Structure of the holstein dairy cattle subpopulation in Russia by locus CSN2 and CSN3

Purpose: to determine the potential of the Russian subpopulation of the Holstein breed in relation to selection by loci of beta-and kappa-casein.Materials and methods. For loci CSN2 and CSN3, 1,539 Holstein cattle were genotyped, including 1,242 cows and heifers and 297 sires, and information on CSN2 and CSN3 genotypes of 297 US bulls was analyzed (World Wide Sires, Ltd).Results. It has been established that in the last two years there has been an increase in the percentage of sires in WWS with CSN2 genotypes A2A2 and CSN3 genotypes BB. Thus, sires allele frequency in the 2019 catalog was 0.51 and 0.29, respectively; and in the 2021 catalog was 0.68 and 0.31, respectively.Genotyped sires of domestic breeding organizations, which are mostly foreign origin, were characterized by predominance of the A2 allele CSN2; in this group the frequency of the allele was 0.63. However, the CSN3 B allele in the group of sires belonging to domestic organizations was found with a frequency of 0.34, which is 1.6 times lower than that of sires of American origin.In the genotyped group of Holstein cows and heifers (n = 1242) belonging to 3 large farms in the Krasnodar Krai, the gene frequency CSN2 allele A2 was minimal (0.57), and the CSN3 allele B (0.40) was higher than the allele frequency in the sires group of domestic breeding enterprises.Conclusion. At present, Russian breeding enterprises and farms are lagging behind in these indicators, however, by conducting targeted selection in accordance with the indicated trend, by selecting the appropriate breeding bulls, it is possible in the next generation to increase the proportion of animals carrying valuable genotypes.

First results of the archaeoparasitological study of the Volna 1 burial ground (Temryuk District, Krasnodar Krai)

The paper presents the results of an archaeoparasitological analysis of the soil samples from a number of the burials dated to the end of the 4th — first half of the 3rd c. B.C. of the ancient burial ground of Volna 1 situated in the territory of the “Asiatic part” of the Bosporan Kingdom (present-day Temryuk District, Krasnodar Krai). As the result of the investigation, the parasitogenic spectrum of the studied population group has been determined. The eggs of three types of helminths were found. The presence of the eggs of human whipworm (Trichuris trichi-ura) in the soil samples from the burials suggest relatively poor sanitary and hygienic condition of the population and undeveloped hygienic habits of the studied population group. Analysis of the archaeoparasitological data in the historical context and utilization of the archaeopathological material from the archaeological sites of the chronologically close period from the territory of Europe and Asia Minor permitted identification of the range of possible causes of such a situation. The undeveloped hygienic habits are also manifested by finding of of lancet fluke (Dicrocoelium dendriticum) eggs in the soil samples. Besides, the presence in the soil samples of the eggs of this parasite is indicative, from the point of view of archaeoparasitology, of the type of the economy, its methods and, partly, of the dietary specifics. Interesting is the fact of finding of the broad tapeworm (Diphyllobothrium latum) eggs in one of the samples. Given the life cycle of the broad tapeworm and mode of infestation, it is possible to determine unambiguously that the tapeworm infestation might have happened during the consumption of raw underboiled/underroast or dried fish from the freshwater lakes and rivers from the territory of the south of Russia (River Kuban, River Don etc.). Having considered the obtained data in the historical-archaeological con-text, it is possible to hypothesize on the directions of travels of the infested individual. Characteristics of the archaeoparasitological spectrum indicate possible use of anthelminthic agents, or consumption of food with vermicidal effect with respect to the round worms. An important result of the study is demonstration of capabilities of the archaeoparasitology as a source of bioarchaeological information on the population of Northern Black Sea coast.

Key Identity Features of Urban Youth: The Case of Krasnodar Krai

This article is devoted to the study of urban youth local identity in a large and medium city. This identity is manifested in everyday interaction with the urban community, its socio-political institutions and visitors and affect the level of public and political participation, the presence of constructive civic practices. The aim is to identify and describe the main components and place of local youth identity in the system of social identities in large and medium-sized cities of Krasnodar krai (Krasnodar, Novorossiysk, Sochi and Armavir). The empirical basis of the study was made up of focus group transcripts conducted with various groups of young people (schoolchildren, students, and working youth). To verify the conceptual model a modified version of the Kuhn-McPartland method was used. On the basis of the conducted empirical research, the place of local identity in the system of urban youth social and territorial identities was revealed. The dependence between the size of a city and a cohort of young people and a local identity was shown. Such components of young people local identity as awareness of the city and its socio-political life, attitude towards representatives of other communities, a sense of their involvement in city life, the desire to stay and live in the city, the will to work for the benefit of the city, to participate in its socio-political life. The study made it possible to identify the valence of youth identity (negative, neutral, positive). The trajectories of young people spatial mobility that affect the degree of actualization and valence of local identity were also described. The dependence between the strength of youth local identity and participation in public and political activity for the benefit of the city and the region, participation in the activities of public and political organizations has been revealed.

Youth Public Associations’ Political Engagement in Russia: The Case of Krasnodar Krai

The article defines the place and role of youth public associations in the socio-political processes of modern Russia, analyzes the political activity of Russian youth. The study was conducted among young people in the Krasnodar region using the questionnaire developed by the author, as well as methods for determining the level of activity and motivation of the population. The data obtained allow us to determine the degree of involvement of young people in the activities of NGOs and political processes in the Krasnodar territory. The conducted research has shown the strengthening of the influence of youth public associations on socio-political processes, personnel policy, activation of their activities in the field of public diplomacy, volunteer movement, articulation of the interests of young people as a special social group of the population. Youth public associations are able to structure and regulate the activity of young Russian citizens, directing it in a constructive direction, while reducing the degree of protest moods if it is present. They do this work more effectively than the authorized state authorities, because they act on the ground, i.e. they work directly with representatives of the youth community, understand their needs, catch the mood and are able to change the current agenda without resorting to radical methods.

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

The use of high-throughput sequencing (HTS) technology has led to significant progress in the identification of many viruses and their genetic variants. In this study, we used the HTS platform to sequence small RNAs (sRNAs) of grapevine to study the virome. Isolation of RNA was performed using symptomatic grapevines collected from commercial vineyards in Krasnodar Krai in 2017–2018. To determine the viromes of vineyards, we used an integrated approach that included a bioinformatic analysis of the results of sRNA HTS and the molecular method RT-PCR, which made it possible to identify 13 viruses and 4 viroids. Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine Syrah Virus-1 (GSyV-1), Raspberry bushy dwarf virus (RBDV), Australian grapevine viroid (AGVd), and Grapevine yellow speckle viroid 2 (GYSVd-2) were identified for the first time in Russia. Out of 38 samples analyzed, 37 had mixed infections with 4–11 viruses, indicating a high viral load. Analysis of the obtained sequences of fragments of virus genomes made it possible to identify recombination events in GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4, GVT, GPGV, GRSPaV, GVA, and GFLV. The obtained results indicate a wide spread of the viruses and a high genetic diversity in the vineyards of Krasnodar Krai and emphasize the urgent need to develop and implement long-term strategies for the control of viral grapevine diseases.

Integrity of risk indicators of hazardous natural processes (on the example of the Krasnodar Krai)

The problem of assessing risk indicators of hazardous natural processes as the basis for sustainable development of the region arises due to the peculiarities of the geological structure of the territory of Krasnodar Krai. The work is based on a comprehensive assessment of the geological conditions of the territory of Krasnodar Krai with the use of a systematic approach. A list of characteristic natural processes that pose the greatest danger to capital constructions was determined: in the mountainous part – erosion processes, on the plain – soil subsidence and associated landforms. Then, schematic maps were compiled and the geological component of the risk (its spatial-areal indicator) was determined. Subsequently, to compare these maps, overlay operations were performed, as a result of which the spatial characteristics of ARC/INFO layers were combined into a new layer and relational join of their attribute data was carried out. Adding the risks of each parameter made it possible to rank the territory into 4 zones according to the degree of integral risk of manifestation of natural processes.

Energy potential of modern wind turbines in the Krasnodar Krai

Krasnodar Krai is an intensively economically developing region. In terms of the activity in the construction of residential and industrial facilities, it ranks among the leaders in Russia. At the same time, the region is experiencing a serious shortage of energy produced on its territory. The power system of the Krasnodar Krai is characterized by a high specific consumption of electric energy per capita, comparable in size to the largest cities of the Russian Federation. Also, the territory of the Krasnodar Krai is a recreational area, a valuable ecological object, where the construction of carbon-burning power plants is undesirable due to the high degree of environmental pollution. The construction of a nuclear power plant is also unacceptable due to the seismic activity of the territory. Based on the above, it is necessary to analyze the possible use of alternative energy sources, in particular, wind energy, since the wind potential of the territory of the region is large. In the paper, the wind potential of the coast of the Krasnodar Krai was studied - water space, estuaries and reservoirs that are not used or are poorly used for recreational and economic purposes, which have indicators of wind speed and constancy more than on land, and which are not considered by most researchers due to unusual location.

Alleles of the GRF3-2A Gene in Wheat and Their Agronomic Value

The Growth-regulating factors (GRF) are a family of plant-specific transcription factors that have roles in plant growth, development and stress response. In this study the diversity of the TaGRF3-2A (TraesCS2A02G435100) gene was investigated in Russian bread wheat germplasm by means of next generation sequencing and molecular markers, and the results compared with those from multiple wheat genome and exome sequencing projects. The results showed that an allele possessing c.495G>T polymorphism found in Bezostaya 1 and designated as TaGRF3-2Ab, is connected with earlier heading and better grain filling under conditions of the Krasnodar Krai. TaGRF3-2Ab is more frequent among Russian winter wheat cultivars than in other germplasms found in the world, implying that it is adaptive for the Chernozem region. A new rare mutation of the TaGRF3-2A was found in the spring wheat cultivar Novosibirskaya 67. The molecular markers developed will facilitate utilization of TaGRF3-2A mutations in future agronomic studies and wheat improvement. Albeit GRF3-2Ab may be good at maintaining high milling quality of the grain, it should be used with caution in breeding of winter wheat cultivars in the perspective of climate change.