The Impact of Sharing Primer, the Quantity of the Internal Control Gene and the Primer Dimer on Reaction System in Duplex PCR

2019 ◽  
Vol 65 (12/2019) ◽  
Author(s):  
Wencan Jiang ◽  
Shang He ◽  
Jianan Wang ◽  
Chen Chen ◽  
Yingjiao Sha ◽  
...  
Keyword(s):  
Internal Control ◽  
Reaction System ◽  
Duplex Pcr ◽  
Control Gene ◽  
Internal Control Gene ◽  
The Impact

scholarly journals β-glucuronidase use as a single internal control gene may confound analysis in FMR1 mRNA toxicity studies

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192151 ◽  
Author(s):  
Claudine M. Kraan ◽  
Kim M. Cornish ◽  
Quang M. Bui ◽  
Xin Li ◽  
Howard R. Slater ◽  
...  
Keyword(s):  
Internal Control ◽  
Control Gene ◽  
Internal Control Gene ◽  
Toxicity Studies ◽  
Fmr1 Mrna

β-Glucuronidase is a suitable internal control gene for mRNA quantitation in pathophysiological and non-pathological livers

2013 ◽  
Vol 95 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Hiromi Yamaguchi ◽  
Sawako Matsumoto ◽  
Mariko Ishibashi ◽  
Kiyoshi Hasegawa ◽  
Masahiko Sugitani ◽  
...  
Keyword(s):  
Internal Control ◽  
Control Gene ◽  
Internal Control Gene ◽  
Mrna Quantitation

Selection of the Internal Control Gene for Real-Time Quantitative RT-PCR Assays in Temperature Treated Leptospira

2008 ◽  
Vol 56 (6) ◽  
pp. 539-546 ◽  
Author(s):  
Erika Margarita Carrillo-Casas ◽  
Rigoberto Hernández-Castro ◽  
Francisco Suárez-Güemes ◽  
Alejandro de la Peña-Moctezuma
Keyword(s):  
Real Time ◽  
Internal Control ◽  
Control Gene ◽  
Internal Control Gene ◽  
Rt Pcr ◽  
Pcr Assays ◽  
Selection Of

scholarly journals Determination of the reference genes for qRT-PCR normalization and expression levels of MAT genes under various conditions in Ulocladium

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10379
Author(s):  
Li-Guo Ma ◽  
Yun Geng
Keyword(s):  
Environmental Factors ◽  
Internal Control ◽  
Dna Sequences ◽  
Reference Genes ◽  
Culture Media ◽  
Control Gene ◽  
Internal Control Gene ◽  
Type Locus ◽  
Expression Levels ◽  
Qrt Pcr

The genus Ulocladium is thought to be strictly asexual. One of the possible reasons for the lack of sexuality in Ulocladium species is the absence of the stimulus of environmental factors. Sexual reproduction in ascomycetes is controlled by a specific region in the genome referred to as mating-type locus (MAT) that consists of two dissimilar DNA sequences in the mating partners, termed MAT1-1 and MAT1-2 idiomorphs. To identify the response of MAT loci to environmental conditions, the mRNA transcription level of MAT1-1-1 and MAT1-2-1 genes was tested using qRT-PCR under different temperatures (−20 °C, −10 °C, 0 °C, 10 °C, 20 °C, 30 °C and 40 °C), culture medias (CM, OA, HAY, PCA, PDA and V8), photoperiods (24 h light, 24 h dark, 12 h light/12 h dark, 10 h light/14 h dark and 8 h light/16 h dark), and CO2 concentrations (0.03%, 0.5%, 1%, 5%, 10%, 15% and 20%). For obtaining reliable results from qRT-PCR, the most stable internal control gene and optimal number of reference genes for normalization were determined under different treatments. The results showed that there is no universal internal control gene that is expressed at a constant level under different experimental treatments. In comparison to various incubation conditions, the relative expression levels of both MAT genes were significantly increased when fungal mycelia were grown on HAY culture media at 0–10 °C with a light/dark cycle, indicating that temperature, culture media, and light might be the key environmental factors for regulating the sexuality in Ulocladium. Moreover, MAT1-1-1 and MAT1-2-1 genes showed similar expression patterns under different treatments, suggesting that the two MAT genes might play an equally important role in the sexual evolutionary process.

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