This study aims at identifying the challenges facing undergraduates in the use of electronic information resources (EIR) in a university in Southwest Nigeria. The descriptive survey design was adopted for this study. The population for the study comprised 85,526 registered undergraduate student (2018/2019) academic session in the federal and state universities in Southwest Nigeria. The sample for the study was 398. Proportionate sampling techniques were used to draw the sample. Questionnaire was the instrument used for data collection from the respondents based on a four-point scale rating. The findings revealed high cost of access, power outage, information overload, lack of proper maintenance, funding of library services, among others. Based on the findings, the study recommended provision of alternative sources of funds, constant maintenance of e-resources, and first year students should be introduced to computer training, among others.