scholarly journals Validation of a Field Technique and Characterization of Fecal Glucocorticoid Metabolite Analysis in Wild Chimpanzees (Pan troglodytes)

2012 ◽  
Vol 75 (1) ◽  
pp. 57-64 ◽  
Author(s):  
CARSON M. MURRAY ◽  
MATTHEW R. HEINTZ ◽  
ELIZABETH V. LONSDORF ◽  
LISA A. PARR ◽  
RACHEL M. SANTYMIRE
2001 ◽  
Vol 15 (1) ◽  
pp. 69-76 ◽  
Author(s):  
A. Ramdani ◽  
B. Cros ◽  
M. Sidki ◽  
J.-Y. Ferrandis

2011 ◽  
Vol 17 (S2) ◽  
pp. 1100-1101
Author(s):  
R Hūbner ◽  
H-J Engelmann ◽  
E Zschech

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.


Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 517 ◽  
Author(s):  
Younes Laidoudi ◽  
Hacène Medkour ◽  
Maria Stefania Latrofa ◽  
Bernard Davoust ◽  
Georges Diatta ◽  
...  

Abbreviata caucasica (syn. Physaloptera mordens) has been reported in human and various non-human primates including great apes. The identification of this nematode is seldom performed and relies on egg characterization at the coproscopy, in the absence of any molecular tool. Following the recovery of two adult females of A. caucasica from the feces of wild Senegalese chimpanzees, morphometric characteristics were reported and new data on the width of the esophagus (0.268–0.287 mm) and on the cuticle structure (0.70–0.122 mm) were provided. The molecular characterization of a set of mitochondrial (cox1, 16S rRNA, 12S rRNA) and nuclear (18S rRNA and ITS2) partial genes was performed. Our phylogenetic analysis indicates for the first time that A. caucasica is monophyletic with Physaloptera species. A novel molecular tool was developed for the routine diagnosis of A. caucasica and the surveillance of Nematoda infestations. An A. caucasica-specific qPCR targeting the 12S gene was assessed. The assay was able to detect up to 1.13 × 10−3 eggs/g of fecal matter irrespective of its consistency, with an efficiency of 101.8% and a perfect adjustment (R2 = 0.99). The infection rate by A. caucasica in the chimpanzee fecal samples was 52.08%. Only 6.19% of the environmental samples were positive for nematode DNA and any for A. caucasica. Our findings indicate the need for further studies to clarify the epidemiology, circulation, life cycle, and possible pathological effects of this infestation using the molecular tool herein developed.


2012 ◽  
Vol 49 (2) ◽  
pp. 710-719 ◽  
Author(s):  
Andreia P. Oliveira ◽  
Paula Baptista ◽  
Paula B. Andrade ◽  
Fátima Martins ◽  
José A. Pereira ◽  
...  

2005 ◽  
Vol 120 (1) ◽  
pp. 94-100 ◽  
Author(s):  
Abderrafi Ramdani ◽  
Mouncif Sidki ◽  
Hassan Rhmini ◽  
Jean-Yves Ferrandis

2013 ◽  
Vol 122 ◽  
pp. 72-75 ◽  
Author(s):  
D. Ramos ◽  
A. Reche-Junior ◽  
P.L. Fragoso ◽  
R. Palme ◽  
N.K. Yanasse ◽  
...  

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