scholarly journals Impact of Four Plant Extracts on Bactrocera dorsalis a Pest on Fruits

2021 ◽  
pp. 181-186
Author(s):  
Seema Ramniwas ◽  
Divya Singh
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Larval Mortality ◽  
Bactrocera Dorsalis ◽  
Study Results ◽  
Mentha Longifolia ◽  
Ocimum Tenuiflorum ◽  
Local Plants ◽  
Bougainvillea Glabra ◽  
Third Instar Larvae

Menthol extracts of four local plants (Ocimum tenuiflorum, Hibiscus, Mentha longifolia and Bougainvillea glabra) were analysed to check their toxicity on third instar larvae of B. dorsalis by estimating the larval mortality for four plant extracts and different times exposure (1-5 hr) and measured LT50 value for each plant extract. Larval mortality varies for extract of each studied plant as Ocimum tenuiflorum showed its highest value of 56.68% at 4.57hr, for hibiscus it was 72% at 3.5 hr., for Mentha longifolia it was 95.23% at 4 hr., while for Bougainvillea glabra it was 100% at 2 hr. exposure. The LT50 values for B. dorsalis varying from 1.011 for Bougainvillea glabra to 2.946 for Ocimum tenuiflorum whereas LT50 values were 1.402 and 1.123, forHibiscus and Mentha longifolia respectively. Present study results showed that Bougainvillea glabra was highly toxic whereas Ocimum tenuiflorum shows least toxicity.

scholarly journals Larvicidal Efficacies of Four Indigenous Plant Extracts Against Red Flour Beetle, Tribolium Castaneum (Herbst) (Coleoptera: Tenebrionidae)

2017 ◽  
Vol 43 (2) ◽  
pp. 223-232
Author(s):  
Md Adnan Al Bachchu ◽  
Kismot Ara ◽  
Md Nizam Uddin ◽  
Roushan Ara
Keyword(s):  
Tribolium Castaneum ◽  
Plant Extract ◽  
Plant Extracts ◽  
Larval Mortality ◽  
Red Flour Beetle ◽  
Time Interval ◽  
Leaf Extracts ◽  
Flour Beetle ◽  
Indigenous Plant ◽  
Castor Plant

An investigation was carried out on the larvicidal efficacy of four indigenous plant extracts against the 6th instar larvae of red flour beetle, Tribolium castaneum (Herbst) during the period of February to July 2016. The result revealed that all the four plant extracts were effective in checking insect infestation and had different toxic effects against the 6th instar larvae of red flour beetle. Average mortality indicated that castor plant extract showed the highest toxic effect (average mortality 57.78%) whereas the lowest toxicity (average mortality 27.46%) was found in the neem leaf extracts. The larval mortality significantly differed among all the concentrations of the plant extracts applied and the highest mortality (60.75%) was recorded in maximum concentration (10.61 mg/cm2) of the plant extract. No larval mortality was observed in untreated control up to 72 HATs. Mortality percentage was also observed directly proportional to the level of concentrations of plant extracts and to the exposure period. Probit analysis of larval mortality also revealed that the castor plant extract showed the highest toxicity at different time interval among all the plant extracts applied. Asiat. Soc. Bangladesh, Sci. 43(2): 223-232, December 2017

scholarly journals Removal of Listeria monocytogenes Biofilm with Some Local Plant Extracts of Kurdistan Region

2019 ◽  
Vol 7 (2) ◽  
pp. 1-4
Author(s):  
Srwa A. Mohammed
Keyword(s):  
Listeria Monocytogenes ◽  
Plant Extract ◽  
Plant Extracts ◽  
Antimicrobial Agents ◽  
Punica Granatum ◽  
Kurdistan Region ◽  
Planktonic Cells ◽  
Biofilm Production ◽  
Local Plant ◽  
Local Plants

The results of removing or reducing biofilm were determined using subinhibitory concentrations (SICs) of some local plants in the flora of Kurdistan region of Iraq on biofilm production in Listeria monocytogenes. About 10–500 µg/ml of the plant extracts were used against growth and biofilm production in tissue culture plates which were measured spectroscopically. The SIC effects of each of Eugenia caryophyllata (Clove) and Rhus glabra (Sumac) have significantly affected on planktonic cells and reduced sessile cells or biofilm formation in L. monocytogenes. Remarkably, the data showed strong biofilm reduction under synergistically effect of the plant extract mixed with the antimicrobial agents in multidrugresistant L. monocytogenes. Whereas the SIC some of other plant extracts such as Salix candida and pomegranate (Punica granatum) have significantly enhanced biofilm expression in L. monocytogenes, but the use of these extracts synergistically with some antibiotics also resulted in inhibition of biofilm in comparison to merely use of either the extracts or antibiotics. The result of statistical analyses shows that P > 0.05. The results showed that the combination of plant extract with antibiotic together has more effect than using plant merely.

scholarly journals Effects of plant extracts on antioxidant status and oxidant-induced stress in Caco-2 cells

2007 ◽  
Vol 97 (2) ◽  
pp. 321-328 ◽  
Author(s):  
S. Aisling Aherne ◽  
Joseph P. Kerry ◽  
Nora M. O'Brien
Keyword(s):  
Dna Damage ◽  
Plant Extract ◽  
Plant Extracts ◽  
Antioxidant Status ◽  
Cell Injury ◽  
Membrane Integrity ◽  
Neutral Red ◽  
Dna Integrity ◽  
Cell Membrane Integrity ◽  
Wide Range

Experimental evidence suggests that most herbs and spices possess a wide range of biological and pharmacological activities that may protect tissues against O2-induced damage. The objectives of the present study were: first, to determine the effects of plant extracts on the viability, membrane integrity, antioxidant status and DNA integrity of Caco-2 cells and second, to investigate the cytoprotective and genoprotective effects of these plant extracts against oxidative stress in Caco-2 cells. The plant extracts examined were rosemary (Rosmarinus officinalis L.), oregano (Origanum vulgare L.), sage (Salvia officinalis L.) and echinacea (Echinacea purpurea L.). Cell membrane integrity was assessed by the lactate dehydrogenase release assay. Viability was determined by the neutral red uptake assay (NRUA) and the concentration of compound that resulted in 50 % cell death (IC50) was calculated. Antioxidant status of the cells was assessed by measuring GSH content, catalase activity and superoxide dismutase activity. To examine their cytoprotective and genoprotective effects, Caco-2 cells were pre-treated with each plant extract for 24 h followed by exposure to H2O2. DNA damage was assessed by the comet assay and cell injury was determined by the NRUA. Rosemary was the most toxic (IC50 123 μg/ml) and echinacea the least toxic (IC50 1421 μg/ml). Sage was the only plant extract to affect the antioxidant status of the cells by increasing GSH content. Sage, oregano and rosemary protected against H2O2-induced DNA damage (olive tail moment and percentage tail DNA), whereas protection against H2O2-induced cytotoxicity was afforded by sage only.

scholarly journals Fertigation of ‘BRS Princesa’ banana with humic substance and saponin-based plant extracts

Revista CERES ◽  
2017 ◽  
Vol 64 (4) ◽  
pp. 392-398
Author(s):  
Diego Magalhães de Melo* ◽  
Eugênio Ferreira Coelho ◽  
Raul Castro Carriello Rosa ◽  
Ana Lucia Borges ◽  
Djalma Barbosa dos Santos ◽  
...  
Keyword(s):  
Humic Substances ◽  
Humic Substance ◽  
Plant Extract ◽  
Plant Extracts ◽  
Block Design ◽  
Fulvic Acids ◽  
Growth And Yield ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Banana Cultivar

ABSTRACT The use of humic substances and plant extract has been increasing mainly in organic crop systems without scientific-based recommendations. Research is necessary to evaluate the feasibility and the recommendations of these substances. The objective of this work was to evaluate the effect of humic substances (humic and fulvic acids) applied by fertigation with and without saponin-based plant extracts on growth and yield of ‘BRS Princesa’ banana. The experiment was carried out using the banana cultivar ‘BRS Princesa’, at a spacing of 2.0 × 2.5 m, fertigated by drip irrigation in a Dystric Densic Xantic Ferralsol, at the Recôncavo da Bahia, Brazil. Treatments consisted of five doses of humic substances (0.0; 70; 105; 140, and 210 L ha-1cycle-1) applied with and without a saponin-based plant extract in a randomized block design in split-plot scheme. The following growth variables were evaluated: number of leaves, pseudostem height, pseudostem diameter, and length and width of the third leaf. The number of fruits/hand and hands/bunch, hand and bunch yield, and length and fruit diameter of the second central hand were evaluated during harvest. The doses of humic substances influenced only pseudostem height. Hand yield, as well as fruit length and diameter of ‘BRS Princesa’ banana were higher in plants fertigated with humic substance and plant extract in comparison with plants fertigated with only humic substance.

scholarly journals Phytochemical analysis and antibacterial activity of Staurogyne sp. in fish disease bacteria

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Media Fitri Isma Nugraha ◽  
Hessy Novita ◽  
Muh Alias Rajamuddin ◽  
Rossa Yunita ◽  
Wening Enggarini ◽  
...  
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Streptococcus Agalactiae ◽  
Aquatic Plant ◽  
Chromobacterium Violaceum ◽  
Edwardsiella Ictaluri ◽  
Growth Media ◽  
Phytochemical Analysis ◽  
Fish Disease ◽  
Flavobacterium Columnare

This study was aimed to identify the potential use of active compounds extracted from Staurogyne sp as an antibacterial agent to control disease-causing bacteria in fish. Staurogyne sp, an aquatic plant collected from Bantimurung, South Sulawesi were subjected to allelochemical compound. Plant extracts were tested to five bacteria including four types of bacterial pathogen, such as Aeromonas hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, and Flavobacterium columnare, and one bacterial sensing quoroum, Chromobacterium violaceum. Based on phytochemical analysis, Staurogyne’s extracts derived from both stem and root contained flavonoids, phenols, and antioxidants compounds with the concentration of 0.018 mgQE/g, 0.3471 mgGAE/g, and  1004,391 IC50 µg/mL, respectively. In addition, Staurogyne plant extracts derived from both their stem and root has also produced both saponins and glycosides compounds. Plant extracts derived from Staurogyne’s leaves revealed three major chemical compounds such as flavonoids (0.77 mgQE/g), phenol (0.0629 mgGAE/g), and glycosides (+). However, no antioxidants and saponin compounds were detected.  Applying plant extracts as an antibacterial on five disease-causing bacteria causing diseases in aquaculture, such as Aeromonas Hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, Flavobacterium columnare, and Chromobacterium violaceum bacterials revealed that at concentration of 0.1 g the Staurogyne sp extracts did not influence all the bacterials growth. However, by application of plant extract at concentration of 0.2 g on the growth media,three out of 5 tested bacterials (Aeromonas hydrophilla, Edwardsiella ictaluri, and Streptococcus agalactiae) showed intermediate inhibition responses on bacterial growth. The growth of remaining two bacterials pathogen, Flavobacterium columnare and Chromobacterium violaceum at 0.2 g plant extract of Staurogyne sp was not affected. This study revealed that the use of active compound derived from Staurogyne sp would be potential to be used in inhibiting disease-causing bacterial in fish in future. However, optimum concentration of the plant extracts, in particular on the inhibition of the growth of disease-causing bacteria in fish is still needed to adjust.Key words: Antibacterial, aquatic plant, Staurogyne sp, fish disease

Antimicrobial effects of plant extracts against Clostridium perfringens with respect to food-relevant influencing factors

2021 ◽  
Author(s):  
Ulrike Friedlein ◽  
Samart Dorn-In ◽  
Karin Schwaiger
Keyword(s):  
Influencing Factors ◽  
Plant Extract ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Screening Method ◽  
Extrinsic Factors ◽  
Antimicrobial Effects ◽  
Extract Concentration ◽  
Microdilution Method

The application of plant extracts (PEs) could be a promising option to satisfy consumers’ demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of ten hydrophilic plant extracts against two strains of C. perfringens facing various food-relevant influencing factors. Due to the high opacity of PEs, resazurin was used as an indicator for bacterial growth instead of pellet formation. The highest value of the minimum inhibitory concentration (MIC) of the replications of each PE was defined as effective plant extract concentration (EPC), whereas the next concentration beneath the lowest MIC value was defined as the ineffective plant extract concentration (IEPC). The EPC of seven PEs: allspice, cardamom, cinnamon, clove, coriander, ginger and mace were between 0.625 - 10 g/kg, whereas extracts of caravey, nutmeg and thyme showed no antimicrobial activity up to the maximum concentration tested (10 g/kg) against C. perfringens in vitro. Two intrinsic factors, sodium chloride and sodium nitrite, displayed either synergistic/additive effects or no interaction with most PEs. By combination with PEs at its ineffective plant concentration (IEPC, 0.08 – 1.25 g/kg), MIC of NaCl and NaNO2 decreased from 25 – 50 g/kg to 6 – 25 g/kg and > 200 mg/kg to 0.2 – 100 mg/kg respectively. On the contrary, lipid (sun flower oil) at a low concentration inhibited the antimicrobial effects of all tested PEs. For extrinsic factors, only allspice, ginger and coriander could maintain their antimicrobial effects after being heated to 78 °C for 30 min. The synergistic effect between PEs and pH values (5.0 and 5.5) was also found for all PEs. The established screening method with resazurin and defining EPC and IEPC values allows the verification of antimicrobial effects of PEs under various food-relevant influencing factors in a fast and reproducible way.

scholarly journals Effects of Different Plant Extracts on Post-Thaw some Spermatological Parameters of Cryopreserved Awassi Rams Spermatozoa

2018 ◽  
Vol 75 (1) ◽  
pp. 49
Author(s):  
Ilker YAVAS ◽  
Tugba KORKMAZ YAVAS
Keyword(s):  
Citric Acid ◽  
Plant Extract ◽  
Plant Extracts ◽  
Artificial Insemination ◽  
Egg Yolk ◽  
Echinacea Purpurea ◽  
Final Concentration ◽  
Rosmarinus Officinalis ◽  
Rosemary Extract ◽  
Positive Effects

Several diluting–protecting media and different methods have been used for storage of ram semen in liquid and frozen state (Salamonand Maxwel, 2000). Addition of preservatives media for increasing storage of sperm are very important for successful artificial insemination in rams. This study was conducted on evaluate the effect of 3 plant extract. Ejaculates were collected using an electro-ejaculator twice a week during there productive season. After pooling, each pooled ejaculate was split into four equal aliquots and diluted with based Tris, Citric acid, Egg yolk, glicerol extender containing plant extracts (0.02% and % 0.04 Rosemary (Rosmarinus officinalis) (Rosemary extract -oil-soluble)); Echinacea (Echinacea purpurea) (0.5 mg/mL and 1 mg/ml) and St. John’s wort (Herba Hyperici) (0.5 mg/mL and 1 mg/mL) at a final concentration of approximately 2x108 motil spermatozoa per straw. These results indicate that that the addition of Echinaceaand Rosemary as antioxidants have more positive effects for Awassi rams on spermatological parameters.

Synthesis of complementary RNA by RNA-dependent RNA polymerases in plant extracts is independent of an RNA primer

2008 ◽  
Vol 35 (11) ◽  
pp. 1091 ◽  
Author(s):  
Lei Wang ◽  
Neil A. Smith ◽  
Lan Zhang ◽  
Elizabeth S. Dennis ◽  
Peter M. Waterhouse ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Rna Polymerase ◽  
Plant Extract ◽  
Plant Extracts ◽  
Callus Tissue ◽  
Polymerase Activity ◽  
Loss Of Function ◽  
Rna Dependent Rna Polymerase ◽  
Methyl Group ◽  
The Individual

RNA-dependent RNA polymerase (RDR) activities were readily detected in extracts from cauliflower and broccoli florets, Arabidopsis thaliana (L.) Heynh callus tissue and broccoli nuclei. The synthesis of complementary RNA (cRNA) was independent of a RNA primer, whether or not the primer contained a 3′ terminal 2′-O-methyl group or was phosphorylated at the 5′ terminus. cRNA synthesis in plant extracts was not affected by loss-of-function mutations in the DICER-LIKE (DCL) proteins DCL2, DCL3, and DCL4, indicating that RDRs function independently of these DCL proteins. A loss-of-function mutation in RDR1, RDR2 or RDR6 did not significantly reduce the amount of cRNA synthesis. This indicates that these RDRs did not account for the bulk RDR activities in plant extracts, and suggest that either the individual RDRs each contribute a fraction of polymerase activity or another RDR(s) is predominant in the plant extract.

scholarly journals Bioactivity of Common Pesticidal Plants on Fall Armyworm Larvae (Spodoptera frugiperda)

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 112 ◽  
Author(s):  
Kelita Phambala ◽  
Yolice Tembo ◽  
Trust Kasambala ◽  
Vernon H. Kabambe ◽  
Philip C. Stevenson ◽  
...  
Keyword(s):  
Pest Management ◽  
Plant Species ◽  
Plant Extracts ◽  
Spodoptera Frugiperda ◽  
Larval Mortality ◽  
Fall Armyworm ◽  
Invasive Pest ◽  
Toxicity Tests ◽  
Contact Toxicity ◽  
Foliar Damage

The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae) is a recent invasive pest species that has successfully established across sub-Saharan Africa where it continues to disrupt agriculture, particularly smallholder cereal production. Management of FAW in its native range in the Americas has led to the development of resistance to many commercial pesticides before its arrival in Africa. Pesticide use may therefore be ineffective for FAW control in Africa, so new and more sustainable approaches to pest management are required that can help reduce the impact of this exotic pest. Pesticidal plants provide an effective and established approach to pest management in African smallholder farming and recent research has shown that their use can be cost-beneficial and sustainable. In order to optimize the use of botanical extracts for FAW control, we initially screened ten commonly used plant species. In laboratory trials, contact toxicity and feeding bioassays showed differential effects. Some plant species had little to no effect when compared to untreated controls; thus, only the five most promising plant species were selected for more detailed study. In contact toxicity tests, the highest larval mortality was obtained from Nicotiana tabacum (66%) and Lippia javanica (66%). Similarly, in a feeding bioassay L. javanica (62%) and N. tabacum (60%) exhibited high larval mortality at the highest concentration evaluated (10% w/v). Feeding deterrence was evaluated using glass-fibre discs treated with plant extracts, which showed that Cymbopogon citratus (36%) and Azadirachta indica (20%) were the most potent feeding deterrents among the pesticidal plants evaluated. In a screenhouse experiment where living maize plants infested with fall armyworm larvae were treated with plant extracts, N. tabacum and L. javanica were the most potent species at reducing foliar damage compared to the untreated control whilst the synthetic pesticide chlorpyrifos was the most effective in reducing fall armyworm foliar damage. Further field trial evaluation is recommended, particularly involving smallholder maize fields to assess effectiveness across a range of contexts.

scholarly journals Redox Status Is Critical for Stemness in Skin Equivalents

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hye-Ryung Choi ◽  
Youn-A Kang ◽  
Jung-Won Shin ◽  
Jung-Im Na ◽  
Chang-Hun Huh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Vitamin C ◽  
Plant Extract ◽  
Plant Extracts ◽  
Redox Status ◽  
Proliferative Potential ◽  
Basal Cells ◽  
Intense Staining ◽  
Significant Difference

The skin is constantly exposed to environmental oxidative stress. Skin equivalent (SE) models are three-dimensional systems in which cell-cell or cell-matrix interactions can be investigated. In this study, the effects of vitamin C or plant extracts with high antioxidant activities were tested. There was no significant difference in the epidermal thickness, but the basal cells became cuboidal when vitamin C or plant extracts were supplemented. Furthermore, immunohistochemical staining showed linear and intense staining ofα6 andβ1 integrin along the basement membrane in vitamin C or plant extract treated models. The p63 and PCNA were also stained. Results showed that the number of p63 and PCNA positive cells was higher in the vitamin C or plant extract treated models than in the control SEs. Although the relationship between oxidative stress and stem cells is not known, our results suggest that redox status affects the stemness and the proliferative potential of epidermal basal cells by modulating microenvironment to epidermal basal stem cells.

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