scholarly journals Simple two spectrophotometric methods for estimation of Cephalexin in pure and pharmaceutical dosage form

2021 ◽  
Vol 3 (1) ◽  
pp. 37-45
Author(s):  
Neda Ibrahim Mahdi .
Keyword(s):  
Area Under Curve ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Zero Order ◽  
Maximum Absorption ◽  
Order Method ◽  
Pharmaceutical Dosage Form ◽  
Spectrophotometric Methods ◽  
Curve Method

This study describes two extraction-free and direct spectrophotometric methods for the determination of Cephalexin in a pure and pharmaceutical dosage form. In this study, a zero-order method has exhibited maximum absorption max at 263 nm, while area under curve method has calculated in the range 260-266nm. The Linearity of both methods in the range (10- 60 nm), the limit of detection (LOD) is 0.808 for Zero-order method and 0.781 for the area under curve method. These methods are successfully applied to determination of Cephalexin in a pharmaceutical dosage form.

UV SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF PALBOCICLIB IN BULK AND IN-HOUSE CAPSULE FORMULATION

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 35-40
Author(s):  
Rahul D. Rathod ◽  
Anand B. Mundada ◽  
Harun M. Patel ◽  
Atul A Shirkhedkar ◽  
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Zero Order ◽  
Distilled Water ◽  
Order Method ◽  
Capsule Formulation ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Made In

Four different simple, accurate and precise UV-spectrophotometric methods have been developed for the estimation of palbociclib (PB) in bulk and in-house capsule dosage form by zero order (Method I), zero order AUC (Method II), ‘first order derivative UV-spectrophotometric (Method III), and first order AUC (Method IV) methods. The drug was dissolved in methanol (AR-Grade) and further dilution was made in double distilled water. Zero order was performed at λ max 220.00 nm of PB (Method I) and AUC was calculated between 215.40 nm - 228.20 nm wavelength (Method II). In Method-III zero-order spectra were derivatized into first-order and amplitude measured at 231.00 nm and the AUC was recorded between 224.00 nm - 240.60 nm (Method IV). PB followed linearity in the concentration range of 4.08-20.40 µg/mL with correlation coefficient (r2 )> 0.99 for PB.

Method Development and Validation of Spectrophotometric Methods for The Estimation of Rizatriptan Benzoate in Pure and Pharmaceutical Dosage Form

2021 ◽  
pp. 6003-6006
Author(s):  
Pushpa Latha E. ◽  
Sailaja B.
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Pharmaceutical Dosage Form ◽  
Rizatriptan Benzoate ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage

Analytical UV derivative spectrophotometric method was developed and validated to quantify Rizatriptan Benzoate in pure drug and tablet dosage form. Based on the spectrophotometric characteristics of Rizatriptan Benzoate, a signal of zero (225nm), first (216nm), second (237nm), third (233nm), fourth (231nm) order derivative spectra were found to be adequate for quantification. The methods obeyed Beer's law in the concentration range of (0.1-360µg/ml) with square correlation coefficient (r2) of 0.999. The mean percentage recovery was found to be 100.01 ± 0.075. As per ICH guidelines the results of the analysis were validated in terms of linearity, precision, accuracy, limit of detection and limit of quantification, and were found to be satisfactory.

scholarly journals Zero order and area under curve spectrophotometric methods for determination of Levocetirizine in pharmaceutical formulation

2015 ◽  
Vol 1 (6) ◽  
pp. 270
Author(s):  
Audumbar Digambar Mali ◽  
Ritesh Bathe ◽  
Manojkumar Patil ◽  
Ashpak Tamboli
Keyword(s):  
Area Under The Curve ◽  
Zero Order ◽  
Pharmaceutical Dosage Forms ◽  
Mean Values ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Significant Difference ◽  
Curve Method ◽  
The Mean

Simple, fast and reliable spectrophotometric methods were developed for determination of Levocetirizine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in methanol. The quantitative determination of the drug was carried out using the zero order derivative values measured at 230 nm and the area under the curve method values measured at 227-234 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Levocetirizine using 5-25?g/ml (r=0.998 and r=0.999) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Levocetirizine in tablets.

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