Oral Administration of Bacillus subtilis Subunit Vaccine Significantly Enhances the Immune Protection of Grass Carp against GCRV-II Infection

Grass carp reovirus (GCRV) is a severe virus that causes great losses to grass carp culture every year, and GCRV-II is the current popular and fatal strain. VP56, fibrin on the outer surface of GCRV-II, mediates cell attachment. In this study, we firstly divided the VP56 gene into four fragments to screen the optimal antigen by enzyme-linked immunosorbent assay and neutralizing antibody methods. The second fragment VP56-2 demonstrates the optimal efficiency and was employed as an antigen in the following experiments. Bacillus subtilis were used as a carrier, and VP56-2 was expressed on the surface of the spores. Then, we performed the oral immunization for grass carp and the challenge with GCRV-II. The survival rate was remarkably raised, and mRNA expressions of IgM were significantly up-regulated in spleen and head kidney tissues in the B. s-CotC-VP56-2 group. Three crucial immune indexes (complement C3, lysozyme and total superoxide dismutase) in the sera were also significantly enhanced. mRNA expressions of four important genes (TNF-α, IL-1β, IFN1 and MHC-II) were significantly strengthened. Tissue lesions were obviously attenuated by histopathological slide examination in trunk kidney and spleen tissues. Tissue viral burdens were significantly reduced post-viral challenge. These results indicated that the oral recombinant B. subtilis VP56-2 subunit vaccine is effective for controlling GCRV infection and provides a feasible strategy for the control of fish virus diseases.

Collaborative Optimization Method of Power and Efficiency for LCC-S Wireless Power Transmission System

Dynamic wireless charging enables moving equipment such as electric vehicles, robots to be charged in motion, and thus is a research hotspot. The applications in practice, however, suffer from mutual inductance fluctuation due to unavoidable environmental disturbances. In addition, the load also changes during operation, which makes the problem more complicated. This paper analyzes the impacts of equivalent load and mutual inductances variation over the system by LCC-S topology modeling utilizing two-port theory. The optimal load expression is derived. Moreover, a double-sided control strategy enabling optimal efficiency and power adjustment is proposed. Voltage conducting angles on the inverter and rectifier are introduced. The simulation and experimental results verify the proposed method.

Optimal Design Methodology on Compensation Parameters of Inductive Power Transfer Converter for Electric Vehicles

Compensation topologies of the inductive power transfer (IPT) converter for electric vehicles (EVs) have been researched in previous works. However, a methodology for designing a compensation topology based on the efficiency of the IPT converter has been barely discussed. This paper proposes an optimal design methodology for compensation parameters to achieve optimal efficiency of the IPT converter with LCC-S. The optimal output voltage is derived using the losses analysis of the IPT converter, and the IPT converter is designed for the optimal output voltage to achieve the optimal efficiency. Furthermore, the battery management (BM) converter on the receiving side is designed based on the output voltage of the IPT converter, the fluctuation range of the coupling coefficient, and the battery charging voltage. The validity of the proposed IPT converter design methodology is verified by designing different compensation parameters and BM converters. The power rating of the three design cases is 3.3 kW with the same magnetic pads satisfying the SAE J2954 WPT 1 class.

No part gets left behind: Tiled nanopore sequencing of whole ASFV genomes stitched together using Lilo

African Swine Fever virus (ASFV) is the causative agent of a deadly, panzootic disease, infecting wild and domesticated suid populations. Contained for a long time to the African continent, an outbreak of a particularly infectious variant in Georgia in 2007 initiated the spread of the virus around the globe, severely impacting pork production and local economies. The virus is highly contagious and has a mortality of up to 100% in domestic pigs. It is critical to track the spread of the virus, detect variants associated with pathology, and implement biosecurity measures in the most effective way to limit its spread. Due to its size and other limitations, the 170-190kbp large DNA virus has not been well sequenced with fewer than 200 genome sequences available in public repositories. Here we present an efficient, low-cost method of sequencing ASFV at scale. The method uses tiled PCR amplification of the virus to achieve greater coverage, multiplexability and accuracy on a portable sequencer than achievable using shotgun sequencing. We also present Lilo, a pipeline for assembling tiled amplicon data from viral or microbial genomes without relying on polishing against a reference, allowing for structural variation and hypervariable region assembly other methods fail on. The resulting ASFV genomes are near complete, lacking only parts of the highly repetitive 3’- and 5’telomeric regions, and have a high level of accuracy. Our results will allow sequencing of ASFV at optimal efficiency and high throughput to monitor and act on the spread of the virus.

Comparing Light—Emitting—Diodes Light Traps for Catching Anopheles Mosquitoes in a Forest Setting, Western Thailand

Light traps are a common method for attracting and collecting arthropods, including disease vectors such as mosquitoes. Various types of traps have been used to monitor mosquitoes in a forest in Western Thailand. In this study, four Light Emitting Diodes (LED) light sources (UV, blue, green, and red) and two fluorescent lights (white and UV) were used to trap nocturnal adult mosquitoes. These traps were used with light alone and not any additional attractant. The experiment was conducted from 18:00 to 06:00 h. on six consecutive nights, every two months, across dry, wet, and cold seasons. All specimens were first identified by morphological features and subsequently confirmed by using PCR. We collected a total of 873 specimens of 31 species in four genera, Anopheles, Aedes, Culex, and Armigeres. Anopheles harrisoni was the predominant species, followed by Aedes albopictus, Culex brevipalpis, Culex nitropunctatus, and Armigeres (Leicesteria) longipalpis. UV fluorescent light was the most effective light source for capturing forest mosquitoes, followed by UV LED, blue LED, green LED, white fluorescent, and red LED. The optimal times for collection were from 21:00 to 03:00 h in the dry season. Our results demonstrate that appropriate sampling times and light sources should be selected for optimal efficiency in vector surveillance programs.

Severe Accident Management Systems and Procedures

A nuclear power plant’s safe operation involves the planning for non-standard operational emergencies, where pre-determined safety measures and damage control interventions must be taken into consideration depending on the developed event. A nuclear power plant’s safe operation involves the planning for non-standard operational emergencies, where pre-determined safety measures and damage control interventions must be taken into consideration depending on the developed event. The definition of the severe accident management cannot be explained in a single concept, it needs to be examined in detail. As a result of this it becomes necessary to specify the procedure guidelines appropriate to the nature of the event, which can be used with optimal efficiency under hierarchical organisational control. The experience of nuclear accidents in the world and the precognition of future events, the knowledge of existing guidelines for severe accident management needs to be deepened continuously, but at the same time it can be parallelly upgraded with the application of new technologies.

The performance of a flapping foil for a self-propelled fishlike body

Several fish species propel by oscillating the tail, while the remaining part of the body essentially contributes to the overall drag. Since in this case thrust and drag are in a way separable, most attention was focused on the study of propulsive efficiency for flapping foils under a prescribed stream. We claim here that the swimming performance should be evaluated, as for undulating fish whose drag and thrust are severely entangled, by turning to self-propelled locomotion to find the proper speed and the cost of transport for a given fishlike body. As a major finding, the minimum value of this quantity corresponds to a locomotion speed in a range markedly different from the one associated with the optimal efficiency of the propulsor. A large value of the feathering parameter characterizes the minimum cost of transport while the optimal efficiency is related to a large effective angle of attack. We adopt here a simple two-dimensional model for both inviscid and viscous flows to proof the above statements in the case of self-propelled axial swimming. We believe that such an easy approach gives a way for a direct extension to fully free swimming and to real-life configurations.

Integration of Microfluidics, Photonic Integrated Circuits and Data Acquisition and Analysis Methods in a Single Platform for the Detection of Swine Viral Diseases

Viral diseases challenge the health and welfare of pigs and undermine the sustainability of swine farms. Their efficient control requires early and reliable diagnosis, highlighting the importance of Point of Care (POC) diagnostics in veterinary practice. The objective of this study was to validate a novel POC system that utilizes Photonic Integrated Circuits (PICs) and microfluidics to detect swine viral pathogens using oral fluids and Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV-2) as proofs of concept. The sensitivity and specificity of the device were calculated for both viruses, and Receiver Operating Characteristic (ROC) curves were drawn. PPV had an Area Under Curve (AUC) value of 0.820 (95% CI: 0.760 to 0.880, p < 0.0001), and its optimal efficiency threshold of detection shifts was equal to 4.5 pm (68.6% sensitivity, 77.1% specificity and Limit of Detection (LOD) value 106 viral copies/mL). PCV-2 had an AUC value of 0.742 (95% CI: 0.670 to 0.815, p < 0.0001) and an optimal efficiency threshold of shifts equal to 6.5 pm (69.5% sensitivity, 70.3% specificity and LOD 3.3 × 105 copies/mL). In this work, it was proven that PICs can be exploited for the detection of swine viral diseases. The novel device can be directly deployed on farms as a POC diagnostics tool.

Dynamic Resource Allocation in the Cloud with Near-Optimal Efficiency

Motivated by maximizing spot instances in cloud shared systems, in this work, we consider the problem of taking advantage of unused resources in highly dynamic cloud environments while preserving users’ performance. We introduce an online model for sharing resources that captures basic properties of cloud systems, such as unpredictable users’ demand patterns, very limited feedback from the system, and service level agreement (SLA) between the users and the cloud provider. We provide a simple and efficient algorithm for the single-resource case. For any demand patterns, our algorithm guarantees near-optimal resource utilization as well as high users’ performance compared with their SLA baseline. In addition to this, we validate empirically the performance of our algorithm using synthetic data and data obtained from Microsoft’s systems.