In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines

Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with ethyl acetate and in its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by nuclear magnetic resonance (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatography (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10−7 M) and MCF-7 cells (IC50 5.10−6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the ethyl acetate extract can exert a significant dose dependent in vitro cytotoxicity.

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In vitro antioxidant and cytotoxic activities of polyherbal extracts from Vetiveria zizanioides, Trichosanthes cucumerina, and Mollugo cerviana on HeLa and MCF-7 cell lines

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2020.04.005 ◽

2020 ◽

Vol 27 (6) ◽

pp. 1475-1481

Author(s):

Vidya Devanathadesikan Seshadri ◽

P. Vijayaraghavan ◽

Y.-O. Kim ◽

H.-J. Kim ◽

Abdullah Ahmed Al-Ghamdi ◽

...

Keyword(s):

Cell Lines ◽

Cytotoxic Activities ◽

Vetiveria Zizanioides ◽

Mcf 7

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In vitro Evaluation of Antimicrobial and Cytotoxic Activities of Rosmarinus officinalis L. (Lamiaceae) Essential Oil Cultivated from South-West Tunisia

Journal of Applied Pharmaceutical Science ◽

10.7324/japs.2012.21107 ◽

2012 ◽

Author(s):

Ines Ben Chobba

Keyword(s):

Essential Oil ◽

Rosmarinus Officinalis ◽

Cytotoxic Activities ◽

In Vitro Evaluation ◽

South West ◽

Rosmarinus Officinalis L

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Modeling of antiproliferative effects of Salvia officinalis L. essential oil optimized using Box–Behnken design

10.21203/rs.3.rs-44076/v1 ◽

2020 ◽

Author(s):

Imen Kallel ◽

Ahmed Bayoudh ◽

Bochra Gargouri ◽

Lamia Khannous ◽

Asma Elaguel ◽

...

Keyword(s):

Essential Oil ◽

Cell Viability ◽

Cell Lines ◽

Human Cancer ◽

Salvia Officinalis ◽

Dependent Manner ◽

Box Behnken Design ◽

Cytotoxicity Activity ◽

Mcf 7

Abstract Background Salvia officinalis L. essential oil (SoEO) was mostly traditionally used to medicate various diseases as cancer. Then, the present work aims were: (1) to model the cytotoxicity effects of Salvia officinalis L. essential oil (SoEO) related to the human cancer cell lines kind (MCF-7 and HeLa) ; (2) to optimize the hydro-distillation extraction conditions of SoEO; and, (3) to determine the in vitro scavenging capacity of the free radicals DPPH•, NO•, ABTS+, and the ability to reduce Fe3+. Methods The cytotoxicity and anti-proliferative abilities were evaluated by measuring cell viability and then modeled. Two human cell lines: MCF-7 and HeLa were used. The optimization of SoEO extraction by hydro-distillation was carried out with Response Surface Methodology (RSM) using the Box–Behnken design Results The cytotoxicity activity against both tumor cell lines MCF-7 and HeLa was considerably important with IC50 = 3.125 and 8.920 µg/mL, respectively. All treated cell lines showed a significant reducing in cell viability in response to the increasing oil concentration. The relative behaviors of both cell lines under SoEO treatment were modeled. The obtained optimal extraction yield was Y = 1.85 g/100 g d.b. The main identified fractions were camphene (23.7%), α-thujone (19.62%), 1,8-cineole (10.6%), viridiflorol (5.9%), borneol (5.72%); β-thujone (5.4%); caryophyllene (3,83%). Also, SoEO was mostly able to scavenge DPPH• free radical, ABTS+ radical and hydrogen peroxide in an amount dependent manner (IC50 = 0.97, 0.279 and 0.05 mg/mL, respectively). Conclusion The present work provides a preliminary platform for further investigation of the possible mechanism of S. officinalis essential oils and their individual compounds in cytotoxic and antitumor activity.

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In Vitro Evaluation of Cytotoxic Activities of Marketed Herbal Products in Ghana

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x18790723 ◽

2018 ◽

Vol 23 ◽

pp. 2515690X1879072 ◽

Cited By ~ 2

Author(s):

Sylvester Languon ◽

Isaac Tuffour ◽

Emmanuel Ekow Quayson ◽

Regina Appiah-Opong ◽

Osbourne Quaye

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Jurkat Cells ◽

Total Phenolic ◽

Cytotoxic Activities ◽

Hep G2 ◽

Herbal Products ◽

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There are numerous herbal products on the Ghanaian market that are purported to cure various ailments, including cancer. However, scientific investigations on efficacy and toxicity of most of these products are not done. The aim of the study was to assess the anticancer potentials of herbal products on the Ghanaian market. Antiproliferative effects of Kantinka BA (K-BA), Kantinka Herbaltics (K-HER), Centre of Awareness (COA), a stomach (STO) and multicancer (MUT) product were evaluated in vitro using liver (Hep G2), breast (MCF-7), prostate (PC-3 and LNCaP), and blood (Jurkat) cancer cell lines. Cytotoxicity of the medicinal products was assessed using tetrazolium-based colorimetric assay, and total phenolic content and antioxidant activity of the products were determined using Folin-Ciocalteau and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Phytochemical screening resulted in the detection of terpenoids and flavonoids in most of the products, and alkaloids were detected in only MUT. Tannins were absent from all the products. The highest and lowest concentrations of phenolics were recorded for MUT and K-BA, respectively. The highest and lowest antioxidant activities were measured for MUT and K-HER, respectively. Only 2 products (STO and MUT) were cytotoxic to Hep G2 cells; with MUT being the only product that was cytotoxic to MCF-7 cells. All but K-BA were cytotoxic to PC-3 cells, while all products except K-HER were cytotoxic to LNCaP and Jurkat cells. The study thus confirms that the herbal products have selective cytotoxic activities against the tested cancer cell lines. However, comprehensive toxicity studies must be conducted to establish their safety.

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Two New Cytotoxic Steroidal Alkaloids from Sarcococca Hookeriana

Molecules ◽

10.3390/molecules24010011 ◽

2018 ◽

Vol 24 (1) ◽

pp. 11 ◽

Cited By ~ 1

Author(s):

Shaojie Huo ◽

Jichun Wu ◽

Xicheng He ◽

Lutai Pan ◽

Jiang Du

Keyword(s):

Cell Lines ◽

Human Cancer ◽

Cytotoxic Activities ◽

Steroidal Alkaloids ◽

X Ray ◽

Human Cancer Cell Lines ◽

X Ray Crystallography ◽

Ic50 Values ◽

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Two new steroidal alkaloids, named hookerianine A (1) and hookerianine B (2) were isolated from the stems and roots of Sarcococca hookeriana Baill., along with two known compounds, sarcorucinine G (3) and epipachysamine D (4). On the basis of spectroscopic methods and by comparison with literature data, their structures were determined. As well as X-ray crystallography was performed to confirm compound 4. To identify novel antitumor inhibitors, all compounds were performed a CCK-8 assay against five human cancer cell lines SW480, SMMC-7721, PC3, MCF-7 and K562 in vitro. Compound 2 exhibited moderate cytotoxic activities to all cell lines with IC50 values in the range of 5.97–19.44 μM. Compound 3 was the most effective one against SW480 and K562 cell lines with IC50 values of 5.77 and 6.29 μM, respectively.

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In Vitro Cytotoxic Activities of Platinum(II) Complex with 1-Methyl-2-(3'- hydroxypropyl)benzimidazole and 2-(3'-Hydroxypropyl)benzimidazolium Hexa- and Tetrachloroplatinate Salts

Letters in Drug Design & Discovery ◽

10.2174/1570180814666171012163325 ◽

2018 ◽

Vol 15 (1) ◽

pp. 65-69 ◽

Cited By ~ 2

Author(s):

Gokcen Eren ◽

Sukran Yilmaz ◽

Fatma Gumus

Keyword(s):

Cell Lines ◽

Antitumor Agents ◽

Platinum Complexes ◽

Inhibitory Effect ◽

Platinum Drugs ◽

Cytotoxic Activities ◽

Reference Compound ◽

Growth Inhibitory ◽

Mcf 7

Background: Platinum complexes as antitumor agents have received considerable attention due to the clinically approved and have been marketed worldwide such as cisplatin, carboplatin, oxaliplatin or marketed locally such as nedaplatin, lobaplatin and heptaplatin for treatment of various tumor diseases. However, those drugs still had their own defects and they exhibited side effects including nephrotoxicity, ototoxicity, neurotoxicity and bone marrow suppression. Therefore there is still continuing interest in the development of new platinum drugs avoiding their inherent resistance and toxicity. Methods: Two hexa- and tetrachloroplatinate salts and one platinum(II) complex were synthesized and evaluated for their in vitro cytotoxicities against human HeLa, MCF-7, and MDA-MB-231 cell lines by the MTT assay. Results: The growth inhibitory effect values of compounds 1-3, and cisplatin against the cell lines were expressed as inhibition %. Conclusion: Compound 3 showed appreciable activity for all examined cell lines compared to that of reference compound cisplatin.

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In vitro evaluation of radiolabeled (125I) methanol extracts of yarrow in cell lines of MCF-7, PC-3, A-549 and Caco-2

Journal of Radioanalytical and Nuclear Chemistry ◽

10.1007/s10967-012-1895-7 ◽

2012 ◽

Vol 295 (1) ◽

pp. 593-599 ◽

Cited By ~ 5

Author(s):

Ayfer Yurt Kilcar ◽

Betul Cekic ◽

Fazilet Zumrut Biber Muftuler ◽

Perihan Unak ◽

Emin Ilker Medine

Keyword(s):

Cell Lines ◽

In Vitro Evaluation ◽

Methanol Extracts ◽

Mcf 7

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In-vitro Antioxidant, Antidiabetic, Antibacterial and Cytotoxic Activities of Essential Oil extracted from Flowers of Illicium verum L.

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00431 ◽

2021 ◽

pp. 2452-2458

Author(s):

Monica Joicy. C ◽

Sivaraj. C ◽

Arumugam. P

Keyword(s):

Cell Death ◽

Essential Oil ◽

Reducing Power ◽

Assay Method ◽

Total Phenolic ◽

Cytotoxic Activities ◽

Star Anise ◽

Illicium Verum ◽

Mcf 7

Illicium verum is a small native evergreen tree of northeast Vietnam, India and southwest China. It is the flower of a medium-sized tree and is also known as star anise belongs to the family of Schisandraceae that grows in North America, Atlantic region, and tropical and subtropical zones of Asia. This study investigated the in vitro cytotoxic effects of essential oil obtained from the flowers of Illicium verum is also known as star anise. Steam distillation method was used to extract oil from the flowers of Illicium verum. Antioxidant activities such as DPPH (2,2-diphenyl-1-picrylhydrazyl), and ferric reducing power assays were assessed followed by total phenolic contents was estimated by folin-ciocalteau reagent method. Gas chromatography–mass spectrometry (GC-MS) analysis was used to find out volatile and semi-volatile compounds of essential oil extracted from Illicium verum. Anti-proliferative effect was studied by MTT assay method using MCF 7 and HepG 2 cancer cell lines. The maximum HepG 2 cell death was 25.73 ±0.24 % at 100µg/mL concentration and the IC50 was 194.32±0.24% at 100µg/mL concentration. The maximum MCF 7 cell death was 32.64±0.32% at 100µg/mL concentration and the IC50 was 153.18 ± 0.32% at 100µg/mL concentration.

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