Fabrication of a poly(ɛ-caprolactone)/starch nanocomposite scaffold with a solvent-casting/salt-leaching technique for bone tissue engineering applications

2016 ◽  
Vol 133 (23) ◽  
Author(s):  
Safa Taherkhani ◽  
Fathollah Moztarzadeh
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Solvent Casting ◽  
Engineering Applications ◽  
Salt Leaching ◽  
Nanocomposite Scaffold

Poly(lactic-co-glycolic)/Nanostructured Merwinite Porous Composites for Bone Tissue Engineering. I. Preparation and Morphology

2011 ◽  
Vol 493-494 ◽  
pp. 718-722 ◽  
Author(s):  
Masoud Hafezi-Ardakani ◽  
Faranak Kavian ◽  
Fatollah Moztarzadeh ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Ali Zamanian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Weight Loss ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Solvent Casting ◽  
Salt Leaching ◽  
Uniform Microstructure ◽  
Porous Composites ◽  
Phosphate Buffered Saline ◽  
Interconnected Pores

A novel merwinite/ Poly(lactic-co-glycolic) nanocomposite was synthesized by a solvent casting/salt leaching technique with varying merwinite contents from 10 to 30% (w/w). Poly(lactic-co-glycolic) /merwinite foams with a co-continuous structure of interconnected pores were formed. The microstructure of the pores and the walls was controlled by varying the merwinite content. The pore structure becomes more and more irregular with increasing merwinite content. Pore sizes ranging from several microns to a few hundred microns were obtained. The degradation assessment of the scaffolds is performed in phosphate-buffered saline (PBS) solution at 37°C. Weight loss during storage at 37°C in PBS (pH 7.4) was determined for the scaffolds. Weight loss increased from pure to high content during incubation time. The prepared merwinite/ (Polylactic-co-glycolic) nanocomposite with uniform microstructure may be used in bone tissue engineering applications.

Polylactic Acid Scaffold Fabricated by Combination of Solvent Casting and Salt Leaching Techniques: Influence of β-Tricalcium Phosphate Contents

2017 ◽  
Vol 264 ◽  
pp. 42-45
Author(s):  
Rosaniza Md Isa ◽  
Mariatti Jaafar
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polylactic Acid ◽  
Tricalcium Phosphate ◽  
Three Dimensional ◽  
Combination Method ◽  
Solvent Casting ◽  
Salt Leaching

β-tricalcium phosphate (β-TCP) is a ceramic that commonly been used in bone tissue engineering. This material exhibits low mechanical properties such as low fracture toughness and brittleness. To overcome these problems, polylactic acid (PLA)/ β-TCP scaffolds for bone tissue engineering were prepared by using the combination method of solvent casting and salt leaching. These methods were used to produce three-dimensionally interconnected pores of the scaffold according to different ratio of β-TCP with porogen agent (sodium chloride (NaCl)). It is found that porosity and pore size of the scaffolds were independently controlled by the ratio and the particle size of the added porogen. The increases of pore interconnectivity were observed with increasing of PLA/ β-TCP/ NaCl ratios. Scaffolds with 80-90 wt% of total porogen content displayed acceptable mechanical properties for bone tissue engineering applications. Morphology observed by scanning electron microscopy (SEM) revealed that highly porous three-dimensional scaffold (>80 wt%) with well interconnected porous structure could be achieved by this combination process.

scholarly journals A Proposed Fabrication Method of Novel PCL-GEL-HAp Nanocomposite Scaffolds for Bone Tissue Engineering Applications

2010 ◽  
Vol 19 (4) ◽  
pp. 096369351001900 ◽  
Author(s):  
A. Hamlekhan ◽  
M. Mozafari ◽  
N. Nezafati ◽  
M. Azami ◽  
H. Hadipour
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Test ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fabrication Method ◽  
Engineering Applications ◽  
Poly Caprolactone

In this study, poly(∊-caprolactone) (PCL), gelatin (GEL) and nanocrystalline hydroxyapatite (HAp) was applied to fabricate novel PCL-GEL-HAp nanaocomposite scaffolds through a new fabrication method. With the aim of finding the best fabrication method, after testing different methods and solvents, the best method and solvents were found, and the nanocomposites were prepared through layer solvent casting combined with freeze-drying. Acetone and distillated water were used as the PCL and GEL solvents, respectively. The mechanical test showed that the increasing of the PCL weight through the scaffolds caused the improvement of the final nanocomposite mechanical behavior due to the increasing of the ultimate stress, stiffness and elastic modulus (8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL). The biomineralization investigation of the scaffolds revealed the formation of bone-like apatite layers after immersion in simulated body fluid (SBF). In addition, the in vitro cytotoxity of the scaffolds using L929 mouse fibroblast cell line (ATCC) indicated no sign of toxicity. These results indicated that the fabricated scaffold possesses the prerequisites for bone tissue engineering applications.

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