scholarly journals Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma

2021 ◽  
Vol 71 (5) ◽  
pp. 365-377
Author(s):  
Aleksandra Jeremić ◽  
Filip Milosavljević ◽  
Sandra Vladimirov ◽  
Bojan Batinić ◽  
Bojan Marković ◽  
...  
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Dynamic Range ◽  
Psychiatric Patients ◽  
Relative Difference ◽  
Sample Volume ◽  
Therapeutic Drug ◽  
Simultaneous Quantification ◽  
Electrospray Source

Simultaneous quantification of multiple psychiatric drugs is important for the therapeutic drug monitoring of psychiatric patients. In addition, it would be highly advantageous if the method could be simple, straightforward, and not time-consuming. A 200 µl plasma sample was deproteinized, drugs were separated by a ZORBAX Eclipse XDB-Phenyl column with the mobile phase composed of acetonitrile and 0.1 % formic acid in water (60:40, v/v), and recorded in the MRM mode by using a positive electrospray source with tandem mass spectrometry detection. The dynamic range was 2-256 ng/ml for all the analyzed drugs, except escitalopram (8-256 ng/ml). Quality control samples were prepared in quintuplicates in three relevant concentrations for each drug. Coefficients of determination (R2 ) were higher than 0.99, while the relative difference between nominal and measured concentrations (RE) and CV were lower than 15% for all targets. High performance liquid chromatography coupled with the mass detector (HPLC-MS/MS) method for simultaneous determination of sertraline, escitalopram, risperidone and paliperidone in human plasma was validated with respect to selectivity, linearity, accuracy, precision, matrix effect and stability. This method has significant advantages in terms of low sample volume (200 µl), short preparation time (3 hours) and short runtime per sample (4 minutes).

Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays

Bioanalysis ◽  
2019 ◽  
Vol 11 (21) ◽  
pp. 1917-1925 ◽  
Author(s):  
Yuhuan Ji ◽  
Yijiang Liu ◽  
Wanhong Xia ◽  
Alexander Behling ◽  
Min Meng ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Human Plasma ◽  
Peptide Nucleic Acid ◽  
Dynamic Range ◽  
Sample Volume ◽  
Probe Design ◽  
Assay Sensitivity ◽  
Accuracy And Precision ◽  
Fluorescence Assays

Aim: The importance of the length and/or structure of fluorescently labeled PNA (peptide nucleic acid) probes for quantitative determination of oligodeoxynucleotides (ODNs) is demonstrated in human plasma using hybridization-based LC-fluorescence assays. The length of the PNA probes impacts the peak shape and chromatographic separation of the resulting PNA/ODN hybridization complexes and affects assay sensitivity, dynamic range and carryover. Methods: For quantitative determination of an 18-mer phosphodiester ODN (DNL1818) in human plasma, an assay utilizing an Atto dye-labeled 12-mer PNA probe provided a linear quantitation range of 0.1–50 ng/ml with excellent accuracy and precision (within -5.3–7.73%). Conclusion: This method provides a convenient method for sensitive and specific quantification of ODNs in biological matrix with limited sample volume and no special extraction.

A UPLC-MS/MS Assay for Simultaneous Determination of Two Antipsychotics and Two Antidepressants in Human Plasma and Its Application in Clinic

2020 ◽  
Vol 21 (1) ◽  
pp. 60-69
Author(s):  
Houli Li ◽  
Xiaoliang Cheng ◽  
Di Zhang ◽  
Maoyi Wang ◽  
Weihua Dong ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
High Performance ◽  
Medication Compliance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Individual Therapy ◽  
Therapeutic Drug ◽  
Ionization Source

Background: Antidepressants and antipsychotics are widely prescribed drugs for the treatment of mental diseases. Therapeutic drug monitoring (TDM) is recommended for patients taking these drugs to ensure pharmaceutical efficacy, medication compliance and prevent toxicity. Objective: An ultra-high performance liquid chromatography/tandem-mass spectrometry (UPLC-MS/ MS) method was developed for simultaneous determination of two Antidepressants-Fluoxetine (FLU) and Escitalopram (ESC), and two antipsychotics-risperidone (RIS) and aripiprazole (ARI), in human plasma. Methods: The sample was processed by simple protein precipitation and the targeted analytes were separated on a C18 column by gradient elution with a mobile phase containing 0.1% formic acid (v/v) and acetonitrile. All the analytes were qualitative and quantitative measured by electrospray ionization source with Multiple Reaction Monitoring (MRM) in positive ion mode. A total of 56 plasma samples were obtained from out- or in-patients who were taking the cited four drugs for further analysis. Results: The calibration curves for FLU, ESC, RIS and ARI were linear in the range of 45-1800, 4-320, 2-200 and 50-1800 ng/mL, respectively. The entire analytical time for the analytes was 7.0 min for each run and the extraction efficiency was more than 90%. The sample was stable within various storage conditions. The trough concentrations in patients were measured with the validated method. Conclusions: The developed method was successfully used for simultaneous determination of FLU, ESC, RIS and ARI in the plasma of the patients, which provides effective technical support for routine TDM of these four drugs and is of great clinic value for individual therapy.

scholarly journals High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Determination of Arbidol in Human Plasma

2011 ◽  
Vol 94 (4) ◽  
pp. 1100-1105 ◽  
Author(s):  
Xin Xiong ◽  
Suodi Zhai
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Internal Standard ◽  
Sample Volume ◽  
Tandem Mass ◽  
Spectrometry Method ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Butyl Methyl Ether

Abstract An HPLC/MS/MS method for the determination of arbidol in human plasma was developed. Arbidol and internal standard (loratadine) were extracted from alkaline plasma with tert-butyl methyl ether and analyzed on a Zorbax SB C18 column (30 × 2.1 mm id, 3.5 µm particle size). The detection was by monitoring arbidol at m/z 479.1 → 434.1 and the internal standard at m/z 383.2 → 337.2. The method was validated according to U.S. Food and Drug Administration guidelines. The calibration curve was linear over the range of 0.5–500 ng/mL using a 100 µL sample volume. The intraday and interday precisions were less than 6.5%, and acceptable values were obtained for accuracy, recovery, and sensitivity. The developed method was selective, simple, sensitive, and easily applicable.

Determination of fluoxetine and norfluoxetine in human plasma by high-performance liquid chromatography with ultraviolet detection in psychiatric patients

2003 ◽  
Vol 783 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Adrián LLerena ◽  
Pedro Dorado ◽  
Roland Berecz ◽  
Antonio González ◽  
Marı́a Jesús Norberto ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Psychiatric Patients ◽  
Ultraviolet Detection

scholarly journals Ultra High Performance Liquid Chromatography Method for the Determination of Two Recently FDA Approved TKIs in Human Plasma Using Diode Array Detection

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Marwa Fouad ◽  
Maxime Helvenstein ◽  
Bertrand Blankert
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Tyrosine Kinase ◽  
Human Plasma ◽  
Tyrosine Kinase Inhibitors ◽  
High Performance ◽  
Kinase Inhibitors ◽  
Therapeutic Window ◽  
Therapeutic Drug

Generally, tyrosine kinase inhibitors have narrow therapeutic window and large interpatient variability compared to intrapatient variability. In order to support its therapeutic drug monitoring, two fast and accurate methods were developed for the determination of recently FDA approved anticancer tyrosine kinase inhibitors, afatinib and ibrutinib, in human plasma using ultra high performance liquid chromatography coupled to PDA detection. Diclofenac sodium was used as internal standard. The chromatographic separation was achieved on an Acquity UPLC BEH C18 analytical column using a mobile phase combining ammonium formate buffer and acetonitrile at a constant flow rate of 0.4 mL/min using gradient elution mode. AµSPE (solid phase extraction) procedure, using Oasis MCXµElution plates, was processed and it gave satisfying and reproducible results in terms of extraction yields. Additionally, the methods were successfully validated using the accuracy profiles approach (β= 95% and acceptance limits = ±15%) over the ranges 5–250 ng/mL for afatinib and from 5 to 400 ng/mL for ibrutinib in human plasma.

scholarly journals A simple HPLC-UV Method for Therapeutic Drug Monitoring of Linezolid in human Plasma in low-resourced settings

2021 ◽  
Vol 7 (4) ◽  
pp. e21008-e21008
Author(s):  
Vijayakumar A ◽  
Sudha V ◽  
Alffenaar JW ◽  
Jeyakumar SM ◽  
Hemanth Kumar AK
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Routine Care ◽  
Therapeutic Drug ◽  
Chromatography Method ◽  
Resistant Tuberculosis ◽  
Multi Drug Resistant Tuberculosis ◽  
Relative Standard ◽  
Liquid Chromatography Method

OBJECTIVE: A high-performance liquid chromatography method for the estimation of Linezolid in human plasma was developed and validated. METHODS: Samples (100µµL) were deproteinized with acetonitrile and analyzed using LiChrospher 100, RP18e column with PDA detection at 254 nm. The flow rate of the isocratic mobile phase comprising of 0.1% formic acid in 1000 ml of water and acetonitrile in the ratio of 60:40 (v/v) was set at 1.0 ml/min. RESULTS: The calibration curve ranged from 0.50 to 20.0 µg/ml and was linear. The recovery ranged from 96% to 101%. The accuracy ranged from 98 to 101% and intra- and inter-day relative standard deviation was <4.58%. The method reliably eliminated interfering materials from plasma and R2 was 0.9973. The method described was applied to the determination of plasma LZD concentration in multi-drug-resistant tuberculosis patients who are treated with a dose of 600 mg LZD once daily. CONCLUSIONS: The developed method is suitable for determination of plasma LZD in routine care and considered feasible in less-resourced settings

scholarly journals HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ASSAY FOR DETERMINATION OF MOXIFLOXACIN IN HUMAN PLASMA

2019 ◽  
Vol 57 (3) ◽  
pp. 336
Author(s):  
Luong Thi My Hanh ◽  
Nguyen Thi Minh Diep ◽  
Pham Thi Ngoc Mai ◽  
Nguyen Xuan Truong
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Sample Pretreatment ◽  
Reversed Phase ◽  
Hplc Method ◽  
Single Step ◽  
Therapeutic Drug ◽  
Uv Detector ◽  
Coefficient Corrélation

A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.

scholarly journals Determination of Metformin in Human Plasma and Urine by High-Performance Liquid Chromatography Using Small Sample Volume and Conventional Octadecyl Silane Column

2010 ◽  
Vol 13 (4) ◽  
pp. 486 ◽  
Author(s):  
Dion Brocks ◽  
Raniah Q. Gabr ◽  
Raj S. Padwal
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Sodium Dodecyl ◽  
Extraction Procedure ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Sample Volume ◽  
Small Sample ◽  
Human Plasma And Urine

Purpose: To develop a selective and sensitive high-performance liquid chromatographic method for the determination of metformin in human plasma and urine, using a conventional reverse phase column and low specimen volume. Methods: Extraction of metformin and ranitidine (as internal standard) from plasma and urine samples (100 µL) was performed with a 1-butanol-hexane (50:50, v/v) mixture under alkaline conditions followed by back-extraction into diluted acetic acid. Chromatography was carried out using a C18 column (250 mm×4.6 mm, 5 μm). A mobile phase consisting of acetonitrile and KH2PO4 (34:66, v/v) and sodium dodecyl sulphate (3 mM) was pumped at an isocratic flow rate of 0.7 mL/min. Results: The calibration curves were linear (>0.995) in the concentration ranges of 10–5000 and 2–2000 μg/mL for metformin in plasma and urine respectively. The mean absolute recoveries for 100 and 1000 ng/mL metformin in plasma using the present extraction procedure were 93.7 and 88.5%, respectively. The intra- and inter-day coefficients of variation in plasma and urine were

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