scholarly journals The effect of the edein complex on the activity of apical root meristems of Allium cepa L.

2015 ◽  
Vol 44 (4) ◽  
pp. 589-595
Author(s):  
J. A. Tarkowska ◽  
K. B. Domańska
Keyword(s):  
Dna Synthesis ◽  
Allium Cepa ◽  
Water Solution ◽  
Bacterial Cells ◽  
Root Tips ◽  
Irreversible Damage ◽  
Allium Cepa L ◽  
Good Accordance ◽  
Meristematic Activity ◽  
Inhibition Of Dna Synthesis

The effect of a mixture of edeins in water solution on the meristematic activity of <em>Allium cepa</em> L. root tips was studied during the incubation and postincubation periods. Reduction of the elongation growth of the root tips and a strong decrease in. the number of mitoses was observed what, as cytochemical and autoradiographical studies have shown, is due to the inhibition of DNA synthesis. DNA synthesis is completely inhibited after 24 hrs of incubation. Changes caused by 25 ppm concentration of the solution are reversible during postincubation. The concentration of 50 ppm produces irreversible damage. The main direction of cytochemical changes caused by the action of edeins on the meristematic cells is in good accordance with those recorded for bacterial cells (Kuryło-Borowska 1962, Borowski and Chmara 1968).

scholarly journals Mitotic activity, submicroscopic organization and respiration of meristematic cells of root tips of Allium cepa L. treated with dacarbazine (DTIC)

2014 ◽  
Vol 51 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Maria Podbielkowska ◽  
Anna Śluborska ◽  
Maria Wałęza
Keyword(s):  
Dna Synthesis ◽  
Mitotic Activity ◽  
Cell Respiration ◽  
Root Tips ◽  
Experimental Conditions ◽  
Respiratory Inhibitors ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Inhibition Of Dna Synthesis ◽  
Tissue Ultrastructure

Studies were carried out on the effect of dacarbazine (DTIC) on meristematic cells of root tips of <em>Allium</em> cepa L. Noticeable mitodepression was observed. It depended upon time of exposure and DTIC concentration. Mitodepression was caused by an inhibition of DNA synthesis and blocking of the G<sub>2</sub> phase. DTIC caused also disturbances of the karyokinesis and cytokinesis, similar as in case of colchicine effect. In the experimental conditions inhibition of cell respiration was noted, as also changes in the tissue ultrastructure. These changes were typical of the effect of respiratory inhibitors.

scholarly journals PENAMBAHAN LARUTAN BAWANG MERAH (Allium cepa L.) DAN AIR KELAPA (Cocos nucifera L.) SEBAGAI FITOHORMON ALAMI PADA PERTUMBUHAN TANAMAN TEBU (Sacchanum officinarum L.) SEBAGAI SUMBER BELAJAR BIOLOGI

2020 ◽  
Vol 11 (2) ◽  
pp. 160
Author(s):  
Dewi Kurniawati ◽  
HRA Mulyani ◽  
Rasuane Noor
Keyword(s):  
Allium Cepa ◽  
Water Solution ◽  
Cocos Nucifera ◽  
Coconut Water ◽  
The Third ◽  
Randomized Design ◽  
Allium Cepa L ◽  
Number Of Leaves ◽  
The One ◽  
Cocos Nucifera L

This study aims to 1) to see the effect of natural phytohormones in onions and coconut water on the growth of sugarcane, 2) To see the effect of giving natural phytohormones to sugarcane plants which produce the best growth, 3) To see the results of research on the growth of sugarcane can be used biology learning resources. This type of research is an experimental study using a completely randomized design (CRD). This study consisted of 4 treatments, namely, the first treatment was giving sand and red soil, the second treatment was giving manure and 15% concentration of onion solution phytohormones, the third treatment was giving manure and phytohormones with 25% concentration of coconut water solution, the fourth treatment was Pembrian. Manure and phytohormone combined with 15% concentration of onion solution + phytohormone with 25% concentration of coconut water solution. The parameters observed in this study were height in units (cm), number of leaves (counting manually), stem diameter in units (cm). To determine the effect of phytohormone solutions on shallots and coconut water, data analysis was carried out using the one-way ANOVA test. and further tests to find out the most optimum treatment for the growth of sugarcane using the BNJ test. Based on the research, there was a significant effect of giving natural phytohormones on the growth of sugarcane, with the best treatment, namely the third treatment of giving manure and phytohormones of coconut water solution. The results of this research can be used as a source of learning biology in the form of videos with material on the growth and development of class XII. Kata kunci: larutan bawang merah (allium cepa L), air  kelapa (cocos nucifera L), fitohormon, tanamantebu.

scholarly journals Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots

2014 ◽  
Vol 71 (3) ◽  
pp. 211-221 ◽  
Author(s):  
Agnieszka Majewska ◽  
Mirosława Furmanowa ◽  
Kazimierz Głowniak ◽  
Joanna Guzewska ◽  
Alicja Zobel ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Chromatin Condensation ◽  
Preprophase Band ◽  
Taxus Baccata ◽  
Cell Nuclei ◽  
Root Tips ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Interphase Cells ◽  
Average Size

We investigated the influence of extract from <em>Taxus baccata</em> var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of <em>Allium cepa</em> L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.

Cytotoxicity of the Fungicides Azoxystrobin and Difenoconazole in Root Tips of Allium cepa L.

2005 ◽  
Vol 1 (1) ◽  
pp. 65-80 ◽  
Author(s):  
S. Nithyameen ◽  
P.R. Jeyaramraja . ◽  
S. Manian .
Keyword(s):  
Allium Cepa ◽  
Root Tips ◽  
Allium Cepa L

scholarly journals Genotoxic Effects of Raxil on Root Tips and Anthers of Allium cepa L.

Caryologia ◽  
2009 ◽  
Vol 62 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Kaymak Fisun ◽  
Pinar Goc Rasgele
Keyword(s):  
Allium Cepa ◽  
Root Tips ◽  
Genotoxic Effects ◽  
Allium Cepa L

scholarly journals Effect of Ferrous Gluconate on Chromosomal Abnormality Index of Allium Cepa Root Tip

2021 ◽  
Vol 9 (4) ◽  
pp. 755-758
Author(s):  
Nergis Kaya
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Abnormality ◽  
Genotoxic Effect ◽  
Root Tip ◽  
Control Group ◽  
Root Tips ◽  
Ferrous Gluconate ◽  
Allium Cepa L ◽  
Abnormality Index

In completed research, ferrous gluconate -a food additive- used to preserve black color to prevent discoloration during storage in ripe black olives, and Allium cepa L. species. A. cepa L. roots were treated with different doses of ferrous gluconate. The effective concentration EC50 (0.068 g/l) was determined. A. cepa root tips were treated with EC50/2 (0.034 g/l), EC50 (0.068 g/l), 2XEC50 (0.136 g/l) dose for 24, 48, 72 hours, and afterward, the root tips were prepared for observation under the light microscope according to the method of preparing mitotic preparation. Chromosomal abnormality index (CAI) and genotoxic effect of ferrous gluconate in A. cepa root tip cells were determined. Repeated measurement ANOVA and TUKEY multiple comparison tests were used to investigate the effect of time and dose together on genotoxicity. C-mitosis, polyploidy, polar shifting in anaphase, polar shifting in telophase, equatorial plate shifting, laggard chromosome was observed by microscope. The highest CAI (70.16±4.85) was observed at 72h for 2XEC50 dose. Chromosomal aberration is also observed in control group. While the most common chromosomal aberration is determined as C-mitosis; The least observed chromosomal aberration is determined as polyploidy. Research results revealed that ferrous gluconate has a genotoxic effect on the root tip of A. cepa.

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