods, Inverse Least Square (ILS) and Classical Least Square (CLS), were applied for the simultaneous estimation of gallic acid, ellagic acid and curcumin in polyherbal antidiabetic formulation. Twenty mixed solutions were prepared for the chemometric calibration as training set and 10 mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at 20 different wavelengths, from 241 to 279 nm with the interval of 2 nm (Δλ= 2 nm). The developed calibrations were successfully tested for three antidiabetic polyherbal formulations for their gallic acid, ellagic acid and curcumin contents. Developed methods were validated and root mean square error of precision (RMSEP) was determined. Both chemometric methods in this study can be satisfactorily used for the quantitative analysis in polyherbal dosage forms. The chemometric calculations were performed by using the chemometrics toolbox with MATLAB R2015a software.

CHEMOMETRICS ASSISTED SPECTROSCOPIC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS DETERMINATION OF SOFOSBUVIR AND DACLATASVIR DIHYDROCHLORIDE IN TABLET FORMULATION

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (03) ◽  
pp. 37-46
Author(s):  
Sapna M Rathod ◽  
Paresh U Patel
Keyword(s):  
Method Development ◽  
Spectroscopic Method ◽  
Principal Component ◽  
Tablet Formulation ◽  
Partial Least Square ◽  
Least Square ◽  
Simultaneous Estimation ◽  
Mixed Solution ◽  
Chemometric Methods ◽  
Validation Set

Four chemometric methods, namely Classical Least Square (CLS), Inverse Least Square (ILS), Partial Least Square (PLS) and Principal Component Regression (PCR), were developed for the simultaneous estimation of sofosbuvir and daclatasvir dihydrochloride in tablet formulation. Full factorial design was used to construct calibration set as well as validation set. Twenty five mixed solutions were prepared for calibration set and sixteen mixed solution of drugs were prepared for validation set. The absorbance of all prepared solutions was measured in the range of 230 nm to 335 nm at 16 wavelength points at an interval of 7 nm. Linearity was observed in the range of 10 – 90 µg/mL for sofosbuvir and 4 - 20 µg/mL for daclatasvir dihydrochloride. The developed chemometric methods were validated in terms of precision and accuracy as per ICH guidelines. The developed methods can be applied for the routine quantitative analysis of formulation.

SIMULTANEOUS ESTIMATION OF TELMISARTAN, HYDROCHLOROTHIAZIDE AND AMLODIPINE BESYLATE IN TABLET PREPARATION BY CHEMOMETRIC ASSISTED SPECTROPHOTOMETRIC ANALYSIS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (07) ◽  
pp. 59-66
Author(s):  
A. S Ghule ◽  
T. Thomas ◽  
M. Joseph ◽  
K. S. Navya Sree ◽  
K. Bhat
Keyword(s):  
Spectrophotometric Analysis ◽  
Partial Least Square ◽  
Least Square ◽  
Partial Least Square Regression ◽  
Data Matrix ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Mixed Solutions ◽  
Validation Set ◽  
Tablet Preparation

A chemometric method was developed by application of Partial Least Square regression to for the simultaneous estimation of telmisartan, hydrochlorothiazide and amlodipine besylate in tablet preparations. Calibration set was prepared considering seven sets; each set with twenty-four mixed solutions and twenty-one ternary mixed solutions, were prepared as a validation set. The absorbance data matrix for training set was obtained by recording absorbance within wavelength range 220-320 nm at 2nm intervals. The developed method was validated according to ICH Q2 (R1) guidelines and results were reported. The developed and validated multivariate method was successfully tested for laboratory mixtures as well as commercial tablet formulation of telmisartan, hydrochlorothiazide and amlodipine besylate.

DEVELOPMENT AND VALIDATION OF CHEMOMETRIC-ASSISTED UV-SPECTROPHOTOMETRIC METHOD FOR EPIGALLOCATECHIN GALLATE AND CURCUMIN IN TABLET FORMULATION

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (04) ◽  
pp. 45-51
Author(s):  
N. C. Patel ◽  
A. P. Patel ◽  
J. K. Patel
Keyword(s):  
Epigallocatechin Gallate ◽  
Tablet Formulation ◽  
Partial Least Square ◽  
Least Square ◽  
Data Matrix ◽  
Simultaneous Estimation ◽  
Calibration Data ◽  
Mixed Solution ◽  
Validation Set ◽  
Development And Validation

A chemometric method, Partial Least Square, was applied for the simultaneous estimation of epigallocatechin gallate and curcumin in tablet formulation. Twenty five mixed sample solutions were prepared for chemometric calibration as training set and sixteen mixed solution for validation set using Full Factorial Design. The absorbance data matrix was obtained by measuring absorbance at 20 different wavelengths in the range of 220 to 410 nm (Δλ = 10 nm). The developed calibration data was used to test tablet formulation containing epigallocatechin gallate and curcumin. The developed methods were validated using RMSECV and RMSEP. The chemometric calculations were performed using Minitab 16.1.1 and Microsoft Excel 2010. The method is also more accurate and precise than conventional UV methods.

Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid

2014 ◽  
Vol 7 (1) ◽  
pp. 2350-2355
Author(s):  
Pratik S Mehta ◽  
Pratik R. Patel ◽  
Rajesh R Parmar ◽  
M M K Modasiya ◽  
Dushyant A Shah
Keyword(s):  
Spectroscopic Method ◽  
Spectral Interference ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Development And Validation ◽  
Crossing Point

A novel, simple, accurate, sensitive, precise and economical derivative spectroscopic method was developed and validated for the determination of cefadroxil and probenecid in synthetic mixture. First order derivative spectroscopy method was adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 4-36 μg/ml for cefadroxil and of 5-25 μg/ml of probenecid. The zero crossing point for cefadroxil and probenecid was 260 nm and 237.8 nm respectively in 0.1N HCl. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found.   

scholarly journals Development and validation of analytical method for Irbesartan and Atorvastatin by simultaneous equation spectroscopic method

2015 ◽  
Vol 1 (4) ◽  
pp. 194
Author(s):  
Paras B Virani ◽  
Rajanit Sojitra ◽  
Hasumati Raj ◽  
Vineet Jain
Keyword(s):  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Limit Of Determination ◽  
Relative Standard ◽  
Acceptance Range ◽  
High Concentrations ◽  
Development And Validation ◽  
Validation Of Analytical Method

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Irbesartan and atorvastatin in synthetic mixture using simultaneous equation Method. In this spectroscopic method, 226.00 nm and 246.00 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 05-30 ?g/ml at their respective ?max. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.033?g/ml and 0.125 ?g/ml for Irbesartan and atorvastatin, respectively. The limit of quantification was 0.1008 ?g/ml and 0.3792 ?g/ml for Irbesartan and atorvastatin, respectively. Recovery of Irbesartan and atorvastatin were found to be 99.75 % and 99.52% respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

SIMULTANEOUS ESTIMATION OF EMITRICITABINE, EFAVIRENZ, TENOFOVIR DISPROXIL FUMARATE BY CHEMOMETRY AND REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (06) ◽  
pp. 62-69
Author(s):  
G. Ramya Kumari ◽  
◽  
N. C Deepika ◽  
Krishnamurthy Bhat
Keyword(s):  
High Performance ◽  
Ultra Violet ◽  
Hplc Method ◽  
Tablet Formulation ◽  
Partial Least Square ◽  
Least Square ◽  
Ternary Mixtures ◽  
Data Matrix ◽  
Simultaneous Estimation ◽  
Tenofovir Df

A chemometric ultra-violet spectrophotometric method of analysis, Partial Least Square (PLS) method was applied to simultaneous assay of emitricitrabine, efavirenz and tenofovir disoproxil fumarate (DF) in their combined dosage tablet formulation. For comparison of this chemometric method a HPLC method for simultaneous determination of emitricitabine, efavirenz and tenofovir DF in combination was developed. Twenty one mixed solutions were prepared for chemometric calibration set and twenty one ternary mixtures were prepared as validation sets. The absorbance data matrix for training set was obtained by recording absorbance within wavelength range 230-290 nm at 2nm intervals. The developed multivariate and HPLC methods were successfully tested for laboratory mixtures as well as commercial tablet formulation of emitricitrabine, efavirenz, tenofovir DF.

DEVELOPMENT AND VALIDATION OF A SENSITIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PITAVASTATIN CALCIUM AND FENOFIBRATE

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 17-21
Author(s):  
A Kukrety ◽  
◽  
K. Kohli ◽  
M. Singhal ◽  
C Dhal ◽  
...  
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Standard Additions ◽  
Pitavastatin Calcium ◽  
Development And Validation ◽  
Different Levels ◽  
Sensitive Spectrophotometric Method

A simple, rapid, accurate, precise and reproducible UV spectroscopic method has been developed for the simultaneous estimation of pitavastatin calcium and fenofibrate in bulk and pharmaceutical dosage form. The method is based on simultaneous equation method. Pitavastatin calcium and fenofibrate have absorption maxima (λmax) at 245 nm and 286 nm respectively. Beer’s law was obeyed in the concentration range of 2-12 μg/mL and 32-192 μg/mL for pitavastatin calcium and fenofibrate, respectively. The recovery studies are indicative of accuracy of method and are found in between 97.66-107.22% and 103.33-110.55% for pitavastatin calcium and fenofibrate, respectively, at three different levels of standard additions. Precision studies showed satisfactory results.

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