scholarly journals ASSESSMENT OF ANTHELMINTIC ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF PSIDIUM GUAJAVA LEAVES: AN IN-VITRO DESIGN

2018 ◽  
Vol 7 (3) ◽  
pp. 82-87
Author(s):  
Swetha Naram Reddy ◽  
Susheel Rao Yeedulapally ◽  
Geetha Vani Palusam ◽  
Nikhil Pathi ◽  
Mani Swaroop Goud Gunala ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Psidium Guajava ◽  
Phytochemical Analysis

scholarly journals IN VITRO EVALUATION OF THE ANTHELMINTIC ACTIVITY OF RHIZOME EXTRACTS OF CURCUMA LONGA (LINN.)

2018 ◽  
Vol 11 (12) ◽  
pp. 425
Author(s):  
Jyoti Pandey ◽  
Suman Mishra ◽  
Kamal Jaiswal
Keyword(s):  
Curcuma Longa ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Alternative Source ◽  
Significant Efficacy ◽  
Phosphate Buffered Saline

Objective: The current study was carried out to evaluate the anthelmintic activity of the rhizome extract of Curcuma longa as an alternative source of effective remedies for nematodiasis.Methods: The anthelmintic activity of the C. longa was assessed in vitro against Haemonchus spp., a gastrointestinal (abomasum) parasite of goats. Different concentrations of the extracts (1 mg/mL, 2.5 mg/mL, 5 mg/mL, and 10 mg/mL) in phosphate-buffered saline (PBS) were tested, and the results expressed in terms of time of paralysis (minute) and time of death (minute) of the worms. Albendazole (1 mg/mL) was used as a reference (positive control) and PBS as a control group (negative control).Results: The qualitative phytochemical analysis of the methanolic extract (ME) of the plant disclosed the presence of alkaloids, glycosides, terpenoids, flavonoids, tannins, saponins, phenol, anthraquinone, and carbohydrates; whereas, the aqueous extract (AE) showed the presence of alkaloids, carbohydrate, flavonoids, and saponins. Both ME and AE of the C. longa (rhizome) expressed significant efficacy (p≤0.05) in causing paralysis as well as the death of the worms within 12 h of exposure at all tested concentrations, as compared to the negative control. The rhizome extracts of C. longa showed dose-dependent efficacy in causing paralysis of the worm motility and the final progression to death. The results showed that the ME at 10 mg/mL was significantly more potent (p≤0.05) over the AE.Conclusions: This study concluded that the rhizome extract of C. longa exhibited potent anthelmintic efficacy against the nematode parasite, Haemonchus spp.

scholarly journals Phytochemical analysis and in vitro anthelmintic activity of Imperata cylindrica underground parts

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Pawi Bawitlung Lalthanpuii ◽  
Kholhring Lalchhandama
Keyword(s):  
Plant Extract ◽  
Structural Changes ◽  
Anthelmintic Activity ◽  
The Body ◽  
Imperata Cylindrica ◽  
Gas Chromatography Mass Spectrometry ◽  
Intestinal Helminth ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus

Abstract Background Imperata cylindrica is considered as an agricultural weed, but it is a valuable plant in the traditional medicines of Southeast Asia. In the Mizo traditional medicine of India and Myanmar, the rhizomes and roots are used as a remedy for bacterial, fungal and intestinal helminth infections. Methods An extract of the whole underground parts was prepared in Soxhlet apparatus using chloroform as a solvent. After concentrating in a vacuum rotary evaporator, the extract was analysed using gas chromatography-mass spectrometry. Anthelmintic activity was tested in vitro against the tapeworm Raillietina tetragona and the roundworm Ascaridia galli. Scanning electron microscopy was used to examine the structural changes on the helminths after treatment with the plant extract. Results Twenty-two compounds were identified from the plant extract out of which fatty acids were the predominant compounds. Palmitic acid was the most abundant. Bioactive phytosterols such as campesterol and stigmasterol were also detected. The plant extract was significantly effective on both the helminths and showed dose-dependent anthelmintic activity as that of albendazole. The tapeworm treated with the plant extract showed deformities on the suckers, clumping of the spines, tegumental folds and erosion of microtriches. Extensive damage was also seen on the roundworm including cuticular shrinkage, collapse of the lips, and formation of warty surface throughout the body. Conclusion I. cylindrica extract effectively killed and caused detrimental effects on parasitic tapeworm and roundworm. The study therefore validates the traditional usage among the Mizo people, and guarantees further investigation on the exact compound(s) and mechanism of action.

In-vitro Anthelmintic Activity of Aqueous Extract of Leaves of Cleome Rutidosperma DC. (Capparidaceae) Against Haemonchus Contortus

2018 ◽  
Vol 4 (1) ◽  
pp. 415-418
Author(s):  
Md. Shahed-Al-Mahmud ◽  
Hasib Al Hasan
Keyword(s):  
Aqueous Extract ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Alternative Source ◽  
Standard Drug ◽  
Cleome Rutidosperma

Background: Cleome rutidosperma is annual herb found in different places in Bangladesh. Its leaves used as the treatment for Helminthiasis associated with Haemonchus contortus. This parasite is one of the most pathogenic nematodes and responsible for anemia, edema, and death of cattle’s, mainly during summer in warm, humid climates as like Bangladesh. The aim of the study to investigate the in-vitro anthelmintic activity of aqueous extract C. rutidosperma (AECR) leaves against H. contortus. Methods: We performed phytochemical analysis and in-vitro anthelmintic assay to determine the possible phytochemicals, caused by anthelmintic activity against H. contortus. Results: In-vitro anthelmintic assay, the AECR at the doses of 25; 50 and 100mg/mL significantly (p< 0.01) and (p< 0.001) paralyzed and caused the death of H. contortus in a dose-dependent manner. Reference standard drug Albendazole (15 mg/mL) exerts almost same effect as AECR. No paralyzed and death observed at the control group when treated with 0.9% normal saline. Conclusion: This data confirmed that the aqueous extract C. rutidosperma has the in-vitro anthelmintic activity of against H. contortus. C. rutidosperma may offer an alternative source for the control of gastrointestinal nematodes of cattle’s.

scholarly journals Uji Kandungan Senyawa Metabolit Sekunder pada Ekstrak Daun Jambu Biji (Psidium guajava L var. Pomifera) dari Kota Langsa, Aceh

2021 ◽  
Vol 3 (1) ◽  
pp. 12-18
Author(s):  
Reni Aisyah Simbolon ◽  
Halimatussakdiah Halimatussakdiah ◽  
Ulil Amna
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactobacillus Acidophilus ◽  
Oreochromis Niloticus ◽  
Psidium Guajava ◽  
Phytochemical Analysis ◽  
Annona Muricata ◽  
Steroid Saponin ◽  
Rhodomyrtus Tomentosa

Tumbuhan jambu biji (Psidium guajava L var. pomifera) adalah salah satu tanaman obat-obatan yang sering dimanfaatkan oleh masyarakat khususnya di Indonesia. Tumbuhan yang termasuk ke dalam famili Myrtaceae tersebut memiliki khasiat sebagai antidiare, antioksidan, antiinflamasi, dan antimikroba. Penelitian ini bertujuan untuk mengetahui kandungan senyawa metabolit sekunder yang terdapat dalam daun jambu biji merah (Psidium guajava  L. Var. Pomifera). Berdasarkan pengujian fitokimia yang telah dilakukan, menunjukkan bahwa daun kering positif mengandung senyawa steroid, saponin, fenol, dan tanin. Sedangkan pada daun segar positif mengandung senyawa alkaloid, steroid, saponin, fenol, dan tanin. Referensi : [1]       S. S. H. Aponno V. J., Yamlean Y. V. P., “Uji Efektivitas Sediaan Gel Ekstrak Etanol Daun Jambu Biji (Psidium guajava Linn) terhadap Penyembuhan Luka yang Terinfeksi Bakteri Staphylococcus Aureus pada Kelinci (Orytolagus cuniculus),” PHARMACON, vol. 3, no. 3, pp. 279–286, 2014, doi: 10.35799/pha.3.2014.5400. [2]       R. Rachmaniar, H. Kartamihardja, and Merry, “Pemanfaatan Buah Jambu Biji Merah (Psidium guajava Linn.) Sebagai Antioksidan Dalam Bentuk Granul Effervescent,” JSTFI Indones. J. Pharm. Sci. Technol., vol. 1, no. 5, pp. 1–20, 2016. [3]       Z. S. Desiyana S. L., Husni A. M., “Uji Efektivitas Sedian Gel Fraksi Etil Asetat Daun Jambu Biji (Psidium Guajava Linn) terhadap Penyenmbuhan Luka Terbuka pada Mencit (Mus musculus).,” J. Nat., vol. 16, no. 2, pp. 23–32, 2016. [4]       Rabbiyah F., “Pengaruh Pemberian Ekstrak Daun Jambu Biji (Psidium guajava Linn.) terhadap Pengikatan Trombosit pada Pasien Demam Berdarah Dengue,” J. Major., vol. 4, no. 7, pp. 91–96, 2015. [5]       T. Handayani, Witjaksono, and K. U. Nugraheni, “Induksi Tetraploid Pada Tanaman Jambu Biji Merah (Psidium guajava L.) secara In Vitro,” J. Biol. Indones., vol. 13, no. 2, pp. 271–278, 2017, doi: 10.47349/jbi/13022017/271. [6]       Y. Tampubolon R. T., “Pengaruh Formulasi Terhadap Sifat Fisik, Kimia, dan Organoleptik Effervescent Jambu Biji Merah (Psidium guajava var. Pomifera).,” J. Pangan dan Agroindustri, vol. 5, no. 3, pp. 27–37, 2017. [7]      S. A. Ariyani A. M. D., Santoso I. S., “Analisa Profitalitas Usaha Tani Jambu Biji Getas Merah di Kabupaten Kendal,” Acromedia, vol. 35, no. 2, pp. 10–18, 2017. [8]       I. S. W. Atmaja, Ismail Saleh, R. Eviyati, and D. Budirokhman, “Kajian Aplikasi Pupuk Kandang dan Pupuk Npk Terhadap Kualitas dan Mutu Jambu Biji Merah (Psidium guajava L.) Kultivar Getas pada Musim Kemarau,” J. Agrovigor, vol. 9, no. 2, pp. 111–117, 2016. [9]       C. Dhyan, S. H. Sumarlan, and B. Susilo, “Pengaruh Pelapisan Lilin Lebah dan Suhu Penyimpanan Terhadap Kualitas Buah Jambu Biji (Psidium Guajava L.),” J. Bioproses Komod. Trop., vol. 2, no. 1, pp. 79–90, 2014. [10]     W. N. Gunawan R., Susanto H. W., “Pengaruh Lama Pemanasan dan Konsentrasi Maizena terhadap Karakteristik Fisik, Kimia dan Organoleptik Lempok Jambu Biji Merah (Psidium Guajava L.),” J. Pangan dan Agroindustri, vol. 6, no. 1, pp. 1–11, 2018. [11]     D. N. Maria and E. Zubaidah, “Pembuatan Velva Jambu Biji Merah Probiotik (Lactobacillus Acidophilus) Kajian Persentase Penambahan Sukrosa dan CMC,” J. Pangan dan Agroindustri, vol. 2, no. 4, pp. 18–28, 2014. [12]     W. R. Andriani, “Efektivitas Mengkonsumsi Jus Apel Dibandingkan dengan Mengkonsumsi Jus Jambu Biji terhadap Penurunan Tingkat Halitosis,” AcTion Aceh Nutr. J., vol. 3, no. 2, pp. 164–171, 2018. [13]     F. Nadifah, S. Fatimah, and L. Susanti, “Pengaruh Infusa Daun Jambu Biji (Psidium guajava Linn.) Terhadap Pertumbuhan Bakteri Escherichia coli Secara In Vitro,” J. Heal., vol. 2, no. 2, pp. 65–68, 2015, doi: 10.30590/vol2-no2-p65-68. [14]     I. B. Wicaksono and M. Ulfah, “Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Sirsak (Annona muricata L.) dan Daun Jambu Biji (Psidium guajava L.) dengan Metode DPPH (2,2-difenil-1-pikrihidrazil),” Inov. Tek. Kim., vol. 2, no. 1, pp. 44–48, 2017. [15]     I. Hartati, S. Nurfaizin, Suwardiyono, and L. Kurniasari, “Ekstraksi Gelombang Mikro Terpenoid Daun Surian (Toona sureni Merr),” Inov. Tek. Kim., vol. 1, no. 2, pp. 98–103, 2016. [16]     A. U. Mulyanto S., Sumardianto, “Pengaruh Penambahan Ekstrak Daun Jambu Biji Merah (Psidium guajava) terhadap Daya Simpan Ikan Nila Merah (Oreochromis niloticus) pada Suhu Dingin,” J. Pengolah. dan Bioteknol. Has. Perikan., vol. 6, no. 4, pp. 1–7, 2018. [17]     H. Setiawan, L. B. Utami, and M. Zulfikar, “Serbuk Daun Jambu Biji Memperbaiki Performans Pertumbuhan dan Morfologi Duodenum Ayam Jawa Super,” J. Vet., vol. 19, no. 4, pp. 554–562, 2018. [18]     H. Halimatussakdiah, U. Amna, and P. Wahyuningsih, “Preliminary Phytochemical Analysis and Larvicidal Activity of Edible Fern (Diplazium esculentum (Retz.) Sw.) Extract against Culex,” J. Nat., vol. 18, no. 3, pp. 141–146, 2018, doi: 10.24815/jn.v0i0.11335. [19]     R. Ningrum, E. Purwanti, and Sukarsono, “Identifikasi Senyawa Alkaloid dari Batang Karamunting (Rhodomyrtus tomentosa) Sebagai Bahan Ajar Biologi Untuk SMA Kelas X,” J. Pendidik. Biol. Indinesia, vol. 2, no. 3, pp. 231–236, 2016. [20]     A. R. Nasrudin., Wahyono., Mustofa., Saridarti, “Isolasi Senyawa dari Kulit Akar Sengugun (Elerdenrum serratum L. Moon),” J. Ilm. Farm., vol. 6, no. 3, pp. 332–337, 2017. [21]     N. Hidayah, “Pemanfaatan Senyawa Metabolit Sekunder Tanaman (Tanin dan Saponin) dalam Mengurangi Emisi Metan Ternak Ruminansia,” J. Sain Peternak. Indones., vol. 1, no. 2, pp. 89–98, 2016, doi: 10.31186/jspi.id.11.2.89-98. [22]     Z. D. Novitasari, E. A. dan Putri, “Isolasi dan Identifikasi Saponin Saponin Pada Ekstrak Daun Mahkota Dewa Dengan Metode Maserasi,” J. Sains, vol. 6, no. 12, pp. 10–13, 2016. [23]     B. Bintoro, A., Ibrahim, M.A., Situmeang, “Analisis Dan Identifikasi Senyawa Saponin dari Daun Sidara (Zhizipus Mauritania L.),” J. Itekimia, vol. 29, no. 1, pp. 84–93, 2017. [24]     . Y., H. Purnamaningsih, A. Nururrozi, and S. Indarjulianto, “Saponin : Dampak terhadap Ternak (Ulasan),” J. Peternak. Sriwij., vol. 6, no. 2, pp. 79–90, 2017, doi: 10.33230/jps.6.2.2017.5083.

PHYTOCHEMICAL ANALYSIS OF SEED OF MEDICINAL HERB, ARECA CATECHU LINN. AND EVALUATION OF IN-VITRO ANTHELMINTIC ACTIVITY OF ARECOLINE IN AQUEOUS AND ORGANIC EXTRACTS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (02) ◽  
pp. 27-31
Author(s):  
Sumithra M ◽  
Ishwarya N ◽  
Shanmugasundaram P ◽  
Vijeyaanadhi M ◽  
Dineshnath G.
Keyword(s):  
Anthelmintic Activity ◽  
Phytochemical Analysis ◽  
Active Constituent ◽  
Death Time ◽  
Nonpolar Solvents ◽  
Liquid Liquid Extraction ◽  
Areca Catechu ◽  
Organic Extracts ◽  
Anthelmintic Effect

The objective of the study was to extract the active constituent of Areca catechu Linn (Arecaceae) and evaluate the anthelmintic effect of arecoline. liquid liquid extraction was used to extract the arecoline from areca nut. polar and nonpolar solvents were used for extraction. The anthelmintic activity was performed by in vitro studies using adult earthworm (Pheretima posthuma). The anthelminitic test was performed for both extracts and the results compared by using piperazine citrate as reference standard. Water and Hexane there used as polar and non polar solvent for extraction. The hexane extract produced maximum anthelmintic activity with minimum concentration.The hexane and aqueous extracts of nuts of areca catechu exhibited significant anthelmintic activity, as evidenced by decreased paralyzing time and death time. The results support the use of areca catechu as an anthelmintic agent.

scholarly journals In vitro anthelmintic activity of Psidium guajava hydroalcoholic extract against gastro-intestinal sheep nematodes

2020 ◽  
Vol 92 (suppl 2) ◽  
Author(s):  
LUCIANA P. SILVA ◽  
RAFAEL R. DEBIAGE ◽  
JOÃO L. BRONZEL-JÚNIOR ◽  
REGILDO M.G. DA SILVA ◽  
ERIKA C.T. MELLO -PEIXOTO
Keyword(s):  
Anthelmintic Activity ◽  
Psidium Guajava ◽  
Hydroalcoholic Extract

scholarly journals Anthelmintic activity assay of Starchytarpeta jamaicensis L. Vhal tea against Fasciola sp

2021 ◽  
Vol 913 (1) ◽  
pp. 012096
Author(s):  
M R Fahlevi ◽  
I S Pratama ◽  
M Sriasih
Keyword(s):  
Anthelmintic Activity ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Activity Assay ◽  
Control Group Design ◽  
Time Required

Abstract Stachytarpeta jamaicensis L. Vhal (SJ) tea has been empirically used as an anthelmintic, but scientific evidence on its use as an anthelmintic against Fasciola sp. is still limited. The aim of this study was to determine the effective concentration of SJ tea as an anti-Fasciola. S. jamaicensis leaves were cut, dried and then packed in the form of tea bags. The presence of secondary metabolites in the tea was also analyzed phytochemically. The anthelmintic activity assay of SJ tea was carried out using an in vitro experimental design with a post-test with control group design. The in vitro test consisted of negative control (0.9% NaCl), positive control (10% Albendazole) and SJ tea with a concentration of 10%, 5%, and 2.5%. The worm’s movement was observed and the time required for the death of Fasciola was recorded and analyzed. Phytochemical analysis shows that SJ tea contained alkaloid compounds, flavonoids, saponins, tannins and triterpenoids. Time required for death of Fasciola in the positive control, the negative control, the tea concentration of 10%, 5%, and 2.5% were 18.75, 168.75, 23.75, 42.5, and 66.25 min, respectively. S. jamaicensis tea with a concentration of 10% showed comparable effectiveness (p>0.05) to standard reference 10% Albendazole as an anti-Fasciola and resulted in the fastest death of Fasciola fluke compared to other concentrations of the tea. The results of this study indicate that SJ tea can be used as an alternative in overcoming Fasciola infestation. Further works are required to determine its safety when used in vivo.

scholarly journals In vitro anthelmintic activity of Siparuna guianensis extract and essential oil against Strongyloides venezuelensis

2019 ◽  
Vol 94 ◽  
Author(s):  
V.F. Carvalho ◽  
L. dos A. Ramos ◽  
C.A. da Silva ◽  
L. Nebo ◽  
D. Moraes ◽  
...  
Keyword(s):  
Essential Oil ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Phytochemical Analysis ◽  
Egg Hatching ◽  
Aqueous Fraction ◽  
Strongyloides Venezuelensis ◽  
Eggs And Larvae

Abstract New therapeutic approaches are necessary to control strongyloidiasis due to the side effects of, and resistance to, currently available drugs thiabendazole, albendazole, and ivermectin. This study examined the anthelmintic properties of extracts and isolated compounds from Siparuna guianensis against Strongyloides venezuelensis eggs and larvae, using the egg hatching test (EHT) and larval motility test (LMT). Albendazole (0.025 mg/ml) and ivermectin (0.316 mg/ml) were used as the positive controls for the EHT and LMT assays, respectively. Strongyloides venezuelensis eggs or larvae (±50 specimens) were treated with ethanol extract (0.05–1.0 mg/ml), ethyl acetate and aqueous fractions (0.05–0.8 mg/ml), essential oil (0.2–1.0 mg/ml) and α-bisabolol (0.2–1.0 mg/ml) from S. guianensis, and analysed by optical microscopy after 48 h (EHT), or after 24, 48 and 72 h (LMT). All the tested compounds exhibited ovicidal activity equivalent to the positive control and changed the morphology of the eggs. The S. guianensis ethanol extract and aqueous fraction were as effective as the positive control. Phytochemical analysis of the ethanol extract and fractions revealed the presence of phenolic compounds, tannins and flavonoids. Therefore, S. guianensis is effective against S. venezuelensis eggs and larvae in vitro, and can be considered as a potential alternative treatment for strongyloidiasis.

scholarly journals Chemical composition of various plant extracts and their in vitro efficacy in control of Fasciola hepatica eggs

2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Larice Tosi Marques ◽  
Roselena Abreu Guedes ◽  
Winner Duque Rodrigues ◽  
Anderson Barros Archanjo ◽  
Juliana Aparecida Severi ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Fasciola Hepatica ◽  
Tap Water ◽  
Psidium Guajava ◽  
Negative Control ◽  
Southeastern Brazil ◽  
Phytochemical Analysis ◽  
Ovicidal Activity ◽  
Promising Alternative

ABSTRACT: Fasciolosis has been diagnosed in cattle, goats, sheep and horses in southern and southeastern Brazil. Effective alternative treatments are the targets of study. One promising alternative is the use of plant extracts. The aim of this study was to perform phytochemical analysis of extracts of Eugenia uniflora L., Harpagophytum procumbens, Psidium guajava L. and Stryphnodendron adstringens, and to evaluate the in vitro efficacy of these extracts on ovicidal activity in Fasciola hepatica. Plant extracts were analyzed for phytochemical properties. F. hepatica eggs were collected directly from the gallbladders of animals diagnosed as positive for fasciolosis on post mortem examination. One hundred eggs were incubated with 3 ml of each extract at concentrations of 0.10%, 0.25% and 0.50%, albendazole 0.50% (positive control) or tap water (negative control). To determine anti larval efficacy of each plant extract, hatched eggs were counted and the averages were used. Phytochemical analysis revealed the presence of phenolic compounds, tannins and terpenes in most extracts. E. uniflora L. extract was 100% effective at 0.10%, H. procumbens was effective at 0.25% and P. guajava L. and S. adstringens extracts were 100% effective at all concentrations tested. Taken together, the data suggested that ovicidal activity in F. hepatica is due to the presence of these bioactive compounds.

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