Gas chromatographic analysis of the fatty acid composition of mustard oil obtained by cold pressing (method)

2020 ◽  
pp. 391
Author(s):  
Alexander Nikolaevich Ostrikov ◽  
Natalya Leonidovna Kleymenova ◽  
Inessa Nikolaevna Bolgova ◽  
Maxim Vasilyevich Kopylov
Keyword(s):  
Fatty Acids ◽  
Composition Studies ◽  
Test Sample ◽  
Component Composition ◽  
Mustard Oil ◽  
Gas Liquid Chromatography ◽  
Pressing Method ◽  
Cold Pressing ◽  
Annular Gap ◽  
Β Carotene

Nowadays there is such a type of product as mustard oil, which is a stockpot of valuable fatty acids and vitamins. Mustard oil is used in various fields of industry. It has a characteristic smell and taste, yellow-brown color. Mustard grows mostly in the southern regions. It is resistant to various diseases and pests. There is evidence from scientific research that proves the beneficial properties of this oilseed. Mustard oil was obtained by cold pressing under the following conditions: annular gap of the grain chamber of the oil press was 0.6 mm; screw rotation speed was 166 rpm; pressing temperature was 345 K. The study of the component composition of mustard oil was carried out by gas-liquid chromatography. The qualitative composition of the test sample was determined in the work. It contains the following acids - palmitic (4%), stearic (3%), polyunsaturated acids - linoleic (32%), linolenic (8%) and eicosenic (2%), monounsaturated acid - oleic (47%). The data obtained correlate with literary data. It was proved that unsaturated acids can act as essential nutrients. The presence of other fatty acids, the amount of which is within acceptable limits, is also identified in the work. Mustard oil vitamin composition studies were carried out according to standard methods. Vitamins A, E, K, β-carotene and α, β + γ, δ tocopherols were found in the original sample. Vitamins A, E, K increase immunity and strengthen the walls of blood vessels, as well as are anti-inflammatory substances. The presence of tocopherols benefits the skin. The results of studies of mustard oil allow us to conclude that the components identification is necessary for the development of balanced blends with a reduced amount of saturated acids and a high amount of semi-saturated acids.

scholarly journals Research of the process of receiving and preparing for to use of ecologically pure biofuel on the basis of tall oil

2020 ◽  
Vol 24 (5) ◽  
pp. 97-103
Author(s):  
A.N. Ivankin ◽  
◽  
A.N. Zarubinа ◽  
G.L. Oliferenko ◽  
A.S. Kuleznev ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Raw Materials ◽  
Methyl Esters ◽  
General Scheme ◽  
Total Content ◽  
Component Composition ◽  
Gas Liquid Chromatography ◽  
Organic Substances ◽  
Tall Oil

The article discusses the scientific and technical issues of the methodology for producing liquid biofuel from renewable raw materials of plant origin. As raw materials used wastes from the production of pulp and paper mills — tall oil. The purpose of the work was to study the process of obtaining and determining ways to use the product. The optimal conditions for obtaining biodiesel are determined. The processing of raw materials was carried out by heating it with methanol in a ratio of 1:0,3 at a temperature of 50–65 °C for 2 hours in the presence of 2 % catalyst. The product, after separation of the resulting intermediate, was finally washed with water from the catalyst residues. The process was controlled spectrophotometrically. Using gas-liquid chromatography with mass spectrometric detection, the complete chemical composition of the used raw materials of various degrees of purification was established. It is shown that the main content in the processed oil is represented by natural lipids. Their fatty acid composition is described, comprising more than thirty C10–C24 fatty acids. A general scheme for producing biodiesel, which is a mixture of fatty acid alkyl esters, has been developed. The product was obtained by chemical transformation in the presence of acid catalysts, followed by the formation of fatty acid methyl esters. The component composition of the obtained product, biodiesel, was studied and it was shown that it consists of a mixture of methyl esters of fatty acids more than 95 %. In biodiesel, more than two hundred organic substances are also contained in the form of an insignificant amount of microimpurities. Their number fluctuated around 0,001 %. The basic physico-chemical characteristics of the obtained biodiesel are described in comparison with international requirements for biofuels. The analyzed product samples obtained from distilled TM, according to mass spectrometry, did not contain harmful impurities bromine, iodine, phosphorus and sulfur-substituted compounds. The total content of chlorine-substituted organic substances in all samples did not exceed 0,07 ± 0,02 %, and N-substituted derivatives did not exceed 0,05 ± 0,01 %, which indicates a rather high ecological purity of bitumen fuel. The main directions of the possible use of the product as liquid fuel for mini-boiler nozzles, as well as for operation in conventional diesel engines, are determined.

scholarly journals Study of the Qualitative Characteristics of Rapeseed Oil Obtained by Cold Pressing

2021 ◽  
Author(s):  
Natalia L Kleymenova ◽  
Inessa N Bolgova ◽  
Maxim V Kopylov ◽  
Ekaterina Y Zheltoukhova
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Rapeseed Oil ◽  
Gas Liquid Chromatography ◽  
Oil Composition ◽  
Cold Pressing ◽  
Metabolism Regulation ◽  
Annular Gap ◽  
B Vitamin

Oil from spring rape seeds obtained by cold pressing was selected as the object of this study. Oil samples were obtained under the following technological conditions: the annular gap was 0.7 mm in a grain chamber and the screw rotation speed was 160 rpm at a pressing temperature of 315 K. The oil composition was studied by gas-liquid chromatography on the Chromotech 5000 apparatus. Rapeseed oil characteristics were obtained with chromatograms. An increased content of linoleic acid 20% (ω6) and linolenic acid 12% (ω3) was revealed in the fatty acid composition of the sample. Fatty acids contribute to lipid metabolism regulation. They are of primary importance in the diet. The presence of 58.6% oleic acid (ω9) was identified. The resulting rapeseed oil had healing properties due to the presence of ω3, ω6 and ω9. Vitamin B, vitamin K, traces of vitamin A, and α, β + γ, δ-tocopherols were found. α, β + γ-tocopherols contribute to the oxidation process. According to these results, rapeseed oil can be confidently recommended for introduction into various food recipes. Keywords: rapeseed oil, vitamins, fatty acid composition

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

scholarly journals NMR Metabolite Profiles in Male Meat-Eaters, Fish-Eaters, Vegetarians and Vegans, and Comparison with MS Metabolite Profiles

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 121
Author(s):  
Julie A. Schmidt ◽  
Georgina K. Fensom ◽  
Sabina Rinaldi ◽  
Augustin Scalbert ◽  
Marc J. Gunter ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Clinical Chemistry ◽  
Saturated Fatty Acids ◽  
Density Lipoprotein ◽  
Diet Group ◽  
Gas Liquid Chromatography ◽  
Very Low Density Lipoproteins ◽  
Animal Products ◽  
Total N ◽  
Metabolite Profiles

Metabolomics may help to elucidate mechanisms underlying diet-disease relationships and identify novel risk factors for disease. To inform the design and interpretation of such research, evidence on diet-metabolite associations and cross-assay comparisons is needed. We aimed to compare nuclear magnetic resonance (NMR) metabolite profiles between meat-eaters, fish-eaters, vegetarians and vegans, and to compare NMR measurements to those from mass spectrometry (MS), clinical chemistry and capillary gas-liquid chromatography (GC). We quantified 207 serum NMR metabolite measures in 286 male participants of the European Prospective Investigation into Cancer and Nutrition (EPIC)-Oxford cohort. Using univariate and multivariate analyses, we found that metabolite profiles varied by diet group, especially for vegans; the main differences compared to meat-eaters were lower levels of docosahexaenoic acid, total n-3 and saturated fatty acids, cholesterol and triglycerides in very-low-density lipoproteins, various lipid factions in high-density lipoprotein, sphingomyelins, tyrosine and creatinine, and higher levels of linoleic acid, total n-6, polyunsaturated fatty acids and alanine. Levels in fish-eaters and vegetarians differed by metabolite measure. Concentrations of 13 metabolites measured using both NMR and MS, clinical chemistry or GC were mostly similar. In summary, vegans’ metabolite profiles were markedly different to those of men consuming animal products. The studied metabolomics platforms are complementary, with limited overlap between metabolite classes.

PSIII-12 In vitro Evaluation of Purified Fiber Sources for Production of Short-chain Fatty Acids Using Pig Cecal Content as an Inoculum

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 177-177
Author(s):  
Gabriela E Martinez Padilla ◽  
Rajesh Jha ◽  
Vivek Fellner ◽  
Eric van Heugten
Keyword(s):  
Fatty Acids ◽  
Enzymatic Hydrolysis ◽  
Resistant Starch ◽  
Potato Starch ◽  
Short Chain ◽  
Gas Liquid Chromatography ◽  
Citrus Peel ◽  
Starch Potato ◽  
Chain Fatty Acids

Abstract This study evaluated short-chain fatty acid (SCFA) production from purified fiber sources when fermented in vitro using pig cecal contents as an inoculum. Fiber sources of interest were inulin from chicory root (native and long-chain inulin with 90 and 98% fiber, respectively), pectin from citrus peel (high methoxyl pectin), resistant starch (native starch), potato starch (commercial grade), and β-glucan (β-1,3;β-1,6 yeast-derived). Cellulose and cornstarch were used as indigestible and highly digestible carbohydrates, respectively. Triplicate samples of substrates (2 g) were subjected to enzymatic hydrolysis with pepsin and pancreatin for 6 h. Subsequently, hydrolyzed residues (200 mg) were incubated under anaerobic conditions at 39°C with 30 mL solution of cecal inoculum collected from 3 sows fed a standard commercial diet and buffered mineral solution. After 48 h of incubation, solutions from fermented samples were analyzed for pH, SCFA, and branched-chain fatty acids (BCFA) using gas-liquid chromatography. Enzymatic hydrolysis had no effect on digestion of β-glucan, but total SCFA concentration after fermentation was highest (26.13 mmol/g) followed by resistant starch (22.61 mmol/g) and potato starch (22.20 mmol/g) and was lowest for cellulose (13.91 mmol/g). In contrast, native inulin was highly digested during enzymatic hydrolysis, resulting in the lowest substrate available for fermentation (11.84% DM) and the highest pH (5.98). Enzymatic hydrolysis and fermentation of resistant starch increased (P< 0.001) concentrations of acetate (0.60 mg/g), whereas potato starch and β-glucan yielded more butyrate (0.60 and 0.54 mg/g respectively), and β-glucan resulted in greater (P< 0.001) propionate concentrations (0.69 mg/g). Pectin resulted in the highest fermentation (82.38% DM disappearance) and the lowest pH (4.03) compared to the other fiber sources (P< 0.001) and yielded the lowest BCFA concentration (1.89 mM, P< 0.001). Results suggest that fermentation of resistant starch, potato starch, and β-glucan produced higher SCFA concentrations, while pectin resulted in a decreased pH of fermentation solution.

Quantitative Changes of the Lipid and Fatty Acid Composition of Leaves of Aleurites montana as a Consequence of Growth under 700 ppm CO2 in the Atmosphere

1996 ◽  
Vol 51 (11-12) ◽  
pp. 833-840 ◽  
Author(s):  
P He ◽  
A Radunz ◽  
K. P Bader ◽  
G. H Schmid
Keyword(s):  
Fatty Acids ◽  
Thylakoid Membrane ◽  
Saturated Fatty Acids ◽  
Phospholipid Fraction ◽  
Phospholipid Content ◽  
The Core ◽  
Glycolipid Fraction ◽  
Carbonic Acids ◽  
And Control ◽  
Β Carotene

Abstract Leaf lipids of Aleurites plants that were cultivated for 5 months in air containing 700 ppm CO2, were compared to those of control plants cultivated at 350 ppm CO2. The content of ether soluble lipids referred to dry matter is the same in CO2-and control plants. The comparison of lipids analyzed as the pigments chlorophyll and carotenoids, phospholipids and glycolipids shows that the ratio of phospholipids and glycolipids is slightly shifted in favor of phospholipids in CO2-plants. Thus, within the group of phospholipids, phosphatidylglycerol and phosphatidylinositol occur in higher concentrations in CO2-plants. Although the differences in the lipid content appear moderate in CO2-and control plants, it is the saturation degree of fatty acids that differs substantially. The fatty acids of CO2-plants contain according to the higher phospholipid content approx. 5% more saturated fatty acids. Stearic acid is three-fold increased. Whereas in the phospholipid fraction saturated fatty acids comprise one half of all fatty acids, the unsaturated fatty acids make up for 80 to 90% in the glycolipid fraction. In CO2-plants not only in the phospholipid fraction but also in the glycolipid fraction saturated fatty acids occur in a higher portion. This means that not only in the cell membrane of CO2-plants but also in the thylakoid membrane the fluidity is decreased. Also in the wax-fraction long-chained carbonic acids with 20 -26 carbon atoms occur. As the portion of these carbonic acids is twice as high in CO2-plants, it is concluded that a stronger formation of the wax layers exists in CO2-plants. By means of Western blotting and by the use of lipid and carotenoid antisera the binding of lipids onto proteins of photosystem II and photosystem I was analyzed. It is seen that besides the major amount of lipids which build up the thylakoid membrane, some lipids are also bound to membrane peptides. Whereas monogalactolipid is bound to the LHCP-complex peptides, to the OEC1 -peptide and the 43 and 47 kDa chlorophyll binding peptides, the anionic lipids sulfoquinovosyldiglyceride and phosphatidylglycerol and digalactolipid are bound to the core peptides of PS II and PS I. β-carotene and the xanthophylls were found to be bound to the core peptides and β-carotene and violaxanthin were also bound to the light-harvesting pigment complex.

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