scholarly journals Evaluation of Uric Acid, Total Antioxidant and Lipid Peroxidation Parameters in Serum and Saliva of Patients with Oral Lichen Planus

2016 ◽  
Vol 8 (12) ◽  
pp. 225 ◽  
Author(s):  
Atena Shiva ◽  
Shahin Arab
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Control Group ◽  
Healthy Controls ◽  
Total Antioxidant ◽  
Healthy Control ◽  
Oral Lichen ◽  
And Oxidative Stress

<p><strong>BACKGROUND</strong><strong>: </strong>Oral lichen planus (OLP) is a chronic and inflammation mucosal disease. Reactive Oxygen Species (ROS) and oxidative stress damage might be the cause. Malondialdehyd (MDA), one parameter of lipid peroxidation is appropriate for DNA damage in OLP disease.</p><p><strong>OBJECTIVE</strong><strong>: </strong>To evaluate antioxidants and oxidative stress parameters in patients with oral lichen planus (OLP) diseases and healthy control group with compare on serum and salivary samples.</p><p><strong>MATERIAL </strong><strong>&amp;</strong><strong> METHODS: </strong>The research population included 22 patients with OLP which recently diagnosed and 22 healthy controls matched for periodontal status. Total antioxidant activity (TAA), Malondialdehyde (MDA) or lipid peroxidation product and uric acid (UA) were evaluated in both serum and saliva. The t-tests were used for differences between the two groups in normal distributed variables and also Spearman’s rho correlation coefficient for assessing association between serum and salivary fluids.</p><p><strong>RESULTS</strong><strong>: </strong>TAA levels in OLP patients showed significant result and lower than healthy control group (p=0.39). Also, results in the saliva MDA concentration, was significantly higher in OLP patients than controls. In correlation test, inverse and significant correlation was observed between the MDA and UA values(r=0. 682, P=0.0001) and a significant correlation was found between serum TAA and UA values.</p><p><strong>CONCLUSION</strong><strong>: </strong>This study showed that OLP groups have higher cellular lipid peroxidation in compare to healthy controls and low level of TAA than controls. Patients with OLP are believed to be more at risk of antioxidant-oxidative stress imbalance.</p>

Evaluation of prolidase activity and oxidative stress in patients with oral lichen planus and oral lichenoid contact reactions

2015 ◽  
Vol 45 (4) ◽  
pp. 281-288 ◽  
Author(s):  
Şule Batu ◽  
Duygu Ofluoğlu ◽  
Sertan Ergun ◽  
Saman Warnakulasuriya ◽  
Ezel Uslu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Prolidase Activity ◽  
Oral Lichen ◽  
And Oxidative Stress

Salivary mir-27b Expression in Oral Lichen Planus Patients: A Series of Cases and a Narrative Review of Literature

2020 ◽  
Vol 19 (31) ◽  
pp. 2816-2823 ◽  
Author(s):  
Dario Di Stasio ◽  
Laura Mosca ◽  
Alberta Lucchese ◽  
Donatella Delle Cave ◽  
Hiromichi Kawasaki ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Inflammatory Diseases ◽  
Biological Fluids ◽  
Critical Role ◽  
Invasive Procedure ◽  
Control Group ◽  
Study Group ◽  
Immune Functions ◽  
Oral Lichen

Background: microRNAs play a critical role in auto-immunity, cell proliferation, differentiation and cell death. miRNAs are present in all biological fluids, and their expression is essential in maintaining regular immune functions and preventing autoimmunity, whereas miRNA dysregulation may be associated with the pathogenesis of autoimmune and inflammatory diseases. Oral lichen planus (OLP) is an inflammatory disease mediated by cytotoxic T cells attack against epithelial cells. The present study aims to perform a specific microRNA expression profile through the analysis of saliva in this disease. Methods: The study group was formed by five patients (mean age 62.8±1.98 years; 3 females/2 males) affected by oral lichen planus and control group by five healthy subjects (mean age 59.8 years±2.3; 3 females/ 2 males); using a low-density microarray analysis, we recorded a total of 98 differentially expressed miRNAs in the saliva of patients with oral lichen planus compared to the control group. The validation was performed for miR-27b with qRT-PCR in all saliva samples of oral lichen planus group. Results: 89 miRNAs were up-regulated and nine down-regulated. In details, levels of miR-21, miR- 125b, miR-203 and miR15b were increased (p<0.001) in study group while levels of miR-27b were about 3.0-fold decreased compared to controls (p<0.001) of miR-27b expression in OLP saliva. QRTPCR validation confirmed the down regulation of miR-27b in all saliva samples. Conclusions: Collecting saliva samples is a non-invasive procedure and is well accepted by all patients. microRNAs can be readily isolated and identified and can represent useful biomarkers of OLP.

scholarly journals NF-κB and FosB mediate inflammation and oxidative stress in the blast lung injury of rats exposed to shock waves

2021 ◽  
Author(s):  
Hong Wang ◽  
Wenjuan Zhang ◽  
Jinren Liu ◽  
Junhong Gao ◽  
Le Fang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lung Injury ◽  
Shock Waves ◽  
Time Dependent ◽  
Inflammatory Factors ◽  
Control Group ◽  
Dependent Manner ◽  
Total Antioxidant ◽  
Blast Lung Injury ◽  
And Oxidative Stress

Abstract Blast lung injury (BLI) is the major cause of death in explosion-derived shock waves; however, the mechanisms of BLI are not well understood. To identify the time-dependent manner of BLI, a model of lung injury of rats induced by shock waves was established by a fuel air explosive. The model was evaluated by hematoxylin and eosin staining and pathological score. The inflammation and oxidative stress of lung injury were also investigated. The pathological scores of rats’ lung injury at 2 h, 24 h, 3 days, and 7 days post-blast were 9.75±2.96, 13.00±1.85, 8.50±1.51, and 4.00±1.41, respectively, which were significantly increased compared with those in the control group (1.13±0.64; P&lt;0.05). The respiratory frequency and pause were increased significantly, while minute expiratory volume, inspiratory time, and inspiratory peak flow rate were decreased in a time-dependent manner at 2 and 24 h post-blast compared with those in the control group. In addition, the expressions of inflammatory factors such as interleukin (IL)-6, IL-8, FosB, and NF-κB were increased significantly at 2 h and peaked at 24 h, which gradually decreased after 3 days and returned to normal in 2 weeks. The levels of total antioxidant capacity, total superoxide dismutase, and glutathione peroxidase were significantly decreased 24 h after the shock wave blast. Conversely, the malondialdehyde level reached the peak at 24 h. These results indicated that inflammatory and oxidative stress induced by shock waves changed significantly in a time-dependent manner, which may be the important factors and novel therapeutic targets for the treatment of BLI.

scholarly journals Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

2014 ◽  
Vol 25 (6) ◽  
pp. 461-465
Author(s):  
João Paulo De Carli ◽  
Soluete Oliveira da Silva ◽  
Maria Salete Sandini Linden ◽  
Carmen Silvia Busin ◽  
Luiz Renato Paranhos ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Clinical Signs ◽  
Hcv Infection ◽  
Control Group ◽  
Proliferative Potential ◽  
Group 2 ◽  
Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

The Association between Oxidative Stress and Cardiac Functions in Infants Born to Preeclamptic Mothers

2018 ◽  
Vol 36 (11) ◽  
pp. 1205-1210
Author(s):  
Didem Arman ◽  
Secil Ercin ◽  
Sevilay Topcuoğlu ◽  
Ayşem Kaya ◽  
Taner Yavuz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Premature Infants ◽  
Stress Index ◽  
Control Group ◽  
Cardiac Functions ◽  
Total Antioxidant ◽  
Echocardiographic Parameters ◽  
Oxidant Status ◽  
The Relationship ◽  
And Oxidative Stress

Objective The present study aimed to assess the global oxidant and antioxidant status in infants born to preeclamptic mothers and their correlation with cardiac functions. Study Design We compared 40 infants born to preeclamptic mothers with 40 premature infants born to normotensive mothers. We assessed the relationship between echocardiographic measurements and total antioxidant capacity (TAC) and total oxidant status (TOS) values. Results In the study group, TAC, TOS, and oxidative stress index (OSI) levels were significantly higher in the cord blood (p = 0.03, 0.04, and 0.039, respectively) than in the control group. We did not observe any correlation between echocardiographic measurements and TAC, TOS, and OSI levels in infants born to preeclamptic mothers. Conclusion Compared with the control group, despite higher TAC levels in infants born to preeclamptic mothers, concurrent elevated OSI levels reveal that the oxidant–antioxidant balance is disturbed in favor of oxidants. Furthermore, the findings of this study suggest that echocardiographic parameters are unaffected by the oxidant status.

scholarly journals Salivary Cortisol Levels in Patients with Oral Lichen Planus—A Pilot Case-Control Study

2019 ◽  
Vol 7 (2) ◽  
pp. 59 ◽  
Author(s):  
Ivana Skrinjar ◽  
Valentina Vidranski ◽  
Bozana Loncar Brzak ◽  
Danica Vidovic Juras ◽  
Ana Andabak Rogulj ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Salivary Cortisol ◽  
Oral Lichen Planus ◽  
Case Control Study ◽  
Case Control ◽  
Healthy Controls ◽  
Unstimulated Saliva ◽  
Cortisol Levels ◽  
Oral Lichen ◽  
Control Study

It is known that cortisol level increases in stress situations. The aim of the study was to measure the levels of salivary cortisol in patients with oral lichen planus (OLP) and healthy controls. This was a case-control pilot study which included seven patients with reticular (non-symptomatic) OLP, eight patients with atrophic/erosive (symptomatic) OLP, and nine healthy controls. We hypothesized that patients with an atrophic/erosive type of OLP have higher levels of cortisol compared to patients with the reticular type of OLP and healthy controls. In each participant, unstimulated saliva was collected in order to determine cortisol levels by using commercially available ELISA kit. Our results have shown no differences between levels of salivary cortisol in OLP patients and healthy controls. We can conclude that further research with a larger number of OLP patients is needed to determine the correlation between OLP and stress.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals The treatment of the lysozyme-containing drugs of patients with oral lichen planus together with dental pathology

2016 ◽  
Author(s):  
O. V. Yeliseyeva
Keyword(s):  
Lipid Peroxidation ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Alveolar Bone ◽  
Chronic Inflammatory Disease ◽  
Pathogenic Agent ◽  
Dental Pathology ◽  
The Common ◽  
Oral Lichen

Prevalence and severity of generalized parodontitis depend especially on the course of diseases affecting theoral mucosa and those ones which are characterized by involvement of the oral mucosa. These diseases include mainly lichen planus. The common pathogenic agent of generalized parodontitis and lichen planus as well as of combination of them is inflammation, as generalized parodontitis is inflammation of parardontium tissue characterized by destruction of periodontium and dental ligamentous apparatus of the alveolar bone and lichen planus is a chronic inflammatory disease of skin and oral mucosa. The purpose is to study processes of lipid peroxidation in the saliva of patients with lichen planus of the skin along with chronic generalized parodontitis.

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