scholarly journals A comparative study of cellular morphology and nuclear DNA content on uterine squamous cell carcinomas by flow cytometry and fluorescence cytophotometry.

1984 ◽  
Vol 23 (4) ◽  
pp. 503-508
Author(s):  
Michio SUGITA ◽  
Tetsuya MUROYA ◽  
Tadashi SUGISHITA ◽  
Yoshio TENJIN ◽  
Yoshio NAGASHIMA
Keyword(s):  
Flow Cytometry ◽  
Comparative Study ◽  
Squamous Cell ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Squamous Cell Carcinomas ◽  
Cellular Morphology

Nuclear DNA content using computerized image cytometry of squamous cell carcinomas of the head and neck

1997 ◽  
Vol 111 (1) ◽  
pp. 43-47 ◽  
Author(s):  
S. K. Sarker ◽  
K. Ghufoor ◽  
K. S. Patel ◽  
N. S. Tolley ◽  
D. V. Coleman
Keyword(s):  
Flow Cytometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Nuclear Dna ◽  
Clinical Stage ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Squamous Cell Carcinomas ◽  
Histological Differentiation ◽  
Ploidy Status

AbstractPloidy status using flow cytometry of head and neck cancers may be of prognostic value. We describe the use of image cytometry in ploidy measurement of squamous cell carcinomas of the head and neck (SCCHN). This technique allows only tumour cells to be measured, thereby rejecting debris, artefact and benign cells. Tissue sections were cut from tumours and then Feulgen stained. A total of 60 patients were included in this study, 23 females and 37 males. The data reveals a relationship between ploidy status and the histological differentiation. However, the ploidy status and histological differentiation do not appear to correlate to the clinical stage of the disease. This method of measuring ploidy may be more accurate than flow cytometry and may have a prognostic role in head and neck cancer patients. A study comparing both methods may demonstrate this and we aim to evaluate this in the future.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals Brief communication. First evaluation of nuclear DNA content in Setaria genus by flow cytometry

1998 ◽  
Vol 89 (6) ◽  
pp. 556-559 ◽  
Author(s):  
M Le Thierry d'Ennequin ◽  
O Panaud ◽  
S Brown ◽  
S Siljak-Yakovlev ◽  
A Sarr
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

Identification of chromosomal deficiency by flow cytometry and cytogenetics in mutant tomato (Solanum lycopersicum, Solanaceae) plants

2009 ◽  
Vol 57 (5) ◽  
pp. 444 ◽  
Author(s):  
Isane Vera Karsburg ◽  
Carlos Roberto Carvalho ◽  
Wellington Ronildo Clarindo
Keyword(s):  
Flow Cytometry ◽  
Solanum Lycopersicum ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome 1 ◽  
Wild Type ◽  
Extrinsic Factors ◽  
Solanum Lycopersicum L ◽  
Morphogenic Process

Structural chromosomal aberrations can occur spontaneously in plant karyotypes as a result of both intrinsic and extrinsic factors. These aberrations may affect sporophyte fitness because fundamental genes involved with distinct morphogenic process may be lost. Inadequate development of flowers and anomalous fruits without seeds has been observed in plants of Solanum lycopersicum L. (Solanaceae) ‘BHG 160’ of the tomato germplasm bank (Universidade Federal de Viçosa, Brazil). The nuclear DNA content, quantified by flow cytometry, showed that mutant ‘BHG 160’ possesses 0.09 pg (4.59%) less nuclear DNA content than does the wild-type ‘BGH 160’. Improved cytogenetical preparations evidenced that this difference was due to a spontaneous terminal deficiency in the short arm of the mutant ‘BGH 160’ Chromosome 1. These results suggest that the genes encoded in the short arm of Chromosome 1 may be involved in the development of flowers and fruits in the tomato.

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