Plant Genomic DNA Isolation: An Important Technology for Marker Assisted Selection

2021 ◽  
pp. 85-94
Author(s):  
Sushma Tiwari ◽  
M. K. Tripathi ◽  
R. S. Tomar ◽  
Ashok Ahuja
2012 ◽  
Vol 2 (7) ◽  
pp. 45-46
Author(s):  
Varsha N Nathar ◽  
◽  
Prashant J Gadge

2012 ◽  
Vol 03 (11) ◽  
pp. 1562-1567
Author(s):  
Lingling Zhang ◽  
Yue Pan ◽  
Jinmin Fu ◽  
Junhua Peng

2015 ◽  
pp. 42-42
Author(s):  
Arti Pandey ◽  
Arun Pandey ◽  
Naveen Shreevastava ◽  
Durga Neupane

2016 ◽  
Vol 1 (1) ◽  
pp. 15-27 ◽  
Author(s):  
Kristen A. Leach ◽  
Paula C. McSteen ◽  
David M. Braun

2017 ◽  
Vol 8 (4) ◽  
pp. 045015 ◽  
Author(s):  
Gunajit Goswami ◽  
Himangshu Boruah ◽  
Trishnamoni Gautom ◽  
Dibya Jyoti Hazarika ◽  
Madhumita Barooah ◽  
...  

2013 ◽  
Vol 22 (4) ◽  
pp. 492-497 ◽  
Author(s):  
Lingling Zhang ◽  
Lijing Dai ◽  
Junbo Gou ◽  
Junhua Peng

2008 ◽  
Vol 63 (7-8) ◽  
pp. 595-598 ◽  
Author(s):  
Emel Sözen ◽  
Ismail Poyraz

Origanum onites is an economically important medicinal plant with high essential oil content. Lack of an appropriate DNA isolation procedure is a limiting factor for any molecular study of this plant. We have used a protocol for genomic DNA isolation based on a hexadecyltrimethylammonium bromide (CTAB) method described for other plant species. The method involves mortar grinding of leaf tissue, modified CTAB extraction using high salt concentrations and polyvinyl pyrrolidone, and successive isoamyl alcohol/chloroform extractions. The yield was approx. 20 μg DNA per 200 mg of initial fresh plant material. The genomic DNA obtained by this method was suitable to be used in restriction digests, inter simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) reactions. This extraction method should facilitate the molecular analysis of Origanum chemotypes.


PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109061 ◽  
Author(s):  
Helen E. Barnes ◽  
Guohong Liu ◽  
Christopher Q. Weston ◽  
Paula King ◽  
Long K. Pham ◽  
...  

2005 ◽  
Vol 1064 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Jana Křížová ◽  
Alena Španová ◽  
Bohuslav Rittich ◽  
Daniel Horák

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