scholarly journals Author response: Structural insights into human acid-sensing ion channel 1a inhibition by snake toxin mambalgin1

2020 ◽  
Author(s):  
Demeng Sun ◽  
Sanling Liu ◽  
Siyu Li ◽  
Mengge Zhang ◽  
Fan Yang ◽  
...  
Keyword(s):  
Ion Channel ◽  
Author Response ◽  
Human Acid ◽  
Snake Toxin ◽  
Structural Insights

scholarly journals Structural insights into human acid-sensing ion channel 1a inhibition by snake toxin mambalgin1

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Demeng Sun ◽  
Sanling Liu ◽  
Siyu Li ◽  
Mengge Zhang ◽  
Fan Yang ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Transmembrane Domain ◽  
Physiological Data ◽  
Closed State ◽  
Acid Sensing Ion Channels ◽  
Human Acid ◽  
Neuronal Processes ◽  
Peptide Toxin ◽  
Snake Toxin ◽  
Structural Insights

Acid-sensing ion channels (ASICs) are proton-gated cation channels that are involved in diverse neuronal processes including pain sensing. The peptide toxin Mambalgin1 (Mamba1) from black mamba snake venom can reversibly inhibit the conductance of ASICs, causing an analgesic effect. However, the detailed mechanism by which Mamba1 inhibits ASIC1s, especially how Mamba1 binding to the extracellular domain affects the conformational changes of the transmembrane domain of ASICs remains elusive. Here, we present single-particle cryo-EM structures of human ASIC1a (hASIC1a) and the hASIC1a-Mamba1 complex at resolutions of 3.56 and 3.90 Å, respectively. The structures revealed the inhibited conformation of hASIC1a upon Mamba1 binding. The combination of the structural and physiological data indicates that Mamba1 preferentially binds hASIC1a in a closed state and reduces the proton sensitivity of the channel, representing a closed-state trapping mechanism.

scholarly journals Psalmotoxin-1 Docking to Human Acid-sensing Ion Channel-1

2009 ◽  
Vol 284 (26) ◽  
pp. 17625-17633 ◽  
Author(s):  
Yawar J. Qadri ◽  
Bakhrom K. Berdiev ◽  
Yuhua Song ◽  
Howard L. Lippton ◽  
Catherine M. Fuller ◽  
...  
Keyword(s):  
Ion Channel ◽  
Human Acid

Characterisation of a human acid-sensing ion channel (hASIC1a) endogenously expressed in HEK293 cells

2001 ◽  
Vol 442 (5) ◽  
pp. 668-674 ◽  
Author(s):  
Gunthorpe M. ◽  
Smith G. ◽  
Davis J. ◽  
Randall A.
Keyword(s):  
Ion Channel ◽  
Hek293 Cells ◽  
Human Acid

scholarly journals Author response: Atypical calcium regulation of the PKD2-L1 polycystin ion channel

2016 ◽  
Author(s):  
Paul G DeCaen ◽  
Xiaowen Liu ◽  
Sunday Abiria ◽  
David E Clapham
Keyword(s):  
Ion Channel ◽  
Calcium Regulation ◽  
Author Response

scholarly journals Author response: Mechanistic signs of double-barreled structure in a fluoride ion channel

2016 ◽  
Author(s):  
Nicholas B Last ◽  
Ludmila Kolmakova-Partensky ◽  
Tania Shane ◽  
Christopher Miller
Keyword(s):  
Ion Channel ◽  
Author Response ◽  
Fluoride Ion

scholarly journals Author response: Unconventional secretory processing diversifies neuronal ion channel properties

2016 ◽  
Author(s):  
Cyril Hanus ◽  
Helene Geptin ◽  
Georgi Tushev ◽  
Sakshi Garg ◽  
Beatriz Alvarez-Castelao ◽  
...  
Keyword(s):  
Ion Channel ◽  
Author Response ◽  
Ion Channel Properties ◽  
Channel Properties
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