scholarly journals Apolipoprotein M promotes cholesterol uptake and efflux from mouse macrophages

FEBS Open Bio ◽  
2021 ◽  
Author(s):  
Shuang Yao ◽  
Fan Zheng ◽  
Yang Yu ◽  
Yuxia Zhan ◽  
Ning Xu ◽  
...  
Keyword(s):  
Apolipoprotein M ◽  
Cholesterol Uptake ◽  
Mouse Macrophages

Negative Correlation Between Serum Levels of Homocysteine and Apolipoprotein M

2019 ◽  
Vol 19 (2) ◽  
pp. 120-126
Author(s):  
J. Wei ◽  
Y. Yu ◽  
Y. Feng ◽  
J. Zhang ◽  
Q. Jiang ◽  
...  
Keyword(s):  
Negative Correlation ◽  
Hepg2 Cells ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Apolipoprotein M ◽  
Protein Mass ◽  
Protein Levels ◽  
Phosphoinositide 3 Kinase

Background: Homocysteine (Hcy) has been suggested as an independent risk factor for atherosclerosis. Apolipoprotein M (apoM) is a constituent of the HDL particles. The goal of this study was to examine the serum levels of homocysteine and apoM and to determine whether homocysteine influences apoM synthesis. Methods: Serum levels of apoM and Hcy in 17 hyperhomocysteinemia (HHcy) patients and 19 controls were measured and their correlations were analyzed. Different concentrations of homocysteine (Hcy) and LY294002, a specific phosphoinositide 3- kinase (PI3K) inhibitor, were used to treat HepG2 cells. The mRNA levels were determined by RT-PCR and the apoM protein mass was measured by western blot. Results: We found that decreased serum apoM levels corresponded with serum HDL levels in HHcy patients, while the serum apoM levels showed a statistically significant negative correlation with the serum Hcy levels. Moreover, apoM mRNA and protein levels were significantly decreased after the administration of Hcy in HepG2 cells, and this effect could be abolished by addition of LY294002. Conclusions: resent study demonstrates that Hcy downregulates the expression of apoM by mechanisms involving the PI3K signal pathway.

scholarly journals Angptl4 Upregulates Cholesterol Synthesis in Liver via Inhibition of LPL- and HL-Dependent Hepatic Cholesterol Uptake

2007 ◽  
Vol 27 (11) ◽  
pp. 2420-2427 ◽  
Author(s):  
Laeticia Lichtenstein ◽  
Jimmy F.P. Berbée ◽  
Susan J. van Dijk ◽  
Ko Willems van Dijk ◽  
André Bensadoun ◽  
...  
Keyword(s):  
Cholesterol Synthesis ◽  
Hepatic Cholesterol ◽  
Cholesterol Uptake

Immunoprecipitation Strategies to Isolate RIPK1/RIPK3 Complexes in Mouse Macrophages

2021 ◽  
Vol 1 (6) ◽  
Author(s):  
Ioannis Siokas ◽  
Dingqiang Zhang ◽  
Alexander Poltorak ◽  
Hayley Muendlein ◽  
Alexei Degterev
Keyword(s):  
Mouse Macrophages
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