Stability of Transgene Inheritance in Progeny of Field-Grown Pear Trees over a 7-Year Period

Breeding woody plants is a very time-consuming process, and genetic engineering tools have been used to shorten the juvenile phase. In addition, transgenic trees for commercial cultivation can also be used in classical breeding, but the segregation of transgenes in the progeny of perennial plants, as well as the possible appearance of unintended changes, have been poorly investigated. We studied the inheritance of the uidA gene in the progeny of field-grown transgenic pear trees for 7 years and the physical and physiological parameters of transgenic seeds. A total of 13 transgenic lines were analyzed, and the uidA gene segregated 1:1 in the progeny of 9 lines and 3:1 in the progeny of 4 lines, which is consistent with Mendelian inheritance for one and two transgene loci, respectively. Rare and random deviations from the Mendelian ratio were observed only for lines with one locus. Transgenic seeds’ mass, size, and shape varied slightly, despite significant fluctuations in weather conditions during cultivation. Expression of the uidA gene in the progeny was stable. Our study showed that the transgene inheritance in the progeny of pear trees under field conditions occurs according to Mendelian ratio, does not depend on the environment, and the seed vigor of transgenic seeds does not change.

Application of gas analyzers for the control of fuel in the air environment of rocket and space industry enterprises

The presented work shows the necessity of organization of individual instrumental control of the content of rocket fuel and its products in the air of rocket-space industry enterprises. No accurate analysis of gas analyzers presented on the Russian market, providing individual instrumental control of concentrations of heptyl and its derivatives in the workplace air is available. Therefore, the task of express, automatic, selective and simultaneous monitoring of heptyl vapors and derivatives of its transformation in the air of industrial premises by one instrument and replacing the imported gas analyzers remains urgent. It is shown that the solution of the posed problem is possible on the basis of the optical absorption method. The requirements to be met by the gas analyzer are formulated. Approximate wavelengths at which simultaneous measurement of concentrations of heptyl and its derivatives in an air sample is possible have been determined. The ways of realization of individual gas analyzers having small mass-size characteristics are outlined. Structural schemes for infra-red gas analyzers have been proposed: multi-channel; single-channel (with frequency modulation of radiation). Tasks for further research are formulated.

The Robustness of Synthetic Observations in Producing Observed Core Properties: Predictions for the TolTEC Clouds to Cores Legacy Survey

We use hydrodynamical simulations of star-forming gas with stellar feedback and sink particles—proxies for young stellar objects (YSOs)—to produce and analyze synthetic 1.1 mm continuum observations at different distances (150–1000 pc) and ages (0.49–1.27 Myr). We characterize how the inferred core properties, including mass, size, and clustering with respect to diffuse natal gas structure, change with distance, cloud evolution, and the presence of YSOs. We find that atmospheric filtering and core segmentation treatments have distance-dependent impacts on the resulting core properties for d < 300 pc and 500 pc, respectively, which dominate over evolutionary differences. Concentrating on synthetic observations at further distances (650–1000 pc), we find a growing separation between the inferred sizes and masses of cores with and without YSOs in the simulations, which is not seen in recent observations of the Monoceros R2 (Mon R2) cloud at 860 pc. We find that the synthetic cores cluster in smaller groups, and that their mass densities are correlated with gas column density over a much narrower range, than those in the Mon R2 observations. Such differences limit the applicability of the evolutionary predictions we report here, but will motivate our future efforts to adapt our synthetic observation and analysis framework to next generation simulations, such as Star Formation in Gaseous Environments (STARFORGE). These predictions and systematic characterizations will help to guide the analysis of cores on the upcoming TolTEC Clouds to Cores Legacy Survey on the Large Millimeter Telescope Alfonso Serrano.

The chemotherapy effect on embryonal rhabdomyosarcoma in a 2-year-old girl in a development country: A rare case

Rhabdomyosarcoma (RMS) is the third most common solid organ malignancy in children after Wilms tumor and neuroblastoma. It is a highly malignant tumor with local tissue invasion and lymphatic and hematogenous metastases. The main treatment for RMS is complete resection with a combination of chemotherapy and radiotherapy. Here, we present the case of a 2-year-old girl who came to the hospital with a complaint of noisy breathing (Stridor) for the past 2 weeks. Intraoral examination showed an oval mass at the base of the tongue that almost closed the airway, felt soft, and had a bump. A computed tomography scan of the neck was performed, and a solid lesion with clear irregular borders was found in the posterior lingua. Incisional biopsy was performed with histopathological results of embryonal RMS. Two weeks after the biopsy, chemotherapy was performed, and the evaluation after two chemotherapy sessions, the mass size was found to have decreased.

Retrievals of ice microphysics using dual-wavelength polarimetric radar observations during stratiform precipitation events

Ice growth processes within clouds affect the type as well as the amount of precipitation. Hence, the importance of an accurate representation of ice microphysics in numerical weather and numerical climate models has been confirmed by several studies. To better constrain ice processes in models, we need to study ice cloud regions before and during monitored precipitation events. For this purpose, two radar instruments facing each other were used to collect complementary measurements. The C-band POLDIRAD weather radar from the German Aerospace Center (DLR), Oberpfaffenhofen and the Ka-band MIRA-35 cloud radar from the Ludwig Maximilians University of Munich (LMU) were used to monitor stratiform precipitation in the vertical cross-section area between both instruments. The logarithmic difference of radar reflectivities at two different wavelengths (54.5 and 8.5 mm), known as dual-wavelength ratio, was exploited to provide information about the size of the detected ice hydrometeors, taking advantage of the different scattering behavior in the Rayleigh and Mie regime. Along with the dual-wavelength ratio, differential radar reflectivity measurements from POLDIRAD provided information about the apparent shape of the detected ice hydrometeors. Scattering simulations using the T-matrix method were performed for oblate and horizontally aligned prolate ice spheroids of varying shape and size using a realistic particle size distribution and a well-established mass-size relationship. The combination of dual-wavelength ratio, radar reflectivity and differential radar reflectivity measurements as well as scattering simulations was used for the development of a novel retrieval for ice cloud microphysics. The development of the retrieval scheme also comprised a method to estimate the hydrometeor attenuation in both radar bands. To demonstrate this approach, a feasibility study was conducted on three stratiform snow events which were monitored over Munich in January 2019. The ice retrieval can obtain ice particle shape, size and mass which are in line with differential radar reflectivity, dual-wavelength ratio and radar reflectivity observations when a suitable mass-size relation is used and when ice hydrometeors are assumed to be represented by oblate ice spheroids. A furthermore finding was the importance of the differential radar reflectivity for the particle size retrieval directly above the MIRA-35 cloud radar. Especially for that observation geometry, the simultaneous slantwise observation from the polarimetric weather radar POLDIRAD could reduce ambiguities in retrieval of the ice particle size by constraining the ice particle shape.

Temperature Environmental Adaptability Research of HNIW/FOX-7 Based PBXs

In this study, HNIW/FOX-7 based PBX modeling powders and PBX columns were treated by LT (low temperature), HT (high temperature), HLC (high-low temperature cycle) and HLS (high-low temperature shock) to study temperature environmental adaptability of HNIW/FOX-7 based PBXs. Then SEM, IR, XRD and DSC were used to study the variation of PBX modeling powders after LT, HT, HLC and HLS treatments; in addition, the mass, size and mechanical properties of PBX columns were characterized after different temperature adaptability treatments as well. The results indicate that the change ratios of mass and size of HNIW/FOX-7 based PBX columns are less than 1%, illustrating that mass and size of PBX columns are at acceptable level after different temperature adaptability treatments. The unevenness degree of the surface of PBX modeling powders followed the order of HLC>HT>LT>HLS, which agrees well with mass loss order. Moreover, IR and XRD results indicated that the molecular structure and crystal form of HNIW and FOX-7 did not change after different temperature adaptability treatments. Additionally, thermal stabilities of PBX modeling powders are decreased after different temperature adaptability treatments, among which HLS has the largest influence on HNIW/FOX-based PBX modeling powders. The compression strengths and elastic moduli of HNIW/FOX-based PBX columns are enhanced after different temperature adaptability treatments, among which the strength of PBX columns after HLC has the maximum increase, indicating that HLC has more significant effect on mechanical property.

The relevance of rock shape over mass—implications for rockfall hazard assessments

The mitigation of rapid mass movements involves a subtle interplay between field surveys, numerical modelling, and experience. Hazard engineers rely on a combination of best practices and, if available, historical facts as a vital prerequisite in establishing reproducible and accurate hazard zoning. Full-scale field tests have been performed to reinforce the physical understanding of debris flows and snow avalanches. Rockfall dynamics are - especially the quantification of energy dissipation during the complex rock-ground interaction - largely unknown. The awareness of rock shape dependence is growing, but presently, there exists little experimental basis on how rockfall hazard scales with rock mass, size, and shape. Here, we present a unique data set of induced single-block rockfall events comprising data from equant and wheel-shaped blocks with masses up to 2670 kg, quantifying the influence of rock shape and mass on lateral spreading and longitudinal runout and hence challenging common practices in rockfall hazard assessment.

Cross-pollination affects fruit colour, acidity, firmness and shelf life of self-compatible strawberry

Cross-pollination affects the fruit characteristics of many crops but the effects of cross-pollination on fruit quality of strawberry (Fragaria × ananassa Duch.) are poorly known. This study determined how cross-pollination affects fruit quality of the strawberry cultivar, Redlands Joy, under controlled environment conditions. Plants were allocated to one of four treatments, with all flowers on each plant receiving either: (1) unassisted self-pollination (Autogamy); (2) hand-pollination with Redlands Joy pollen (Self); (3) hand-pollination with cross-pollen from a small-fruited cultivar (Sugarbaby); or (4) hand-pollination with cross-pollen from a large-fruited cultivar (Rubygem). Cross-pollination did not significantly affect plant yield or fruit mass, size, shape, firmness or shelf life. However, cross-pollination affected fruit colour and taste attributes. Cross-pollinated fruit were 3%–5% darker than self-pollinated fruit. They also had 26%–34% lower acidity and 43%–58% higher Brix:acid ratio. Cross-pollination by Sugarbaby increased fruit P, K, Ca, Fe and Mn, but decreased B, Cu and Zn, concentrations. Cross-pollination by Rubygem increased fruit Mn, but decreased K and Na, concentrations and reduced shelf life. Fruit mass, length, diameter and firmness within all treatments increased with increasing numbers of fertilized seeds per fruit. Hand self-pollinated fruit had a higher percentage of fertilized seeds than fruit arising from autogamy and they were also darker, redder, firmer, and had a longer shelf life, higher protein concentration, and lower Al and Na concentrations. The results indicate that strawberry fruit quality can be affected by both the source of pollen and the number of stigmas pollinated.

Colonic actinomycosis presenting as a palpable colonic mass with normal colonic mucosa

Colonic actinomycosis is rare and can present as an ill-defined intra-abdominal mass that can be difficult to differentiate from colon cancer. This case report aims to share the details of this case and provide diagnostic clues. A 63-year-old female presented with a palpable right-sided abdominal mass. Computed tomography (CT) revealed irregular thickening of the colonic hepatic flexure, and colonoscopy detected no abnormalities. Five months later, the patient returned with an increase in the mass size. Repeat CT revealed lesion expansion, with suspected abdominal wall invasion. Extended right-hemicolectomy with abdominal wall wedge resection was performed, and the histological results were compatible with actinomycosis infection. Colonic actinomycosis is a rare chronic inflammatory disease. Normal colonic mucosa during colonoscopy, with clinical and imaging findings, may help physicians diagnose the condition preoperatively.