Towards High-Throughput Cell Mechanics Assays for Research and Clinical Applications

2012 ◽  
pp. 255-292
Author(s):  
David R. Myers ◽  
Daniel A. Fletcher ◽  
Wilbur A. Lam
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Clinical Applications

scholarly journals Therapeutic Drugs Targeting 2019-nCoV Main Protease by High-Throughput Screening

2020 ◽  
Author(s):  
Yan Li ◽  
Jinyong Zhang ◽  
Ning Wang ◽  
Haibo Li ◽  
Yun Shi ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Binding Capacity ◽  
Effective Means ◽  
Clinical Applications ◽  
Therapeutic Drugs ◽  
Main Protease ◽  
Novel Coronavirus ◽  
Clinical Drug ◽  
Specific Inhibitors

Abstract2019 Novel Coronavirus (2019-nCoV) is a virus identified as the cause of the outbreak of pneumonia first detected in Wuhan, China. Investigations on the transmissibility, severity, and other features associated with this virus are ongoing. Currently, there is no vaccine or therapeutic antibody to prevent the infection, and more time is required to develop an effective immune strategy against the pathogen. In contrast, specific inhibitors targeting the key protease involved in replication and proliferation of the virus are the most effective means to alleviate the epidemic. The main protease of SARS-CoV is essential for the life cycle of the virus, which showed 96.1% of similarity with the main proteaseof 2019-nCoV, is considered to be an attractive target for drug development. In this study, we have identified 4 small molecular drugs with high binding capacity with SARS-CoV main protease by high-throughput screening based on the 8,000 clinical drug libraries, all these drugs have been widely used in clinical applications with guaranteed safety, which may serve as promising candidates to treat the infection of 2019-nCoV.

scholarly journals Versatile and High-throughput Force Measurement Platform for Dorsal Cell Mechanics

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Seungman Park ◽  
Yoon Ki Joo ◽  
Yun Chen
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Force Measurement ◽  
Time Lapse ◽  
Dorsal Side ◽  
Single Particle Tracking ◽  
Single Experiment ◽  
Molecular Binding ◽  
Force Magnitude

Abstract We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted on the cell can be modulated ranging from ~14 pN to 2 nN to perturb various force-dependent processees in cells. Time-lapse images were acquired to record events due to such perturbation. The values of various mechanical parameters are subsequently obtained by single particle tracking. Up to 50 events can be measured simultaneously in a single experiment. Integrating the microfluidic techniques with the analytic framework established in computational fluid dynamics, our method is physiologically relevant, reliable, economic and efficient.

Computational Approaches Leveraging Integrated Connections of Multi-Omic Data toward Clinical Applications

2021 ◽  
Author(s):  
Cansu H Demirel ◽  
Kaan M Arici ◽  
Nurcan Tuncbag
Keyword(s):  
High Throughput ◽  
Biological Systems ◽  
Clinical Applications ◽  
Data Type ◽  
Omics Data ◽  
Computational Approaches ◽  
Omic Data

In line with the advances in high-throughput technologies, multiple omic datasets have accumulated to study biological systems and diseases coherently. No single omics data type is capable of fully representing...

High-throughput measurements of cell mechanics using atomic force microscopy with micro-patterned substrates

MHS2013 ◽  
2013 ◽  
Author(s):  
Ryosuke Takahashi ◽  
Kaori Kuribayashi-Shigetomi ◽  
Masahiro Tsuchiya ◽  
Agus Subagyo ◽  
Kazuhisa Sueoka ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Throughput ◽  
Cell Mechanics ◽  
Patterned Substrates ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals High-throughput ballistic injection nanorheology to measure cell mechanics

2012 ◽  
Vol 7 (1) ◽  
pp. 155-170 ◽  
Author(s):  
Pei-Hsun Wu ◽  
Christopher M Hale ◽  
Wei-Chiang Chen ◽  
Jerry S H Lee ◽  
Yiider Tseng ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Mechanics

scholarly journals An Apodictic Review on Recent Approaches in Enzyme Technology

2021 ◽  
Vol 12 (3) ◽  
pp. 3446-3471
Keyword(s):  
Enzyme Activity ◽  
High Throughput ◽  
Industrial Applications ◽  
Clinical Applications ◽  
Living Systems ◽  
Activity Prediction ◽  
Research Teams ◽  
Production Technologies ◽  
Historical Periods ◽  
Enzyme Source

Enzymes are the most powerful biochemical moieties, predominantly the working tools in all living systems. Many studies have revealed the usage of various enzymes even in the pre-historical periods. Enzymes are known to be the extremely active biocatalyst that is widely involved in many metabolisms. Living systems explore these biomolecules for their metabolism and are exhaustively explored for various industrial and clinical applications. Due to the increasing need for enzyme-based products, various recent research focuses on exploring distinct enzymes & enzyme sources with relatively enhanced characteristics. The elegant motive of this review is to enable the readers and enzyme researchers to compend the basics of enzymes, explore the enormous recent clinical & industrial applications of enzymes like amylase, cellulase, protease, lipase, and esterase. And also, the review highly emphasizes the various enzyme source and their enriched properties like enzyme activity, annotated by recent research works carried out by various research teams across the globe. The review also accentuates the recent advancements in production technologies and high throughput activity prediction assays for the above-mentioned industrially important enzymes.

scholarly journals Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling

2003 ◽  
Vol 2003 (5) ◽  
pp. 299-307 ◽  
Author(s):  
Weiping Shao ◽  
Zhimin Zhou ◽  
Isabelle Laroche ◽  
Hong Lu ◽  
Qiuling Zong ◽  
...  
Keyword(s):  
High Throughput ◽  
Signal Amplification ◽  
Protein Microarray ◽  
Cost Effective ◽  
Protein Microarrays ◽  
Clinical Applications ◽  
Protein Profiling ◽  
Antibody Microarray ◽  
High Throughput Analysis ◽  
Rolling Circle

Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/ml range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics.

Sign in / Sign up

Export Citation Format

Share Document