Progeny analysis of transgenic rice variety transformed withGlyoxalase Igene

2014 ◽  
Vol 7 (4) ◽  
pp. 769
Author(s):  
Chhaya Sharma ◽  
Maninder Kaur ◽  
Ajinder Kaur ◽  
SS Gosal
Keyword(s):  
Transgenic Rice ◽  
Rice Variety ◽  
Progeny Analysis

scholarly journals Perkembangan Pradewasa dan Kemampuan Hidup Predator Verania lineata Thurnberg (Coleoptera: Coccinellidae) pada Tanaman Padi Varietas Rojolele Transgenik

2016 ◽  
Vol 34 (3) ◽  
pp. 187
Author(s):  
N. Usyati ◽  
Damayanti Buchori ◽  
Syafrida Manuwoto ◽  
Purnama Hidayat ◽  
Inez H. Slamet Loedin
Keyword(s):  
Transgenic Rice ◽  
Natural Enemies ◽  
Transgenic Line ◽  
Developmental Stages ◽  
Negative Impact ◽  
Rice Variety ◽  
Research Centre ◽  
Insect Predator ◽  
The Impact ◽  
Larvae Development

<p>The use of transgenic varieties on the agricultural production system may provide some agronomical benefits. However, uses of transgenic variety have raised some debates about their potential negative impact on the environment, such as on the decreasing of natural enemies. To study the impact of transgenic variety to the natural enemies, study was conducted on larvae development, and the survival of predatoral insect (V. lineata) on the transgenic Rojolele rice variety. Test was conducted at the laboratory of Molecular Biology, Research Centre for Biotechnology of Indonesian Institute of Science, from January to October 2009. Completely randomized design with 8 treatments and 30 replications were employed. The treatments were transgenic Rojolele rice as follow: 4.2.3-28-15-2-7 and 4.2.4-21-8-16-4 lines contained fusion of two cry genes (cryIB-cryIAa). The 3R9-8-28-26-2 and 3R7-8-15-2-7 lines contained mpi::cryIB gene, the T9-6.11-420 line contained cryIAb gene obtained by particle bombardment, DTcry (azygous) is a segregate and does not contain cry gene (null), DTcry-13 line contained cryIAb gene by Agrobacterium, and non transgenic rice (Rojolele variety). Results showed that there were differences of larvae development and survival of insect predator V. lineata fed on the among transgenic rice lines. On transgenic line T9-6.11-420 and on DTcry-13 line the life time, developmental stages, the number eclosion of adult female, adult insect weight, and survival of the preimaginal and the adult of insect predator were consistently low. On the transgenic line 4.2.3-28-15-2-7; 3R9-8-28-26-2; and 3R7-8-15-2-7 each had no consistent effect on the larvae development and the survival of insect predator. DTcry (azygous) line had no effect on the larvae development and the survival of insect predator. Whereas transgenic line 4.2.4-21-8-16-4 had an effect on the adult weight of insect predator.</p>

scholarly journals Deteksi Gen HptII dan Keragaan Agronomis pada Populasi BC1F1 Tanaman Padi Transgenik

2016 ◽  
Vol 9 (3) ◽  
pp. 117
Author(s):  
Budi Santosa ◽  
Kurniawan R. Trijatmiko ◽  
Tri J. Santoso
Keyword(s):  
Drought Stress ◽  
Transgenic Rice ◽  
Stress Condition ◽  
Rice Variety ◽  
Agronomic Performance ◽  
Lower Side ◽  
Cultivated Rice ◽  
Rice Varieties ◽  
Drought Stress Condition ◽  
Donor Parent

<p>Rice varieties tolerant to drought stress are needed to<br />stabilize rice production under drought stress condition. We<br />developed transgenic rice cv. Nipponbare carrying hptII<br />gene that might also contain OsDREB1A gene. OsDREB1A<br />gene responsible to drought tolerance trait need to be<br />transferred into cultivated rice in order to obtain new local<br />rice variety tolerant to drought stress. The aims of this<br />research were to detect the presence of hptII gene in the F1<br />and BC1F1 transgenic rice and to observe the agronomic<br />performace of those populations and their plant physiology.<br />F1 population was developed by crossing transgenic<br />Nipponbare, as donor parent, with Batutegi, Code, Ciherang,<br />and Konawe genotypes, as recipient parents. BC1F1<br />population was developed by backcrossing F1 transgenic<br />line with recipient parents, respectively. The presence of<br />hptII gene was analyzed by PCR using a pair of primers for<br />hptII. The observation of agronomic performance was<br />carried out in the green house, meanwhile the observation<br />of stomata was done using microscope. The result of PCR<br />analysis showed that BC1F1 Batutegi trans, BC1F1 Code trans,<br />BC1F1 Konawe trans1, BC1F1 Konawe trans3, dan BC1F1<br />Konawe trans4 were detected carrying the hptII gene.<br />Agronomic data showed that BC1F1 transgenic rice lines<br />yielded panicles, filled grains, and total grains higher than<br />those of recipent parents. Comparing to the recipient<br />parents, BC1F1 Konawe trans1 and BC1F1 Konawe trans3 had<br />less stomata on the lower side of the leaf, but had more<br />stomata on the upper side of the leaf.</p>

Transgenic rice variety ‘IR72’ with Xa21 is resistant to bacterial blight

1998 ◽  
Vol 97 (1-2) ◽  
pp. 31-36 ◽  
Author(s):  
J. Tu ◽  
I. Ona ◽  
Q. Zhang ◽  
T. W. Mew ◽  
G. S. Khush ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Bacterial Blight ◽  
Rice Variety

Lowland Rice Yield as Affected by Straw Incorporation and Inorganic Fertilizer Over Cropping Seasons in Fluvisol

2014 ◽  
Vol 3 (1) ◽  
pp. 129-141
Author(s):  
Brahima Koné ◽  
Zadi Florent ◽  
Gala bi Trazié Jeremie ◽  
Akassimadou Edja Fulgence ◽  
Konan Kouamé Firmin ◽  
...  
Keyword(s):  
Grain Yield ◽  
Rice Variety ◽  
Mineral Fertilizer ◽  
Lowland Rice ◽  
Inorganic Fertilizer ◽  
Cropping Season ◽  
Significant Difference ◽  
Straw Incorporation ◽  
Cropping Seasons ◽  
Fertilizer Effect

Grain yield stabilization of lowland rice over cropping seasons was explored using different compositions of inorganic fertilizers (NPK, NPKCa, NPKMg, NPKZn, NPKCaMg, NPKCaZn and NPKCaMgZn) and straw incorporation (3, 6, 9, 12 and 15 tha-1 ). No fertilizer and no straw amended plot was the control in a split-plot design with three replications laid in a Fluvisol of Guinea savanna in Centre Cote d’Ivoire. Three weeks old nursery rice variety NERICA L19 was transplanted. No significant difference of grain yield was observed between the different treatments excluding the highest yields recorded for treatments NPKMg (5.09 tha-1 ), NPKZn (5.15 tha-1 ) and NPKCaéMg (5.31 tha-1 ) compared with 12 (3.95 tha1 ) and 15 tha-1 (4.14 tha-1 ) as straw rates respectively. Grain yield declining trend was more pronounced for mineral fertilizer treatments showing twice greater depressive effect of cropping cycle compared with the straw especially, for treatments characterized by highest grain yield in the first cropping season and similar grain yields were recorded for both sources of nutrient in the third cropping cycle. Of slowness of nutrients releasing by straw, highest grain yield was expected for this soil amender within a longer period of cultivation whereas, unbalance soil micronutrients should be relevant to studious declining yield under inorganic fertilizer effect. Nevertheless, the straw rate of 12 tha-1 supplying 0.58% of NPK as mineral fertilizer equivalent can be recommended for sustaining lowland rice production in the studied agro-ecosystems unless for three cropping seasons.

Marker assisted selection for confirming F1 plants for tidal tolerant rice variety development

2020 ◽  
Vol 4 (3) ◽  
pp. 668-678
Author(s):  
Mithun Saha ◽  
Md. Niuz Morshed Khan ◽  
Sujan Kumar Kundu ◽  
Md. Monirul Islam ◽  
Sabina Yasmin ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Rice Variety ◽  
Selection For

Glycaemic responses to glucose and rice in people of Chinese and European ethnicity

2020 ◽  
Author(s):  
M Kataoka ◽  
BJ Venn ◽  
SM Williams ◽  
Lisa Te Morenga ◽  
IM Heemels ◽  
...  
Keyword(s):  
Ethnic Differences ◽  
Rice Variety ◽  
Glycaemic Index ◽  
Activity Levels ◽  
Glycaemic Response ◽  
Rice Varieties ◽  
Adjusted Risk ◽  
Postprandial Glycaemia ◽  
Adjusted Risk Ratio ◽  
Glucose Curve

Aims: Diabetes rates are especially high in China. Risk of Type 2 diabetes increases with high intakes of white rice, a staple food of Chinese people. Ethnic differences in postprandial glycaemia have been reported. We compared glycaemic responses to glucose and five rice varieties in people of European and Chinese ethnicity and examined possible determinants of ethnic differences in postprandial glycaemia. Methods: Self-identified Chinese (n = 32) and European (n = 31) healthy volunteers attended on eight occasions for studies following ingestion of glucose and jasmine, basmati, brown, Doongara® and parboiled rice. In addition to measuring glycaemic response, we investigated physical activity levels, extent of chewing of rice and salivary α-amylase activity to determine whether these measures explained any differences in postprandial glycaemia. Results: Glycaemic response, measured by incremental area under the glucose curve, was over 60% greater for the five rice varieties (P < 0.001) and 39% greater for glucose (P < 0.004) amongst Chinese compared with Europeans. The calculated glycaemic index was approximately 20% greater for rice varieties other than basmati (P = 0.01 to 0.05). Ethnicity [adjusted risk ratio 1.4 (1.2-1.8) P < 0.001] and rice variety were the only important determinants of incremental area under the glucose curve. Conclusions: Glycaemic responses following ingestion of glucose and several rice varieties are appreciably greater in Chinese compared with Europeans, suggesting the need to review recommendations regarding dietary carbohydrate amongst rice-eating populations at high risk of diabetes. © 2012 Diabetes UK.

Implementing the systems approach in rice pest management : India context

2019 ◽  
Vol 56 (Special Issue) ◽  
pp. 136-142 ◽  
Author(s):  
RK Tanwar ◽  
S Singh ◽  
SP Singh ◽  
Vikas Kumar Kanwar ◽  
Rakesh Kumar ◽  
...  
Keyword(s):  
Systems Approach ◽  
Insect Pests ◽  
Rice Variety ◽  
National Level ◽  
Uttar Pradesh ◽  
Fine Tuning ◽  
Basmati Rice ◽  
Rice Grain ◽  
Integrated Crop Management ◽  
Chemical Pesticides

A basic IPM module for basmati rice as a part of integrated crop management accounting the pest prevalence and the information available from literature has been developed. As the module is location specific and dynamic, therefore needs regular updating and fine tuning depending on the location and pest prevalence. The technology has been successfully validated and implemented during 1998 to 2019 in Pusa Basmati 1, Taraori Basmati, Dehraduni Basmati, Pusa Basmati 1121 in Haryana, Uttar Pradesh and Uttarakhand with little modification and fine tuning. Implementation of the technology resulted in increase in rice grain yield i.e., 21.6% in Pusa Basmati 1 at Shikohpur, in Uttar Pradesh 21.5% in Taraori Basmati at Chhajpur in Haryana, 19.5% in Type 3 in Uttarakhand, 14.5 to 22.7% in Pusa Basmati 1121 in Haryana and 38.2% in Pusa Basmati 1121 in Uttar Pradesh over farmer's practices (FP). In all the basmati rice trials, higher yield as well as Benefit - Cost (B-C) ratio was obtained in IPM as compared to FP. Implementation of IPM led to significant reduction in uses of chemical pesticides. In case of IPM, only 1.46 application of chemical pesticides (103.2 g a.i./ha) were undertaken in Pusa Basmati 1121 against 2.8 application in FP (1214.4 g a.i./ha) at Bambawad, Uttar Pradesh. ICAR-NRRI, Cuttack developed and validated IPM module at Sigmapur (Cuttack, Odisha) non-Basmati rice variety Pooja for four kharif seasons i.e 2010-2013 using standard agronomic practices. The trial resulted in an enhancement of 47.1% yield and 26.47 % B-C ratio over FP. Additional income of Rs. 9857/- per ha was also obtained in IPM over FP. On-Farm trial on validation of IPM module in non-Basmati rice (Pooja variety) under semi deep water conditions conducted in 10 ha during rabi 2017 at Nagapur and Basudeipur villages in Khurda district of Odisha resulted in reduction in the incidence of insect pests and diseases with higher population of natural enemies as compared to FP. Rice yield was recorded 5600 kg ha-1 in IPM as against 4900 kg ha-1 in FP. Recently, ICT based e-pest monitoring and advisory has become an important component for area wise implementation of IPM at state and national level. The programme has been successfully implemented in Odisha during 2010 and 2011 under RKVY. Implementation of the programme had resulted successful management of swarming caterpillar in 13 rice growing districts of Odisha during kharif 2010 and 2011.

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