scholarly journals Effects of the Aqueous Extract of Cnestis ferruginea Leaves on the Male Reproductive System in Alloxan-induced Diabetic Mice

2021 ◽  
pp. 23-33
Author(s):  
Kouassi Emile Bégbin ◽  
N’Guessan Ernest Zougrou ◽  
Séverin Koffi ◽  
Edwige Alida Odoh ◽  
Koffi Kouakou
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Reproductive System ◽  
Histological Study ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Diabetic Mice ◽  
Sperm Density

Diabetes mellitus is the most common endocrine disease and one of the most common chronic disorders. Diabetes mellitus has been associated with impaired reproductive health, mainly in men. The present study aimed to evaluate effects of the aqueous extract of Cnestis ferruginea leaves on the male reproductive system in alloxan-induced diabetic mice. The determination of phenolic compounds content in the aqueous extract was performed by conventional methods. Diabetes was induced in adult male mice by intraperitoneal injection with a single dose of 220 mg/Kg body weight of alloxan. Animal’s treatment with 100 and 200 mg/Kg of body weight of the aqueous extract was started 34 days after induction of diabetes. Sperm density, morphology and motility were assessed by standard methods. Serum levels of testosterone, FSH, and LH were measured. In addition, the testes were removed for histological study. Proportions of total polyphenols, flavonoids and tannins in the aqueous extract of Cnestis ferruginea were 5260.32 +/- 26 mg EAG /100g, 1384.43 +/- 4 mg EQ /100g and 7380.95 +/- 121 mg EC /100g respectively. 100 and 200 mg/Kg of body weight doses induced highly significant (P ˂ .001) reductions of 68% and 71% in blood glucose levels respectively, significant (P ˂ .05) increases in testes weight of 9% and 10% respectively, highly significant (P ˂ .001) increases in testosterone, pituitary gonadotropins and sperm parameter levels. In addition, regeneration of seminiferous tubules and interstitial cells was observed. Cnestis ferruginea leaves are rich in phenolic compounds. These compounds have anti-diabetic and fertilizing activities on the male reproductive system of diabetic mice.

scholarly journals Effect of Diabetes on the Male Reproductive System—A Histomorphological Study

2019 ◽  
Vol 36 (01) ◽  
pp. 017-023 ◽  
Author(s):  
Sushma Kotian ◽  
Ashok Kumar ◽  
Sanchari Mallik ◽  
Nandini Bhat ◽  
Anne Souza ◽  
...  
Keyword(s):  
Reproductive System ◽  
Histological Study ◽  
Autoimmune Disorder ◽  
Diabetic Rats ◽  
The Body ◽  
Male Reproductive System ◽  
Diabetic Group ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Minimum Diameter

Introduction Type 1 diabetes is an autoimmune disorder characterized by lack of insulin production by the β cells of the pancreas. This lack of insulin causes a variety of systemic effects on the metabolism of the body, one of which is reproductive dysfunction. The present study investigates the effects of diabetes on the male reproductive system of streptozotocin (STZ)-induced diabetic rats. Material and Methods A total of 18 adult male Wistar rats weighing ∼ between 250 and 300 g were included in the present study. The animals were divided into normal and diabetic groups. The diabetic group was further subdivided into 2 subgroups with durations of 24 and 48 days. A single dose of STZ (40 mg/kg body weight) was administrated intraperitoneally to the animals of the diabetic group. After the planned duration, the testes and epididymides were dissected, and their gross weight was measured. The tissues were then processed for histological study. Results The gross weight of the testes and epididymides in diabetic rats at 24 and 48 days showed a decrease in comparison to the control. (p < 0.01 for testes and epididymides).Diabetic animals presented a significant decrease in the diameter of the seminiferous tubules compared with the control group (p < 0.01). The epididymides in the diabetic groups showed a considerable reduction in the tubular surface area compared with the control group (p < 0.01). There was also a reduction in the mean diameter, which was measured using the maximum and minimum diameter of the tubules (p < 0.01). Conclusion The present study is an insight into the adverse effects that diabetes can have on the tissue structure of the testes, of the epididymides, and ultimately on the process of spermatogenesis.

scholarly journals Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

2021 ◽  
Vol 17 (34) ◽  
pp. 149
Author(s):  
Kouassi Emile Bégbin ◽  
N’Guessan Ernest Zougrou ◽  
Georges Abizi ◽  
Koffi Kouakou ◽  
N’Takpé Emmanuel Jaurès Mangué
Keyword(s):  
Diabetes Mellitus ◽  
Adult Male ◽  
Reproductive System ◽  
Male Reproductive System ◽  
Diabetic Group ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Chronic Hyperglycemia ◽  
Study Results

Background: Diabetes mellitus is a disease characterized by chronic hyperglycemia. Poor management of hyperglycemia leads to complications that can reduce quality of life. Diabetes mellitus has been associated with damage to the reproductive system. The present study examines effects of diabetes mellitus on the reproductive system in alloxan-induced diabetic mice after one cycle of spermatogenesis. Materials and Methods: Twelve (12) mice divided into "control" and "diabetic" groups of six (6) animals each were used. Diabetes was induced in adult male mice by intraperitoneal injection with a single dose of 220 mg/kg body weight of alloxan. After 40 days, sperm density, morphology, and motility were assessed by standard methods. Serum levels of testosterone, FSH, and LH were measured. In addition, testes and epididymis were removed for histological study. Results: Highly significant (P < 0.001) reductions in serum testosterone, FSH, and LH levels, as well as normal and motile sperm counts of 21% and 34% respectively were observed in the diabetic group. The control group had three times more sperm than the diabetic group. The histological increased inter-tubular spaces, loss of interstitial tissue, degeneration of seminiferous tubules, and almost complete reduction of sperm count in the epididymal duct. Conclusion: Chronic hyperglycemia is therefore deleterious to the male reproductive system of mice. It leads to hypogonadism, which causes dysfunction of the male reproductive system, and can lead to infertility in men with diabetes mellitus.

Effect of Crude Leaves Extract of Bersama Abyssinica on Blood Glucose Level and Serum Lipid Level of Streptozotocin-Induced Diabetic Mice: Evidence for In vivo Antidiabetic Activity

2021 ◽  
Vol 19 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Agumas Alemu Alehegn ◽  
Baye Yrga Adugna ◽  
Abebe Basazn Mekuria ◽  
Engidaw Fentahun Enyew
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Serum Lipid ◽  
Lipid Level ◽  
Serum Lipid Level ◽  
Diabetic Mice ◽  
Lipid Levels ◽  
Antidiabetic Effect ◽  
Bersama Abyssinica

Background: Diabetes mellitus is one of the major and common metabolic, and chronic disorders in the world. Several medicinal plants have been used globally for the management of diabetes mellitus. The current study aimed to study the anti-hyperglycemic and anti-hyperlipidemic effects of Bersama abyssinica. Methods: Antidiabetic effect of 80% methanolic crude extract of Bersama abyssinica was studied in repeated dose-treated STZ-induced diabetic mice model. The activities of Bersama abyssinica on serum lipid level and body weight were investigated on STZ-induced diabetic mice. Data were analyzed using one-way ANOVA and significant when the p-value was less than 0.05. Results: All doses of the crude 80% methanolic extract of Bersama abyssinica (100 mg/kg, 200 mg/kg, and 400 mg/kg) exhibited a noticeable BGL reduction when compared with baseline blood glucose level and diabetic control on the 7th and 14th days of administration. Moreover, the higher dose of the extract (at 400 mg/kg) significantly (p < 0.001, 54.3%) decreased the BGL in STZ-induced diabetic mice. The maximum decrement in fasting BGL was achieved at the 14th days: 34.92%, 41.10%, 54.30%, and 59.66%, respectively for BAC 100 mg/kg, BAC 200 mg/kg, BAC 400 mg/kg, and GLC 5 mg/kg treated groups. Bersama abyssinica also displayed a significant (p < 0.05) improvement of serum lipid levels and body weight. Conclusion: Bersama abyssinica crude extract exhibited a significant antidiabetic effect and prevented body weight loss in streptozotocin-induced diabetic mice. The finding also confirmed the valuable biochemical activity of Bersama abyssinica by improving serum lipid levels.

Hypoglycemic effects of phenolic compound-rich aqueous extract from water dropwort (Oenanthe javanica DC.) on streptozotocin-induced diabetic mice

2020 ◽  
Vol 44 (14) ◽  
pp. 5190-5200
Author(s):  
Shudong He ◽  
Mingming Tang ◽  
Zuoyong Zhang ◽  
Haiyan Liu ◽  
Mingfeng Luo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Aqueous Extract ◽  
Akt Pathway ◽  
Diabetic Mice ◽  
Glut4 Translocation ◽  
Hypoglycemic Action ◽  
Oenanthe Javanica ◽  
Water Dropwort

Phenolic compounds in water dropwort aqueous extract were identified, and the IRS-2/PI3K-AKT pathway and GLUT4 translocation were regulated for hypoglycemic action.

scholarly journals Red-Fleshed Apple Anthocyanin Extracts Attenuate Male Reproductive System Dysfunction Caused by Busulfan in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Jihua Xu ◽  
Xiang Zhang ◽  
Xiaohong Sun ◽  
Qiang Lv ◽  
Yugang Zhang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Oral Administration ◽  
Reproductive System ◽  
Sperm Concentration ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Tandem Mass ◽  
Treatment Groups ◽  
Positive Effects

In this research, we analyzed the effect of an intragastrical oral administration of red-fleshed apple anthocyanin extract (RAAE) on busulfan-treated mice. First, we showed that the most abundant component in RAAE was cyanidin 3-O-galactoside. To determine the effect of the RAAE, the mice were divided into control and four other different concentrations of RAAE feeding treatment groups (BA0, no RAAE; BA.1, 0.1 mg/kg; BA1, 1 mg/kg; and BA5, 5 mg/kg) following busulfan injection. We observed that RAAE treatments displayed ameliorative effects on male reproductive system dysfunction caused by busulfan, such as recovering the irregular arrangements of seminiferous tubules, increasing the number of spermatogonia and spermatocytes, improving sperm concentration by 3-fold in BA.1, and improving sperm motility by 2-fold in BA1. The liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis showed significant up- or downregulation of certain metabolites, such as lysophosphatidylcholine (LysoPC), L-arginine, glycine, anandamide, and L-carnitine, which could contribute to the positive effects of RAAE, especially in PBA1 (plasma of BA1) and PBA5 (plasma of BA5). Taken together, the results indicate that 1 mg/kg of RAAE is a suitable concentration for rescuing spermatogenesis in mice. The research suggests that RAAE could be a potential nutraceutical for protecting spermatogenesis after busulfan therapy in cancer.

scholarly journals METRONIDAZOLE INDUCES SIGNIFICANT PATHOLOGICAL ALTERATIONS IN THE MALE REPRODUCTIVE SYSTEM OF MICE

2021 ◽  
Vol 52 (6) ◽  
pp. 1375-1381
Author(s):  
Z. K. Al – Timimi
Keyword(s):  
Reproductive System ◽  
Sperm Morphology ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Histopathological Changes ◽  
Negative Effects ◽  
The Mean ◽  
Water Treated Control

The present study was aimed to investigate the possible effects caused by the antibiotic, metronidazole, on the histology of the testis and the morphology of the sperms of mice. To achieve this,  adult male albino Swiss Mice were orally administrated with 0.1 ml (100 mg\ kg ) of the commercially used metronidazole (Flagyl) for 60 consecutive days. The results showed significant decreases (p<0.05) in the mean weight of body and testis in the treated mice as compared to the distilled water-treated control group. The treatment also caused several histopathological changes in the testis which included necrosis, congestion, hemorrhage, edema, germ cell downfall  and rupture in the seminiferous tubules. The tests of the sperm morphology revealed a number of deformations as a result of the treatment, such as the appearance of headless,  bend, broken-tailed, hook-tailed, and two-headed sperms, as well as sperms with cytoplasmic droplets. We conclude that from these results that orally administered Metronidazole exerts negative effects on the male reproductive system of mice .

scholarly journals Potential Protective Effect of Pomegranate (Punica Granatum) Juice on Monosodium Glutamate Induced Seminiferous Tubules Changes in Adult Male Albino Rats: Histological Study

2019 ◽  
Vol 16 (3) ◽  
pp. 625-636
Author(s):  
Aiman Al-Qtaitat ◽  
Sinan S Farhan ◽  
Aiman Al-Maathidy ◽  
Ghadeer Almuhaisen ◽  
Jihad Alzyoud
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Adult Male ◽  
Histological Study ◽  
Monosodium Glutamate ◽  
Pomegranate Juice ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Standard Diet ◽  
Group I

Monosodium glutamate (MSG) has been recognized as flavor enhancer that adversely affects male reproductive systems. The present study was designed to investigate the potential protective effects of pomegranate juice on MSG induced histopathological changes in the seminiferous tubules of rats. Fifty adult male albino rats were divided into five groups of ten rats each; Group I (Control group), received daily standard diet only for one month. Group II (Pomegranate group), received daily pomegranate juice only for one month. Group III (MSG group), received daily a single dose of 60 mg/kg body weight of MSG for one month. Group IV (MSG and Pomegranate group), received daily a single dose of 60 mg/kg of MSG concomitant with pomegranate juice for one month. Group V (MSG withdrawal group), received daily a single dose of 60 mg/kg body weight of MSG for one month then leaved for another one month. The testis was subjected to histological study, using light and electron microscopes, and the cauda epididymis was used for caudal sperm count. Results: MSG induced toxicity in testicular tissues. Pomegranate juice resulted in improving the MSG induced changes, and it had the ability to increase sperms number and to reduce sperms abnormalities. Supplementation of pomegranate juice could ameliorate the MSG induced testicular toxicity. Thus, it could have a role in improving male fertility.

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