Assessing rates of parasite coinfection and spatiotemporal strain variation via metabarcoding: insights for the conservation of European Turtle Doves Streptopelia turtur

Understanding the frequency, spatiotemporal dynamics and impacts of parasite coinfections is fundamental to developing control measures and predicting disease impacts. The European turtle dove (Streptopelia turtur) is one of Europe’s most threatened bird species. High prevalence of infection by the protozoan parasite Trichomonas gallinae has previously been identified, but the role of this and other coinfecting parasites in turtle dove declines remains unclear. Using a high-throughput sequencing approach, we identified seven strains of T. gallinae, including two novel strains, from ITS1/5.8S/ITS2 ribosomal sequences in turtle doves on breeding and wintering grounds, with further intra-strain variation and four novel sub-types revealed by the iron-hydrogenase gene. High spatiotemporal turnover was observed in T. gallinae strain composition, and infection was prevalent in all populations (89–100%). Coinfection by multiple Trichomonas strains was rarer than expected (1% observed compared to 38.6% expected), suggesting either within-host competition, or high mortality of coinfected individuals. In contrast, coinfection by multiple haemosporidians was common (43%), as was coinfection by haemosporidians and T. gallinae (90%), with positive associations between strains of T. gallinae and Leucocytozoon suggesting a mechanism such as parasite-induced immune modulation. We found no evidence for negative associations between coinfections and host body condition. We suggest that longitudinal studies involving the recapture and investigation of infection status of individuals over their lifespan are crucial to understand the epidemiology of coinfections in natural populations.

The strain variation during the isothermal B2→B19’ martensitic transformation in the quenched or annealed Ni51Ti49 alloy

The strain variation during the isothermal holding under constant stress was studied in the quenched or annealed Ni51Ti49 alloy samples. The isothermal strain variation was found in both samples and this strain was completely recovered on subsequent unloading and heating. This allowed to conclude that the strain variation on holding was caused by the isothermal martensitic transformation. It was found that the maximum value of isothermal strain depended on the alloy heat treatment. This value was equal to 0.5 % in annealed sample and it was equal to 6 % in quenched sample. It was assumed that the formation of the Ni4Ti3 phase during annealing led to a decrease in concentration of substitutional Ni atoms in NiTi phase that were responsible for the isothermal transformation. As a result, the less volume fraction of the martensite formed during holding that supresses the strain variation in annealed samples.

Influence of annealing on the functional properties of the NiTi alloy produced by wire arc additive manufacturing

The influence of the annealing temperature on the recoverable strain variation on cooling and heating under a stress of 200 MPa was studied in the NiTi samples produced by wire arc additive manufacturing. The samples including the Ni-rich NiTi layer in the working length were annealed for 10 hours at various temperature from 450 to 600 °C. It is shown that an increase in annealing temperature leads to non-monontonic variation in the recoverable strain. This is caused by an increase in annealing temperature from 450 to 550 °C increases the volume fraction of Ni4Ti3 precipitates. As a result, the volume fraction of the NiTi phase undergoing the martensitic transformation and recoverable strain decrease. An increase in annealing temperature from 550 to 600 °C leads to a dissolving the Ni4Ti3 precipitates and formation of the Ni3Ti2 precipitates that increases the volume fraction of the NiTi phase and the recoverable strain.

Strain variation in the Ti40,7Hf9,5Ni41,8Cu8 alloy during isothermal martensitic transformation under a constant stress

Strain variation on holding under a constant stress was studied in the Ti40,7Hf9,5Ni41,8Cu8 alloy. It was found, that on holding under stress, the isothermal strain rose up to saturation, which value depended on holding temperature and stress. It was found that the dependencies of the isothermal strain on the holding temperature and stress were non-monotonic. This allowed to find the optimal value of stress and time at which the isothermal strain attained the maximum value of 3.2 %. It was found that the maximum isothermal strain in the Ti40,7Hf9,5Ni41,8Cu8 alloy was less than in the Ti40,7Hf9,5Ni44,8Cu5 alloy.

The Influence of Heel Height on Strain Variation of Plantar Fascia During High Heel Shoes Walking-Combined Musculoskeletal Modeling and Finite Element Analysis

The therapeutic benefit of high heel shoes (HHS) for plantar fasciitis treatment is controversial. It has been suggested that plantar fascia strain can be decreased by heel elevation of shoes which helps in body weight redistribution throughout the length of the foot. Yet it is a fact that the repetitive tension caused by HHS wearing resulting in plantar fasciitis is a high-risk disease in HHS individuals who suffer heel and plantar pain. To explore the biomechanical function on plantar fascia under HHS conditions, in this study, musculoskeletal modeling (MsM) and finite element method (FEM) were used to investigate the effect of heel height on strain distribution of plantar fascia. Three-dimensional (3D) and one-dimensional (1D) finite element models of plantar fascia were generated to analyze the computed strain variation in 3-, 5-, and 7-cm heel heights. For validation, the computed foot contact pressure was compared with experimental measurement, and the strain value on 1D fascia was compared with previous studies. Results showed that the peak strain of plantar fascia was progressively increased on both 3D and 1D plantar fascia as heel elevated from 3 to 7 cm, and the maximum strain of plantar fascia occurs near the heel pain site at second peak stance. The 3D fascia model predicted a higher strain magnitude than that of 1D and provided a more reliable strain distribution on the plantar fascia. It is concluded that HHS with narrow heel support could pose a high risk on plantar fasciitis development, rather than reducing symptoms. Therefore, the heel elevation as a treatment recommendation for plantar fasciitis is questionable. Further studies of different heel support structures of shoes to quantify the effectiveness of heel elevation on the load-bearing mechanism of plantar fascia are recommended.

Thermoresistance in black yeasts is associated with halosensitivity and HPP tolerance, but not with UV tolerance or sanitizer tolerance

Black yeasts can survive extreme conditions in food production owing to their polyextremotolerant character. However, significant strain-to-strain variation in black yeast thermoresistance has been observed. In this study, we assessed the variability in tolerance to nonthermal interventions among a collection of food-related black yeast strains. Variation in tolerance to UV light treatment, high pressure processing, sanitizers, and osmotic pressure was observed within each species. The two strains previously shown to possess high thermotolerance, Exophiala phaeomuriformis FSL-E2-0572 and Exophiala dermatitidis YB-734, were also the most HPP tolerant, but were the least halotolerant. Meanwhile, Aureobasidium pullulans FSL-E2-0290 was the most UV and sanitizer tolerant, but had been shown to have relatively low thermoresistance. Fisher’s exact tests showed that thermoresistance in black yeasts was associated with HPP tolerance and inversely with halotolerance, but no association was found with UV tolerance or sanitizer tolerance. Collectively, the relative stress tolerance among strains varied across interventions. Given this variation, a broad range of different food products are susceptible to black yeast spoilage. Additionally, different strains should be selected in challenge studies specific to the intervention. (1312/2000 characters)

Horizontal gene transfer-mediated bacterial strain variation affects host fitness in Drosophila

Background How microbes affect host fitness and environmental adaptation has become a fundamental research question in evolutionary biology. To better understand the role of microbial genomic variation for host fitness, we tested for associations of bacterial genomic variation and Drosophila melanogaster offspring number in a microbial Genome Wide Association Study (GWAS). Results We performed a microbial GWAS, leveraging strain variation in the genus Gluconobacter, a genus of bacteria that are commonly associated with Drosophila under natural conditions. We pinpoint the thiamine biosynthesis pathway (TBP) as contributing to differences in fitness conferred to the fly host. While an effect of thiamine on fly development has been described, we show that strain variation in TBP between bacterial isolates from wild-caught D. melanogaster contributes to variation in offspring production by the host. By tracing the evolutionary history of TBP genes in Gluconobacter, we find that TBP genes were most likely lost and reacquired by horizontal gene transfer (HGT). Conclusion Our study emphasizes the importance of strain variation and highlights that HGT can add to microbiome flexibility and potentially to host adaptation.

Impact of Strain Variation of Dichelobacter nodosus on Disease Severity and Presence in Sheep Flocks in England

AprV2 and aprB2 are variants of the apr gene of Dichelobacter nodosus, the cause of footrot in sheep. They are putative markers for severe and mild disease expression. The aim of our study was to investigate the distribution of aprV2 and aprB2 in flocks with and without footrot. Our hypotheses were that both strains are present in endemically affected flocks, with aprB2 and aprV2 associated with mild and virulent phenotypes respectively but that D. nodosus is not present in flocks without footrot. Alternatively, aprB2 persists in flocks without footrot. Despite extensive searching over 3 years only three flocks of sheep without footrot were identified. D. nodosus was not detected in these three flocks. In one further flock, only mild interdigital dermatitis was observed, and only aprB2 was detected. Twenty-four flocks with endemic footrot of all severities were sampled on three occasions and all were positive for D. nodosus and the aprV2 variant; aprB2 was detected in only 11 of these flocks. AprB2 was detected as a co-infection with aprV2 in the 22% of samples positive for aprB2 and was more likely in mild footrot phenotypes than severe. Dichelobacter nodosus serogroups were not associated with footrot phenotype. We conclude that D. nodosus, even aprB2 strains, do not persist in flocks in the absence of footrot. Our results support the hypothesis that aprB2 is associated with mild footrot phenotypes. Finally, we conclude that given the small number of flocks without footrot that were identified, footrot is highly endemic in English sheep flocks.

Evolutionary Study of COVID-19 Disease Severity in India for the Genetic Variation and Prevention of Disease Progression

Introduction:Coronaviruses (CoV) have been responsible for three epidemics in the 21st century, including Severe Acute Respiratory Syndrome (SARS) in 2003. Corona virus is a single strand ribonucleic acid which is genotypically and phenotypically diverse.Method:The phylogenetic tree and Entropy plot are used to show the strain variation with root difference or Boot strip changes among various nucleotide plots with mutational changes (19).Therefore, we would like to explain evolutionary changes in other countries compared to India and in different states of India. To evaluate the strain variation with their mutation and adaptation of the environment with the virus or may be due to several passages occurring over a period of time.Results:For phylogenetic tree analysis, we have selected 8-8 isolates from each state, Assam, Chhattisgarh, Delhi, Gujarat, Haryana, Himachal Pradesh, Karnataka, Kerala, Orissa, Maharashtra, Punjab, Telangana, Uttar Pradesh and West Bengal. Total 74 isolates were selected from given states for making of phylogenetic tree and mutational study. There were many point mutations found.Discussion:According to our study, we found a several point mutation occurred among the sequence submitted in gene bank database that is same as China which has been reported. This study was conducted to systematic analysis on the genomic data of NCBI. This work is also significant in terms of mutational research. Evolutionary changes and approach a new strain through the mutational study done by BioEdit and MEGA 6.0 software. The bioinformatics and the statistical genetic analysis may contribute for study of COVID-19 as other infection and complex disease. This study is used understand the host–pathogen interaction and to contain the COVID-19 outbreak.Conclusion:This study highlighted the evolutionary changes occurred in the COVID-19 disease severity variation in strain for the prevention and mutation. We reported the genetic study of COVID-19 disease with their association in already submitted strain of database for the study of their genetic variation as well as disease progression.