Difference in biofilm development capability of vancomycin and ciprofloxacin resistant Staphylococcus aureus clinical isolates

The Staphylococcus aureus FnBPA and FnBPB proteins promote acid-induced biofilm accumulation. Meticillin-resistant S. aureus (MRSA) isolates from device-related infections with both fnbA and fnbB produced significantly more biofilm than isolates with either gene alone. Under mildly acidic growth conditions, FnBP-mediated biofilm and fnbA and fnbB transcript levels were substantially higher during growth at 37 °C than at 30 °C. Thus, in addition to a lowered pH, carriage of both fnbA and fnbB and growth at 37 °C promote MRSA biofilm development, further supporting a role for the FnBPA and FnBPB surface proteins in the pathogenesis of MRSA device-related infections.

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Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds

Journal of Biomaterials Applications ◽

10.1177/0885328218770530 ◽

2018 ◽

Vol 33 (1) ◽

pp. 82-93

Author(s):

Phat L Tran ◽

Jianqiang Li ◽

Lisa Lungaro ◽

Srikanthan Ramesh ◽

Ilia N Ivanov ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Wound Infection ◽

Zinc Sulfide ◽

Laser Scanning Microscopy ◽

Clinical Isolates ◽

Aureus Strain ◽

Inhibition Assay ◽

Zone Of Inhibition ◽

Methicillin Resistant ◽

Biofilm Development

Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This study examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus ( S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Colony forming cell assay and in vivo live imaging results strongly suggested the inhibition of S. aureus growth.

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Antibacterial activity of unifloral honeys against clinical isolates of methicillin-resistant Staphylococcus aureus

Planta Medica ◽

10.1055/s-0029-1234974 ◽

2009 ◽

Vol 75 (09) ◽

Author(s):

A Hannan ◽

MB Hussain ◽

M Absar

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Clinical Isolates ◽

Methicillin Resistant

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Assessment of the Multifactorial Effect on Antimicrobial Sensitivity in Positive Staphylococcus Aureus Clinical Isolates from Assir Region, Saudi Arabia

Journal of Medicine ◽

10.3329/jom.v13i2.12750 ◽

2012 ◽

Vol 13 (2) ◽

pp. 152-159 ◽

Cited By ~ 2

Author(s):

Nazar M Abdalla ◽

Waleed O Haimour ◽

Amani A Osman ◽

Hassan Abdul Aziz

Keyword(s):

Staphylococcus Aureus ◽

Saudi Arabia ◽

Culture Media ◽

Meca Gene ◽

Laboratory Data ◽

Age Groups ◽

Clinical Isolates ◽

Diabetic Patients ◽

Staph Aureus ◽

Antimicrobial Sensitivity

General objectives: This study aimed at assessment of factors affecting antimicrobial sensitivity in Staphylococcus aureus clinical isolates from Assir region, Saudi Arabia. Materials and Methods: In this study, eighty one patients presented with Staph. aureus infections either nosocomial or community acquired infections were involved by collecting nasal swabs from them at Aseer Central Hospital General Lab. These patients were from all age groups and from males and females during the period of Jan 2011- Jun 2011. These samples were undergone variable laboratory procedures mainly; bactech, culture media, antibiotics sensitivity test using diffusion disc test (MIC) and molecular (PCR) for detection of mec A gene. Clinical and laboratory data were recorded in special formats and analyzed by statistical computer program (SPSS). Results: Showed that; Descriptive and analytical statistical analysis were performed and final results were plotted in tables. In Staph aureus MecA gene positive cases (50) showed: Oxacillin/ Mithicillin, Ciprofloxacin and Fusidin resistant in diabetic patients were 13, 26.0%, 9, 18% and 7, 14% respectively and in non diabetic patients were 37, 74.0%, 22, 44% and 20, 40% respectively. While no sensitivity in diabetic and non diabetic patients using Oxacillin/ Mithicillin. In Staph aureus MecA gene negative cases (31) showed: Oxacillin/ Mithicillin, sensitivity in diabetic patients (5, 16.1%) and in non diabetic were (26, 83.9%). While no resistant in diabetic and non diabetic patients. In Ciprofloxacin and Fusidin resistant in diabetic patients were 1, 3.2% and 1, 3.2% respectively and in non diabetic patients were 12, 38.7% and 7, 22.6%respectively. Erythromycin in Staph aureus ( MecA gene) positive cases (50) showed: resistant in age (0-15) years were (5, 10%), (16-50) years were (16, 32%) and ( 50 years) were (12, 24%). Erythromycin in Staph aureus (MecA gene) negative cases (31) showed: resistant in age (0-15) years were (6, 19.3%), (16-50) years were (5, 16.1%) and ( 50 years) were (3, 9.7%). Conclusion: Drugs resistance is a major progressive multifactorial problem facing the treatment of Staph aureus infections. DOI: http://dx.doi.org/10.3329/jom.v13i2.12750 J Medicine 2012; 13 : 152-159

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