DNA‐based microparticle tension sensors (µTS) for measuring cell mechanics in non‐planar geometries and for high‐throughput quantification

2021 ◽  
Author(s):  
Yuesong Hu ◽  
Victor Pui-Yan Ma ◽  
Rong Ma ◽  
Wenchun Chen ◽  
Yuxin Duan ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Measuring Cell ◽  
Planar Geometries

scholarly journals High-Throughput GLP-Capable Target Cell Visualization Assay for Measuring Cell-Mediated Cytotoxicity

Cells ◽  
2018 ◽  
Vol 7 (5) ◽  
pp. 35 ◽  
Author(s):  
Anna Welter ◽  
Srividya Sundararaman ◽  
Ruliang Li ◽  
Ting Zhang ◽  
Alexey Karulin ◽  
...  
Keyword(s):  
High Throughput ◽  
Target Cell ◽  
Measuring Cell

scholarly journals Measuring Cell Mechanics by Optical Alignment Deformation Spectroscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 177a
Author(s):  
Kevin B. Roth ◽  
Keith B. Neeves ◽  
David W.M. Marr
Keyword(s):  
Cell Mechanics ◽  
Measuring Cell ◽  
Optical Alignment

scholarly journals Versatile and High-throughput Force Measurement Platform for Dorsal Cell Mechanics

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Seungman Park ◽  
Yoon Ki Joo ◽  
Yun Chen
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Force Measurement ◽  
Time Lapse ◽  
Dorsal Side ◽  
Single Particle Tracking ◽  
Single Experiment ◽  
Molecular Binding ◽  
Force Magnitude

Abstract We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted on the cell can be modulated ranging from ~14 pN to 2 nN to perturb various force-dependent processees in cells. Time-lapse images were acquired to record events due to such perturbation. The values of various mechanical parameters are subsequently obtained by single particle tracking. Up to 50 events can be measured simultaneously in a single experiment. Integrating the microfluidic techniques with the analytic framework established in computational fluid dynamics, our method is physiologically relevant, reliable, economic and efficient.

Measuring Cell Mechanics

2015 ◽  
Vol 2 (1) ◽  
pp. 1-75 ◽  
Author(s):  
Margaret Gardel ◽  
Patrick Oakes
Keyword(s):  
Cell Mechanics ◽  
Measuring Cell

High-throughput measurements of cell mechanics using atomic force microscopy with micro-patterned substrates

MHS2013 ◽  
2013 ◽  
Author(s):  
Ryosuke Takahashi ◽  
Kaori Kuribayashi-Shigetomi ◽  
Masahiro Tsuchiya ◽  
Agus Subagyo ◽  
Kazuhisa Sueoka ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Throughput ◽  
Cell Mechanics ◽  
Patterned Substrates ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals High-throughput ballistic injection nanorheology to measure cell mechanics

2012 ◽  
Vol 7 (1) ◽  
pp. 155-170 ◽  
Author(s):  
Pei-Hsun Wu ◽  
Christopher M Hale ◽  
Wei-Chiang Chen ◽  
Jerry S H Lee ◽  
Yiider Tseng ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Mechanics

scholarly journals Versatile and High-Throughput Microfluidics Platform for Dorsal Cell Mechanics

2019 ◽  
Vol 116 (3) ◽  
pp. 121a
Author(s):  
Seungman Park ◽  
Yoon Ki Joo ◽  
Yun Chen
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Microfluidics Platform

scholarly journals An Automated High-throughput Array Microscope for Cancer Cell Mechanics

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jeremy A. Cribb ◽  
Lukas D. Osborne ◽  
Kellie Beicker ◽  
Matthew Psioda ◽  
Jian Chen ◽  
...  
Keyword(s):  
Cancer Cell ◽  
High Throughput ◽  
Cell Mechanics
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