Abstract
Background Gentamicin is a crucial aminoglycoside antibiotic but it is used only to treat severe bacterial infections, because of its high nephrotoxicity among patients. We evaluated the preventive effects of diosmin (as a natural ingredient) on gentamicin-related renal damage in rats. MethodsIn this research, 28 male Wistar rats were assigned to 4 groups: control, gentamicin (100 mg/kg, (i.p.), daily for 1 week), and gentamicin plus diosmin (50mg/kg, p.o., daily for two weeks), diosmin (50mg/kg/day, p.o. for two weeks). After, the final gavage, blood specimens were gathered for determining serum blood urea nitrogen (BUN) and creatinine. kidneys used for biochemical, inflammation and histological test.Results Creatinine, BUN, nitric oxide, malondialdehyde, TNF-α and IL-1β concentrations significantly increased and glutathione, catalase, glutathione peroxidase, and superoxide dismutase activities decreased after gentamicin treatment. Creatinine, BUN, nitric oxide, malondialdehyde, tumour necrosis factor α (TNF-α), interleukin 1 beta (IL-1β) concentrations significantly reduced and glutathione level, catalase and glutathione peroxidase activities significantly increased via co-administration with diosmin. ConclusionDiosmin had ameliorative impacts against gentamicin-related kidney injury that can be owing to its antioxidant, and anti-inflammatory activities.