scholarly journals The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

2015 ◽  
Vol 42 (3) ◽  
pp. 111 ◽  
Author(s):  
Bahia Namavar Jahromi ◽  
Zahra Mosallanezhad ◽  
Najmeh Matloob ◽  
Maryam Davari ◽  
Mohamed Amin Ghobadifar
Keyword(s):  
Embryo Development ◽  
Granulosa Cells ◽  
Potential Role ◽  
Culture Study ◽  
Human Oocytes ◽  
Maturation Rate

scholarly journals Increased number of multi-oocyte follicles (MOFs) in juvenile p27Kip1 mutant mice: potential role of granulosa cells

2013 ◽  
Vol 28 (4) ◽  
pp. 1023-1030 ◽  
Author(s):  
J. Perez-Sanz ◽  
J. Arluzea ◽  
R. Matorras ◽  
N. Gonzalez-Santiago ◽  
J. Bilbao ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Potential Role ◽  
Mutant Mice

scholarly journals Potential role of hCG in apoptosis of human luteinized granulosa cells

2015 ◽  
Vol 61 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Rei HIRATA ◽  
Takuo HOJO ◽  
Masahiro SANO ◽  
Nobuyoshi HAYASHI ◽  
Kiyoshi OKUDA
Keyword(s):  
Granulosa Cells ◽  
Potential Role

Potential role of microRNAs in mammalian female fertility

2017 ◽  
Vol 29 (1) ◽  
pp. 8 ◽  
Author(s):  
Dawit Tesfaye ◽  
Dessie Salilew-Wondim ◽  
Samuel Gebremedhn ◽  
Md Mahmodul Hasan Sohel ◽  
Hari Om Pandey ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Potential Role ◽  
Mammalian Species ◽  
Female Fertility ◽  
Conditional Knockout ◽  
Female Reproduction ◽  
Extracellular Mirnas ◽  
Mammalian Female

Since the first evidence for the involvement of microRNAs (miRNAs) in various reproductive processes through conditional knockout of DICER, several studies have been conducted to investigate the expression pattern and role of miRNAs in ovarian follicular development, oocyte maturation, embryo development, embryo–maternal communication, pregnancy establishment and various reproductive diseases. Although advances in sequencing technology have fuelled miRNA studies in mammalian species, the presence of extracellular miRNAs in various biological fluids, including follicular fluid, blood plasma, urine and milk among others, has opened a new door in miRNA research for their use as diagnostic markers. This review presents data related to the identification and expression analysis of cellular miRNA in mammalian female fertility associated with ovarian folliculogenesis, oocyte maturation, preimplantation embryo development and embryo implantation. In addition, the relevance of miRNAs to female reproductive disorders, including polycystic ovary syndrome (PCOS), endometritis and abnormal pregnancies, is discussed for various mammalian species. Most importantly, the mechanism of release and the role of extracellular miRNAs in cell–cell communication and their potential role as non-invasive markers in female fertility are discussed in detail. Understanding this layer of regulation in female reproduction processes will pave the way to understanding the genetic regulation of female fertility in mammalian species.

Effect of potential role of p53 on embryo development arrest induced by H2O2 in mouse

2017 ◽  
Vol 53 (4) ◽  
pp. 344-353 ◽  
Author(s):  
De-Bao Hu ◽  
Zhong-Shu Li ◽  
Ihsan Ali ◽  
Li-Jie Xu ◽  
Nan-Zhu Fang
Keyword(s):  
Embryo Development ◽  
Potential Role

scholarly journals Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

2020 ◽  
pp. 1
Author(s):  
Tulay Irez ◽  
Sinem Ercan Dogan ◽  
Enver Ciraci ◽  
Saadet Busra Aksoyer ◽  
Muhammet Sait Toprak ◽  
...  
Keyword(s):  
Experimental Study ◽  
Luteinizing Hormone ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Germinal Vesicle ◽  
Cumulus Cells ◽  
Immature Oocytes ◽  
Maturation Rate

<p><strong>OBJECTIVE:</strong> In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. </p><p><strong>STUDY DESIGN:</strong> In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. </p><p><strong>RESULTS:</strong> In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p&gt; 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).</p><p><strong>CONCLUSION:</strong> In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.</p>

scholarly journals The Potential Role of As-sumo-1 in the Embryonic Diapause Process and Early Embryo Development of Artemia sinica

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85343 ◽  
Author(s):  
Bing Chu ◽  
Feng Yao ◽  
Cheng Cheng ◽  
Yang Wu ◽  
Yanli Mei ◽  
...  
Keyword(s):  
Embryo Development ◽  
Potential Role ◽  
Early Embryo ◽  
Embryonic Diapause ◽  
Artemia Sinica ◽  
Early Embryo Development

Potential role of intraspecific and interspecific cloning in the conservation of wild mammals

Zygote ◽  
2019 ◽  
Vol 27 (3) ◽  
pp. 111-117 ◽  
Author(s):  
Alana Azevedo Borges ◽  
Alexsandra Fernandes Pereira
Keyword(s):  
Somatic Cell ◽  
Embryo Development ◽  
Potential Role ◽  
Donor Cell ◽  
Cell Nuclei ◽  
Wild Mammals ◽  
Donor Cells ◽  
Cell Banks ◽  
Future Work

SummaryIntraspecific and interspecific cloning via somatic cell nuclear transfer (iSCNT) is a biotechnique with great possibilities for wild mammals because it allows the maintenance of biodiversity by recovering species, nuclear reprogramming for the production of pluripotency-induced cells, and studies related to embryonic development. Nevertheless, many areas in cloning, especially those associated with wild mammals, are still in question because of the difficulty in obtaining cytoplasmic donor cells (or cytoplasts). Conversely, donor cell nuclei (or karyoplasts) are widely obtained from the skin of living or post-mortem individuals and often maintained in somatic cell banks. Moreover, the creation of karyoplast–cytoplast complexes by fusion followed by activation and embryo development is one of the most difficult steps that requires further clarification to avoid genetic failures. Although difficult, cloning different species, such as wild carnivores and ungulates, can be successful via iSCNT with embryo development and the birth of offspring. Thus, novel research in the area that contributes to the conservation of biodiversity and knowledge of the physiology of species continues. The present review presents the failures and successes that occurred with the application of the technique in wild mammals, with the goal of helping future work on cloning via iSCNT.

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