Identification of Ensis siliqua Samples and Establishment of the Catch Area Using a Species-Specific Microsatellite Marker

2012 ◽  
Vol 95 (3) ◽  
pp. 820-823 ◽  
Author(s):  
Juan Fernández-Tajes ◽  
Aalberto Arias-Pérez ◽  
Miguel B Gaspar ◽  
Josefina Méndez
Keyword(s):  
Microsatellite Marker ◽  
Species Identification ◽  
Production Method ◽  
European Council ◽  
Base Pairs ◽  
Razor Clam ◽  
Ensis Siliqua ◽  
Fishery Products ◽  
Council Regulation ◽  
Species Specific

Abstract European Council Regulation 104/2000 states that fishery products must be labeled to indicate commercial designation of species, the production method, and the catch area. Therefore, traceability of seafood implies knowledge of the species offered to retail and their origin. Ensissiliqua is a bivalve intensively fished in Europe and sold in fresh and canned forms. Although several published methods clearly differentiate Ensis genus species, none of those assess the origin of the commercial samples. In the present study, a microsatellite marker (Esi-UDC3055F) was developed to establish the catch area of E. siliqua samples. Amplification yielded a fragment of 275 or 302 base pairs, depending on whether they were Iberian or Irish populations. The usefulness of this method was also assessed in commercial samples. The results of this study provide a reliable methodology for the identification of catch area in European E. siliqua commercial samples. The coupling of this methodology with existing techniques for razor clam species identification provides a powerful tool for traceability and labeling enforcement.

SEROLOGICAL AND MOLECULAR EVALUATION OF MIGRATORY TRICHINELLA SPIRALIS LARVAE IN BLOOD OF HUMANS IN KADUNA METROPOLIS, NIGERIA

2021 ◽  
Vol 4 (4) ◽  
pp. 281-289
Author(s):  
Paul Isaac Ojodale ◽  
Helen Ileigo Inabo ◽  
Elijah Ekah Ella ◽  
Oluyinka Oluseyi Okubanjo
Keyword(s):  
Trichinella Spiralis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Seroprevalence Rate ◽  
Base Pairs ◽  
Worldwide Distribution ◽  
Food Borne ◽  
Atp6 Gene ◽  
Amplicon Size ◽  
Polymerase Chain ◽  
Species Specific

Trichinellosis is an important food-borne zoonotic disease with public health implications and a worldwide distribution. In this study, Polymerase Chain Reaction (PCR) procedure using species specific ATP6 primers was used to detect the presence of migratory Trichinella spiralis larval mitochondrial ATP6 synthase F0 subunit (ATP6) gene, after detection of antibodies to Trichinella excretory-secretory (E/S) antigen using Enzyme-linked Immunosorbent Assay (ELISA), in blood of humans in Kaduna metropolis, Nigeria. The sera of 210 participants were tested for antibodies to Trichinella E/S antigen. Overall seroprevalence rate of 39% (82/210) was recorded using ELISA. Out of the 9 ELISA samples selected randomly, PCR detected migratory Trichinella spiralis larval ATP6 gene in 4 (44.4%) at the amplicon size of 250 base pairs using the whole blood of the participants.  The 9 samples comprised 7 seropositive and 2 seronegative. The bands at lanes 1, 2, 3 and 4 were positive for ATP6 while lanes 5,6,7,8 and 9 were negative for ATP6.  Lanes 4 and 5 were ELISA negative for anti-Trichinella antibodies. One in 5 of the 128 ELISA negative samples was positive for ATP6 representing a 25.6% prevalence rate by extrapolation.  PCR using ATP6 gene as a genetic marker is valuable for the detection of T. spiralis migratory larvae in blood samples of humans and consequently the early diagnosis of trichinellosis in humans.

scholarly journals Rapid Species Identification of Cooked Poisonous Mushrooms by Using Real-Time PCR

2008 ◽  
Vol 74 (10) ◽  
pp. 3306-3309 ◽  
Author(s):  
Kazuhiko Maeta ◽  
Tomoya Ochi ◽  
Keisuke Tokimoto ◽  
Norihiro Shimomura ◽  
Nitaro Maekawa ◽  
...  
Keyword(s):  
Real Time ◽  
Species Identification ◽  
Real Time Pcr ◽  
Specific Test ◽  
Specific Identification ◽  
Specific Fluorescence ◽  
Species Specific ◽  
Poisonous Mushrooms

ABSTRACT Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.

Rapid Fish Species Identification by Agarose Gel Isoelectric Focusing of Sarcoplasmic Proteins

1981 ◽  
Vol 64 (1) ◽  
pp. 38-43
Author(s):  
Ronald C Lundstrom
Keyword(s):  
Species Identification ◽  
Isoelectric Focusing ◽  
Fish Species ◽  
Protein Pattern ◽  
Agarose Gel ◽  
Tissue Fluid ◽  
Protein Patterns ◽  
Fish Species Identification ◽  
Species Specific ◽  
Gel Isoelectric Focusing

Abstract A rapid method is described for fish species identification by agarose gel isoelectric focusing (AGIEF). The AGIEF method can be completed in less than 2 h and gives reproducible species-specific sarcoplasmic protein patterns. Protein patterns are similar using either centrifuged tissue fluid or muscle tissue as the sample. One species, monkfish (Lophius americanus), has a polymorphic protein pattern. A predominant pattern was found in 66.7% of the individuals; 2 variant patterns were equally distributed among the remaining 33.3%. AGIEF offers a more rapid, less expensive alternative to the current AOAC official first action method for fish species identification based on polyacrylamide gel isoelectric focusing.

scholarly journals Human homologs of TU transposon sequences: polypurine/polypyrimidine sequence elements that can alter DNA conformation in vitro and in vivo.

1986 ◽  
Vol 6 (11) ◽  
pp. 3632-3642 ◽  
Author(s):  
B Hoffman-Liebermann ◽  
D Liebermann ◽  
A Troutt ◽  
L H Kedes ◽  
S N Cohen
Keyword(s):  
Tandem Repeats ◽  
Base Pairs ◽  
S1 Nuclease ◽  
Human Dna ◽  
Outer Domain ◽  
Sequence Elements ◽  
Dna Strands ◽  
Species Specific

We previously have shown that homologs of the outer domain segment of the inverted repeat termini (IVR-OD) of the sea urchin TU transposons are conserved among multiple eucaryotic species, including humans. We report here that two cloned human DNA IVR-OD homologs, Hut2 and Hut17, consist of a series of tandem repeats of the trimer AGG/TCC, forming segments (313 and 221 base pairs in length, respectively) of polypurine/polypyrimidine (pPu/pPy or "Puppy") asymmetry in the two DNA strands; these are punctuated at certain sites with variant trimers, which are different for the two clones. Sequences homologous to the Hut2 pPu/pPy tract exist at multiple sites in the DNA of a wide variety of eucaryotes. Hybridization of human DNA with a Hut2 probe or with a previously described chicken DNA pPu/pPy sequence indicates that pPu/pPy sequences can be grouped into families distinguishable by the extent of their homology with each probe at different hybridization stringencies. Moreover, particular pPu/pPy tracts show species-specific differences in their distribution. Both the Hut2 and Hut17 pPu/pPy tracts are cleaved by S1 nuclease when tested on supercoiled plasmids. Most if not all of the 313-base-pair Hut2 pPu/pPy tract is also sensitive to S1 in its native location in HeLa cell chromatin, indicating that the sequence contains conformational information that can be expressed in vivo. This view is supported by evidence that exogenously derived Hut2 pPu/pPy tracts introduced into mouse L cells and integrated in chromatin can assume an S1-sensitive conformation.

scholarly journals Evaluation of Peroxidase in Herbal Medicines Based on an Electrochemical Sensor

2021 ◽  
Vol 9 ◽  
Author(s):  
Yinzi Yue ◽  
Lianlin Su ◽  
Min Hao ◽  
Wenting Li ◽  
Li Zeng ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Electrochemical Sensor ◽  
Species Identification ◽  
Modified Electrode ◽  
Colorimetric Method ◽  
Herbal Medicines ◽  
Detection Technique ◽  
Plant Tissues ◽  
Electrocatalytic Reduction ◽  
Species Specific

Peroxidases are species-specific. Differences in peroxidase can objectively reflect the genetics among species. The use of peroxidase to assist in species identification is relatively simple and effective. In this work, we proposed a graphene-modified electrode. This electrode can amplify the signal of electrocatalytic reduction of hydrogen peroxide. Since peroxidase can catalyze the reduction of hydrogen peroxide, this signal can be used as an indicator to demonstrate the content of peroxidase in different plant tissues. Twelve herbal medicines were selected for our study. The results show that this electrochemical-based detection technique was comparable to colorimetric method in terms of accuracy.

Sign in / Sign up

Export Citation Format

Share Document